• Archives of Pharmacal Research
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• 제17권4호
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• pp.236-239
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• 1994

The suppressive activity of isoflavonoids against lymphocyte proliferation in vitro was examined. Isoflabvonoid derivatives tested were isflavones isolated from Pueraia radix and synthesized 7-O-substituted biochanin A derivatives. The certain isoflavones such as biochanin A and 2-carbethoxybiochainin A were found to possess the suppressive activity against concanavaline A (Con A)-induced lymphocyte proliferation from mouse spleen. Against mixed lymphocyte culture reaction, biochanin A, 2-carbethoxybiochainin A, daidzein, formononetin, genistein and 7-O-isopropylbiochaninl A showed the suppressive activity at $10^{-5}$ M. However, all isoflavones tested did not show the suppressive activity against lymphocyte proliferation induced by B-cell mitogen, lipopolysaccharide (LPS). In general, isoflavones were revealed to be less active than flavones/flavonols.

• Archives of Pharmacal Research
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• 제27권3호
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• pp.278-282
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• 2004

A number of 8-arylflavones have been synthesized as congeners of wogonin and evaluated for their inhibitory activities of $PGE_2$ production. 8-Arylflavones were obtained from commercially available chrysin via two different synthetic pathways. Most 8-arylflavones exhibited much reduced inhibitory activities against COX-2 catalyzed $PGE_2$ production compared to that of wogonin. Functional group replacement at the 8-position of wogonin from methoxy to aryl group caused loss of inhibitory activity. Our present results imply that the functional group at the 8-position of flavones seems to play very important roles for bioactivity.

• Natural Product Sciences
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• 제4권2호
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• pp.53-57
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• 1998

Leaves of Crataegus sinaica Boiss, contain the new C-glycosyl flavone $3"',4”’-di-O-acetyl-2“-O-{\alpha}-rhamnosylvitexin$, together with the hitherto unknown, dihydroflavonol 3-O-xyloside, (2R:3R)-dihydroquercetin-$3-O-{\beta}-xylopyranoside$. The known compounds (+)-catechin, (-)-epicatechin, vicenin-II, $2"-O-{\alpha}-rhamnosylvitexin$, and $4"'-O-acetyl-2"-O-{\alpha}-rhamnosylvitexin$ were also characterized. Structures were established by conventional methods of analysis and confirmed by $^1H-,\;^{13}C-NMR$, and ESI-MS.

• Natural Product Sciences
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• 제19권3호
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• pp.215-220
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• 2013

Three C-glucosyl flavones (1 - 3), one xanthone (4), and four flavanonols (5 - 8) were isolated by various chromatographic methods from the leaves and stems of Desmodium caudatum (Thunb.) DC. Chemical structures of these compounds were elucidated by 1D and 2D NMR and mass spectroscopy. The compounds were identified as swertisin (1), spinosin (2), 7-methyl-apigenin-6-C-${\beta}$-glucopyranosyl-2"-O-${\beta}$-D-xylopyranoside (3), 1,3,5,6-tetrahydroxyxanthone (4), yokovanol (5), aromadendrin (6), 2'-hydroxy yokovanol (7), and 2'-hydroxy neophellamuretin (8). Compounds 2 - 4 were first isolated from D. caudatum, as well as the spectroscopic data for compound 3.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.196-196
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• 1998

Nitric oxide is involved in various physiological processes. Among isoforms of nitric oxide synthase, iNOS is partly responsible for inflammation and septic shock. During our continual search for anti-inflammatory flavonoids, we have found that flavonoids, especially flavones, possessed the inihibitory activity of NO production by iNOS from LPS-activated RAW 264.7 cell. In this study, flavonoids from Scutellaria radix were investigated for their inhibitory activity of nitric oxide production. It was found that wogonin, among tested flavonoids including baicalein, oroxylin A, skullcapflavone II, showed the strongest inhibition of nitric oxide production (IC$\sub$50/ = 17 uM). And this inhibition was, at least partly, due to down-regulation of iNOS enzyme induction, not due to direct inhibition of iNOS enzyme activity.

