• BMB Reports
• /
• v.46 no.5
• /
• pp.270-275
• /
• 2013

It is essential to analyze rare mutations in many fields of biomedical research. However, the detection of rare mutations is usually failed due to the interference of predominant wild-type DNA surrounded. Herein we describe a sensitive and facile method of detecting rare point mutation on the basis of allele-specific amplification in emulsion PCR. The identification and selective amplification of rare mutation are accomplished in one-pot reaction. The allele-specific primers coupled on magnetic beads allow the exclusive amplification and enrichment of the mutant amplicons. The productive beads bearing mutant amplicons are subsequently stained with the fluorescent dyes. Thus, the rare point mutations with a percentage as low as 0.1%, can be detected by fluorescent analysis. The relative percentages of mutation among different samples can be roughly accessed by counting the fraction of fluorescent positive beads through flow cytometry.

• Journal of Power Electronics
• /
• v.12 no.1
• /
• pp.120-127
• /
• 2012

Fault diagnosis technique of electrical drives is becoming more and more important, since voltage fed converter system has become industrial standard in many applications. Many studies have been conducted an inverter fault diagnosis for induction motors. However, there are few researches about fault diagnosis of 3-phase ac/dc PWM (Pulse Width Modulation) converter compared to the dc/ ac inverter. The ac/dc converter is the opposite of dc/ac inverter at current flow. Also, inverter and converter have different current patterns under the same condition of IGBT (Insulated gate bipolar transistor) open switch fault. Therefore, it is difficult to apply intact diagnosis methods of inverter to the converter. This paper proposes modified fault detection methods for IGBT open switch fault in 3-phase ac/dc PWM converter by modifying established fault diagnostic methods for dc/ac inverters.

• Journal of the Korean Chemical Society
• /
• v.29 no.4
• /
• pp.382-389
• /
• 1985

The effect of plasma operational parameters for the determination of rare earth elements(REE) by means of inductively coupled plasma(ICP) spectrometry was investigated. While the increase in the flow rate of carrier gas argon enhanced the sensitivity and lowered the detection limit, significant ionization interferences were observed. The decrease in RF power increased the signal to background ratio. The observation point showing the lowest ionization interference was slightly higher than the position where the spatial profile of the analyte reached the maximum. The detection limits of the spectral lines commonly used for the determination of REE were measured and the spectral lines relatively free from spectral interferences were chosen.

• Journal of Mechanical Science and Technology
• /
• v.19 no.3
• /
• pp.887-893
• /
• 2005

The aggregability of red blood cells (RBCs) was determined by laser backscattering light analysis in a microfluidic channel. Available techniques for RBC aggregation often adopt a rotational Couette-flow using a bob-and-cup system for disaggregating RBCs, which causes the system to be complex and expensive. A disposable microfluidic channel and vibration generating mechanism were used in the proposed new detection system for RBC aggregation. Prior to measurement, RBC aggregates in a blood sample were completely disaggregated by the application of vibration-induced shear. With the present apparatus, the aggregation indexes of RBCs can be measured easily with small quantities of a blood sample. The measurements with the present aggregometer were compared with those of LORCA and the results showed a strong correlation between them. The aggregability of the defibrinogenated blood RBCs is markedly lower than that of the normal RBCs. The noble feature of this design is the vibration-induced disaggregation mechanism, which can incorporate the disposable element that holds the blood sample.

• Journal of Korea Multimedia Society
• /
• v.23 no.11
• /
• pp.1383-1387
• /
• 2020

Recently, a 4-col 7-row LED Screen that provides various information of major roads and local governments has been installed and operated. However, due to deterioration due to changes in temperature and humidity, deterioration due to static electricity, and mechanical stress, partial module failure of the display may occur, which is a major cause of missing information of vitally given to citizens. However, there have been frequent cases where the 4-col and 7-row LED Screen that have failed due to reasons such as installed location where the signboards are installed on the road and outdoor, the lack of monitoring means at all times, and the lack of manpower is often neglected for a long time. Following this flow, this paper proposes a method to detect defective modules by analyzing the images collected through the camera fixed to the front part of the LED display.

