• 한국식품과학회지
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• 제20권6호
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• pp.862-867
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• 1988

한류성어류(연어, 명태)와 난류성어류(상어)의 열변성과 pH의 상관성에 있어서는 서식하는 환경온도에 관계없이 어류의 근원섬유단백질의 ATPase는 pH $6.5{\sim}8.5$ 범위에서 모두 온도에 의존하는 변성을 나타내었다. 한편 열안정성에 대한 열역학량중 D value와 변성속도 Kd value에 있어서는 상어 >연어 >명태 순으로 확실한 차이를 나타내어 어류 근원섬유단백질의 열안정성은 서식하는 어류의 환경온도와 매우 밀접한 관계가 있는것으로 나타났으며, 동물의 종류에 따라서도 차이를 나타내었다.

• 한국어병학회지
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• 제22권3호
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• pp.219-228
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• 2009

The genomic and subgenomic RNAs of fish nodavirus encode the four proteins, protein A, capsid protein, non-structural protein B1 and B2. In this study, we describe the immune response of olive flounder Paralichthys olivaceus immunized with live fish nodavirus or recombinant capsid protein, non-structural protein B1 and B2 expressed in E. coli. Nodavirus-infected flounder produced antibodies to capsid protein, B1 and B2 and nodavirus-neutralizing activities were detected in the serum of the nodavirus-infected flounder. The flounder were immunized against the three recombinant proteins of fish nodavirus and the sera from these immunized fishes were assayed for nodavirus-specific antibody by ELISA and a neutralization test. In the immunized flounder, all three recombinant proteins induced the production of similar levels of antibody, but only the antibody to capsid protein significantly neutralized nodavirus. These results indicate that all three nodaviral proteins are immunogenic in flounder, but only the capsid protein can induce neutralizing antibody against nodavirus.

• 한국식품영양과학회지
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• 제30권4호
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• pp.574-578
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• 2001

담수어인 향어와 해수어인 방어의 white muscle과 red muscle로 부터 근원섬유단백질을 조제하여 생육 환경조건이 다른 어류의 근원섬유단백질간에는 어떠한 차이가 있는지에 대하여 알고자 myofibrillar protein ATPase 활성에 대한 온도의 존성과 열안정성을 실험하였다. 향어와 방어 근원섬유단백질의 기질친화도와 $V_{max}$ 값에 있어서는 향어가 방어에 비해 높은 값을 보였고, 활성에 대한 반응온도 및 반응시간에 따른 영향에 있어서는 myofibril의 경우 red muscle이 white muscle에 비해 낮은 활성을 나타냈으며, actomyosin의 경우에 있어서는 향어가 4$0^{\circ}C$에서도 활성증가를 나타낸 반면에 방어는 45$^{\circ}C$, 5$0^{\circ}C$와 같이 반응시간에 따른 활성증가를 나타내지 않았다. 그리고 일반적으로 향어가 방어보다 그리고 white muscle이 red muscle에 비해 높은 반응생성물을 나타내었다. 열안정성에 대한 열역학량(D value, Z value, )은 muscle 종류간 그리고 환경이 다른 어종간에 차이를 보였다. 향어와 방어 둘다 white muscle이 red muscle에 비해 안정하였으며, 향어 myofibrillar protein이 방어 myofibrillar protein보다 열에 안정한 값을 보여 환경조건이 열안정성에 차이를 줄 수 있는 것으로 나타났다.

• 한국동물학회지
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• 제39권2호
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• pp.123-131
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• 1996

In this study, we examined the expression of heat shock proteins (HSPs) in fish cell line CHSE-2lnl and human HeLa cells exposed to heat shock. In fish CHSE-214 cells HSP70 was the major polvpeptide induced by an elevated temperature or an amino acid analog, while in HeLa cells HSP90 as well as HSP70 were prominently enhanced in response to these stresses. Pretreatment of actinomvcin D prior to heat shock completely inhibited the induction of fish HSP70, indicating the transcriptional regulation of fish HSP70 gene expression. In HeLa and CHSE-214 cells either recovering from heat shock or experiencing prolonged heat shock, attenuation in the HSP90 a'nd HSP70 induction occurred but both induction and repression of HSP70 synthesis appear 19 precede those of HSP90. Moreover, attenuation did not occur in the syntheses of 40 kDa and 42 kOto proteins which were only induced in CHSE-214 cells. The enhanced syntheses of these he proteins continued as long as CHSE-214 cells were Siven heat shock. These results suggest that down-regulation of HSP syntheses during prolonged heat shock may be controlled by several different. as vet undefined, mechanisms.

• Fisheries and Aquatic Sciences
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• 제18권2호
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• pp.137-142
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• 2015

Antifreeze proteins and glycoproteins [AF(G)Ps] constitute a group of proteins that lower the freezing but not the melting points of aqueous solutions, enabling polar and north-temperate fish to survive in ice-laden environments. However, little is known about antifreeze activity in temperate fish; such work would extend our knowledge on the functions and evolution of AF(G)Ps. In the present study, we screened for antifreeze activity in temperate fish caught off the coast of Jumunjin ($37.89^{\circ}N$), Gangneung, Korea. Thermal hysteresis (TH) and the ability to inhibit ice recrystallization (IR) in blood, liver, and muscle samples from nine fish were examined to assess antifreeze activity. As the East Sea off the coast of Jumunjin is ice-free year round, we thought it most unlikely that the fish would express antifreeze proteins. Surprisingly, the blood of Pleurogrammus azonus and three types of tissue from Gymnocanthus herzensteini, Zoarces gilli and Kareius bicoloratus exhibited measurable TH values together with the ability to trigger characteristic morphological changes in ice crystals. Blood samples from the three species also evidenced ice recrystallization (IR) inhibition. This implies that AF(G)Ps or other antifreeze-like substances are present in temperate fish even under nonfreezing conditions. These results contribute to our understanding of the functions and origins of antifreeze activity in fish.

