• Journal of Biosystems Engineering
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• v.37 no.5
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• pp.302-313
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• 2012

Purpose: The biogas productivity potential of fish waste (FW) was evaluated. Methods: Batch trials were carried out in 1.3 L glass digesters kept in a temperature controlled chambers at $36.5^{\circ}C$. The first order kinetic model and the modified Gompertz model were evaluated for biogas production. The Chen and Hashimoto model was used to determine the critical hydraulic retention time (HRT $_{Critical}$) for FW under mesophilic conditions. The feasibility of co-digestion of FW with animal manure was studied. Results: The biogas and methane potential of FW was found to be 757 and 554 mL/g VS, respectively. The methane content in the biogas produced from FW was found to be 73% and VS removal was found to be 77%. There was smaller difference between measured and predicted biogas production when using the modified Gompertz model (16.5%) than using first order kinetic model (31%). The time period for 80%-90% of biogas production ($T_{80-90}$) from FW was calculated to be 50.3-53.5 days. Similarly, the HRT $_{Critical}$ for FW was calculated to be 13 days under mesophilic conditions. The methane production from swine manure (SM) and cow manure (CM) digesters could be enhanced by 13%-115% and 17%-152% by mixing 10%-90% of FW with SM and CM, respectively. Conclusions: The FW was found to be highly potential substrate for anaerobic digestion for biogas production. The modified Gompertz model could be more appropriate in describing anaerobic digestion process of FW. It could be promising for co-digestion of FW with animal manure.

• Journal of Life Science
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• v.11 no.1
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• pp.76-82
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• 2001

Fifteen species of microorganisms were isolate form the intestines of fishes, fish feed, and ferment. Eleven microorganisms except HY4, HY8, HY12, and HY13 were Gram-positive, and HY1, HY2, HY3, HY5, HY6, and HY7 produced lactic acid. The species of HY1, HY2, HY3, HY4, HY5, HY6, HY13, and HY14 showed some growth in the medium containing 1% of NaCl. Except HY6, HY7, HY8, HY12 and HY5, 10 isolates had proteolytic activity, whereas only HY13 and HY14 had lipase activity. From all the results four isolates (HY3, HY4, HY13 and HY14) were chosen for the degradation of fish wastes. There was no mutual inhibition among the microorganisms, and the optimum temperature and pH for the growth of the mixed culture were found to be 3 2$\^{C}$ and 7, respectively. Under the optimum growth conditions the maximum optical density and the maximum specific growth rate were estimated to be 2.35 and {TEX}$0.46h^{-1}${/TEX}, respectively. Major microorganisms in the mixed culture at the log-phase were HY3 and HY4, which occupied 70%. The degrading efficiency of fish waste by the mixed microorganisms was 2.3 times higher, compared to control. The total amount of free amino acids in the degraded products from fish wastes was 39g/100g protein and little odor was produced by the mixed microorganisms after 48 hours.

• Journal of Life Science
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• v.19 no.2
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• pp.277-283
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• 2009

Biogenic amines are generally formed through the decarboxylation of specific free amino acids by exogenous decarboxylases released by microbial species associated with the fish products and fermented feeds. This study was conducted to investigate the properties of e tuna waste regarding the control of degradation of biogenic amines (histamine, tyramine, tryptamine, putrescine, and cadaverine) that might be related with the anti-nutritional factor of the tuna waste that is used for manufacturing domestic fish meal. The values of pH and the salt content were 6.51, 3.35% in tuna waste and 5.58 and 5.83% in tuna fish meal, respectively. The strains and dominant bacteria tested in the tuna waste sample were 9.20, 9.29, 5.67, 7.82 and 7.58 log CFU/g of total bacteria, aerobic plate count (APC), total coliform (TC), Lactobacillus spp. and Bacillus spp., respectively. The main histamine forming-bacteria (HFB) in tuna waste were detected by silica gel thin-layer chromatography (TLC) and 7 histamine-forming bacterial species were isolated among microbes grown in selective medium. The histamine concentration was determined by detection of fluorescence of ο-phthaldialdehyde (OPA) derivatives using HPLC and the date were used to reconfirm the identities of the amine-producing bacteria. The 15 histamine- forming bacteria strains grown in trypicase soy broth (TSB) supplemented with 1% L-histidine (TSBH) were identified as Lactococcus(L.) lactis subsp. lactis, Klebsiella pneummonlae, L. garvieae 36, Vibrio olivaceus, Hafnia alvei and L. garvieae which were main dominant amine - producing strains, and Morganella morganii identified by 16S ribosomal RNA (rRNA) sequencing with PCR amplification. A Phylogenetic tree generated from the 16S rRNA sequencing data showed different phyletic lines that could be readily classified as biogenic amine forming gram-positive and negative bacteria.

