• Journal of Embryo Transfer
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• v.30 no.3
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• pp.121-128
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• 2015

The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed ($37^{\circ}C$) thermos bottle by gloved-hand method and was separated by 65% Percoll with superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) before freezing. The frozen sperm was thawed at $38.5^{\circ}C$ for 45 sec in water-bath for sperm characteristic analysis. The sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction, Rhodamine123/PI double staining for mitochondrial integrity and were analyzed using flow cytometry. In results, sperm viability, acrosome reaction and mitochondrial integrity were improved in separated sperm groups compared with unseparated sperm by Percoll (UP) group. Especially, viability was significantly higher in sperm separated by Percoll containing 400 IU CAT group compared with other groups (P<0.05). And acrosome reaction was decreased in sperm separated by Percoll with 300 IU SOD, 400 IU CAT and 0.5 mM GSH groups compared with other groups, however, there were no significantly difference mitochondrial integrity among sperm separated by Percoll with antioxidant enzymes. In conclusion, we suggest that use of Percoll containing antioxidant enzymes for sperm separation will be beneficial for sperm cryopreservation in pigs.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.02a
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• pp.44-45
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• 2010

We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a $CO_2$-incubator. The small animals used in the experiment involving plasma irradiation into living tissue were rat, rabbit, and pick and are deeply anesthetized with the gas anesthesia. According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was irradiated. Furthermore, in an experiment testing the effects of plasma irradiation on rats that were artificially given burn wounds, no evidence of electric shock injuries was found in the irradiated areas. In fact, the observed evidence of healing and improvements of the burn wounds suggested the presence of healing effects due to the growth factors in the tissues. Therefore, it appears that the interaction due to ion/radicalcollisions causes a substantial effect on the proliferation of growth factors such as epidermal growth factor (EGF), nerve growth factor (NGF), and transforming growth factor (TGF) that are present in the cells.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.318-327
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• 2007

This study presents the molecular phylogenetic analysis of Korean Aurelia aurita Linnaeus collected from Yeosu in the southern waters of Korea using nuclear ITS1 region and mitochondrial COI gene sequences. The use of oligonucleotide primers F5 (forward) and R5 (reverse) targeted to ITS1 and LCO1490 (forward) and HCO2198 (reverse) targeted to COI amplified 267 bp and 643 bp fragments, respectively. The shortest genetic distance towards the ITS1 region is estimated at 0.023 when comparing Korean A. aurita to Aurelia sp. collected from California, USA. In particular, Korean and American/Swedish A. aurita were located far away in terms of genetic distance, ranging from 0.393 to 0.395. On the other hand, the genetic distance between Korean and English/Turkish/Swedish/American A. aurita regarding the mitochondrial DNA COI gene ranged from 0.201 to 0.205. However, a sister-ship with Korean and American A. aurita showed an extremely high bootstrap value (100%). The predicted secondary RNA structure of the mitochondrial DNA COI gene showed many different folding structures with a similar energy between Korean and American A. aurita. These results suggest that ITS1 and the mitochondrial DNA COI gene could be used as genetic markers for identification of the biogeographic populations.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.5
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• pp.2844-2858
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• 2014

The society experiencing the industrialization and urbanization has got over the socio-demographic change. these changes make the number of the population around the world, and this phenomenon is flowing into the whole country. Korea has became a Aging Society since 2000 and will be turned into the aged society by the 2018. therefore, the importance of preparing elderly living life such as silver town is getting emphasized. the purpose of this study is aimed at analyzing the decision elements of the preliminary demanders' intention who selecting Elderly Housing. Based on this study, it was broken down by the type much more. Binary Logistic Regression Analysis of Factors affecting the Elderly housing choices were subdivided. Through this process, improvement and the implications of this study was derived. this study deducts 3 kinds of implications. First, the preference for the development of elderly housing are different with each type of characteristics. Second, the indicators along with the individual characteristics account for the most part of the surface. so the specific investigation for the demand must be required to check the indicators. Third, when it comes to development of urban elderly housing, it requires to have a part of a local government plans securing the land. Fourth, when it comes to development of suburb elderly housing, it is required to arrange the living environment around the suburbs to let user classes living in Gyeonggi-do flow into elderly housing and live their new-life in the suburbs. Finally, when it comes to development of rural elderly housing, a variety of production, leisure and other programs should be made and put into there.

