• 제목/요약/키워드: Fine Mapping

검색결과 128건 처리시간 0.036초

SSR Marker Linked to f Locus in Soybean

  • Nam, Ki-Chul;Kim, Myung-Sik;Jeong, Woo-Hyeun;Kim, Seok-Hyeon;Chung, Jong-Il
    • 한국작물학회지
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    • 제52권1호
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    • pp.51-54
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    • 2007
  • Soybean has a morphological type with a broadened and flattened stem. Fasciation has been suggested as a new gene for soybean research. SSR marker linked to the $\Large f$ locus that controls fasciation phenotype has not identified within 10 cM. A mapping population consisting of 94 $F_2$ progenies was derived from a cross between wild type Clark (FF) and fasciation mutant C32 (${\Large f}{\Large f}$). The phenotype of $F_2$ individual plants was recorded at R2 and R3 growth stage from field. One-thousand 10-mer oligonucleotide RAPD primers and 29 SSR primers selected from the D1b+W of the soybean molecular linkage map were used. A genetic map was constructed from the segregating 35 RAPD, four SSR markers and one phenotypic(wild type/fasciation) marker. The segregation ratios of 3 : 1 observed in the $F_2$ population and the Chi-square values strongly suggest that the fasciation trait is controlled by a single recessive gene. Satt537 marker was linked to $\Large f$ locus at a distance of 9.6 cM. Assignment of the $\Large f$ locus to linkage group D1b+W and identification of markers can be used as an initial step for fine mapping of the $\Large f$ gene.

펨토초 레이저의 이송속도에 따른 Invar 합금의 어블레이션 특성 (Characteristics of the Femto-second Pulsed Laser Ablation according to Feed Velocity on the Invar Alloy)

  • 정일영;강경호;김재도;손익부;노영철;이종민
    • 한국정밀공학회지
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    • 제26권3호
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    • pp.25-31
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    • 2009
  • Femto-second laser ablation with the various feed velocities of the Invar alloy and the micro surface milling for the processing condition were studied. We used a regenerative amplified Ti:sapphire laser with a 1kHz repetition rate, 184fs pulse duration time and 785nm wavelength. Femto-second laser pulse was irradiated on the Invar alloy with the air blowing at the condition of various laser peak powers and feed velocities. An ablation characteristic according to feed velocity of the Invar alloy was appeared as the non-linear type at different zone of energy fluence. The micro surface milling of the Invar alloy using a mapping method was investigated. The optimal condition of micro surface milling was laser peak power of 22.8mW, feed velocity of 1 mm/s, beam gap of $1{\mu}m$. With the optimal processing condition, the fine rectangular shape without burr and thermal damage was achieved. Using the femto-second laser system, it demonstrates excellent tool for micro surface milling of the Invar alloy without heat effects and poor edge.

웹 서비스 기반 GIS 연동 폭풍.해일 시각화 시스템 개발 (Development of a Web Service based GIS-Enabled Storm-surge Visualization System)

  • 김진아;박진아;박광순;권재일
    • 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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    • 제14권9호
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    • pp.841-849
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    • 2008
  • 최근 지구온난화 및 해수면 상승으로 인한 태풍의 내습빈도와 강도의 증가로 태풍 내습시 연안지역의 침수 범람과 같은 자연재해로 인한 국민의 재산과 인명 피해가 급증하고 있다. 이에 폭풍 해일 예측을 위한 수치모델의 수립과 개선을 통하여 해일의 높이 및 발생시간, 해일로 인한 침수.범람 지역을 보다 과학적으로 정확하게 예측하려는 연구가 활발히 진행되고 있다. 따라서 본 논문에서는 이러한 예측결과를 일반 국민들에게 보다 효과적으로 전달하여, 연안재해로 인한 피해를 예방하기 위하여 웹 서비스 기반 GIS 연동 폭풍 해일 시각화 시스템을 개발하였다. 또한 수치모델의 정확한 예측결과 및 연안지역의 정밀 지리정보 구축을 위하여 LiDAR 자료를 이용한 GIS 기반 육도-해도 접합을 통한 연안지역 정밀해상도의 DEM을 생성하였다.

