• Journal of Microbiology and Biotechnology
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• 제28권1호
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• pp.1-11
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• 2018

An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structure-activity relationship in these peptides. To prepare antioxidant peptides better and improve their stability, the design and expression of an antioxidant peptide from Pinctada fucata (named DSAOP) was studied. The peptide contains a common precursor of an expression vector containing an ${\alpha}$-helix tandemly linked according to the BamHI restriction sites. The DNA fragments encoding DSAOP were synthesized and subcloned into the expression vector pET-30a (+), and the peptide was expressed mostly as soluble protein in recombinant Escherichia coli. Meanwhile, the DPPH radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging activity of DSAOP $IC_{50}$ values were $0.136{\pm}0.006$, $0.625{\pm}0.025$, and $0.306{\pm}0.015mg/ml$, respectively, with 2-fold higher DPPH radical scavenging activity compared with chemosynthesized AOP (p < 0.05), as well as higher superoxide radical scavenging activity compared with natural AOP (p < 0.05). This preparation method was at the international advanced level. Furthermore, pilot-scale production results showed that DSAOP was expressed successfully in fermenter cultures, which indicated that the design strategy and expression methods would be useful for obtaining substantial amounts of stable peptides at low costs. These results showed that DSAOP produced with recombinant Escherichia coli could be useful in cosmetic skin care products, health foods, and pharmaceuticals.

• 한국지리정보학회지
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• 제16권3호
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• pp.81-90
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• 2013

본 연구는 GIS를 기반으로 한 미더덕 SFG를 이용하여 미더덕 양식장의 적지를 선정하는데 있다. 미더덕 양식은 2001년 15,084M/T의 생산량을 기록하였으나 2005년 1,412M/T로 생산량이 급격히 감소하였다. 이후 연간생산량은 조금씩 증가하여 2012년 2,484M/T를 나타냈으나 여전히 초기 생산물량에 미치지 못하고 있는 실정이다. SFG는 환경과 생물간의 상호연관성을 나타내고 양식생물의 성장도를 나타낸다. SFG 개념을 이용하여 GIS 기반의 적지선정이 가능하며, 양식장의 지속적인 생산성확보를 위한 어장관리시스템 구축에 활용할 수가 있다. 진동만 미더덕 양식장에 대한 GIS 기반의 미더덕 SFG를 이용한 적지 선정을 하여본 결과 해역 전체의 SFG는 0.054~0.57J/day의 범위를 보였으며, 대상해역과 중첩시킨 미더덕 양식장의 SFG 역시 0.054~0.57J/day로 분포하고 있다. 또한 미더덕 양식장의 SFG와 실제 생산량의 상관성 r=0.786, p<0.05 로 높은 상관성을 보이고 있다. GIS 기반 양식장의 서식지적합도 산정을 통한 어장관리시스템 구축은 양식장의 적지선정과 양식장의 지속가능한 생산량을 확보할 수 있으며, 환경의 변동과 양식생물성장의 인과관계를 객관화 할 수 있는 체계를 도입함으로서, 지속적인 어장관리를 위한 의사결정방안을 도출 할 수 있다.

• 수산해양기술연구
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• 제54권2호
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• pp.124-137
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• 2018

Due to the decrease in coastal productivity and deterioration in the quality of ecosystem which result from the excessive overfishing of fisheries resources and the environmental pollution, fisheries resources in the Korean waters hit the dangerous level in respect of quantity and quality. In order to manage sustainable and effective fisheries resources, it is necessary to suggest the potential yield (PY) for clarifying available fisheries resources in the Korean waters. So far, however, there have been few studies on the estimation methods for PY in Korea. In addition, there have been no studies on the comparative analysis of the estimation methods and the substantial estimation methods for PY targeted for large marine ecosystem (LME) For the reasonable management of fisheries resources, it is necessary to conduct a comprehensive study on the estimation methods for the PY which combines population dynamics and ecosystem dynamics. To reflect the research need, this study conducts a comparative analysis of estimation methods for the PY in the Korean waters of the East Sea to understand the advantages and disadvantages of each method, and suggests the estimation method which considered both population dynamics and ecosystem dynamics to supplement shortcomings of each method. In this study, the maximum entropy (ME) model of the holistic production method (HPM) is considered to be the most reasonable estimation method due to the high reliability of the estimated parameters. The results of this study are expected to be used as significant basic data to provide indicators and reference points for sustainable and reasonable management of fisheries resources.