• Archives of Pharmacal Research
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• 제26권5호
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• pp.345-350
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• 2003

5,7-Dihydroxyflavones and their Ο-methylated flavone analogs were prepared and evaluated their anti-inflammatory activity to decipher the structure-activity relationships. Most of the analogs were achieved from 2,4,6-trihydroxyacetophenone in 4 steps. 5,7-Dihydroxy-4 -methoxyflavone (4c) and 7-hydroxy-4 ,5-dimethoxyflavone(6c) were prepared following a different synthetic pathway. Among the synthetic flavones tested, 5-hydroxy-7-methoxyflavone analogs (3a-3e) showed moderate inhibitory activities of $PGE_2$ production from LPS-induced RAW 264.7 cells.

• 약학회지
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• 제47권6호
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• pp.376-381
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• 2003

2-Arylchromones, also known as flavones, are among the most ubiquitous classes of natural products occurring in the plant kingdom. On the other hand, 2-styrylchromones are relatively scarce in nature and only a few compounds has been isolated from the blue-green algae species. Therefore, new 2-arylethenylchromone derivatives ( 4a∼n, 5a∼f) were synthesized by the aldol condensation of 2-methylchromone ( 3) with several aromatic aldehydes in order to evaluate their cytotoxicities using a MTT assay on three tumor cells. 2-Arylethenylchromone derivative 4a showed the significant cytotoxic activities on KB, HL-60 and P-388 cell lines with $IC_{50}$/ values of 25.2, 63.59 and 49.51 $\mu$M, respectively, indicating that 2-arylethenylchromone skeleton has a potential anti-tumor application.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2001년도 춘계학술대회 발표논문집
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• pp.49-52
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• 2001

뽕잎은 본초강목과 동의보감에 소갈증, 뇌졸중 등에 효과가 있다고 기록되어 있으며 최근의 과학적인 연구결과서도 중국의 전통생약으로 당뇨병을 예방, 치료하며 잎에는 flavones, steroids, triterepenes, 다량무기질 성분이 함유되어 있다. 이외에도 여러 생리활성에 대한 연구가 밝혀지고 있으며 다방면에서의 이용가능성과 기능부여에 대한 기능성소재로서의 이용가치가 높은 것으로 사료되나 아직 식생활에 응용되는 실질적인 연구가 매우 미비한 상태이다. 따라서 본 연구에서는 혈압조절에 효과가 있는 다량무기질(칼슘, 칼륨, 마그네슘)함량이 풍부한 다식을 제조하고자 뽕잎분말을 여러 비율(0-4%)로 첨가하여 관능평가, 일반성분 및 무기질함량 등을 분석한 결과 뽕잎분말을 2%첨가한 다식의 관능평가가 가장 우수하였으며 일반성분과 무기질함량은 뽕잎의 첨가비율이 높을수록 유의적으로 증가되었다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.172-172
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• 2002

This study was carried out to develop the antitoxic compound about cytotoxicity of cadmium on NIH 3T3 fibroblasts. These cells divided into 3 groups: control groups (cadmium only) or MTT50 group (NIH 3T3 fibroblasts, 53.32 ${\mu}$M cadmium) and experimental group (53.32 ${\mu}$M quercitrin and 54.72 ${\mu}$M quercetin). (omitted)

• 약학회지
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• 제50권6호
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• pp.398-402
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• 2006

Flavone was found to inhibit tyrosinase and reduce synthesis of melanin to show skin-lightening effect. With the hope of identifying skin-lightening flavones, we synthesized flavone analogs. Substituted benzoic acids (1) were treated with oxalyl chloride in DMF to yield benzoyl chlorides (2), which were reacted with 2-hydroxyacetophenones (3) to afford 2-benzoyloxyacetophenones (4). These acetophenones (4) were converted into 1,3-diketones (5) with base and then treated with acid to give flavone derivatives (6).