Quantitative Trait Locus Mapping and Candidate Gene Analysis for Plant Architecture Traits Using Whole Genome Re-Sequencing in Rice

  • Lim, Jung-Hyun;Yang, Hyun-Jung;Jung, Ki-Hong;Yoo, Soo-Cheul;Paek, Nam-Chon
    • Molecules and Cells
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    • 제37권2호
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    • pp.149-160
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    • 2014
  • Plant breeders have focused on improving plant architecture as an effective means to increase crop yield. Here, we identify the main-effect quantitative trait loci (QTLs) for plant shape-related traits in rice (Oryza sativa) and find candidate genes by applying whole genome re-sequencing of two parental cultivars using next-generation sequencing. To identify QTLs influencing plant shape, we analyzed six traits: plant height, tiller number, panicle diameter, panicle length, flag leaf length, and flag leaf width. We performed QTL analysis with 178 $F_7$ recombinant inbred lines (RILs) from a cross of japonica rice line 'SNU-SG1' and indica rice line 'Milyang23'. Using 131 molecular markers, including 28 insertion/deletion markers, we identified 11 main- and 16 minor-effect QTLs for the six traits with a threshold LOD value > 2.8. Our sequence analysis identified fifty-four candidate genes for the main-effect QTLs. By further comparison of coding sequences and meta-expression profiles between japonica and indica rice varieties, we finally chose 15 strong candidate genes for the 11 main-effect QTLs. Our study shows that the whole-genome sequence data substantially enhanced the efficiency of polymorphic marker development for QTL fine-mapping and the identification of possible candidate genes. This yields useful genetic resources for breeding high-yielding rice cultivars with improved plant architecture.

Quantitative Trait Loci Mapping for Porcine Backfat Thickness

  • Wu, X.L.;Lee, C.;Jiang, J.;Peng, Y.L.;Yan, H.F.;Yang, S.L.;Xiao, B.N.;Liu, X.C.;Shi, Q.S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권7호
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    • pp.932-937
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    • 2002
  • A partial genome scan using porcine microsatellites was carried out to detect quantitative trait loci (QTL) for backfat thickness (BFT) in a pig reference population. This population carried QTL on chromosomes 1, 13 and 18. The QTL on chromosome 1 was located between marker loci S0113 and SW1301. The QTL corresponded to very low density lipoprotein receptor gene (VLDLR) in location and in biological effects, suggesting that VLDLR might be a candidate gene. The QTL found on chromosome 13 was found between marker loci SWR1941 and SW864, but significance for the marker-trait association was inconsistent by using data with different generations. The QTL on chromosome 18 was discovered between markers S0062 and S0117, and it was in proximity of the regions where IGFBP3 and GHRHR were located. The porcine obese gene might be also a candidate gene for the QTL on chromosome 18. In order to understand genetic architecture of BFT better, fine mapping and positional comparative candidate gene analyses are necessary.

QTL analysis of for micronutrient content in rice grain

  • Lee, Hyun-Sook;Shim, Kyu-Chan;Jeon, Yun-A;Ahn, Sang-Nag
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.144-144
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    • 2017
  • Micronutrients such as zinc (Zn), iron (Fe), manganese (Mn) have important roles for development and growth in plants but it also have roles in animals and humans. In previous studies, a Korean weedy rice, KH2J was selected to have tolerance to heavy metal, lead (Pb) compared with a cultivar, Milyang23. To identify QTLs for micronutrients concentration in grain, an F2 population (120 plants) were developed from a cross between KH2J and an indica rice cultivar, Milyang23. To measure the concentration of eight ions, Zn, Fe, Mn, Pb, calcium (Ca), copper (Cu), cadmium (Cd) and arsenic (As), grains were collected and digested with 65% nitric acid, and the ion contents were measured using inductively coupled plasma mass spectrometry. A total 27 putative quantitative trait loci (QTLs) were detected on 12 chromosomes by single point analysis and 22 putative QTLs were detected by composite interval mapping. The co-locations of QTL for Zn, Fe and Mn were observed on chromosome 5. The QTLs for Cd, Cu and Zn were co-localized on chromosome 10, and QTLs for Zn, As and Mn was on chromosome 12. The Zn concentration in F2 generation showed significant correlation with concentrations of As (r = -0.4), Cu (r = 0.5) and Fe (r = 0.2) (P < 0.01). Also, the Ca concentration was significantly related with Mn and Fe concentrations (P < 0.01). Fine mapping of these QTLs is underway to analyze their functional relationship.

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QTL Identification Using Combined Linkage and Linkage Disequilibrium Mapping for Milk Production Traits on BTA6 in Chinese Holstein Population

  • Hu, F.;Liu, J.F.;Zeng, Z.B.;Ding, X.D.;Yin, C.C.;Gong, Y.Z.;Zhang, Q.
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권10호
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    • pp.1261-1267
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    • 2010
  • Milk production traits are important economic traits for dairy cattle. The aim of the present study was to refine the position of previously detected quantitative trait loci (QTL) on bovine chromosome 6 affecting milk production traits in Chinese Holstein dairy cattle. A daughter design with 918 daughters from 8 elite sire families and 14 markers spanning the previously identified QTL region were used in the analysis. We employed a combined linkage and linkage disequilibrium analysis (LDLA) approach with two options for calculating the IBD probabilities, one was based on haplotypes of all 14 markers (named Method 1) and the other based on haplotypes with sliding windows of 5 markers (named Method 2). For milk fat yield, the two methods revealed a highly significant QTL located within a 6.5 cM interval (Method 1) and a 4.0 cM interval (Method 2), respectively. For milk protein yield, a highly significant QTL was detected within a 3.0 cM interval (Method 1) or a 2.5 cM interval (Method 2). These results confirmed the findings of our previous study and other studies, and greatly narrowed down the QTL positions.

