Autotransgenic manipulation with a growth hormone (GH)-construct is a potential approach to improving the growth rates of farmed fish. Here, we present the generation of GH-autotransgenic common carp Cyprinus carpio carrying a transgene comprised of the carp homologous GH gene and a ${\beta}$-actin regulator. Autotransgenic carp showed similar viability to their non-transgenic siblings. Early growth characteristics of founder autotransgenic carp up to 50 days postfertilization were highly variable among individuals; i.e., some fish exhibited significant growth depression, while others showed dramatic acceleration of growth, achieving greater than sixfold increases in body weight relative to their non-transgenic counterparts. Stimulated growth performance became more notable with age and many transgenic individuals of the largest class reached 5 kg within 8 or 9 months, which is at least 10 times heavier than the average body weight of communally grown non-transgenics. Four of six founder transgenic males were successful in passing the transgene to their $F_1$ offspring with frequencies ranging from 19 to 36%. Growth stimulations were also persistent in all $F_1$ progeny groups examined.
The experiment was conducted t determine the effects of water temperature and daily feeding rate on Koran rockfish growth. The first experiment was carried out to know the growth of the fish in the different water temperature. The second experiment was carried out to know the effects of feeding rate of the fish. In the first experiment, feed efficiency was unaffected by the different water temprature 12, 15 and $18^{\circ}C$, but feed intake and growth rate were affected by the water temperature. Feed intake and growth rate of the fish reared in $15^{\circ}C$ were higher than those of the other groups. In the second experiment, feed efficiency was unaffected by feeding rate except the starve group. Daily growth rate of the fish fed 1.0% diet/BW or more. HSI, VSI, condition factor, GOT, GPT, TG, GL and Ht of fed groups were unaffected by the feeding rate in the experiment. Absoption ratios of protein and lipid were not different with the different feeding rates in fed fish groups. These results indicate that the optimum daily feeding rate of yearling Korean rockfish is about 70-80% of satiation rate.
Juhyoung Sung;Sungyoon Cho;Da-Eun Jung;Jongwon Kim;Jeonghwan Park;Kiwon Kwon;Young Myoung Ko
Journal of Internet Computing and Services
/
v.24
no.1
/
pp.61-69
/
2023
Recently, as the fishery resources are depleted, expectations for productivity improvement by 'rearing fishery' in land farms are greatly rising. In the case of land farms, unlike ocean environments, it is easy to control and manage environmental and breeding factors, and has the advantage of being able to adjust production according to the production plan. On the other hand, unlike in the natural environment, there is a disadvantage in that operation costs may significantly increase due to the artificial management for fish growth. Therefore, profit maximization can be pursued by efficiently operating the farm in accordance with the planned target shipment. In order to operate such an efficient farm and nurture fish, an accurate growth prediction model according to the target fish species is absolutely required. Most of the growth prediction models are mainly numerical results based on statistical analysis using farm data. In this paper, we present a growth prediction model from a stochastic point of view to overcome the difficulties in securing data and the difficulty in providing quantitative expected values for inaccuracies that existing growth prediction models from a statistical point of view may have. For a stochastic approach, modeling is performed by introducing a Gaussian process regression method based on water temperature, which is the most important factor in positive growth. From the corresponding results, it is expected that it will be able to provide reference values for more efficient farm operation by simultaneously providing the average value of the predicted growth value at a specific point in time and the confidence interval for that value.
Jeon, Joo Hyeon;Lee, Na Eun;Lee, Yoon Ho;Jang, Jea Moon;Joo, Moon Gab;Yoo, Byung Hwa;Yu, Jae Do
Journal of Information Processing Systems
/
v.17
no.6
/
pp.1179-1190
/
2021
A data acquisition and monitoring system was developed for an automated system of a smart fish farm. The fish farm is located in Jang Hang, South Korea, and was designed as circulation filtration system. Information of every aquaculture pool was automatically measured by pH sensors, dissolved oxygen sensors, and water temperature sensors and the data were stored in the database in a remoted server. Modbus protocol was used for gathering the data which were further used to optimize the pool water quality to predict the rate of growth and death of fish, and to deliver food automatically as planned by the fish farmer. By using JSON protocol, the collected data was delivered to the user's PC and mobile phone for analysis and easy monitoring. The developed monitoring system allowed the fish farmers to improve fish productivity and maximize profits.
Ra Jeong Chan;Kim Jeong Ho;Lee Jong Eun;Park Hyeong Geun;Kim Sung Hun;Kang Kyung Sun
Journal of Physiology & Pathology in Korean Medicine
/
v.17
no.3
/
pp.815-818
/
2003
Various kinds of chinese herbs have been known as to promote growth rate of animals. We investigated the growth promoting effect of the selected plants by using fish and rats as experimental animals. In a preliminary test by using fish, Eleutherococcus senticosus and Artemisia capillasis were found to be effective. Based on this result, we manufactured the mixture of plant extracts and applied this mixture to rice, named as Kiwoomi/sup TM/. When we administered Kiwoomi/sup TM/ to rats, it was found to be effective in growth promotion. Kiwoomi/sup TM/ - administered group showed 49.3% (0.75g/kg B.W.) and 52.3% (1.59/kg.B.W.) growth promotion, respectively. On the other hand, Control group showed 46.2% growth promotion. This growth promotion was supported by increase of GH and IGF-1 level in the Kiwoomi/sup TM/- administered group. From these results, we suggest that this functional rice could be helpful for growing children.
