Browse > Article

Effects of Dietary Probiotics Supplementation on Juvenile Korean Rockfish Sebastes schlegeli  

Lee, Seung-Hyung (Department of Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University)
Yoo, Gwang-Yeol (Fishery Research Center)
Choi, Se-Min (Aquafeed Research Center, East Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Kim, Kang-Woong (Aquafeed Research Center, East Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Kang, Yong-Jin (Aquafeed Research Center, East Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Bai, Sung-Chul C. (Department of Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University)
Publication Information
Journal of Aquaculture / v.21, no.2, 2008 , pp. 82-88 More about this Journal
Abstract
A 12-week feeding trial was conducted to investigate the effects of dietary supplementation of probiotics as a feed additive for juvenile Korean rockfish Sebastes schlegeli. Four experimental diets supplemented with no probiotic(Control), Bacillus polyfermenticus(BP), Bacillus licheniformis(BL) or Bacillus polyfermenticus plus Saccharomyces cerevisiae(BP+SC) at $1.0{\times}10^7$ CFU/kg diet as a dry-mater(DM) basis were prepared by mixing with a basal diet. After 12 weeks of the feeding trial, fish fed BP+SC diet showed significantly higher weight gain(WG), feed efficiency(FE), specific growth rate(SGR) and protein efficiency ratio(PER) than those of fish fed control diet(P<0.05), however there were no significant differences in WG, FE, SGR and PER among fish fed the BP, BL and BP+SC diets. Fish fed BP and BP+SC diets showed significantly higher condition factor(CF) than that of fish fed control and BL diets. Fish fed BP, BL, BP+SC diets showed significantly higher hepatosomatic index(HSI) than that of fish fed control diet, however there was no significant difference in HSI among fish fed BP, BL and BP+SC diets. Fish fed BP+SC diet showed significantly lower serum glucose than that of fish fed control diet, however there was no significant difference in serum glucose among fish fed BP, BL and BP+SC diets. Fish fed BP+SC diet showed significantly higher respiratory burst activity(NBT assay) than that of the fish fed control and BL diets, however there was no significant difference in NBT assay between fish fed BP and BP+SC diets. Fish fed BP and BL diets showed significantly higher lysozyme activity than that of the fish fed control diet, however there was no significant difference in lysozyme activity among fish fed BP, BL and BP+SC diets. Fish fed BP and BP+SC diets showed significantly lower cumulative mortality than that of the fish fed control diet, however there was no significant difference in cumulative mortality among fish fed BP, BL and BP+SC diets after the challenge test. From these results, dietary B. polyfermenticus, B. licheniformis and B. polyfermenticus plus S. cerevisiae supplementation in juvenile Korean rockfish diet could enhance growth performances, non-speicific immunities and a higher resistance against the specific pathogen.
