• Bulletin of the Korean Chemical Society
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• v.27 no.5
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• pp.657-662
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• 2006

Identification of accessible sites in targeted RNAs is a major limitation to the effectiveness of antisense oligonucleotides. A class of antisense oligodeoxynucleotides, known as the “10-23” DNA enzyme or DNAzyme, which is a small catalytic DNA, has been shown to efficiently cleave target RNA at purine-pyrimidine junctions in vitro. We have designed a strategy to identify accessible cleavage sites in the target RNA, which is hepatitis C virus nonstructural gene 3 (HCV NS3) RNA that encodes viral helicase and protease, from a pool of random DNAzyme library. A pool of DNAzymes of 58 nucleotides-length that possess randomized annealing arms, catalytic core sequence, and fixed 5'/3'-end flanking sequences was designed and screened for their ability to cleave the target RNA. The screening procedure, which includes binding of DNAzyme pool to the target RNA under inactive condition, selection and amplification of active DNAzymes, incubation of the selected DNAzymes with the target RNA, and target site identification on sequencing gels, identified 16 potential cleavage sites in the target RNA. Corresponding DNAzymes were constructed for the selected target sites and were tested for RNA-cleavage in terms of kinetics and accessibility. These selected DNAzymes were effective in cleaving the target RNA in the presence of $Mg^{2+}$. This strategy can be applicable to identify accessible sites in any target RNA for antisense oligonucleotides-based gene inactivation methods.

• International Journal of Aeronautical and Space Sciences
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• v.16 no.3
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• pp.475-483
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• 2015

We propose a numerical scheme to simulate the time-domain echo signals at tracking radar for a realistic scenario where an EAD (expendable active decoy) and an airborne target are both in dynamic states. On various scenarios where the target takes different maneuvers, the trajectories of the EAD ejected from the target are accurately calculated by solving 6-DOF (Degree-of-Freedom) equations of the motion for the EAD. At each sampling time of the echo signal, the locations of the EAD and the target are assumed to be fixed. Thus, the echo power from the EAD can be simply calculated by using the Friis transmission formula. The returned power from the target can be computed based on the pre-calculated scattering matrix of the target. In this paper, an IPO (iterative physical optics) method is used to construct the scattering matrix database of the target. The sinc function-interpolation formulation (sampling theorem) is applied to compute the scattering at any incidence angle from the database. A simulator is developed based on the proposed scheme to estimate the echo signals, which can consider the movement of the airborne target and EAD, also the scattering of the target and the RF specifications of the EAD. For applications, we consider the detection probability of the target in the presence of the EAD based on Monte Carlo simulation.

• Proceedings of the KSRS Conference
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• v.1
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• pp.79-81
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• 2006

Geostationary ocean satellite, unlike other sun-synchronous polar-orbit satellites, will be able to take a picture of a large region several times a day (almost with every one hour interval). For geostationary satellite, the target region is fixed though the location of sun is changed always. Thus, the ocean signal of a given target point is largely dependent on time. In other words, the ocean signal detected by geostationary satellite sensor must translate to the signal of target when both sun and satellite are located in nadir, using another correction model. This correction is performed with a standardization of signal throughout relative geometric relationship among satellite - sun - target points. One signal value of a selected pixel point of the target region of Geostationary Ocean Colour Imager (GOCI) would be set up as a standard, and the ratio of all remained pixel point can be calculated. This relative ratio called bidirectional factor, the result of modelling of spatiotemporal variation of bidirectional factor is shown.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.43 no.8
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• pp.669-676
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• 2015

In this paper, a guidance law for intercepting maneuvering target with a desired impact angle is proposed. The proposed guidance law is modified from the optimal impact angle control law for a fixed target and given by a biased PN law with the impact angle control term in addition to the conventional PN law. Three different kinds of desired impact angles in the respect of LOS angle, flight path angle, and relative flight path angle to the target are defined. The performance of the proposed guidance law is investigated via numerical simulations for various air-to-air engagement scenarios.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.10a
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• pp.13-16
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• 2004

This paper deals with a one-searcher multi-target search problem where targets with different detection priorities move in Markov processes in each discrete time over a given space search area, and the total number of search time intervals is fixed. A limited search resource is available in each search time interval and an exponential detection function is assumed. The searcher can obtain a target detection award, if detected, which represents the detection priority of target and is non-increasing with time. The objective is to establish the optimal search plan which allocates the search resource effort over the search areas in each time interval in order to maximize the total detection award. In the analysis, the given problem is decomposed into intervalwise individual search problems each being treated as a single stationary target problem for each time interval. An associated iterative procedure is derived to solve a sequence of stationary target problems. The computational results show that the proposed algorithm guarantees optimality.

