• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.12
• /
• pp.6191-6196
• /
• 2012

Aims and Background: Traditional chemotherapy strategies for human leukemia commonly use drugs based on cytotoxicity to eradicate cancer cells. One predicament is that substantial damage to normal tissues is likely to occur in the course of standard treatments. Obviously, it is urgent to explore therapies that can effectively eliminate malignant cells without affecting normal cells. Our previous studies indicated that ginsenoside $Rg_1$ ($Rg_1$), a major active pharmacological ingredient of ginseng, could delay normal hematopoietic stem cell senescence. However, whether $Rg_1$ can induce cancer cell senescence is still unclear. Methods: In the current study, human leukemia K562 cells were subjected to $Rg_1$ exposure. The optimal drug concentration and duration with K562 cells was obtained by MTT colorimetric test. Effects of $Rg_1$ on cell cycle were analyzed using flow cytometry and by SA-${\beta}$-Gal staining. Colony-forming ability was measured by colony-assay. Telomere lengths were assessed by Southern blotting and expression of senescence-associated proteins P21, P16 and RB by Western blotting. Ultrastructural morphology changes were observed by transmission electron microscopy. Results: K562 cells demonstrated a maximum proliferation inhibition rate with an $Rg_1$ concentration of $20{\mu}\;mol{\cdot}L^{-1}$ for 48h, the cells exhibiting dramatic morphological alterations including an enlarged and flat cellular morphology, larger mitochondria and increased number of lysosomes. Senescence associated-${\beta}$-galactosidase (SA-${\beta}$-Gal) activity was increased. K562 cells also had decreased ability for colony formation, and shortened telomere length as well as reduction of proliferating potential and arrestin $G_2$/M phase after $Rg_1$ interaction. The senescence associated proteins P21, P16 and RB were significantly up-regulated. Conclusion: Ginsenoside $Rg_1$ can induce a state of senescence in human leukemia K562 cells, which is associated with p21-Rb and p16-Rb pathways.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.24 no.3
• /
• pp.259-264
• /
• 2014

This paper is research paper on the MPD(Magnet Position Device) for the outdoor MGV(Magnet /Magnet Gyro Guidance Vehicle). Usually, MGV is used in indoor environment because of a measurement height of the magnet position device. CMPD(Commercial magnet position device) has 30 mm measurement height, so this is suitable structure in indoor environment like to a flat surface. Outdoor environment is an uneven and irregular, So Outdoor MGV must has a suspension. But CMPD is unsuitable for outdoor environment because of a collision with a surface caused by suspension. Thus, measurement height of the outdoor MPD is positively necessary more than 100 mm. So, we suggest the outdoor MPD using analog magnet hall sensor, moving average filter and Characteristic(rate of the magnet hall sensor) function of the localization. Result of the experiments, the proposed Magnet Position Device for the outdoor MGV has localization accuracy 4.31 mm, measurement height 150 mm and width 150 mm and is efficient more than CMPD.

• Journal of Wetlands Research
• /
• v.9 no.2
• /
• pp.21-31
• /
• 2007

Estuarine wetland, where freshwater meets saltwater, is a transitional ecosystem that is valuable ecologically for a variety of reasons, such as feeding and breeding sites for birds, fish, and wildlife. However, research on the spatial distribution and temporal dynamics of estuarine wetlands in Korea is rare. As a fundamental basis for wetland conservation, this study quantified the wetlands in three major estuaries, and evaluated the temporal dynamics of the wetlands since the 1910s. In particular, this study classified the wetland types into mud flat, sand, and emergent-plant types, and estimated the change of each wetland type, using topographic maps produced in the 1910s, 1970s, and 2000s. The wetlands in both the Han and Youngsan River estuaries have declined since the 1910s, but the rate of wetland decline was relatively low before the 1970s, compared to that since the 1970s. The impact of human activities, such as the Youngsan Watershed Comprehensive Development Project and the construction of estuary barrages, has disrupted the estuary cycles and destroyed huge amounts of wetland in the Youngsan estuary. By contrast, estuarine wetlands have been preserved in the small Gahwa estuary, and provide a variety of habitats for plants and wildlife. A special management strategy for wetlands should be established as soon as possible.