수치임상도 제작을 위한 영상탑재 현장조사 시스템 개발 (Development of the Field Investigation System (FIS) loading Image Data for Digital Forest Type Mapping)

  • 유병오;권수덕;김성호
    • 한국산림과학회지
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    • 제97권4호
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    • pp.445-451
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    • 2008
  • 본 연구는 Tablet PC를 기반으로 정밀수치임상도 제작을 위한 현장조사용 맞춤형 시스템을 개발하는데 그 목적이 있다. 본 연구에서 개발한 FIS의 주요 개발내용 및 특징은 다음과 같다. FIS는 GPS에서 수신한 정보를 바탕으로 다양한 공간 Data에서 위치정보를 쉽게 파악할 수 있어 정보의 접근성이 용이한 장점이 있다. 또한 임상편집 뿐만 아니라 메모 및 야장도구를 통해 여러 가지 정보들을 현장에서 손쉽게 기록하고 관리할 수 있으며, 간단한 측정도구로 거리와 면적을 쉽게 산출할 수 있다. 본 연구에서 개발한 시스템을 활용하여 효율적인 현장조사가 가능하므로 현장에서 시간 및 비용을 절감할 수 있으며, 현장작업의 생산력이 향상될 것이다.

Gabor 웨이브렛과 FCM 군집화 알고리즘에 기반한 동적 연결모형에 의한 얼굴표정에서 특징점 추출 (Feature-Point Extraction by Dynamic Linking Model bas Wavelets and Fuzzy C-Means Clustering Algorithm)

  • 신영숙
    • 인지과학
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    • 제14권1호
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    • pp.10-10
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    • 2003
  • 본 논문은 Gabor 웨이브렛 변환을 이용하여 무표정을 포함한 표정영상에서 얼굴의 주요 요소들의 경계선을 추출한 후, FCM 군집화 알고리즘을 적용하여 무표정 영상에서 저차원의 대표적인 특징점을 추출한다. 무표정 영상의 특징점들은 표정영상의 특징점들을 추출하기 위한 템플릿으로 사용되어지며, 표정영상의 특징점 추출은 무표정 영상의 특징점과 동적 연결모형을 이용하여 개략적인 정합과 정밀한 정합 과정의 두단계로 이루어진다. 본 논문에서는 Gabor 웨이브렛과 FCM 군집화 알고리즘을 기반으로 동적 연결모형을 이용하여 표정영상에서 특징점들을 자동으로 추출할 수 있음을 제시한다. 본 연구결과는 자동 특징추출을 이용한 차원모형기반 얼굴 표정인식[1]에서 얼굴표정의 특징점을 자동으로 추출하는 데 적용되었다.

Deep Inferior Epigastric Perforators Topography for "Island Transverse Rectus Abdominis Musculocutaneous Flap" in Breast Reconstruction

  • Tae Hyun Kim;Seong Heum Jeong;Hee Chang Ahn
    • Archives of Plastic Surgery
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    • 제50권4호
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    • pp.354-360
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    • 2023
  • Background The Island transverse rectus abdominis musculocutaneous (TRAM) flap is well vascularized with very reliable blood flow, because all perforators of the zone I are included when it is harvested. The number of perforators, topographic mapping, and their relationship with reconstructed outcomes were investigated. Methods Fifty patients with Island TRAM breast reconstruction from September 2021 to August 2022 were investigated. The zone I was divided into a total of eight sections. Under the loupe magnification, all perforators larger than 0.5 mm in zone I were counted with fine dissection, and photographs were taken in background of vessel loops. Complications like flap necrosis, seroma, and hematoma were also investigated. Result There are 12 ideal perforators on average in zone I such as one perforator in section I, II, IV, V, VI, VIII, and three perforators in section III and VII. However, two perforators (M6 and L6) below arcuate line were sacrificed in the time of flap harvest to prevent hernia. Island TRAM included 10 perforators on average (5 perforators in each side) above arcuate line to be transferred to the recipient site. Only minor complications were identified. Conclusion The Island TRAM flap includes 10 perforators to get the vigorous blood flow. The periumbilical to upper medial perforators become more dominant in the perfusion of the flap after deep inferior epigastric artery division. Well preserved perforators will guarantee the satisfactory breast reconstruction with the least complication.