• The Journal of Korean Medicine
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• v.18 no.1
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• pp.126-142
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• 1997

For last four or five years, the number of Korean students have gone on increasing awfully. After the year 1997 when they will flow in, our field and government will be undergone a big shock and social problems. Therefore, thorough grasping and the method of provision is expeced to need. 1. The situation, According to the data published from the year 1993 to 1997, their results are great different. But recent Korean embassy in China examined the situation of the Korean students, whose result was investigated as follows: about 1500 persons, their average age is 24 to 44, the ratio of male and female students is 3 to 1, that of their academic background which is a graduate of university : that of college : that of highschool is 1.6 : 1 : 4.7, and that of their class attitude which is direct : common : indirect appears the percents 30.4 : 52.8 : 16.8. 2. The controversial points (1) A lowering of standards on Chinese medical treatment is expected by the imprudent studying abroad without qualification. And because of excessive production of herb doctors, the amount of treatment may be above people's demand. Besides owing to the impossibility of prediction on the number the counterplans of our government may fall into utler confusion. (2) The curriculum, the teaching schedule and contents are so different that the graduates seem not to be given real education of chinese medicine from the point of Korean view. (3) In the wrong national examination, the important thought of china, and the system of WTO in Korea, when China will become mighty rival above problems contain the negative sides. 3. The counterplans - what should be performed soon. (1) Illegal contents about studying abroadl in China ought to inform sidely that students might not go on increasing. Before they form nam-power, the government and the field of chinese medicine should cope with such a situation together. (2) The law of regulation about agents should be legislated and they should be surpervised. (3) The exact numbers of students, their situation, and the present condition of each university should be examined. (4) After investigating the problems of the present system of national test, its enforcement and many counterplans is requisite. (5) The cooperating confrontation with the conference of dentistry is in need. (6) Since the problems are possibility to be developed to diplomatic questions, the effort of both nations is needful. - what need middle of long time. (1) The effort that suppress the more establishment of the college of oriental medicine is necessary. (2) After comparing with the system of foreign test, the government should make the system of test in accord with the mutualism and the principle of indiscrimination among nations and cope with such a many issues thoroughly.

• Journal of Life Science
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• v.30 no.11
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• pp.973-982
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• 2020

In this study, we examined inhibition of adipocyte differentiation associated with the administration of camphor, a substance identified in extracts of the flowering plant Chrysanthemum indicum L. (CI). No camphor-mediated cytotoxicity was observed over a period of 1-10 days in studies targeting cells of the 3T3-L1 adipocyte-like line. Experiments that featured siRNA-mediated suppression of the transmembrane proteins Patched (PTCH) and Smoothened (SMO) resulted in inhibition and activation of differentiation, respectively. Interestingly, inhibition of PTCH typically activates SMO protein targeting and serves to activate hedgehog (HH)-mediated signaling. The results of our study suggest that activation of HH-mediated signaling can inhibit adipocyte differentiation. Furthermore, expression of glioma-associated oncogene homologue 1 (Gli1) was detected by flow cytometry in 62.7±1.5% of cells in response to administration of Lactobacillus rhamnosus (KCTC 3237) and in 60.4±2.2% of cells in response to camphor; these levels are higher than those detected in undifferentiated controls (24.9±3.1%). No change in the state of fermented camphor was identified by gas chromatography-mass spectrometry (GC-MS), but a 15.41% quantitative increase was confirmed in KCTC 3237. Overall, we conclude that administration of camphor resulted in overexpression of SMO and modulated the differential expression of Gli1. Animal studies focused on the impact of camphor as an agent to counteract obesity might be considered in the future. Indeed, camphor and similar physiologically active compounds from fermented CI might be developed as new and effective treatments for obesity.

• Journal of the Korean association of regional geographers
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• v.10 no.4
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• pp.757-774
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• 2004

This paper is to reclassify systematically the functions of urban river: that is, water supply, land management, transportation and energy source as social and economic function; formation of geomorphic surface, water-side landscape, community constitution and boundary and separation between regions as spatial function; and drainage of waste water, purification, habitation, and weather regulation as ecological function. On the basis of this reclassification, it can be argued that the socio-economic functions (eg. water supply) of the river among the functions of the river have been strongly mobilized in the process of modernization, while the spatial function and ecological function of urban river have been ignored. The Kumho river which flows through Daegu and the adjacent area has made a great contribution to the modem development process of the river basin area, but as a result of a selective development of a specific function of the river, that is the social and economic function, it now suffers from the lack of instream flow and is deprived of its original functions with the water pollution and degradation. Moreover the Daegu region seems no longer possible to develop on the dependence of the river. In order to overcome this kind of social and environmental crisis, this paper is to suggest both some principles and main evaluating indicators to restore the original and comprehensive functions of the river, and important measures to make the co-evolution of the city and the river possible.

