• Title/Summary/Keyword: Fine Mapping

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SSR Marker Linked to f Locus in Soybean

  • Nam, Ki-Chul;Kim, Myung-Sik;Jeong, Woo-Hyeun;Kim, Seok-Hyeon;Chung, Jong-Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.1
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    • pp.51-54
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    • 2007
  • Soybean has a morphological type with a broadened and flattened stem. Fasciation has been suggested as a new gene for soybean research. SSR marker linked to the $\Large f$ locus that controls fasciation phenotype has not identified within 10 cM. A mapping population consisting of 94 $F_2$ progenies was derived from a cross between wild type Clark (FF) and fasciation mutant C32 (${\Large f}{\Large f}$). The phenotype of $F_2$ individual plants was recorded at R2 and R3 growth stage from field. One-thousand 10-mer oligonucleotide RAPD primers and 29 SSR primers selected from the D1b+W of the soybean molecular linkage map were used. A genetic map was constructed from the segregating 35 RAPD, four SSR markers and one phenotypic(wild type/fasciation) marker. The segregation ratios of 3 : 1 observed in the $F_2$ population and the Chi-square values strongly suggest that the fasciation trait is controlled by a single recessive gene. Satt537 marker was linked to $\Large f$ locus at a distance of 9.6 cM. Assignment of the $\Large f$ locus to linkage group D1b+W and identification of markers can be used as an initial step for fine mapping of the $\Large f$ gene.

Characteristics of the Femto-second Pulsed Laser Ablation according to Feed Velocity on the Invar Alloy (펨토초 레이저의 이송속도에 따른 Invar 합금의 어블레이션 특성)

  • Chung, Il-Young;Kang, Kyung-Ho;Kim, Jae-Do;Sohn, Ik-Bu;Noh, Young-Chul;Lee, Jong-Min
    • Journal of the Korean Society for Precision Engineering
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    • v.26 no.3
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    • pp.25-31
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    • 2009
  • Femto-second laser ablation with the various feed velocities of the Invar alloy and the micro surface milling for the processing condition were studied. We used a regenerative amplified Ti:sapphire laser with a 1kHz repetition rate, 184fs pulse duration time and 785nm wavelength. Femto-second laser pulse was irradiated on the Invar alloy with the air blowing at the condition of various laser peak powers and feed velocities. An ablation characteristic according to feed velocity of the Invar alloy was appeared as the non-linear type at different zone of energy fluence. The micro surface milling of the Invar alloy using a mapping method was investigated. The optimal condition of micro surface milling was laser peak power of 22.8mW, feed velocity of 1 mm/s, beam gap of $1{\mu}m$. With the optimal processing condition, the fine rectangular shape without burr and thermal damage was achieved. Using the femto-second laser system, it demonstrates excellent tool for micro surface milling of the Invar alloy without heat effects and poor edge.

Development of a Web Service based GIS-Enabled Storm-surge Visualization System (웹 서비스 기반 GIS 연동 폭풍.해일 시각화 시스템 개발)

  • Kim, Jin-Ah;Park, Jin-Ah;Park, K.S.;Kwon, Jae-Il
    • Journal of KIISE:Computing Practices and Letters
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    • v.14 no.9
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    • pp.841-849
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    • 2008
  • Natural disaster such as inundation due to the typhoon induced storm-surge has inflicted severe losses on the coastal area. The problem of global warming and sea surface rising has issued and thus influences the increase of frequency and potential power of storm-surge. What we can do is to make intelligent effort to predict and prevent the losses through the early warning and prevention activity from the accurate prediction and forecasting about the time-varying storm-surge height and its arriving time resulted from the numerical simulation with sea observations. In this paper, we developed the web service based GIS-Enabled storm-surge visualization system to predict and prevent the storm-surge disasters. Moreover. for more accurate topography around coastal area and fine-grid storm-surge numerical model, we have accomplished GIS-based coastal mapping through LiDAR measurement.

