• Transactions of The Korea Fluid Power Systems Society
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• v.4 no.4
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• pp.1-7
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• 2007

Flow rate is one of the important variables for precise motion control and detection of the faults and fluid loss in many hydraulic components and systems. But in many cases, it is not easy to measure it directly. The orifice area of a servo valve by which the fluid flows is one of key factors to monitor the flow rate. In this paper, we have constructed an estimation algorithm for the effective orifice area by using the model of a servo valve cylinder control system and Kalman filter algorithm. Without geometry information about the servo valve, it is shown that the effective orifice area can be estimated by using only displacement and pressure data corrupted with noise. And the effect of the biased sensor data and system parameter errors on the estimation results are discussed. The paper reveals that sensor calibration is important in accurate estimation and plausible parameter data such as oil bulk modulus and actuator volume are acceptable for the estimation without any error. The estimation algorithm can be used as an useful tool for detecting leakage, monitoring malfunction and/or degradation of the system performance.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.4
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• pp.561-569
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• 2002

This paper presents an investigation on $C_2$, CH, OH radicals and NOx emissions in partially premixed flames with acoustic excitation. The radicals are visualized by the digital image technique with optical filters and ICCD camera while NOx emissions are determined by a chemiluminescent detection(NOx analyser). The measurements are made in flames with an overall equivalence ratio (${\phi}_o$) 0.5 and a center tube equivalence ratio(${\phi}_c$) varing from 1.1 to 5.0 for a constant fuel flow rate. In the case of excitation, the visual shape of the flame is changed from laminar to turbulent-like flames. Images of $C_2$, CH, and OH radicals resemble those of the flame appearances as the excitation phase is varied, and the radicals generated at the upstream are convected toward the downstream. It is inferred that the flame characteristics is affected by the flow characteristics of air-fuel mixture. In the case of acoustic excitation, OH radicals are much increased relative to unexcitation. From the radicals and flame visualization under acoustic excitation, the reduction of flame length affects the shorter residence time of center tube mixture, and significantly influences the NOx reduction.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.895-901
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• 2013

Resistance to induction of apoptosis is a major obstacle for bladder cancer treatment. Bcl-2 is thought to be involved in anti-apoptotic signaling. In this study, we investigated the effect of Bcl-2 overexpression on apoptotic resistance and intracellular reactive oxygen species (ROS) generation in bladder cancer cells. A stable Bcl-2 overexpression cell line, BIU87-Bcl-2, was constructed from human bladder cancer cell line BIU87 by transfecting recombinant Bcl-2 [pcDNA3.1(+)-Bcl-2]. The sensitivity of transfected cells to adriamycin (ADR) was assessed by MTT assay. Apoptosis was examined by flow cytometry and acridine orange fluorescence staining. Intracellular ROS was determined using flow cytometry, and the activities of superoxide dismutase (SOD) and catalase (CAT) were also investigated by the xanthinoxidase and visible radiation methods using SOD and CAT detection kits. The susceptibility of BIU87-Bcl-2 cells to ADR treatment was significantly decreased as compared with control BIU87 cells. Enhanced expression of Bcl-2 inhibited intracellular ROS generation following ADR treatment. Moreover, the suppression of SOD and CAT activity induced by ADR treatment was blocked in the BIU87-Bcl-2 case but not in their parental cells. The overexpression of Bcl-2 renders human bladder cancer cells resistant to ADR-induced apoptosis and ROS might act as an important secondary messenger in this process.

• Journal of the Korean Society of Visualization
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• v.13 no.1
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• pp.35-42
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• 2015

CD4+ T-cell count determines the effectiveness for antiretroviral therapy (ART) in patients with human immunodeficiency virus (HIV). Although ART slows the progression of HIV to AIDS, rapid counting of CD4+ T lymphocytes with a drop of patient's blood sample is urgently needed to ensure timely ART treatment in rural areas. Recently point-of-care CD4 testing devices have been developed by using non-flow based imaging cytometer incorporated with a sample cartridge where CD4+ T cells are reacted with fluorescently tagged specific antibodies. Here we conducted an experimental study using a conventional fluorescence microscope-based imaging system to quantitate the interaction of CD4 antibodies with CD4+ T cells at different reaction conditions. We demonstrated that a fast and affordable point-of-care CD4 test is feasible with a far less amount of antibodies and a shorter incubation time compared with a conventional sample preparation protocol for flow cytometry. We also proposed a general method to evaluate and compare the detection limit across different CD4 counting platforms by using fluorescently labelled microbeads for intensity calibration.

