• 환경생물
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• 제36권3호
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• pp.352-358
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• 2018

본 연구는 PCR 기반 RFLP (Restriction Fragment Length Polymorphism; 제한절편 길이 다형성) 분자기법을 활용하여 난 및 치어 대상 납자루아과 어류 3종의 동정을 좀 더 빠르고 정확하게 파악하고 납자루아과 어류의 종별 산란양상 및 번식생태 이해에 대한 기여가 목적이다. 본 연구를 위해 기존 선행된 문헌자료를 확인하고 납자루아과 어류가 2종 이상 동서하고 있는 지역을 확인하여 현지조사를 수행하였다. 현지조사 결과 확인된 납자루아과 어류는 묵납자루(Acheilognathus signifer), 줄납자루(A. yamatsutae) 및 각시붕어(Rhodeus uyekii)로 총 3종이 확인되었으며, 확인된 납자루아과 어류와 동서하고 있는 숙주조개(작은말조개; Unio douglasiae sinuolatus)를 채집하여 숙주조개 속 납자루아과 어류의 난 및 치어를 확보하였다. 현지조사 결과 확인된 납자루아과 어류 3종을 대상으로 미토콘드리아 DNA COI과 cyt b 유전자 염기서열을 비교하여 각각 종별로 특이성을 지닌 부위(단일염기변이; Single Nucleotide Variation: SNV)에 맞는 제한효소를 선정하였고, 숙주조개 속 난 및 치어를 대상으로 genomic DNA를 추출하여 PCR-RFLP 실험을 수행한 결과 현지조사 시 확인된 납자루아과 어류 3종의 독특한 제한절편 길이 양상을 전기영동을 통하여 확인하였다. 본 연구를 통해 묵납자루, 줄납자루 및 각시붕어의 종을 판별할 수 있는 RFLP 마커를 개발하였으며, 숙주조개 난 및 치어를 대상으로 정확한 종의 동정을 보다 빠르고 효과적으로 수행하여 각각 납자루아과 종별 산란양상을 보다 정확히 규명하고 향후 이들 자연개체군의 효과적인 유지, 관리 및 보전 방법 개발에 유용하게 활용될 수 있을 것으로 판단된다.

• 한국어병학회지
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• 제36권2호
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• pp.403-408
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• 2023

본 연구를 통해 한국의 양식 가숭어에서 최초로 분리된 등각류는 Nerocila japonica와 가장 근연한 종으로 확인되었으나, 향후 형태학적 분류 등 명확한 종 동정이 추가로 필요할 것으로 판단되며, 약물의 직접 노출에 의한 구충 효과를 확인한 결과 trichlorfon이 가장 효과가 있는 것으로 확인되었다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.144-145
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• 2001

Marine microalgae are used extensively in mariculture as food for marine animals, in particular larval and juvenile molluscs, crustaceans and fish. A wide range of microalgae has been tested, because not all species are equally successful in supporting growth of a particular animal. In addition, bioactivities of marine microalgae are recently investigated for the effective exploitation of unutilized marine resources. (omitted)

• Fisheries and Aquatic Sciences
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• 제3권3_4호
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• pp.228-229
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• 2000

Trypanosoma sp. (Sarcomastigophora: Kinetoplastida) was found in the blood of the Korean catfish, Silurus asotus, for the first time in Korea. The morphological characteristics of Trypanosoma sp. in the present study were similar with those of T. carassii, T. tincae and T. danilewskyi. However, the free flagellum length of Trypanosoma sp. was obviously shorter than that of those species. The species identification was reserved until elucidating the pleomor-phism according to the phase of infection and the cross infectivity among fish species.

• 한국식품위생안전성학회지
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• 제35권6호
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• pp.574-580
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• 2020

본 연구에서는 민어과 수산물의 표준시료 DNA 염기서열을 분석한 후 DNA barcode 염기서열을 확정하고 검증한 후 시중 유통 중인 민어과 수산가공식품의 위변조 현황을 조사하였다. 표준시료의 미토콘드리아 cytochrome c oxidase subunit I유전자를 증폭한 후 증폭된 PCR 산물의 염기서열을 분석하였다. 분석결과 종 판별에 특이적인 655 bp를 선정하여 DNA barcode 염기서열로 하였다. DNA barcode information과 primer set을 이용한 진위판별 정확성을 확인한 결과 PCR 증폭은 모두 확인되었다. NCBI에 등록된 각각 어류의 유전자 염기서열과 비교하였을 때 참조기 100%, 부세 100%, 보구치 100%, 민어 100%의 상동성을 나타내어 실험에 사용된 DNA barcode information과 primer set의 정확성을 확인하였다. DNA barcode 시험법을 이용해 시중 유통되는 민어과 수산가공품 32건에 대해 조사한 결과 위변조 사례는 나타나지 않았다. 그러나 식품공전에 등재된 보구치 대신 백조기라는 일반명이 사용되고 있어 소비자에게 혼란을 야기하고 있었다. 따라서 수산가공품 원재료 표시에 일반명과 더불어 표준명 또는 학명을 표시하여야 할 것으로 판단되었다.