Quantitative Trait Locus Mapping and Candidate Gene Analysis for Plant Architecture Traits Using Whole Genome Re-Sequencing in Rice

  • Lim, Jung-Hyun;Yang, Hyun-Jung;Jung, Ki-Hong;Yoo, Soo-Cheul;Paek, Nam-Chon
    • Molecules and Cells
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    • v.37 no.2
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    • pp.149-160
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    • 2014
  • Plant breeders have focused on improving plant architecture as an effective means to increase crop yield. Here, we identify the main-effect quantitative trait loci (QTLs) for plant shape-related traits in rice (Oryza sativa) and find candidate genes by applying whole genome re-sequencing of two parental cultivars using next-generation sequencing. To identify QTLs influencing plant shape, we analyzed six traits: plant height, tiller number, panicle diameter, panicle length, flag leaf length, and flag leaf width. We performed QTL analysis with 178 $F_7$ recombinant inbred lines (RILs) from a cross of japonica rice line 'SNU-SG1' and indica rice line 'Milyang23'. Using 131 molecular markers, including 28 insertion/deletion markers, we identified 11 main- and 16 minor-effect QTLs for the six traits with a threshold LOD value > 2.8. Our sequence analysis identified fifty-four candidate genes for the main-effect QTLs. By further comparison of coding sequences and meta-expression profiles between japonica and indica rice varieties, we finally chose 15 strong candidate genes for the 11 main-effect QTLs. Our study shows that the whole-genome sequence data substantially enhanced the efficiency of polymorphic marker development for QTL fine-mapping and the identification of possible candidate genes. This yields useful genetic resources for breeding high-yielding rice cultivars with improved plant architecture.

Quantitative Trait Loci Mapping for Porcine Backfat Thickness

  • Wu, X.L.;Lee, C.;Jiang, J.;Peng, Y.L.;Yan, H.F.;Yang, S.L.;Xiao, B.N.;Liu, X.C.;Shi, Q.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.7
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    • pp.932-937
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    • 2002
  • A partial genome scan using porcine microsatellites was carried out to detect quantitative trait loci (QTL) for backfat thickness (BFT) in a pig reference population. This population carried QTL on chromosomes 1, 13 and 18. The QTL on chromosome 1 was located between marker loci S0113 and SW1301. The QTL corresponded to very low density lipoprotein receptor gene (VLDLR) in location and in biological effects, suggesting that VLDLR might be a candidate gene. The QTL found on chromosome 13 was found between marker loci SWR1941 and SW864, but significance for the marker-trait association was inconsistent by using data with different generations. The QTL on chromosome 18 was discovered between markers S0062 and S0117, and it was in proximity of the regions where IGFBP3 and GHRHR were located. The porcine obese gene might be also a candidate gene for the QTL on chromosome 18. In order to understand genetic architecture of BFT better, fine mapping and positional comparative candidate gene analyses are necessary.

QTL analysis of for micronutrient content in rice grain

  • Lee, Hyun-Sook;Shim, Kyu-Chan;Jeon, Yun-A;Ahn, Sang-Nag
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.144-144
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    • 2017
  • Micronutrients such as zinc (Zn), iron (Fe), manganese (Mn) have important roles for development and growth in plants but it also have roles in animals and humans. In previous studies, a Korean weedy rice, KH2J was selected to have tolerance to heavy metal, lead (Pb) compared with a cultivar, Milyang23. To identify QTLs for micronutrients concentration in grain, an F2 population (120 plants) were developed from a cross between KH2J and an indica rice cultivar, Milyang23. To measure the concentration of eight ions, Zn, Fe, Mn, Pb, calcium (Ca), copper (Cu), cadmium (Cd) and arsenic (As), grains were collected and digested with 65% nitric acid, and the ion contents were measured using inductively coupled plasma mass spectrometry. A total 27 putative quantitative trait loci (QTLs) were detected on 12 chromosomes by single point analysis and 22 putative QTLs were detected by composite interval mapping. The co-locations of QTL for Zn, Fe and Mn were observed on chromosome 5. The QTLs for Cd, Cu and Zn were co-localized on chromosome 10, and QTLs for Zn, As and Mn was on chromosome 12. The Zn concentration in F2 generation showed significant correlation with concentrations of As (r = -0.4), Cu (r = 0.5) and Fe (r = 0.2) (P < 0.01). Also, the Ca concentration was significantly related with Mn and Fe concentrations (P < 0.01). Fine mapping of these QTLs is underway to analyze their functional relationship.