• Korean Journal of Environment and Ecology
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• v.32 no.6
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• pp.591-602
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• 2018

Clithon retropictus has been designated as an endangered wildlife Class II due to its high value as a biological indicator species capable of judging environmental quality such as salinity, water flow, and ground conditions. However, basic research on its physiological and ecological characteristics is still lacking. As such, this study intended to examine the impact of environmental conditions such as salinity and soil particle size on the size and density of Clithon retropictus at the Yeoncho river estuary. The investigation of the salinity, which is a key variable that affects the distribution of organisms in the estuary, showed that Clithon retropictus could grow at a salinity ranging from 0#x2030; (freshwater) to 25‰ (brackish water). The coarse gravel (19-75mm) tended to increase nearer the upper stream (under the Yeoncho weir), while the proportion of particles smaller than sand (less than 19mm) increased toward the downstream. The population and the size of the individuals decreased rapidly in the downstream where water stagnated near the Yeoncho weir, and the salt water joined. The results indicated that Clithon retropictus had a high tolerance to salinity, but the adaptability was weaker toward the extremes since the population, and the size tended to decrease as the salinity increased. The correlation analysis revealed that both salinity and soil particle size affected the population and individual size. The correlation between the individual size and salinity was -0.242 (P <0.01), indicating that the size decreased with increasing salinity. The correlation between individual size and coarse gravel having a particle size of 19mm or more was 0.420 (P <0.01), indicating that the size increased with increasing the particle size.

• Journal of Wetlands Research
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• v.21 no.3
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• pp.244-250
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• 2019

Due to the industrial development and population growth, it has recently been shown that there are many problems caused by the rinked treatment water in local goverments and sewage treatment plants. The rinked treatment water has a characteristic of low flow rate and high concentration unlike general sewage. These characteristics increase sewage treatment difficulty and sewage treatment fee of sewage treatment facilities. Among the many influencing factors that increase sewage treatment unit cost, 'linked treatment load/design inflow load (%)' was derived as the most correlated factor. Through the selection and modeling of sewage treatment plants, the excess scope of design discharge water quality was investigated under the conditions of temperature and the conditions of 'linked treatment load/design inflow load (%)' taking into account the effects of the four seasons. The study found that for TN, 'linked treatment load/design inflow load (%)' was 19.7%, 22.6%, 25.1%and 27.7%, respectively, under conditions of $5^{\circ}C$, $10^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$. In case of TP, 'rinked treatment load/design inflow load (%)' was 10.7%, 12.2%, 15.6% and 17.5% at $5^{\circ}C$, $10^{\circ}C$, $20^{\circ}C$, and $25^{\circ}C$, respectively, under conditions of $5^{\circ}C$, $10^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.32 no.1
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• pp.37-44
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• 2019

A bond-based peridynamic model has been reported dynamic fracture characteristic of brittle materials through a simple constitutive model. In the model, each bond is assumed to be a simple spring operating independently. As a result, this simple bond interaction modeling restricts the material behavior having a fixed Poisson's ratio of 1/4 and not being capable of expressing shear deformation. We consider a state-based peridynamics as a generalized peridynamic model. Constitutive models in the state-based peridynamics are corresponding to those in continuum theory. In state-based peridynamics, thus, the response of a material particle depends collectively on deformation of all bonds connected to other particles. So, a state-based peridynamic theory can represent the volume and shear changes of the material. In this paper, the perfect plasticity is considered to express plastic deformation of material by the state-based peridynamic constitutive model with perfect plastic flow rule. The elastic-plastic behavior of the material is verified through the stress-strain curves of the flat plate example. Furthermore, we simulate the high-speed impact on 3D granite model with a nonlocal contact modeling. It is observed that the damage patterns obtained by peridynamics are similar to experimental observations.