The pig slurry leachate was dark brown-colored solution that leaches out of woodchip trickling filter. The purpose of this research was to investigate the effect of pig slurry leachate and byproduct on growth characteristics of leaf lettuce in hydroponics culture. The effects of addition of fish meal, bone meal and crab shell for the growth of leaf lettuce were investigated. Leaf lettuce were grown in each of six combination treatment solutions; slurry leachate, slurry leachate + fish meal, slurry leachate+bone meal, slurry leachate + crab shell and chemical hydroponic solution for lettuce based on EC content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of lettuce. The all of nutrient solution was adjusted 1.5 mS/cm in EC in hydroponics culture. 1. The pH level of leachate of trickling filter was increased and EC decreased gradually during treatment. Pig slurry leachate was low in suspended solids (SS), phosphorus (P), but rich in potassium (K). 2. The plot of slurry leachate (SL) was lowest in the growth characteristics of lettuce. The leaf length and width of lettuce treated with mixture plot of slurry leachate and fish meal (SL + FM) was higher compared with plot in slurry leachate. The chlorophyll reading was reduced in plot treated with slurry leachate, but that in plot of SL+FM was similar compared with control plot. 3. The fresh weight of lettuce showed lowest in the plot treated with slurry leachate. The addition of fish meal increased the yield of comparing plot of slurry leachate, but plots of bone meal and crab shell addition were not significantly difference. The fresh weight of leaf lettuce in plot of SL+FM was 87% as 400.0g compared with control. In conclusion, the mixture solution of pig slurry leachate and fish meal could be used as a nutrition solution of organic lettuce hydroponics.
Azeezat Adenike Junaid;Mohd Salleh Kamarudin;Wahab Puteri Edaroyati;Quazim Olawale Junaid;Victor Tosin Okomoda;Mohammed Sani Isyaka;Yusuf Adewale Adejola;Danladi Mohammed Umar;Sarker Mohd Nurul Amin
Fisheries and Aquatic Sciences
/
v.26
no.8
/
pp.500-513
/
2023
The effects of prolonged photoperiod (additional night lighting) were investigated on the production of Ocimum basilicum (lemon basil) and fish/crayfish raised in a nutrient film technique aquaponic system. Hybrid lemon fin barb and red claw crayfish juveniles were co-cultured with O. basilicum and subjected to 12 h of ambient natural daylight and additional 0, 4, 8, and 12 h of night lighting for 14 weeks (two batches of 7 weeks each). The water quality parameters and the performance characteristics of the fish/crayfish/plant were evaluated, and the nutri- ent uptake (nitrogen, phosphorus, and potassium) in the system by the three organisms was also measured. The fish growth performance showed no significant differences (p > 0.05) in all the growth parameters measured across the treatments. The body proximate and nitrogen, phosphate, and potassium (NPK) composition of the fish and crayfish were not significantly (p > 0.05) different across the treatments. Similarly, there was no significant difference (p > 0.05) in the NPK uptake by the fish and crayfish across the treatments. The plant growth performance showed that there were no significant (p > 0.05) differences in the plant growth parameters measured, except for % plant height gained in the 12 h-light treatment (403.2 ± 26.0%) which was significantly (p < 0.05) higher than other treatments and cycles. Hence, this study demonstrat- ed that varying supplementary night lighting has no significant effect on the growth performance of O. basilicum, hybrid lemon fin barb, and red claw crayfish. This study, therefore, suggests that supplementary night lighting should not be considered for O. basilicum production as it does not significantly improve the performance characteristics of the plant nor the fish co-cultured with it in a polyculture aquaponic system.
Lee, Seung-Hyung;Yoo, Gwang-Yeol;Choi, Se-Min;Kim, Kang-Woong;Kang, Yong-Jin;Bai, Sung-Chul C.