• Fisheries and Aquatic Sciences
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• v.27 no.2
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• pp.87-99
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• 2024

Global climate change, followed by an increase in anthropogenic activities in aquatic ecosystems, and species invasions, has resulted in a decline in aquatic organism biodiversity. The Batanghari River, Sumatra's longest river, is polluted by mercury-containing illegal gold mining waste (PETI), industrial pollution, and domestic waste. Several studies have provided evidence suggesting a decline in fish biodiversity within the Batanghari River. However, a comprehensive evaluation of the present status of biodiversity in this river is currently lacking. The species under investigation were identified through various molecular-based identification methods, as well as morphological identification, which involved the use of neighbor-joining (NJ) trees. All collected specimens were initially identified using morphological techniques and subsequently confirmed with molecular barcoding analysis. Morphological and DNA barcoding identification categorized all specimens (1,692) into 36 species, 30 genera and 16 families, representing five orders. A total of 36 DNA barcodes were generated from 30 genera using a 650-bp-long fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene. Based on the Kimura two-parameter model (K2P), The minimum and maximum genetic divergences based on K2P distance were 0.003 and 0.331, respectively, and the average genetic divergence within genera, families, and orders was 0.05, 0.12, 0.16 respectively. In addition, the average interspecific distance was approximately 2.17 times higher than the mean intraspecific distance. Our results showed that the COI barcode enabled accurate fish species identification in the Batanghari River. Furthermore, the present work will establish a comprehensive DNA barcode library for freshwater fishes along Batanghari River and be significantly useful in future efforts to monitor, conserve, and manage fisheries in Indonesia.

• KSBB Journal
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• v.20 no.6
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• pp.438-442
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• 2005

Batch experiments were performed to investigate the effects of volumetric mixing ratio(v/v) of two substrates, food wastes(FW) and waste activated sludge(WAS). In batch experiments, optimum mixing ratio for hydrogen production was found at $10{\sim}20$ v/v % addition of WAS. CSTR(Continuous Stirred tank reactor) was operated to investigate the hydrogen productivity and the microbial community under various HRTs and volumetric mixing ratio(v/v) of two substrates. The maximum yield of specific hydrogen production, 140 mL/g VSS, was found at HRT of 2 day and the volumetric mixing ratio of 20:80(WAS:FW). The spatial distribution of hydrogen producing bacteria was observed in anaerobic fermentative reactor using fluorescent in situ hybridization(FISH) method.

• Journal of the Korean Institute of Landscape Architecture
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• v.23 no.2
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• pp.167-181
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• 1995

An Ecocomplex is proposed for ecological design of the estuarine environment of Han River, which is designed upon an alternative mamagement concept of estuarine environment. The concept reveals interrelationships among estuary, delta region and urban inland with inputs/outputs and feedbacks among them. The Ecocomplex emphasizes an integration of wastewater treatment with aquaculture, agriculture and recreation, and carries out ecological treatment, recycling, and harvest processes. A module of wastewater treatment pond system is employed in the Ecocomplex, which treats a flow of 3,786 ㎥/day and is composed of a four-facultative-pond series. Treatment ponds stabilize wastewater discharged from the urban area, and concurrently produce algae for commercial or recreational fish farming. Effluent from treatment and fish ponds is reused for agricultural production. Through the waste-algae-fish-vegetable-recreation processes, wastewater from the urban settlement is recycled back to the urban ecosystem. This resource-conserving design approach can maintain a sustainable urban ecosystem, managing an estuarine environment more naturally, healthly, and economically.

