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Optical Detection of Red Blood Cell Aggregation in a Disposable Microfluidic Channel  

Shin Sehyun (School of Mechanical Engineering, Kyungpook National University)
Jang Ju-Hee (School of Mechanical Engineering, Kyungpook National University)
Park Myung-Soo (School of Mechanical Engineering, Kyungpook National University)
Ku Yunhee (School of Mechanical Engineering, Kyungpook National University)
Suh Jang-Soo (Department of Laboratory Medicine, Kyungpook National University Hospital)
Publication Information
Journal of Mechanical Science and Technology / v.19, no.3, 2005 , pp. 887-893 More about this Journal
Abstract
The aggregability of red blood cells (RBCs) was determined by laser backscattering light analysis in a microfluidic channel. Available techniques for RBC aggregation often adopt a rotational Couette-flow using a bob-and-cup system for disaggregating RBCs, which causes the system to be complex and expensive. A disposable microfluidic channel and vibration generating mechanism were used in the proposed new detection system for RBC aggregation. Prior to measurement, RBC aggregates in a blood sample were completely disaggregated by the application of vibration-induced shear. With the present apparatus, the aggregation indexes of RBCs can be measured easily with small quantities of a blood sample. The measurements with the present aggregometer were compared with those of LORCA and the results showed a strong correlation between them. The aggregability of the defibrinogenated blood RBCs is markedly lower than that of the normal RBCs. The noble feature of this design is the vibration-induced disaggregation mechanism, which can incorporate the disposable element that holds the blood sample.
Keywords
Aggregation; Red Blood Cell; Vibration; Light-Backscattering;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
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