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Comparison of immunogenecities of three beta-nodavirus proteins, capsid protein, non-structural protein B1 and B2 in olive flounder  

Cha, Seung-Ju (Pathology Division, National Fisheries Research & Development Institute)
Do, Jeong-Wan (Pathology Division, National Fisheries Research & Development Institute)
Ko, Myoung-Seok (Department of Biological Sciences, University of Ulsan)
Kim, Jin-Woo (Pathology Division, National Fisheries Research & Development Institute)
Park, Jeong-Woo (Department of Biological Sciences, University of Ulsan)
Publication Information
Journal of fish pathology / v.22, no.3, 2009 , pp. 219-228 More about this Journal
Abstract
The genomic and subgenomic RNAs of fish nodavirus encode the four proteins, protein A, capsid protein, non-structural protein B1 and B2. In this study, we describe the immune response of olive flounder Paralichthys olivaceus immunized with live fish nodavirus or recombinant capsid protein, non-structural protein B1 and B2 expressed in E. coli. Nodavirus-infected flounder produced antibodies to capsid protein, B1 and B2 and nodavirus-neutralizing activities were detected in the serum of the nodavirus-infected flounder. The flounder were immunized against the three recombinant proteins of fish nodavirus and the sera from these immunized fishes were assayed for nodavirus-specific antibody by ELISA and a neutralization test. In the immunized flounder, all three recombinant proteins induced the production of similar levels of antibody, but only the antibody to capsid protein significantly neutralized nodavirus. These results indicate that all three nodaviral proteins are immunogenic in flounder, but only the capsid protein can induce neutralizing antibody against nodavirus.
Keywords
Betanodavirus; Immunogenicity; Capsid protein; B1; B2; Flounder;
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1 Mady, B.J., Kurane, I., Erbe, D.V., Fanger, M.W. and Ennis, F.A.: Neuraminidase augments Fc$\gamma$ receptor II-mediated antibody-dependent enhancement of dengue virus infection. J. Gen. Virol., 74:839-844, 1993   DOI   ScienceOn
2 Schlesinger, J.J., Brandriss, M.W. and Walsh, E.E.: Protection of mice against dengue 2 virus encephalitis by immunization with dengue 2 virus non-structural glycoprotein NS1. J. Gen. Virol., 68:853-857, 1985   DOI   ScienceOn
3 Sommerset, I., Skern, R., Biering, E., Bleie, H., Fiksda, I.U., Grove, S. and Nerland, A.H.: Protection against Atlantic halibut nodavirus in turbot isinduced by recombinant capsid protein vaccination but not following DNA vaccination. Fish Shellfish Immunol., 18:13-29, 2005   DOI   ScienceOn
4 Tan, C., Huang, B., Chang, S.F., Ngoh, G.H., Munday, B., Chen, S.C. and Kwang, J.: Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain. J. Gen. Virol., 82:647-653, 2001   ScienceOn
5 Ball, L.A.: Requirements for the self-directed replication of flock house virus RNA 1. J. Virol., 69:720-727, 1995   ScienceOn
6 Cha, S.J., Do, J.W., Lee, N.S., An, E.J., Kim, Y.C., Kim, J.W. and Park, J.W.: Phylogenetic analysis of betanodaviruses isolated from cultured fish in Korea. Dis. Aquat. Organ., 77:181-189, 2007   DOI   ScienceOn
7 Chen, L.J., Su, Y.C. and Hong, J.R.: Betanodavirus non-structural protein B1: A novel antinecrotic death factor that modulates cell death in early replication cycle in fish cells. Virology, 385:444-454, 2009   DOI   ScienceOn
8 Krishna, N.K. and Schneemann, A.: Formation of an RNA heterodimer upon heating of nodavirus particles. J. Virol., 73:1699-1703. 1999   ScienceOn