• 한국식품위생안전성학회지
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• 제35권5호
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• pp.447-451
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• 2020

본 연구에서는 2018년 충남지역에서 생산, 출하되는 수산물에 대해 잔류동물용의약품 실태를 조사하였다. 수산물 115건을 대상으로 식품공전 수산물 중 동물용의약품 동시 다성분 시험법으로 분석이 가능한 29종의 잔류동물용의약품을 분석하였다. 115건의 수산물 중 9건에서 6종의 잔류동물용의약품이 검출되었으며, 검출률은 7.8%이었다. 검출된 잔류동물용의약품은 옥소린산, 엔로플록사신이 가장 많이 검출되었으며 시프로플록사신, 설파디아진, 플루메퀸, 옥시테트라사이클린이 각각 검출되었다. 미꾸라지 검체 1건에서 엔로플록사신이 잔류기준을 초과하였으나 전체적으로 안전한 수준으로 평가되었다. 그렇지만 잔류동물용의약품 검출률이 높아지는 추세이고 종류 또한 다양해지고 있어 지속적인 모니터링이 필요하다.

• 스마트미디어저널
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• 제12권11호
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• pp.134-144
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• 2023

Nowadays, Deep Learning (DL) technology is being used in several government departments. South Korea imports a lot of seafood. If the demand for fishery products is not accurately predicted, then there will be a shortage of fishery products and the price of the fishery product may rise sharply. So, South Korea's Ministry of Ocean and Fisheries is attempting to accurately predict seafood imports using deep learning. This paper introduces the solution for the fish import prediction in South Korea using the Long Short-Term Memory (LSTM) method. It was found that there was a huge gap between the sum of consumption and export against the sum of production especially in the case of two species that are Hairtail and Pollock. An import prediction is suggested in this research to fill the gap with some advanced Deep Learning methods. This research focuses on import prediction using Machine Learning (ML) and Deep Learning methods to predict the import amount more precisely. For the prediction, two Deep Learning methods were chosen which are Artificial Neural Network (ANN) and Long Short-Term Memory (LSTM). Moreover, the Machine Learning method was also selected for the comparison between the DL and ML. Root Mean Square Error (RMSE) was selected for the error measurement which shows the difference between the predicted and actual values. The results obtained were compared with the average RMSE scores and in terms of percentage. It was found that the LSTM has the lowest RMSE score which showed the prediction with higher accuracy. Meanwhile, ML's RMSE score was higher which shows lower accuracy in prediction. Moreover, Google Trend Search data was used as a new feature to find its impact on prediction outcomes. It was found that it had a positive impact on results as the RMSE values were lowered, increasing the accuracy of the prediction.

• 한국식생활문화학회지
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• 제19권3호
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• pp.273-284
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• 2004

This study is on the changing pattern of fish-and-shellfish uses during the last two thirds of Chosun period on the premise that they are influenced by development of fishing technology. With a literary approach, this paper researches how fish-and-shellfish production and consumption pattern changed, especially in relation to technological development of fisheries. First, we examine into the changes of fishery production. And next, we analyze the kind and cooking method of fish-and-shellfish in [Eum-sik-di-mi-bang](1670) and [Gyu-hap-chong-seo](1815). The analysis of two books revealed that there were significant differences in fish-and-shellfish uses. Because the two books were written with a gap of 145 years and the development of fishing technology and remarkable changes of fishery production affected on the fish-and-shellfish uses. Due to primitive fishing tools and skills, fresh-water and reverse-river-fish and shellfish had been caught in substantial amount until the middle ages of Chosun period. As a result, the availability of seafood were limited extremely even in the upper classes. These situations are evidenced by the analysis of [Eum-sik-di-mi-bang]. Only 12 kinds of fish-and-shellfish are described in [Eum-sik-di-mi-bang]. Most of the sesfoods is mollusc which is easy to catch. As for the salt-water fish, dried cod and dried herring were mentioned. Mullet, the reverse-water-fish, is used most frequently. Only one kind of 'Hoe', which needs extreme freshness, is described. This means that the use of fresh fish-and-shellfish was very limited to some kinds which could be caught near the village. As the netting fishery began to be developed in the 18th century, the production of some salt-water fishes, such as anchovy, shrimp, yellow corvina, pollack, and herring, had increased remarkably to make marine resources more available. Small fish, such as anchovy and shrimp were preserved as 'Jeot-gal' and sold nationwide. Therefore, 'Jeot-gal' and seafood could be used in Kimchi around this time and had a deep influence on the change of Kimchi in taste and nutrition. In [Gyu-hap-chong-seo], 33 kinds of fish-and-shellfish are described. Including cod and herring, 17 kinds of sea water fish and mollusc are mentioned. Some of these are consumed in fresh state, neither as dried nor as salted. Because the merchants promoted the transport of seafoods to other regions according to the growth of commercial economy. As a result the diet of the people could be enriched by the various seafoods.