• Current Optics and Photonics
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• v.7 no.6
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• pp.745-754
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• 2023

In order to test the recognition ability and accuracy of a target imaging simulator under the irradiation of solar stray light in a laboratory environment, it needs to be fixed on a five-axis turntable during a hardware-in-the-loop simulation test, so the optical system of the simulator should have a long exit pupil distance. This article adopts a secondary imaging method to design a projection optical system suitable for thin-film-transistor liquid crystal displays. The exit pupil distance of the entire optical system is 1,000 mm, and the final optimization results in the 400 nm-850 nm band show that the modulation transfer function (MTF) of the optical system is greater than 0.8 at the cutoff frequency of 72 lp/mm, and the distortion of each field of view of the system is less than 0.04%. Combined with the design results of the optical system, TracePro software was used to model the optical system, and the simulation of the target imaging simulator at the magnitude of -1 to +6 Mv was analyzed and verified. The magnitude error is less than 0.2 Mv, and the irradiance uniformity of the exit pupil surface is greater than 90%, which meets the requirements of the target imaging simulator.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.1278-1282
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• 2003

A new interacting multiple model (IMM) method using intelligent input estimation (IIE) is proposed to track a maneuvering target. In the proposed method, the acceleration level for each sub-model is determined by IIE-the estimation of the unknown acceleration input by a fuzzy system using the relation between maneuvering filter residual and non-maneuvering one. The genetic algorithm (GA) is utilized to optimize a fuzzy system for a sub-model within a fixed range of acceleration input. Then, multiple models are composed of these fuzzy systems, which are optimized for different ranges of acceleration input. In computer simulation for an incoming ballistic missile, the tracking performance of the proposed method is compared with those of the input estimation (IE) technique and the adaptive interacting multiple model (AIMM) method.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2003.09b
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• pp.99-102
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• 2003

A new interacting multiple model (IMM) method using genetic algorithm (GA)-based intelligent input estimation(IIE) is proposed to track a maneuvering target. In the proposed method, the acceleration level for each sub-model is determined by IIE-the estimation of the unknown acceleration input by a fuzzy system using the relation between maneuvering filter residual and non-maneuvering one. The GA is utilized to optimize a fuzzy system fur a sub-model within a fixed range of acceleration input. Then, multiple models are composed of these fuzzy systems, which are optimized for different ranges of acceleration input. In computer simulation for an incoming ballistic missile, the tracking performance of the proposed method is compared with those of the input estimation(IE) technique and the adaptive interacting multiple model (AIMM) method.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.571-574
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• 2005

In this paper, we propose an efficient image segmentation technique for large-sized monochrome images using a hybrid approach which combines threshold and region-based techniques. First, an image is partitioned into fixed-size blocks and for each block the representative intensity is determined by averaging pixel intensities within the block. Next, the neighborhood blocks that have similar characteristics with respect to a specific threshold are merged in order to form candidate regions. Finally, those candidate regions are refined to get final target object regions by merging regions considering the spatial locality and certain criteria. We have performed experiments on images selected from various domains and showed that our technique was able to extract target object regions appropriately from most images.

• International Journal of Aeronautical and Space Sciences
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• v.15 no.3
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• pp.258-266
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• 2014

We present a system for the real-time visual relative navigation of a fixed-wing unmanned aerial vehicle in a GPS-denied environment. An extended Kalman filter is used to construct a vision-aided navigation system by fusing the image processing results with barometer and inertial sensor measurements. Using a mean-shift object tracking algorithm, an onboard vision system provides pixel measurements to the navigation filter. The filter is slightly modified to deal with delayed measurements from the vision system. The image processing algorithm and the navigation filter are verified by flight tests. The results show that the proposed aerial system is able to maintain circling around a target without using GPS data.