• Restorative Dentistry and Endodontics
• /
• v.20 no.1
• /
• pp.97-112
• /
• 1995

The purpose of this study was to evaluate the effect of wetting condition made by drying time on bonding of resin cement to dentin. Freshly extracted bovine teeth were grinded to expose flat dentin surfaces. After the exposed dentin surfaces were treated with pretreatment agents and water rinse, each wetting condition of dentin surfaces was made according to drying times and methods including slight blow bry for I-second by air syringe, blow dry for 20-second by air syringe, and 12-hour dry in desiccator respectively. and then, previously made composite resin specimens were bonded onto each conditioned dentin surface of the specimen using Panavia-21(Kuraray Co.), Bistite(Tokuso Co.), and Choice(use with All bond-2, Bisco Inc.) resin cement according as manufacturer's instruction. Bonded specimens were stored in $37^{\circ}C$ distilled water for 24 hours, then the tensile bond strength was measured, cohesive failure rate was calculated, and fractured dentin surfaces and acrylic rod sides were examined under scanning electron microscope. The result were as follows ; In the group of bonding with Panavia-21 resin cement, higher tensile bond strength was seen in 12-hour dry group than in I-second and 20-second dry group(p<0.01). In the group of bonding with Bistite resin cement, higher tensile bond strength was seen in 1-second dry group than in 20-second and 12-hour dry group(p<0.01). In the group of bonding with Choice resin cement, no significant differences of bond strength under given drying time were seen. Cohesive failure rates derived from the groups of bonding with Panavia-21 and Choice resin cement were increased with the increase of tensile bond strength in each drying time. On SEM examination of fractured surface, adhesive failure mode with fractured resin tags was mostly seen in wet condition with I-second drying time in the group of bonding with Panavia-21 resin cement, mixed failure mode with shortened and fractured resin tag was seen in the group of bonding with Bistite resin cement, and regardless of drying time, and cohesive-adhesive mixed failure mode with fracture of 'Hollow' typed resin tags was mainly seen in the group of bonding with Choice resin cement.

• Journal of the Korean association of regional geographers
• /
• v.11 no.2
• /
• pp.278-290
• /
• 2005

This study deals with the geomorphic, environmental and land use changes by comparative analysis using Landsat TM images of 1988 year and ETM ones of 2002 year, partly together with the new Quick Bird images having 60cm resolution for more detail analysis, focusing on the Oncheon gun(county) in Pyeongnam Province, west coast zone of North Korea. The main analysis methodology is neural network classification, which is more advanced techniques for the classification of land cover and land use, with higher accuracy rate and lower errors. The TM images of 1988 year show, mainly, the on-construction tide embank for the reclamation at Gwangryangman bay and salt farm on the reclaimed tidal flat. But, ETM images of 2002 year present stabilized reclaimed land, salt farm and rice field, recently transformed from salt farm. Especially, new tidal land has been naturally developed on the coastal shallow out of tide embank and salt farm. The results of the study may help to database coastal environmental changes and to support for reasonable and productive land use of North Korea, and to manage and plan unified national land in the near future.

• Journal of the Korean Vacuum Society
• /
• v.11 no.3
• /
• pp.183-188
• /
• 2002

In the present study AI$(CH_3)_3)$films were deposited by the ALD technique using trimethylaluminum(TMA) and ozone to improve the quality of the AI$(CH_3)_3)$ films, since the $OH^-$ radicals existing in the AI$(CH_3)_3)$ films deposited using TMA and $H_2O$ degrade the physical and the dielectric properties of the AI$(CH_3)_3)$ film. The XPS analysis results indicate that the $OH^-$ radical concentration in the AI$(CH_3)_3)$film deposited using $O_3$is lower than that using $H_2O$. The etch rate of the AI$(CH_3)_3)$film deposited using $O_3$is also lower than that using $H_2O$, suggesting that the chemical inertness of the former is better than the latter. The MIS capacitor fabricated with the TiN conductor and the $Al_2$O$_3$dielectrics formed using $O_3$offers lower leakage current, better insulating property and smaller flat band voltage shift $({\Delta}V_{FB})$.

• Journal of the Korean Vacuum Society
• /
• v.12 no.3
• /
• pp.168-173
• /
• 2003