• The Journal of the Korean bone and joint tumor society
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• v.13 no.2
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• pp.88-95
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• 2007

Purpose: The purpose of this study was to compare the proapoptotic effects of matrix metal-loproteinase inhibitor (MMPI) and doxorubicin on wild-type p53 osteosarcoma cell line, socalled U2OS cell line. Materials and Methods: U2OS cells were treated with MMP inhibitor III (MMPI III) and doxorubicin, either respectively or simultaneously. In cells treated with doxorubicin, Fas-neutralizing antibody so called ZB4 was additionally treated to examine whether the doxorubicin played a role through the Fas/FasL pathway. Cells were analysed regarding to apoptosis and cell death by flow cytometry. Results: U2OS cells incubated with doxorubicin showed significant amount of cell death in dose-dependent manner. However, those incubated with MMPI III mostly remained viable state. In addition, there is no relationship between two drugs. Cells treated with doxorubicin and ZB4 at the same time did not show down regulation of apoptosis through inhibition of Fas/FasL pathway. Conclusion: It is important to re-examine MMP inhibitor's effect on other osteosarcoma cell line with wild-type p14 as well as wild-type p53 to evaluate its proapoptotic effect.

• Journal of Environmental Science International
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• v.16 no.10
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• pp.1179-1187
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• 2007

This paper represents the statistical characteristics of sources and receptors for road traffic noise in apartment complex. Also we suggested that the site-specific characteristics of vertical and horizontal distributions in the complex apartment have been derived using a different analysis for evaluating levels of Leq1h by the apartment floor at a given distance from a road in terms of the flow rate, the mean speed of the traffic, and the percentage of the type vehicles in the day and night periods. As a result, the contribution orders of traffic quantity by the type of vehicles showed as followed: light vehicle>medium vehicle>heavy vehicle>motorcycle. Especially, the mixing ratio of entering the road on the heavy vehicle in the daytime was two times higher than that of in the night. The speed in all types of vehicle is in the range of 41 and 81 km/hr and noise level was not significantly different in day and night-time. The sources of road traffic noise had different variations and uncertainties using a random variable and probability distribution. The sound distribution to receptors by the apartment floor showed S curve between 1st floor and $15^{th}$ floor With the normality test, the normal distributions using Anderson-Darling Test followed $1^{st},\;3^{th},\;13^{th}$ and $15^{th}$, floor in the daytime and all floors except $7^{th}$ floor in the night (p>0.05). And also, the value of the pearson correlation coefficient (r) obtained in this study have significantly different at the range of floor. In conclusion, the results suggested that the distribution characteristics of levels of $Leq_{1h}$ on the sources and receptors of road traffic noise in apartment complex must be improved and developed on the guideline of regulation of road traffic noise.

• Journal of Navigation and Port Research
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• v.33 no.5
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• pp.315-321
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• 2009

The Very Large Marine Structure has been widely used new method of ocean space instead of method for reclamation Therefore, VLFS is proposed to coincide on such request. It can be established regardless of nature of soil and height of water, and stream of flow exists under the floating structure, there is seldom effect in natural environment. Fuertherrnore, it can do easily to do assembly and taking to pieces due to expansion or removal. Based on the regulation by class, VLFS have to possess more than enough structural strength against severe wave loading induced by green sea condition Therefore, There are performed structural simulation as well as experimental test about expected loading scenario in order to examine the safety of structure. Up to now, various examinations based on the strength limit value of the main structural material have been done based on the elasticity response analysis. However, there is little finding about the collapse behavior and the safety when the load that exceeds the collapse of the material acts. In the present study, we investigated the collapse behavior based on the ultimate limit state calculated by FE-analysis.

• IMMUNE NETWORK
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• v.7 no.3
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• pp.133-140
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• 2007

Background: It is well established that cross talk between natural killer (NK) cells and myeloid dendritic cells (DC) leads to NK cell activation and DC maturation. In the present study, we investigated whether type 1-polarized DC (DC1) matured in the presence of IFN-${\gamma}$ and type 2-polarized DC (DC2) matured in the presence of PGE2 can differentially activate NK cells. Methods: In order to generate DC, plastic adherent monocytes were cultured in RPMI 1640 containing GM-CSF and IL-4. At day 6, maturation was induced by culturing the cells for 2 days with cytokines or PGE2 in the presence or absence of LPS. Each population of DC was cocultured with NK cells for 24 h. The antigen expression on DC was analyzed by flow cytometry and cytokine production in culture supernatant was measured by ELISA or a bioassay for TNF-${\alpha}$ determination. NK cell-mediated lysis was determined using a standard 4h chromium release assay. Results: DC2, unlike DC1, had weak, if any, ability to induce NK cell activation as measured by IFN-${\gamma}$ production and cytolytic activity. DC2 were weakly stimulated by activated NK cells compared to DC1. In addition, IFN-${\gamma}$-primed mature DC appeared to be most resistant to active NK cell-mediated lysis even at a high NK cell/DC ratio. On the other hand, PGE2-primed DC were less resistant to feedback regulation by NK cells than IFN-${\gamma}$-primed mature DC. Finally, we showed that the differential effect of two types of DC population on NK cell activity is not due to differences in their ability to form conjugates with NK cells. Conclusion: These results suggest that different combinations of inflammatory mediators differentially affect the effector function of DC and, as a result, the function of NK cells, eventually leading to distinct levels of activation in adaptive immunity.