• Animal Systematics, Evolution and Diversity
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• 제2권1호
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• pp.63-78
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• 1986

Two species of Moroco oxycephalus and M. lagowskii were studied to examine patterns of genic variation and morphological difference within and between populations of two species, and to clarify their taxonomic status and geographic distribution. The number of scales above lateral line(SAL) was the key character to classify these species. On the average, M.lagowskii had 22 or more SAL whereas M.oxycephalus had 20 or less. Previously known character of the position of dorsal fin was found to be not appropriate to distinguish them. Five loci, Gp, st-1, Est-2, Est-3 and Got-1 , showed fixed difference electrophoretically between two species and these could be used as genetic markers to identify them. The degree of genic variation of M. oxycephalus was four fold higher(H=0.032) than that of M. lagowskii(H=0.008) but both species were far less than the average genic variation of freshwater fish in general. Rogers' genetic similarity coefficients between two species were S=0.692 and their presumed divergent time was estimated to be sbout 1.8million years ageo. Detailed survey of the geographic distribution of thses revealed that M.lagowskii was distributed in northeastern part of South Korea(Ganseong , Gangreung, Wangsan, and Oggye) and M. oxycephalus was occupied rest of the peninsula. The distrance between Oggye, the southern limit of M. lagowskii distribution , and Samwha (near Samcheog), the northern limit of M.oxycephalus, was aobut 15 Km apart and no symparty was found in between.

• 한국어병학회지
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• 제34권1호
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• pp.105-110
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• 2021

We experienced mortality of dark-banded rockfish (Sebastes inermis) maintained in the rearing facility located in Gangneung, after transportation. Moribund fish showed various symptoms such as exophthalmia, skin ulcers, tail rots, gill rots, discoloration of liver with petechiae, yellowish fluid in intestines and ascites. Two different colonies were dominantly appeared after spreading the lesions on the agar plates and incubation. One isolate (SI_1) showed swarming movement on TSA, and formed yellow colonies on TCBS agar. The other (SI_2) showed no swarming motility and green colonies on TCBS agar. Both of them were Gram-negative. All of these results are similar with those of Genus Vibrio. They were identified as V. harveyi and V. gigantis by PCR with subsequent sequencing of 3 different genes (16 rDNA, recA, rpoA). V. harveyi is well-known as a serious pathogen of marine fish and invertebrates, while V. gigantis is known to be often isolated from marine invertebrates, but the pathogenicity is still unknown. We suspect V. harveyi as the cause of the mortality of dark-banded rockfish, but challenge experiments with these 2 Vibrio species are thought to be necessary to make a clear conclusion.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.569-581
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• 2023

The morphology and genetic identification of Rasbora lateristriata and Rasbora argyrotaenia between cultivated and wild populations has never been reported. This study compares morphology and cytochrome c oxidase (COI) genes between farmed and wild stock Rasbora spp. in Java and Sumatra island, Indonesia. We analyzed the truss network measurement (TNM) characters of 80 fish using discriminant function analysis statistical tests. DNA was extracted from muscle tissue of 24 fish specimens, which was then followed by polymerase chain reaction, sequencing, phylogenetic analysis, fixation index analysis, and statistical analysis of haplotype networks. Basic Local Alignment Search Tool analysis validated the following species: R. lateristriata and R. argyrotaenia from farming (Jogjakarta); Rasbora agryotaenia (Purworejo), R. lateristriata (Purworejo and Malang), Rasbora dusonensis (Palembang), and Rasbora einthovenii (Riau) from natural resources. Based on TNM characters, Rasbora spp. were divided into four groups, referring to four distinct characters in the middle of the body. The phylogenetic tree is divided into five clades. The genetic distance between R. argyrotaenia (Jogjakarta) and R. lateristriata (Malang) populations (0.66) was significantly different (p < 0.05). R. lateristriata (Purworejo) has the highest nucleotide diversity (0.43). R. argyrotaenia from Jogjakarta and Purworejo shared the same haplotype. The pattern of gene flow among them results from the two populations' close geographic proximity and environmental effects. R. argyrotaenia had low genetic diversity, therefore, increasing heterozygosity in cultivated populations is necessary to avoid inbreeding. Otherwise, R. lateristriata (Purworejo) had a greater gene variety that could be used to develop breeding. In conclusion, the middle body parts are a distinguishing morphometric character of Rasbora spp., and the COI gene is more heterozygous in the wild population than in farmed fish, therefore, enrichment of genetic variation is required for sustainable Rasbora fish farming.

• Molecules and Cells
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• 제23권3호
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• pp.379-390
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• 2007

We sequenced and characterized the complete mitochondrial genome of the Japanese fish tapeworm D. nihonkaiense. The genome is a circular-DNA molecule of 13607 bp (one nucleotide shorter than that of D. latum mtDNA) containing 12 protein-coding genes (lacking atp8), 22 tRNA genes and two rRNA genes. Gene order and genome content are identical to those of the other cestodes reported thus far, including its congener D. latum. The only exception is Hymenolepis diminuta in which the positions of trnS2 and trnL1 are switched. We tested a PCR-based molecular assay designed to rapidly and accurately differentiate between D. nihonkaiense and D. latum using species-specific primers based on a comparison of their mtDNA sequences. We found the PCR-based system to be very reliable and specific, and suggest that PCR-based identification methods using mtDNA sequences could contribute to the study of the epidemiology and larval ecology of Diphyllobothrium species.

• 한국어류학회지
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• 제34권1호
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• pp.1-7
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• 2022

북서태평양 표층에서 채집한 후기 자어 (2.2 mm BL)의 미토콘드리아 전장 유전체를 근거로 Himantolophus groenlandicus로 동정하였다. 후기 자어는 둥근 몸통, 작은 이빨, 발달하기 시작한 등 지느러미, 아래 부분이 들어간 눈, 그리고 지느러미, 정수리, 등 부분에 분포하는 흑색소포를 가졌다. 이러한 특징은 크기가 유사한 동종으로 보고된 자어(2.1 mm BL)와 차이가 컸다. 본 연구 자어의 유전적, 형태적 특징은 유사종의 동정에 유용할 것이다.