Keywords
Paralichthys olivaceus; probiotics; growth performances; non-specific immune responses; challenge test; Korean rockfish;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bougon, M., M. Launay and M. Le Menec, 1988. Influence d'un probiotique, l'Biocroissance, sur les performances des pondeuses. Bull. Inf. Stn. Exp. Avicult. Ploufragan 28, 110-115
2 Chen, D. and A. J. Ainsworth, 1992. Glucan administration potentiates immune defense mechanisms of channel catfish, Ictalurus punctatus Rafinequ. J. Fish Dis., 15, 295-304   DOI
3 Gatesoupe, F. J., 1999. The use of probiotics in aquaculture. Aquaculture, 180, 147-165   DOI   ScienceOn
4 Ingram, G. A., 1980. Substances involved in the natural resistance of fish to infection - a review. J. Fish Biol., 16(1), 23-60   DOI
5 Lee, S. M. and J. Y. Lee, 1994. Effect of dietary $\alpha$-cellulose levels on the growth, feed efficiency and body composition in Korean rockfish, Seabastes schlegeli. J. Aquacult., 7(2), 97-107
6 Maurilio, Lara-Flores, M. A. Olvera-Novoa, B. E. Guzman-Mendez and W. Lopez-Madrid. 2003. Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture, 216, 193-201   DOI   ScienceOn
7 Naik, A. T. R., H. S. Murthy and T. J. Ramesha, 1999. Effect of graded levels of G-probiotic on growth, survival and feed conversion of tilapia, Oreochromis mossambicus. Fish. Technol., 36, 63-66
8 O'Sullivan, D. J., 1999. Methods of analysis of the intestinal microflora. (in) Tannock, G.W. (ed.), Probiotics: A critical review. Horizon Scientific Press, Wymondham, England, pp. 23-44
9 Park, G. J., 2004. Development of the growth and immune stimulants in olive flounder, Paralichthys olivaceus. Ph.D. thesis Pukyong National University, Busan, Korea
10 Ringo, E., T. H. Birkbeck, P. D. Munro, O. Vadstein, K. Hjelmeland, 1996. The effect of early exposure to Vibrio pelagius on the aerobic bacterial flora of turbot, Scophthalmus maximus L. larvae. J. Appl. Bacteriol., 81, 207-211   DOI
11 Sakai, M., M. Kobayashi and H. Kawauchi, 1996. Mitogenic effects of growth hormone and prolactin on chum salmon, Onchorhynchus keta leucocytes in vitro. Vet. Immunol. Immunopathol., 53, 185-190   DOI   ScienceOn
12 Taoka, Y., H. Maeda, J. Y. Jo, M. J. Jeon, S. C. Bai, W. J. Lee, K. Yuge and S. Koshio, 2006. Growth, stress tolerance and nonspecific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system. Fish. Sci., 72, 310-321   DOI   ScienceOn
13 Vaquez-Juarez, R., F. Ascensio, T. Andlid, L. Gustafsson and T. Wadstrom, 1993. The expression of potential colonisation factors of yeasts isolated from fish during different growth conditions. Can. J. Microbiol., 39, 1135-1141   DOI   ScienceOn
14 Yoo, G., S. Lee, G. J. Park, S. M. Choi, O. E. Okorie, Y. C. Kim, K. W. Kim, Y. J. Kang and S. C. Bai, 2007b. Effects of dietary recombinant bovine somatotropin levels on growth, plasma recombinant bovine somatotropin concentrations, and body composition of juvenile Korean rockfish, Sebastes schlegeli. J. World Aquacult. Soc., 38(2), 200-207   DOI   ScienceOn
15 Shiri Harzevili, A. R., H. Van Duffel, P. Dhert, J. Swings, and P. Sorgeloos, 1998. Use of a potential probiotic Lactococcus lactis AR21 strain for the enhancement of growth in the rotifer Brachionus plicatilis Muller. Aquacult. Res., 29, 411-417
16 Yoo, G., S. Lee, Y. C. Kim, Y. C. Kim, O. E. Okorie, G. J. Park, Y. O. Han, S. M. Choi, J. C. Kang, M. Sun and S. C. Bai, 2007a. Effects of dietary $\beta$,-1,3 glucan and feed stimulants in juvenile olive flounder, Paralichthys olivaceus. J. World Aquacult. Soc., 38(1), 138-145   DOI   ScienceOn