• 한국지리정보학회지
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• 제24권1호
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• pp.112-125
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• 2021

해양공간계획은 해양을 체계적이고 합리적으로 관리하기 위해 9가지 용도구역으로 지정한다. 그 중 하나가 어업활동의 보호와 육성을 비롯한 수산물의 지속 가능한 생산을 위해 필요한 어업활동 보호구역이다. 본 연구는 V-Pass 자료를 활용하여 어업활동 지도를 제작하고 어업활동 밀집 공간을 도출함으로써 어업활동보호구역 지정에 필요한 요소 중 하나인 어업활동 공간을 정량적으로 파악하고자 한다. 이를 위해 V-Pass 자료를 정적 정보와 동적 정보가 결합된 데이터셋 구축, 어선 속도 계산, 어업활동 지점 추출, 비어업활동 공간 내의 자료 제거와 같은 전처리를 수행하였다. 최종적으로 선별된 V-Pass 점 자료를 이용하여 커널밀도추정으로 어업활동 지도를 제작하고 어업활동이 밀집된 공간을 분석하였다. 또한 어선의 업종과 계절에 따라 어업활동의 공간분포는 차이가 있음을 확인하였다. 본 연구를 통해 수행한 대용량 V-Pass 자료의 전처리 기법과 어업활동의 공간밀도 분석 방법은 향후 어업활동에 대한 공간특성평가 연구에 기여할 것으로 기대된다.

• 수산경영론집
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• 제36권3호
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• pp.89-117
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• 2005

Currently, the tax - exempted vessel fuel is provided for commercial fishing in order to increase the competitive power of fishery production thorough the National Federation of Fisheries Cooperatives. The National Federation of Fisheries Cooperatives should predict the exact amount of fuel consumption for fishing every year to request the fuel from the government. Unfortunately, there is no sophisticated model to predict the tax - exempted vessel fuel consumption. In 2003, the consumption of the tax- exempted vessel fuel was only $25.1\%$ of the estimation amount by the National Federation of Fisheries Cooperatives. This causes an inefficiency in the petroleum management. Moreover, we need some data such as the annual average fishing hours, fishing days and fishing behavior to adopt a new policy regarding fishing. Up to now, the data have been obtained by survey with response in the fishery field. In the most case, we have a small number of data because we spend so much time and money consuming for collecting fishing data. As a result, the level of confidence of the data is associated with the sample size and normally low. In order to achieve more accurate data, we need to develope an efficient method for collecting fishing data. In this research, we proposed a new method to predict the tax- exempted vessel fuel consumption more exactly. The prediction results from the proposed method has been compared with the results from the current method. According to the results in this research, the method proposed here produced much better accuracy than the current method. In addition, we also proposed in the paper for collecting fishing data of the annual average fishing hours using the tax - exempted vessel fuel consumption and the gasoline consumption of vessel engine. The fishing data obtained by using the method proposed in this research could be much more efficient and accurate because it doesn't need to estimate from survey sample data.

• 수산해양기술연구
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• 제59권2호
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• pp.110-116
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• 2023

In this study, samples of sea anchor canopy cloth mainly used in Korean jigging fishing vessels were collected and tested for performance evaluation. The canopy cloth of sea anchor is a basic element of form composition that is known to have the greatest influence on anchor performance. In order to evaluate the performance of sea anchor canopy cloth, five types of samples were tested for new metric count, tensile strength, water vapour transmission rate and drying speed according to the national standard (KS), and some correlations were identified. As a result of the test, the new metric count of cloths was 335.5-443.4 denier in warp and 217-447.6 denier in weft, and the minimum tensile strength was 860 N in warp direction and 430 N in weft direction. The apparent number and tensile strength of cloth were proportional, the water vapour transmission rate of the sample was 206.8 g/m²h, and the drying speed was 90-100 min. This study partially confirmed the performance evaluation based on speculation by the standard test method, and further research is needed on the clear relationship between the research results and the performance of the sea anchor.

• 수산경영론집
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• 제54권2호
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• pp.1-14
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• 2023

The sustainability of fishing villages is threatened by manpower shortages due to population aging and poor settlement conditions. In the reality of poor fishing villages, the importance of women's labor is more emphasized than that of the past because female workers are key workers in fishing labor, processing and sales of marine products. However, policy support for female fishermen is not sufficient. The reason for this is that policy makers did not properly recognize the labor value of female fishermen. In fact, fishing villages have emphasized the importance of female workforce as a slogan, but there has been no attempt to estimate the labor value. There was not even a review of the methodology for estimating the value of labor that was attempted in similar fields. As a result, the policy importance of female fishermen was underestimated for there was no attempt to evaluate their value even though women had been continuously participating in the fishery from the past. Female fishermen's labor is under the dual labor structure of housework and fishing labor. Therefore, in this study, housework and fishing labor were estimated separately and the total labor value was calculated. The basic data necessary for estimating the labor value of female fishermen were obtained through a survey. The method of estimating the labor value of female fishermen was used in combination with the present income method and the total replacement cost method. As a result of the study, the total labor value of female fishermen was about 4.4 trillion won, which is about half of the total fishery production of 9 trillion won in Korea.