• Ecology and Resilient Infrastructure
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• v.5 no.4
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• pp.246-256
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• 2018

In order to analyze hydraulic characteristics of discharge coefficient, hydraulic jump height, and hydraulic jump length, accompanied sediment transport, in the under-flow type vertical lift gate, the hydraulic model experiment and dimensional analysis were performed. The correlations between Froude number and hydraulic characteristics were schematized according to the presence and absence of sediment transport; the correlation of hydraulic characteristics and non-dimensional parameters was analyzed and multiple regression formulae were developed. In the hydraulic characteristics accompanied the sediment transport, by identifying the aspect different from the case that the sediment transport is absent, we verified that it is necessary to introduce variables that can express the characteristics of sediment transport. The multiple regression equations were suggested and each determination coefficient appeared high as 0.749 for discharge coefficient, 0.896 for hydraulic jump height, and 0.955 for hydraulic jump length. In order to evaluate the applicability of the developed hydraulic characteristic equations, 95% prediction interval analysis was conducted on the measured and the calculated by regression equations, and it was determined that NSE (Nash-Sutcliffe Efficiency), RMSE (root mean square), and MAPE (mean absolute percentage error) are appropriate, for the accuracy analysis related to the prediction on hydraulic characteristics of discharge coefficient, hydraulic jump height and length.

• Journal of Korea Water Resources Association
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• v.52 no.9
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• pp.615-626
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• 2019

Soil moisture and runoff have very close relationship. Especially the water retention capacity and drainage characteristics of the soil are determined by various factors of the soil. In this study, a total of 40 rainfall events were identified from the entire rainfall events of Sulma basin in 2016 and 2017. For each selected events, the constant-K method was used to separate direct runoff and baseflow from total flow and calculate the runoff coefficient which shows positive exponential curve with Antecedent Soil Moisture (ASM). In addition to that, the threshold of soil moisture was determined at the point where the runoff coefficient starts increasing dramatically. The threshold of soil moisture shows great correlation with runoff and depth to water table. It was founded that not only ASM but also various factors, such as Initial Soil Moisture (ISM), storage capacity of soil and precipitation, affect the results of runoff response. Furthermore, wet condition and dry condition are separated by ASM threshold and the start and peak response are analyzed. And the results show that the response under wet condition occurred more quickly than that of dry condition. In most events occurred in dry condition, factors reached peak in order of soil moisture, depth to water table and runoff. However, in wet condition, they reached peak in order of depth to water table, runoff and soil moisture. These results will help identify the interaction among factors which affect the runoff, and it will help establish the relationship between various soil conditions and runoff.

• The Korean Journal of Archival Studies
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• no.37
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• pp.75-142
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• 2013

This Study is to propose the archival description in accordance with the paradigm shift of the archival science. The academic world of archival science in North America treated the electric record in 1990 and the authenticity of electric record in the beginning of 2000, the flow of Study recognizing the microscopic and the cultural discussion appeared recently. This may be called the paradigm shift of archival science. This may be according to the influence of the informatization and the technology. However, the community archives, the everyday life archives recognizing the microscopic and the cultural discussion, etc. Namely, the everyday life, the village, the community archives are the effort to leave the excluded and the neglected voices from the existing discussion of archival science on record. However, the currently existing methodology to archive the community archives based on the perception of these switched recognition is limited. Especially the description of the various standards being used in the public territory is being adapted as it is. The characteristic and the individuality of the community and the community archive cannot be reflected through the archival description standard. We should understand the community archive in accordance with the paradigm shift, need the archival description method capable of expressing this suitably. The post-standard not the existing archival description method has been necessary. This should be carried out together in accordance with the view point of the paradigm shift and the view point of the objective and the direction of community. Hence, first the archival description and the paradigm shift have been examined theoretically and the archival description of community archive being operated currently has been analyzed. The archival description is to be proposed in accordance with the paradigm shift capable of calling 'the archival description of Korean Human Right' by pointing out the limit of archival description standard through the analysis and further by investigating the archival description of the fraternity archive project.