17 Hansen, N. E., 1974. Plasma lysozyme a measure of neutrophil turnover. An analytical review. Acta Haematol., 7, 7-87
18 NRC (National Research Council), 1993. Nutrient Requirements of Warmwater Fishes and Shellfishes. National Acad. Press, Washington, D.C., 102 pp
19 Jeong, C. W., H. J. Choi, G. Yoo, S. Lee, Y. C. Kim, O. E. Okorie, J. H. Lee, K. D. Jun, S. M. Choi, K. W. Kim, Y. J. Kang, J. C. Kang, I. S. Kong and S. C. Bai, 2006. Effects of dietary probiotics supplementation on juvenile olive flounder, Paralichthys olivaceus. J. Kor. Fish. Soc., 39(6), 460-465   과학기술학회마을   DOI
20 Bogut, I., Z. Milakovic, Z. Bukvic, S. Brkic and R. Zimmer, 1998. Influence of probiotic (Streptococcus faecium M74) on growth and content of intestinal microflora in carp (Cyprinus carpio). Czech. J. Anim. Sci., 43, 231-235
21 Kennedy, S. B., J. W. Tucker, C. L. Neidic, G. K. Vermeer, V. R. Cooper, J. L. Jarrell and D. G. Sennett, 1998. Bacterial management strategies for stock enhancement of warm water marine fish: a case study with common snook (Centropomus undecimalis). Bull. Mar. Sci., 62, 573-588
22 De Schrijver, R. and F. Ollevier, 2000. Protein digestion in juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus. Aquaculture, 186, 107-116   DOI   ScienceOn
23 Anderson, D. P., B. S. Robertson and O. W. Dickson, 1979. Cellular immune response in rainbow trout, Salmon gairdneri Richardson to Yersinia ruckeri O-antigen monitored by the passive haemolytic plaque assay test. J. Fish Dis., 2(3), 169-178   DOI
24 AOAC (Association of Official Analytical Chemists). 2000. Cunniff, P.(ed.), Official Methods of Analysis of the Association of Official Analytical Chemists, 16th edition. Association of Official Analytical Chemists, Inc., Arlington, VA
25 Brown, B. A., 1980. Routine hematology procedures. (in) Hematology: Principles and Procedures. pp. 71-112. Lea and Febiger, Philadelphia. Bull. Freshwater Fish. Res. Lab., 12, 1-4
26 Mohanty, S. N., S. K. Swain and S. D. Tripathi, 1996. Rearing of catla (Catla catla Ham.) spawn on formulated diets. J. Aquac ult. Tropics, 11, 253-258
27 Rychen, G. and S. Nunes, 1994. Effects of three microbial probiotics on postprandial concentration differences of glucose, galactose and amino-nitrogen in the young pig. Rep. Cent. Recher. Nut. Anim. Soc. Chimi Roche, pp. 107-111
28 Griffin, B. R., 1983. Opsonic effect of rainbow trout (Salmo gairdneri) antibody on phagocytosis of Yersinia ruckeri by trout leukocytes. Develop. and Comp. Immunol., 7, 253-259   DOI   ScienceOn
29 Statistical Year Book of Maritime Affairs and Fisheries, 2005. Statistical year book maritime affairs and fisheries. Ministry of Maritime Affairs and Fisheries, Korea
30 Austin, B., 1998. Biotechnology and diagnosis and control of fish pathogens. J. Mar. Biotechnol. 6, 1-2
31 Chang, C. I. and W. Y. Liu, 2002. An evaluation of two probiotic bacterial strains, Enterococcus faecium SF68 and Bacillus toyoi, for reducing Edwardsiellosis in cultured European eel, Anguilla anguilla L. J. Fish Dis., 25, 311-315   DOI   ScienceOn
32 Olesen, N. J., 1991. Detection of the antibody response in rainbow trout following immersion vaccination with Yersinia ruckeri bacterin by ELISA. J. Appl. Ichthyol., 7, 36-43   DOI
33 Jun, K. D., H. J. Kim, K. H. Lee, H. D. Paik and J. S. Kang, 2002. Characterization of Bacillus polyfermenticus SCD as a probiotic. Kor. J. Microbiol. Biotechnol., 30(4), 359-366
34 Secombes C. J., 1990. Isolation of salmonid macrophages and analysis of their Killing Activity. (in) J. S. Stolen, T. C. Fletcher, D. P. Anderson, B. S. Roberson and W. B. van Muiswinkel (eds.), Techniques in Fish Immunology, No. 1, SOS Publications, pp. 137-154