Journal of Aquaculture
/
v.21
no.2
/
pp.82-88
/
2008
A 12-week feeding trial was conducted to investigate the effects of dietary supplementation of probiotics as a feed additive for juvenile Korean rockfish Sebastes schlegeli. Four experimental diets supplemented with no probiotic(Control), Bacillus polyfermenticus(BP), Bacillus licheniformis(BL) or Bacillus polyfermenticus plus Saccharomyces cerevisiae(BP+SC) at $1.0{\times}10^7$ CFU/kg diet as a dry-mater(DM) basis were prepared by mixing with a basal diet. After 12 weeks of the feeding trial, fish fed BP+SC diet showed significantly higher weight gain(WG), feed efficiency(FE), specific growth rate(SGR) and protein efficiency ratio(PER) than those of fish fed control diet(P<0.05), however there were no significant differences in WG, FE, SGR and PER among fish fed the BP, BL and BP+SC diets. Fish fed BP and BP+SC diets showed significantly higher condition factor(CF) than that of fish fed control and BL diets. Fish fed BP, BL, BP+SC diets showed significantly higher hepatosomatic index(HSI) than that of fish fed control diet, however there was no significant difference in HSI among fish fed BP, BL and BP+SC diets. Fish fed BP+SC diet showed significantly lower serum glucose than that of fish fed control diet, however there was no significant difference in serum glucose among fish fed BP, BL and BP+SC diets. Fish fed BP+SC diet showed significantly higher respiratory burst activity(NBT assay) than that of the fish fed control and BL diets, however there was no significant difference in NBT assay between fish fed BP and BP+SC diets. Fish fed BP and BL diets showed significantly higher lysozyme activity than that of the fish fed control diet, however there was no significant difference in lysozyme activity among fish fed BP, BL and BP+SC diets. Fish fed BP and BP+SC diets showed significantly lower cumulative mortality than that of the fish fed control diet, however there was no significant difference in cumulative mortality among fish fed BP, BL and BP+SC diets after the challenge test. From these results, dietary B. polyfermenticus, B. licheniformis and B. polyfermenticus plus S. cerevisiae supplementation in juvenile Korean rockfish diet could enhance growth performances, non-speicific immunities and a higher resistance against the specific pathogen.
Proceedings of the Korean Aquaculture Society Conference
/
2003.10a
/
pp.78-79
/
2003
Effects of dietary lipid level and source (squid liver oil being rich in n-3 HUFA, soybean oil being rich in 18:2n-6, and linseed oil being rich in 18:3n-3) in fishmeal-based diet on growth and body composition of grower sunshine bass raised in seawater were investigated. Fifteen grower (an initial weight of 146.8$\pm$0.23 g) sunshine bass were randomly distributed into 27 of 250 L fiber reinforced plastic flow-through tanks. Fish were hand-fed to satiety twice daily for 6 days a week throughout the feeding trial. Survival was over 97% and not significantly affected by either dietary lipid level or lipid source (n-3 highly unusaturated fatty acid, HUFA). Weight gain of fish tended to improve with dietary n-3 HUFA level up to 2.9%, but sharply decreased at 3.5%. The best weight gain was obtained in fish fed the diet supplemented with 6% squid liver oil and 3% soybean oil. FER and PER were not significantly affected by either dietary lipid level or dietary lipid source. The lowest moisture content of the whole body was observed in fish fed the diet supplemented with 12% squid liver oil and highest for the diet supplemented with 9% linseed oil, respectively. Protein content of fish was not significantly affected by either dietary lipid level or dietary lipid source. However, lipid content of the whole fish tended to increase with an increase of either dietary lipid level or dietary n-3 HUFA level, except for fish fed the diet supplemented with 9% linseed oil. Ash content of fish fed the diet with no supplementation of oil was highest and lowest for the diet supplemented with 9% soybean oil, respectively. Significant differences in saturated fatty acids (16:0, 18:0 and 24:0), monoene (18:1n-9), 18:2n-6, 20:5n-3 and sum of n-3 HFUA of fish were observed. In considering these results, it could be concluded that supplementation of 9% oil combined with 6% squid liver oil and 3% soybean oil into fishmeal-based diet was the most recommendable for growth of grower sunshine bass raised in seawater.
Kim Pyong-Kih;Myoung Jung-Goo;Kim Jong-Man;Huh Hyung Tak;Kim Hyung Bae
Journal of Aquaculture
/
v.3
no.2
/
pp.127-133
/
1990
Growth Comparisons of two age groups of coho salmon, Oncorhynchus kisutch, underyearling and age one, were made at Chungmu Experimental Fish Culture Station from December 17, 1988 to April 26, 1989. During the first 66 days of experimental period, the fish of underyearling group grew from 167.0 g to 633.1 g in average weight and the feed coefficient (FC) and daily growth rate (DGR) were 1.1 and $2.0\%$, respectively. In the same period, the fish of age one group grew from 396.0 g to 854.6 g in average weight and the FC and DGR were 1.1 and $2.0\%$, respectively. During the second 63 days, the fish of underyearling and age one group grew up to 1171.9 g and 1239.7 g respectively. FCs of these two groups were 1.5 and 2.6, respectively and DGRs were $1.0\%$ and $0.7\%$, respectively. Underyearling fish consumed more feed and performed better FC and DGR than age one fish. FC and DGR sharply decreased as the fish weight increased. Therefore under-yearling fish of this species seems better as seeds for seawater cage culture. For this purpose it is necessary to increase smolting rate in the underyearling group fish within a single season.
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