• 한국어병학회지
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• 제22권2호
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• pp.173-177
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• 2009

Lymphocystis disease virus (LCDV) was detected from olive flounder Paralichthys olivaceus, painted glass fish Chanda baculis, gourami Trichogaster leeri and rockfish Sebastes schlegeli, and proteins of the viruses were compared. The major capsid protein (MCP) gene-specific primer sets successfully amplified approximately 1300 bp nucleotides from the olive flounder and 600 bp nucleotides from painted glass fish, gourami and rockfish isolates, respectively. In western blotting analysis using anti-LCDV mouse polyclonal serum, major antigenic proteins had 21, 26, 45, 50, 80, 110 and 120 kDa in olive flounder, 26, 47 and 80 kDa in painted glass fish, 26, 46, 80 and 92 kDa in gourami, 26, 44, 49, 80 and 105 in rockfish, respectively. All the marine and freshwater isolates showed only common antigens of approximately 26 kDa and 80 kDa. These results suggest that antigenic protein profiles of LCDVs may vary depending upon fish species.

• 한국식품과학회지
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• 제20권3호
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• pp.371-379
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• 1988

한류성 어류(연어, 명태)와 난류성 어류(상어, 조기)의 근원섬유 단백질의의 생화학적인 특성을 규명하기 위하여 각각으로부터 actomyosin과 myofibril을 조제하여 생물활성 및 SDS-polyacrylamide gel 전기영동상을 조사하였으며, 또한 어류의 환경온도에 대한 적응성을 알아보기 위하여 생물활성의 온도 의존성을 조사하였다. 각 어류의 myofibril의 SDS-polyacrylamide gel 전기영동상으로부터 SDS-PAGE pattern은 어종간에 차이를 보였으며 또한 myofibril과 actomyosin의 생물활성과 activity-ph curve도 어종간에 차이를 나타내었다. 한류성 어류인 명태의 근원섬유 단백질의 ATPase의 활성은 환경온도$(5^{\circ}C-10^{\circ}C)$ 부근에서 상어보다 높은 활성을 나타내었다.

• 한국어병학회지
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• 제28권2호
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• pp.93-98
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• 2015

Full-length recombinant VP2 and VP3 proteins of aquabirnavirus isolated from olive flounder were expressed successfully in E. coli expression system. After rats were immunized with these proteins, antisera were used for in vitro and in vivo neutralization test. In in vitro test, VP2 antibody titers were higher than that of VP3. In in vivo assays, fish challenged with aquabirnavirus neutralized with VP2 antibody survived longer than other fish.

• 한국어병학회지
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• 제33권2호
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• pp.163-169
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• 2020

Snakehead rhabdovirus (SHRV) is different from other fish novirhabdoviruses such as viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and hirame rhabdovirus (HIRRV) in that it replicates at high temperatures. Therefore, the delivery of foreign proteins to fish living at high water temperature would be possible by using recombinant SHRVs. In the present study, to evaluate the possible use of SHRV as a vehicle for foreign proteins delivery, we generated a recombinant SHRV that contains an enhanced-GFP (eGFP) gene between nucleoprotein (N) and phosphoprotein (P) genes (rSHRV-A-eGFP), and another recombinant SHRV expressing two heterologous genes by inserting an eGFP gene between N and P genes, and mCherry gene between P and M genes (rSHRV-AeGFP-BmCherry). Epithelioma papulosum cyprini (EPC) cells infected with the recombinant SHRVs showed strong fluorescence(s), suggesting the possible availability of recombinant SHRVs for the development of combined vaccines by expressing multiple foreign antigens.

• 한국수산과학회지
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• 제53권3호
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• pp.464-470
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• 2020

This study was conducted to replace fish meal (FM) with three plant proteins (soybean meal, soy protein concentrate, and wheat gluten) in diets for growing olive flounder Paralichthys olivaceus. The control diet was formulated to contain 65% sardine FM and four other replacement diets were formulated to replace FM with the plant proteins by 25, 30, 35 and 40% (designated FM25, FM30, FM35 and FM40, respectively). The replacement diets were added with three essential amino acids (lysine, methionine and threonine) to meet their requirements for the fish. Olive flounder (initial average weight, 96.8±0.2 g) were randomly distributed into 20 tanks (425 L each) at a density of 25 fish per tank. Four replicate groups of fish were fed one of the diets two times daily for 15 weeks. At the end of the feeding trial, no significant differences were found among all the fish groups in growth performance, feed utilization, nonspecific immune responses and hematological health parameters. Thus, this result indicates that the plant proteins with the three limiting amino acids could replace FM up to 40% in diets for growing olive flounder.