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QTL Identification Using Combined Linkage and Linkage Disequilibrium Mapping for Milk Production Traits on BTA6 in Chinese Holstein Population

  • Hu, F.;Liu, J.F.;Zeng, Z.B.;Ding, X.D.;Yin, C.C.;Gong, Y.Z.;Zhang, Q.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.10
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    • pp.1261-1267
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    • 2010
  • Milk production traits are important economic traits for dairy cattle. The aim of the present study was to refine the position of previously detected quantitative trait loci (QTL) on bovine chromosome 6 affecting milk production traits in Chinese Holstein dairy cattle. A daughter design with 918 daughters from 8 elite sire families and 14 markers spanning the previously identified QTL region were used in the analysis. We employed a combined linkage and linkage disequilibrium analysis (LDLA) approach with two options for calculating the IBD probabilities, one was based on haplotypes of all 14 markers (named Method 1) and the other based on haplotypes with sliding windows of 5 markers (named Method 2). For milk fat yield, the two methods revealed a highly significant QTL located within a 6.5 cM interval (Method 1) and a 4.0 cM interval (Method 2), respectively. For milk protein yield, a highly significant QTL was detected within a 3.0 cM interval (Method 1) or a 2.5 cM interval (Method 2). These results confirmed the findings of our previous study and other studies, and greatly narrowed down the QTL positions.

Development of the Field Investigation System (FIS) loading Image Data for Digital Forest Type Mapping (수치임상도 제작을 위한 영상탑재 현장조사 시스템 개발)

  • Yoo, Byungoh;Kwon, Sudeok;Kim, Sungho
    • Journal of Korean Society of Forest Science
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    • v.97 no.4
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    • pp.445-451
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    • 2008
  • This study was carried out to develop Tablet PC based customizing system for fine mapping of forest cover type. The major contents and characteristics of FIS developed in this study were as follows. Field Investigation System (FIS) has a merit of accessibility to display exact location in various spatial data with position information received from the GPS. FIS can be used to record and manage many field information on which field investigation is done, with the help of the memo tool, field-sheet tool, calculating distance and area with measuring tool as well as editing forest type. It is possible to do field investigation effectively using FIS developed in this study. Accordingly, investigation and time costs can be reduced and field-work productivity will be improved.

Feature-Point Extraction by Dynamic Linking Model bas Wavelets and Fuzzy C-Means Clustering Algorithm (Gabor 웨이브렛과 FCM 군집화 알고리즘에 기반한 동적 연결모형에 의한 얼굴표정에서 특징점 추출)

  • Sin, Yeong Suk
    • Korean Journal of Cognitive Science
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    • v.14 no.1
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    • pp.10-10
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    • 2003
  • This paper extracts the edge of main components of face with Gabor wavelets transformation in facial expression images. FCM(Fuzzy C-Means) clustering algorithm then extracts the representative feature points of low dimensionality from the edge extracted in neutral face. The feature-points of the neutral face is used as a template to extract the feature-points of facial expression images. To match point to Point feature points on an expression face against each feature point on a neutral face, it consists of two steps using a dynamic linking model, which are called the coarse mapping and the fine mapping. This paper presents an automatic extraction of feature-points by dynamic linking model based on Gabor wavelets and fuzzy C-means(FCM) algorithm. The result of this study was applied to extract features automatically in facial expression recognition based on dimension[1].

Deep Inferior Epigastric Perforators Topography for "Island Transverse Rectus Abdominis Musculocutaneous Flap" in Breast Reconstruction

  • Tae Hyun Kim;Seong Heum Jeong;Hee Chang Ahn
    • Archives of Plastic Surgery
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    • v.50 no.4
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    • pp.354-360
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    • 2023
  • Background The Island transverse rectus abdominis musculocutaneous (TRAM) flap is well vascularized with very reliable blood flow, because all perforators of the zone I are included when it is harvested. The number of perforators, topographic mapping, and their relationship with reconstructed outcomes were investigated. Methods Fifty patients with Island TRAM breast reconstruction from September 2021 to August 2022 were investigated. The zone I was divided into a total of eight sections. Under the loupe magnification, all perforators larger than 0.5 mm in zone I were counted with fine dissection, and photographs were taken in background of vessel loops. Complications like flap necrosis, seroma, and hematoma were also investigated. Result There are 12 ideal perforators on average in zone I such as one perforator in section I, II, IV, V, VI, VIII, and three perforators in section III and VII. However, two perforators (M6 and L6) below arcuate line were sacrificed in the time of flap harvest to prevent hernia. Island TRAM included 10 perforators on average (5 perforators in each side) above arcuate line to be transferred to the recipient site. Only minor complications were identified. Conclusion The Island TRAM flap includes 10 perforators to get the vigorous blood flow. The periumbilical to upper medial perforators become more dominant in the perfusion of the flap after deep inferior epigastric artery division. Well preserved perforators will guarantee the satisfactory breast reconstruction with the least complication.