Journal of Mechanical Science and Technology

The Korean Society of Mechanical Engineers (대한기계학회)
권호 표지

Optical Detection of Red Blood Cell Aggregation in a Disposable Microfluidic Channel

  • Shin Sehyun (School of Mechanical Engineering, Kyungpook National University) ;
  • Jang Ju-Hee (School of Mechanical Engineering, Kyungpook National University) ;
  • Park Myung-Soo (School of Mechanical Engineering, Kyungpook National University) ;
  • Ku Yunhee (School of Mechanical Engineering, Kyungpook National University) ;
  • Suh Jang-Soo (Department of Laboratory Medicine, Kyungpook National University Hospital)
  • Published : 2005.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aggregability of red blood cells (RBCs) was determined by laser backscattering light analysis in a microfluidic channel. Available techniques for RBC aggregation often adopt a rotational Couette-flow using a bob-and-cup system for disaggregating RBCs, which causes the system to be complex and expensive. A disposable microfluidic channel and vibration generating mechanism were used in the proposed new detection system for RBC aggregation. Prior to measurement, RBC aggregates in a blood sample were completely disaggregated by the application of vibration-induced shear. With the present apparatus, the aggregation indexes of RBCs can be measured easily with small quantities of a blood sample. The measurements with the present aggregometer were compared with those of LORCA and the results showed a strong correlation between them. The aggregability of the defibrinogenated blood RBCs is markedly lower than that of the normal RBCs. The noble feature of this design is the vibration-induced disaggregation mechanism, which can incorporate the disposable element that holds the blood sample.

Keywords

References

  1. Baskurt, O. K., Meiselman, H. J. and Kayar, E., 1998, Measurement of Red Blood Cell Aggregation in a 'Plate-Plate' Shearing System by Analysis of Light Transmission, Clin. Hemorheol & Microcirc., Vol. 19, pp. 307-314
  2. Chien, S., Feng, S., Vayo, M., Sung, L. A., Usami, S. and Sakalak, R. , 1990, 'The Dynamics of Shear Disaggregation of Red Blood Cells in a Flow Channel,' Biorheology, Vol. 27, pp. 135-147 https://doi.org/10.3233/BIR-1990-27202
  3. Hardeman, M. R., Goedhart, P. T., Dobbe, J. G. G. and Lettinga, K. P., 1994, Laser-Assisted Optical Rotational Cell Analyzer (LORCA) : a New Instrument for Measurement of Various Structural Hemorheological Parameters, Clin. Hemorheol. Vol. 14, pp. 605-618
  4. Hardeman, M. R., Dobbe, J. G. G. and Ince, C., 2001, The Laser-Assisted Optical Rotational Cell Analyzer (LORCA) as Red Blood Cell Aggregometer, Clinical Hemorheology and Microcirculation, Vol. 25, pp 1-11
  5. Rampling, M. W., 1999, In Cardiovascular Flow Modeling and Measurement with Application to Clinical Medicine, Edited by S. G. Sajidai, G. B. Nash and M. W. Rampling (Clarendon Press, Oxford, pp. 183-193
  6. Sakalak, R. and Chien, S., 1983, 'Theoretical Models of Rouleaux Formation and Disaggregation,' Ann. N.Y. Acad. Sci., Vol. 416, pp. 138-148 https://doi.org/10.1111/j.1749-6632.1983.tb35184.x
  7. Schmid-Schonbein, H., Volger, E., Teitel, P., Kiesewetter, H., Dauer, U. and Heilmann, L., 1982, Clin. Hemorheol. Vol. 2, p. 93
  8. Schmid-Schonbein, H. and Wells, R. E., 1971, 'Rheologica Properties and Their Influence Up-on Anomalous Viscosity of Blood,' Rev. Physiol, Vol. 63, pp. 147-219
  9. Schmid-Schonbein, H., 1976, 'Microrheology of Erythrocytes, Blood Viscosity and the Distribution of Blood flow in the Microcirculation,' Int. Rev. Physiol. Cardiovas. Phystol. II, Vol. 9, pp. 1-59
  10. Schmid-Schonbein, H., Wells, R., 1969, Fluid Drop-Like Transition of Erythrocytes Under Shear. Science, Vol. 165 pp. 288-291 https://doi.org/10.1126/science.165.3890.288
  11. Shin, S., Ku, Y. H., Suh, J. S., Moon, S. Y. and Jang, J. Y., 2003a, Characteristics of Blood Flow Resistance Under Transverse Vibration : Red Blood Cell Suspension in Dextran-40, Annals of Biomedical Eng., Vol. 31, pp. 1077-1083 https://doi.org/10.1114/1.1603751
  12. Shin, S., Ku, Y. H., Park, M. S. and Suh, J. S., 2003b, The Effect of Vibration on the Hemorheological Characteristics of Non-Aggregated Blood, KSME Int. J., Vol. 16, pp. 1719-1724
  13. Stoltz, J. F., Singh, M. and Riha, P., 1999, Mierorheological Parameters (Ch. 4) , in: Hemorheology in Practice, pp. 27-52
  14. Zhao, H., Wang, X. and Stolz, J. F., 1999, Comparison of three optical methods to study erythrocyte aggregation, Clin. Hemorheol & Microcirc., Vol. 21 pp. 297-302