• The Korea Journal of Herbology
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• v.25 no.2
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• pp.129-136
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• 2010

Objective : Sinapis alba L. (SA) is a korean traditional herbal medicine that is usually used to prevent or treat inflammatory diseases, such as respiratory infection and rheumatoid arthritis. However, the effects of SA supplementation in vitro on serum antibody levels, splenocyte and peritoneal macrophage immune responses have not yet been determined. In this study, we examined the effect of SA on the production of Th1/Th2 cytokines. Methods : Splenocytes were isolated from naive C57BL/6 mice. Cells were enriched for CD4+ cell populations by first staining the cells with anti-CD4 (BD PharMingen, Calif, USA). CD4+ T cells were selected on a (CS) column, and the flow-through was collected as CD4+ T cells. Isolated cells were activated by overnight incubation on 24-well plates coated with $1{\mu}g/mL$ anti-CD3, $1{\mu}g/mL$ anti-CD28 and with SA ($100{\mu}g/mL$). Primary macrophages were collected from the peritoneal cavities of mice (8-week-old female C57BL/6). The peritoneal macrophages were washed and plated with RPMI-1640 overnight for the experiments. After 48-hours cultures, samples were centrifuged at 2000 rpm for 10 minutes, and the supernatants were stored at $-80^{\circ}C$. Mouse IL-4, IFN-$\gamma$ and TNF-$\alpha$ were quantified using ELISA kits (BioSource International, Camarillo, Calif, USA) according to the manufacturer's protocols. Results : SA at 100ug/ml decreased the generation of Th1 cytokine (IFN-$\gamma$) by 0.5-fold. However, SA has no effect on Th2 (IL-4) production. Conclusions : These results suggest that SA may play an important role in the control of T-cell-mediated autoimmunity by down-regulation of Th1 cytokine (especially IFN-$\gamma$, TNF-$\alpha$). These data may contribute to the design of new immunomodulating treatments for a group of autoimmune diseases.

• Management & Information Systems Review
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• v.31 no.4
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• pp.33-56
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• 2012

The purposes of this paper are analysed the relationship between environmental regulation and financial performance in Korea. The financial performance of listed companies and Outside Auditing firms was evaluated by logistic regression. First, R&D investment is proportionally correlated with the ability to comply with environmental regulations. It can be explained increase of investment in R&D causes enhancement of compliance of environmental regulations with development of environmental technology. Second, statistical significance is not observed between financial aspects such as current ratio, debt-to-equity ratio, cash flow, and operating profit margin and the ability to comply with environmental regulations. It indicates high financial performance could not directly lead investment for the ability of that. Third, in terms of structural aspects, firm size and employees have a reliable correlation with compliance with environmental regulations due to high attention of larger firms for PR, and IR, while capital intensity and gravity, and exports do not. Finally, violation of environmental regulations is not affect by the controlling shareholder ownership.

• Clinical and Experimental Reproductive Medicine
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• v.37 no.3
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• pp.181-189
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• 2010

The placenta, which is a temporary organ derived from the fetus during pregnancy, is critical to support fetus development via optimal regulation between mother and fetus. Trophoblast as a major cell population of the placenta is one of the earliest to differentiate and shows an extensive proliferation or/and differentiation up to the formation of the placenta. The role of the trophoblast show dynamic changes from early embryo implantation to placentation during pregnancy. Implantation of the blastocyst into the endometrium of the maternal uterus is mediated by invasion of the differentiated trophoblast (e.g. syncytiotrophoblast) from the trophectoderm. During pregnancy, the unique role of the trophoblast is to invasion, eroding, and metastasizing in the placenta as well as to ensure appropriate bidirectional nutrient or waste flow required for growth and maturation of the embryo. The dysfunction of the trophoblast during pregnancy can result in several gynecological diseases including preeclampsia and congenital malformation in neonatal medicine. Therefore, trophoblasts act as a conclusive factor in placental and fetal development. This brief review outlines the classification of trophoblast and its function in the placenta during pregnancy. Also, we introduce the latest research in trophoblast for implantation and the placenta development, and the application potential of trophoblast for infertility and obstetrical diseases.