• Korean Journal of Microbiology
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• v.37 no.3
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• pp.204-208
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• 2001

A technique for detection of Bacillus in soil and waste water treatment system was developed. Mixed col- tured solutions of 22 Bacillus strains were applied for selection of probe and pretreatment method for FISHAmong the three probes known as useful tool for FISH method,S-G-Bacill-0597-a-A-22 was best for detec-tion of Bacillus. Ninety five percent of DAPl count was observed with FISH method with S-G-Bacill-0597-a-A-22 probe. For increasing the permeability to Bacillus cell walls, pretreatment with lysozyme was betterthan that with lysozyme and SDS/DTT (dithiothreitol). Bacillus spore was not detected with FISH. So, Bacil-lus detection in ecosystem requires FISH with pretreatment of lysozyme and spore staining.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.95-98
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• 2013

The experiment was conducted to determine the value of black soldier fly (Hermetia illucens) larvae and pupae as feed supplements for fresh-water fish (Carassius auratus). Black soldier fly larvae grown on food waste were fed to fresh-water fish, live larvae alone, dried larvae, dried pupae and combination with commercial fish diet. A 16-week feed trial was conducted to determine whether black soldier fly larvae could be used as a feed supplement in fresh-water fish. The growth performance indicated that fish fed the live larvae ranked consistently higher, and in combination with commercial fish diet and dried larvae and pupae were next higher. The finding of this study suggest that black soldier fly larvae and pupae may be valuable feedstuff in commercial fish production.

• Fisheries and Aquatic Sciences
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• v.5 no.2
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• pp.136-140
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• 2002

Every year fish skin, bone and intestines are discarded as processing waste material. The use of fish processing waste material is more economical and environmental-friendly. The leather-like material was produced using shark intestine. Physical charactistics such as tensile strength, elongation, tongue tearing strength, and bursting strength of the leather-like material were measured, and compared with those of a commercial leather product. The values of tensile strength, elongation, tongue tearing strength, and bursting strength of the leather-like material were $3.3kg/mm^2$, $53\%$, 13.0kg/mm and $18kg/cm^2$, respectively. Elongation $(l09\%)$ of the leather-like material coated with lacquer was higher than that of a commercial leather material, and the other factors were similar. The tensile strength and tongue tearing strength of the leather-like material was higher than those of shoes leather, but bursting strength was lower. These results suggested a potential value to use the leather-like material from shark intestines as a substitute for commercial leathers.

• Microbiology and Biotechnology Letters
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• v.48 no.3
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• pp.358-365
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• 2020

An organic solvent- and bleach-stable protease-producing strain was isolated from a polluted river water sample and identified as Aeromonas veronii OB3 on the basis of biochemical properties (API 20E) and 16S rRNA sequence analysis. The strain was found to hyper-produce alkaline protease when cultivated on fish waste powder-based medium (HVSP, 4080 U/ml). The biochemical properties and compatibility of OB3 with several detergents and additives were studied. Maximum activity was observed at pH 9.0 and 60℃. The crude protease displayed outstanding stability to the investigated surfactants and oxidants, such as Tween 80, Triton X-100, and H₂O₂, and almost 36% residual activity when incubated with 1% SDS. Remarkably, the enzyme demonstrated considerable compatibility with commercial detergents, retaining more than 100% of its activity with Ariel and Tide (1 h, 40℃). Moreover, washing performance of Tide significantly improved by the supplementation of small amounts of OB3 crude protease. These properties suggest the potential use of this alkaline protease as a bio-additive in the detergent industry and other biotechnological processes such as peptide synthesis.