• The Transactions of the Korean Institute of Electrical Engineers C
• /
• v.50 no.9
• /
• pp.444-449
• /
• 2001

In this paper, a novel ultraviolet sensor is presented based on a photoluminescent porous silicon. Porous silicon layer was formed by chemical etching of surface of pn junction in a $HF(48%)-HNO_3(60%)-H_20$ solution. Incident ultraviolet(UV) light is converted to visible light by photoluminescent porous silicon layer, and then this visible light generates electron-hole pairs in the pn junction, which produces a photocurrent flow through the device. In order to maximize detection efficiency, the peak sensitivity wavelength of the pn junction diode was matched with the peak wavelength of Photoluminescence from porous silicon layer. The porous silicon ultraviolet sensor showed a large output current as UV intensity increases and but very low sensitivity to visible light. The detection sensitivity of porous silicon sensor was calculated as 2.91mA/mW. These results are expected to open up a possibility that the present porous silicon sensor can be used for detecting UV light in a visible background, compared to silicon UV detectors which have an undesirable response to visible light.

• Nuclear Engineering and Technology
• /
• v.30 no.1
• /
• pp.8-16
• /
• 1998

In this work a method to detect the vibrational peak and to decide the vibrational mode of detected peak for core internal vibration monitoring system which is particularly concerned on the core support barrel (CSB) and fuel assemblies is developed. Flow induced vibration and aging process in the reactor internals cause unsoundness of the internal structure. In order to monitor the vibrational status of core internal, signals from the ex-core neutron detectors are transformed into frequency domain. By analyzing transformed frequency domain signal, an analyst can acquire the information on the vibrational characteristics of the structures, i.e., vibration frequencies of each component, vibrational level, modes of vibration, and the causes of the abnormal vibration, if any. This study is focused on the development of the automated monitoring system. Several methods are surveyed to define the peaks in power spectrum and fuzzy theory is used to automatic detection of the vibrational peaks. Fuzzy algorithm is adopted to define the modes of vibration using the peak values from fuzzy peak recognition, phase spectrum, and coherence spectrum.

• Korean Journal of Veterinary Service
• /
• v.29 no.3
• /
• pp.317-330
• /
• 2006

This study was conducted to develop the analytical method about simultaneous determination for synthetic antimicrobials in muscle by high performance liquid chromatography - mass selective detector (HPLC- MSD). Solid phase extraction (SPE), matrix solid phase dispersion (MSPD) and liquid-liquid extraction (LLE) have been adapted as pretreatment procedures for HPLC- MSD. Among various solvent tested, methanol was chosen for extraction of synthetic antimicrobials in muscles. For the optimized response, the values of various MS parameters including fragment voltage, drying gas flow, nebulizer pressure, drying gas temperature were verified. The average recovery rates using MSPD and SPE for muscles of bovine and pork were 78.9-127.1% and 78.3-121.7%, respectively. This method was verified the satisfactory performance for fourteen synthetic antimicrobials excepting carbadox in muscle of pork as detection limit of $0.05{\mu}g/g$ on API/ES SIM mode.

• Convergence Security Journal
• /
• v.6 no.4
• /
• pp.75-82
• /
• 2006

Internet worm gives various while we paralyze the network and flow the information out damages. In this paper, I suggest the method to prevent this. This method detect internet worm in PC first. and present the method to do an automatic confrontation. This method detect a traffic foundation network scanning of internet worm which is the feature and accomplish the confrontation. This method stop the process to be infected at the internet worm and prevent that traffic is flowed out to the outside. and This method isolate the execution file to be infected at the internet worm and move at a specific location for organizing at the postmortem so that we could accomplish the investigation about internet worm. Such method is useful to the radiation detection indication and computation of unknown internet worm. therefore, Stable network operation is possible through this method.

• Journal of the Korean Society for Precision Engineering
• /
• v.26 no.11
• /
• pp.55-61
• /
• 2009

The wall thinning defect of nuclear power pipe is mainly occurred by the affect of the flow accelerated corrosion (FAC) of fluid. This type of defect becomes the cause of damage or destruction of in carbon steel pipes. Therefore, it is very important to measure defect which is existed not only on the welding part but also on the whole field of pipe. This study use dual-beam Shearography, which can measure the out-of-plane deformation and the in-plane deformation by using another illuminated laser beam and simple image processing technique. And this study proposes Infrared thermography, which is a two-dimensional non-contact nondestructive evaluation that can detect internal defects from the thermal distribution by the inspection of infrared light radiated from the object surface. In this paper, defect of nuclear power pipe were, measured using dual-beam shearography and infrared thermography, quantitatively evaluated by the analysis of phase map and thermal image pattern.