Recently, according to the rapid progress in Flat-panel-display industry, there has been a growing interest in the poly-Si process. Compared with a-Si, poly-Si offers significantly high carrier mobility, so it has many advantages to high response rate in Thin Film Transistors (TFT's). We have investigated a new process for the high temperature Solid Phase Crystallization (SPC) of a-Si films without any damages on glass substrates using thin film heater. because the thin film heater annealing method is a very rapid thermal process, it has very low thermal budget compared to the conventional furnace annealing. therefore it has some characteristics such as selective area crystallization, high temperature annealing using glass substrates. A 500 $\AA$-thick a-Si film was crystallized by the heat transferred from the resistively heated thin film heaters through $SiO_2$ intermediate layer. a 1000 $\AA$-thick $TiSi_2$ thin film confined to have 15 $\textrm{mm}^{-1}$ length and various line width from 200 to 400 $\mu\textrm{m}$ was used as the thin film heater. By this method, we successfully crystallized 500 $\AA$-thick a-Si thin films at a high temperature estimated above $850^{\circ}C$ in a few seconds without any thermal deformation of g1ass substrates. These surprising results were due to the very small thermal budget of the thin film heaters and rapid thermal behavior such as fast heating and cooling. Moreover, we investigated the time dependency of the SPC of a-Si films by observing the crystallization phenomena at every 20 seconds during annealing process. We suggests the individual managements of nucleation and grain growth steps of poly-Si in SPC of a-Si with the precise control of annealing temperature. In conclusion, we show the SPC of a-Si by the thin film heaters and many advantages of the thin film heater annealing over other processes

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.6 no.1_2
• /
• pp.76-80
• /
• 1973

Cyclina sinensis is an edible bivalve inhabiting wide tidal flats which are exposed to the air at ebb tide along the western coast of Korea. Over the period of June to September 1971, some specimens from a tidal flat near Inchon were submerged in sea water with various concentrations of ammonium hydroxide added and careful observations were made on their fertilization, early development, and metamorphosis of the larvae. The highest rate of fertilization was demonstrated by individuals treated with 1/1000 normal solution of ammonium hydroxide and their fertilized eggs followed normal development, i.e., two cell stage 1.5 hours after fertilization, blastular stage after 4 hours, and trochophore stage after 6 hours. Within 24 hrs after fertilization C. sinensis larvae have acquired the form of early straight-hinge veliger with the mean prodissoconch I with the length of $110\mu$. It takes seven days to get the umbo stage with the mean shell length of $190\mu$ and twenty days to get the morphosing stage with the mean shell length of $260\mu$. The larvae were cultured to the metamorphosing stage with the shell length of $270\mu$ in the laboratory condition.

• Journal of Digital Contents Society
• /
• v.12 no.1
• /
• pp.23-32
• /
• 2011

For the past few years, unlike wireless widespread United States and Japan, expensive cost and platform of the mobile web carrier underground and self-centered made achievement of domestic mobile web market sluggish. Nowadays, more and more people pay attention to the mobile web service because of the increased quality of wireless internet, development of mobile device and variety of the flat-rate payment system. However, another big problem is that mobile web is too hard to use. Also, the poor content of mobile web service is an extremely serious problem. In this paper, we put forward a new method for easy to access mobile web service which is use WINC expansion numbers and users can use these numbers to shit their own website. Along with the benefits of purchase WINC number general users are able to make website and control their website at less time and energy.

• Bulletin of the Korean Chemical Society
• /
• v.24 no.11
• /
• pp.1659-1663
• /
• 2003

$Al_2O_3$ thin films were grown on H-terminated Si(001) substrates using dimethylaluminum isopropoxide [DMAl: $(CH_3)_2AlOCH(CH_3)_2$], as a new Al precursor, and water by atomic layer deposition (ALD). The selflimiting ALD process by alternate surface reactions of DMAI and $H_2O$ was confirmed from measured thicknesses of the aluminum oxide films as functions of the DMAI pulse time and the number of DMAI-$H_2O$ cycles. Under optimal reaction conditions, a growth rate of ~1.06 ${\AA}$ per ALD cycle was achieved at the substrate temperature of $150\;^{\circ}C$. From a mass spectrometric study of the DMAI-$D_2O$ ALD process, it was determined that the overall binary reaction for the deposition of $Al_2O_3\;[2\;(CH_3)_2AlOCH(CH_3)_2\;+\;3\;H_2O\;{\rightarrow}\;Al_2O_3\;+\;4\;CH_4\;+\;2\;HOCH(CH_3)_2]$can be separated into the following two half-reactions: where the asterisks designate the surface species. Growth of stoichiometric $Al_2O_3$ thin films with carbon incorporation less than 1.5 atomic % was confirmed by depth profiling Auger electron spectroscopy. Atomic force microscopy images show atomically flat and uniform surfaces. X-ray photoelectron spectroscopy and cross-sectional high resolution transmission electron microscopy of an $Al_2O_3$ film indicate that there is no distinguishable interfacial Si oxide layer except that a very thin layer of aluminum silicate may have been formed between the $Al_2O_3$ film and the Si substrate. C-V measurements of an $Al_2O_3$ film showed capacitance values comparable to previously reported values.