9 Nagai, T. and Nishizawa, T.: Sequence of the nonstructural protein gene encoded by RNA1 of striped jack nervous necrosis virus. J. Gen. Virol., 80:3019-3022, 1999   ScienceOn
10 Bang, J.D., Kim, J.W., Lee, S.D., Park, S.I., Chun, S.G., Jeong, C.S. and Park, J.W.: Humoral immune response of flounder to Edwardsiella tarda: the presence of various sizes of immunoglobulins in flounder. Dis. Aquat. Organ., 26:197-203, 1996   DOI   ScienceOn
11 Su, Y.C., Wu, J.L. and Hong, J.R.: Betanodavirus non-structural protein B2: A novel necrotic death factor that induces mitochondriamediated cell death in fish cells. Virology 2008 Dec, 28, 2008
12 Fenner, B.J., Thiagarajan, R., Chua, H.K. and Kwang, J.: Betanodavirus B2 is an RNA interference antagonist that facilitates intracellular viral RNA accumulation. J. Virol., 80:85-94, 2006   DOI   ScienceOn
13 Li, H., Li, W.X. and Ding, S.W.: Induction and suppression of RNA silencing by an animal virus. Science, 296:1319-1321, 2002   DOI   ScienceOn
14 Halstead, S.B. and O'Rourke, E.J.: Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody. J. Exp. Med., 146:201-217, 1977   DOI   ScienceOn
15 Sommerset, I. and Nerland, A.H.: Complete sequence of RNA1 and subgenomic RNA3 of Atlantic halibut nodavirus (AHNV). Dis. Aquat. Organ., 58:117-125, 2004   DOI   ScienceOn
16 Friesen, P.D. and Rueckert. R.R.: Black beetle virus: messenger for protein B is a subgenomic viral RNA. J. Virol., 42:986-995, 1982   ScienceOn
17 Iwamoto, T., Mise, K., Takeda, A., Okinaka, Y., Mori, K., Arimoto, M., Okuno, T. and Nakai, T.: Characterization of Striped jack nervous necrosis virus subgenomic RNA3 and biological activities of its encoded protein B2. J. Gen. Virol., 86:2807-2816, 2005   DOI   ScienceOn
18 Johans, R., Ranheim, T., Hansen, M.K., Taksdal, T. and Totland, G.K.: Pathological changes in juvenile Atlantic halibut Hippoglossus hippoglossus persistently infected with nodavirus. Dis. Aquat. Organ., 50:161-169, 2002   DOI   ScienceOn
19 Li, W.X., Li, H., Lu, R., Li, F., Dus, M., Atkinson, P., Brydon, E.W., Johnson, K.L., García-Sastre, A., Ball, L.A., Palese, P. and Ding, S.W.: Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing. Proc. Natl. Acad. Sci., USA. 101:1350-1355, 2004   DOI   ScienceOn
20 Tanaka, S., Mori, K., Arimoto, M., Iwamoto, T. and Nakai, T.: Protective immunity of sevenband grouper, Epinephelus septemfasciatus (Thunberg), against experimental viral nervous necrosis. J. Fish Dis., 24:15-22, 2001   DOI
21 Russell, P.K., Nisalak, A., Sukhavachana, P. and Vivona, S.: A plaque reduction test for dengue virus neutralizing antibodies. J. Immunol., 99:285-290, 1967
22 Tirado, S.M. and Yoon, K.J.: Antibody-dependent enhancement of virus infection and disease. Viral Immunol., 16:69-86, 2003   DOI   ScienceOn
23 Zhang, Y.M., Hayes, E.P., McCarty, T.C., Dubois, D.R., Summers, P.L., Eckels, K.H., Chanock, R.M. and Lai, C.J.: Immunization of mice with dengue structural proteins and nonstructural protein NS1 expressed by baculovirus recombinant induces resistance to dengue virus encephalitis. J. Virol., 62:3027-3031, 1988   ScienceOn
24 Guarino, L.A., Ghosh, A., Dasmahapatra, B., Dasgupta, R. and Kaesberg, P.: Sequence of the black beetle virus subgenomic RNA and its location in the viral genome. Virology, 139:199-203, 1984   DOI   ScienceOn
25 Delsert, C., Morin, N. and Comps, M.: Fish nodavirus lytic cycle and semi-permissive expression in mammalian and fish cell cultures. J. Virol., 71:5673-5677, 1997   ScienceOn
26 Schneemann, A., Reddy, V. and Johnson, J.E.: The structure and function of nodavirus particles: a paradigm for understanding chemical biology. Adv. Virus Res., 50:381-446, 1998   DOI