• Korean Journal of Pharmacognosy
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• v.42 no.2
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• pp.138-143
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• 2011

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of two major flavonoid glycosides (poncirin and nanringin) in Poncirus trifoliata Raf. by different harvesting times and locations for two years. A SunFire $C_{18}$ column (4.6 mm${\times}$250 mm, 5 ${\mu}M$) was used at $40^{\circ}C$ for the determination of poncirin and naringin. The mobile phase using gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Flow rate was 1.0 mL/min and injection volume was 10 ${\mu}l$. The chromatogram was monitored by photodiode array (PDA) detection at 280 nm for the identification of two flavonoid glycosides in P. trifoliata. The contents of the two components in P. trifoliata ranged from 0.32~13.02%.

• Journal of Biosystems Engineering
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• v.27 no.6
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• pp.521-528
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• 2002

Rotary and plow implements are mainly utilized for the tillage operation in Korea, and a implement control system for agricultural tractors was designed and fabricated to improve the working accuracy and efficiency. The control system was composed of three units: 1) sensors fur detection of angle of liftarm, draft force, engine rpm, tillage depth and so on, 2) a controller, and 3) hydraulic circuits, which included solenoid valves and so on, for operation of three point linkage and implements. The control system can control the speed(high and low speed) of implements by adjusting input flow rates of the hydraulic cylinder which was controled by two speed valve, which was composed of a solenoid valve and a orifice. Indoor experiments were conducted to evaluate response characteristics of the designed implement control system under experimental conditions of various engine nm, two kinds of input flow rates of the cylinder and some input frequency. The results of experiments showed the response characteristics sufficient to use as the implement control system fur agricultural tractors.

• Nuclear Engineering and Technology
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• v.42 no.5
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• pp.546-551
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• 2010

The objective of this research is to estimate the crack location and size of a carbon steel pipe by using a laser ultrasound guided wave for the wall thinning evaluation of an elbow. The wall thinning of the carbon steel pipe is one of the most serious problems in nuclear power plants, especially the wall thinning of the carbon steel elbow caused by Flow-Accelerated Corrosion (FAC). Therefore, a non-destructive inspection method of elbow is essential for the nuclear power plants to operate safely. The specimens used in this study were carbon steel elbows, which represented the main elements of real nuclear power plants. The shape of the wall thinning was an oval with a width of 120mm, a length of 80mm, and a depth of 5mm. The L(0,1) and L(0,2) modes variation of the ultrasound guided wave signal is obtained from the response of the laser generation/air-coupled detection ultrasonic hybrid system represent the characteristics of the defect. The trends of these characteristics and signal processing were used to estimate the size and location of wall thinning.

• Journal of the Korean Institute of Telematics and Electronics
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• v.21 no.5
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• pp.33-40
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• 1984

In the conventional pulsed doppler system, gated CW is used to excite a ultrasonic transducer so that a group of linear RF amplifiers are required to excite a ultrasonic array transducer in the scanning pulsed doppler system. A pulsed doppler system without linear high voltage RF amplifiers, which excites the transducer impulsively, is studied theoretically and experimentally. In this paper, an experimental 8-channel pulsed doppler system is implemented, which uses quadrature defection to detect the direction of motion and can compensate the attenuation effect. The designed pulsed doppler system shows the possibility of real time multichannel doppler flow meter.

• Bulletin of the Korean Chemical Society
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• v.25 no.8
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• pp.1125-1129
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• 2004

This work assesses the potential of natural Analcime Zeolite as an adsorbent for preconcentration of lead (II) traces. Lead is quantitatively retained on 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol by column method with Analcime in the pH range of 5-6.5 and 2 mL $min^{?1}$ flow rate. Lead was removed from the column with 10.0 mL of 4 M hydrochloric acid and was determined by anodic stripping differential pulse voltammetry. 0.5ppb detection limit was obtained and linear dynamic range was 3 to $1.2{\times}10^5$ ppb in final solution with correlation coefficient of 0.999 and relative standard deviation of ${\pm}$ 1.2% (for eight replicate determination of 2.5 ${\mu}g\;mL^{?1}$ of lead). Various parameters such as the effect of pH, flow rate, instrumental conditions and interferences of some ions on the determination of lead have been studied in detail for optimization of conditions. The method was successfully applied for determination of lead in various samples.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.64 no.8
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• pp.1145-1153
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• 2015

The paper analyzes reactive power flow characteristic in power system by reactive power tracing. In addition, virtual buses are inserted in the algorithm to consider losses of transmission lines, and shunt capacitor treated as a reactive power generator. The results of simulation are analyzed by two points of view. The one is load’s point of view and another is generator’s point of view. Classic purpose of the reactive power tracing consists in the reactive power pricing. However, it is significantly used to select vulnerable area about line outage in this paper. To find the vulnerable area, reactive power tracing variations between pre-contingency and post-contingency are calculated at all load buses. In heavily load area, buses which has highest variation become the most vulnerable bus. This method is applied to the IEEE 39-bus system. It is compared with voltage variation result and VQ-margin to verify its effect.