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Improving Corsican pine somatic embryo maturation: comparison of somatic and zygotic embryo morphology and germination

  • Wtpsk, Senarath;Shaw, D.S.;Lee, Kui-Jae;Lee, Wang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.04a
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    • pp.61-62
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    • 2003
  • Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve raw material uniformity and quality. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Several factors limit commercialization of SE for Corsican pine, including low initiation rates, low culture survival, culture decline causing low or no embryo production, and inability of somatic embryos to fully mature, resulting in low germination and reduced vigour of somatic seedlings. The objective was to develop a Corsican pine maturation medium that would produce cotyledonary embryos capable of germination. Treatments were arranged in a completely randomized design. Data were analyzed by analysis of variance, and significant differences between treatments determined by multiple range test at P=0.05. Corsican pine (Pinus nigra var. maritima) cultures were initiated on modified !P6 medium. Modifications of the same media were used for culture multiplication and maintenance. Embryogenic cultures were maintained on the same medium semi solidified with 2.5 g/l Gelrite. A maturation medium, capable of promoting the development of Corsican pine somatic embryos that can germinate, is a combination of iP6 modified salts, 2% maltose, 13% polyethylene glycol (PEG), 5 mg!l abscisic acid (ABA), and 2.5 g/l Gelrite. After initiation and once enough tissue developed they were grown in liquid medium. Embryogenic cell suspensions were established by adding 0.951.05 g of 10- to 14-day-old semisolid-grown embryogenic tissue to 9 ml of liquid maintenance media in a 250ml Erlenmeyer flask. Cultures were then incubated in the dark at 2022$^{\circ}$C and rotated at 120 rpm. After 2.53 months on maturation medium, somatic embryos were selected that exhibited normal embryo shape. Ten embryos were placed horizontally on 20 ml of either germination medium ($\frac{2}{1}$strength Murashige and Skoog (1962) salts with 2.5 g/l activated charcoal) or same medium with copper sulphate adjusted to 0.25 mg/1 to compensate for copper adsorption by activated carbon. 2% and 4% maltose was substituted by 7.5% and 13% PEG respectively to improve the yield of the embryos. Substitution of' maltose with PEG was clearly beneficial to embryo development. When 2% of the maltose was replaced with 7.5% PEG, many embryos developed to large bullet-shaped embryos. At latter stages of development most embryos callused and stopped development. A few short, barrel-shaped cotyledonary embryos formed that were covered by callus on the sides and base. When 4% of the maltose was removed and substituted with 13% PEG, the embryos developed further, emerging from the callus and increasing yield slightly. Microscopic examination of the cultures showed differing morphologies, varying from mostly single cells or clumps to well-formed somatic embryos that resembled early zygotic embryos only liquid cultures with organized early-stag. A procedure for converting and acclimating germinants to growth in soil and greenhouse conditions is also tested. Seedling conversion and growth were highly related to the quality of the germinant at the time of planting. Germinants with larger shoots, longer, straighter hypocotyls and longer roots performed best. When mature zygotic embryos germinate the root emerges, before or coincident with the shoot. In contrast, somatic embryos germinate in reverse sequence, with the cotyledons greening first, then shoot emergence and then, much later, if at all, the appearance of the root. Somatic seedlings, produced from the maturation medium, showed 100% survival when planted in a field setting. Somatic seedlings showed normal yearly growth relative to standard seedlings from natural seed.

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Teaching Behavior, Confidence and Satisfaction among Teachers who Solo-Teach Technology and Home Economics in High School (고등학교 "기술.가정" 교과 교사의 교수행동, 교수자신감, 교수만족도)

  • Park Hyun-Jin;Shin Hye-Won
    • Journal of Korean Home Economics Education Association
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    • v.18 no.2 s.40
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    • pp.111-123
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    • 2006
  • This study was conducted on teachers who currently are teaching 'Technology and Home Economics' in high school, and teaching it alone, as opposed to team teaching. The study examined the teachers' fields of major study relative to their teaching behaviors and levels of confidence and satisfaction while teaching this course. The study also compared teaching behaviors, confidence and satisfaction among teachers who either have or have not participated in training sessions specifically-targeting teachers who are lack of relevant knowledge and skills in a major field. Questionnaires were mailed to high school teachers who were solo-teaching 'Technology and Home Economics' at high schools in Gyeonggi Do. Data from 83 respondents were used for the final analysis. The findings were as follows: First, it was determined that teachers tend to be more active when teaching subjects in which they have majored than subjects in which they have not. Teachers who have majored in home economics were most active when teaching home economics, followed by technology major teachers teaching technology, home economics major teachers teaching technology and technology major teachers teaching home economics, the last group exhibiting the least willingness to teach. Similarly, all teachers exhibited less confidence in areas not relevant to their majors, and confidence especially was lower while teaching practical skills versus theories. Teachers also were found to be less satisfied with teaching subjects outside of their majors. Second, analyzing technology majors currently teaching home economics and comparing those who have and have not participated in training sessions for home economics teaching, we found that 1) technology majors with such training feel that the training experience helped them in preparing various teaching media and in their evaluation of students' understanding of basic concepts and practices; 2) these training programs increased teachers' confidence teaching theories, but not practical skills; and 3) after they had participated in training programs, teachers' satisfaction increased in terms of producing teaching agendas and selecting textbooks and teaching media for their classes. However, training programs were found to have no effect on home economics majors who had participated in technology training programs, in terms of teaching behaviors, confidence or satisfaction teaching technology.

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Study on the Growth and the Yield of Ecotype of Garlics in Main Producing Districts in Korea (주산단지(主産團地) 마늘의 생태형(生態型)에 따른 생장(生長)과 수량(收量)에 관(關)한 연구(硏究))

  • Ra, Woo-Hyun;Park, Kuen-Woo
    • Korean Journal of Environmental Agriculture
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    • v.6 no.1
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    • pp.67-75
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    • 1987
  • The major objectives of this study were to find out the growth and yield of two ecotypes of Korean garlic in main garlic producing districts in Korea. The data were collected by the field survey which had been conducted at 270 township in 58 major garlic production countries throughout the country on 10th, 20th, and 30th day of every month from 1982 to 1984. The results of this study were as follow: 1) Sowing period of garlic of southern and northern ecotype were around September 20 and October 20, respectively. 2) Average number of plants per $3.3m^2$ of the southern and northern ecotype were 123 and 100, respectively. 3) Leaf emergence time of southern ecotype was before the beginning of winter (November) and that of northern ecotype was from early February till April. 4) Stepwise multiple regression analysis showed that the plant heights measured on November 30 for southern ecotype and on June 30 for northern ecotype most adequately predicted the yield of garlic. The relationship between yield and plant height were as follows: Southern ecotype; Y=571.56+5.34X Northern ecotype; Y=251.81+5.45X where Y is yield expressed in Kg/l0a and X is height in cm at the respective date. 5) The number of leaves increased until harvest in both ecotypes. At harvest, the number of leaves in southern and northern ecotype were 10 and 8, respectively. Number of leaves counted on January 20 for southern ecotype and on June 20 for northern ecotype correlated best with the yield of the ecotypes. 6) The highest senescent portion of southern ecotype and northern ecotype were seen on January 30 and May 30, respectively. Stepwise multiple regression analysis showed that the senescent portion of southern ecotype counted on January 30 and that of northern ecotype on June 20 mostly affected the yield. 7) Average yield of southern and northern ecotype at the main garlic producing districts were 771 and 652Kg/10a, respectively.

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A Basic Study on the Euryale ferox Salisbury for Introduction in Garden Pond(II) - Focusing with Soil and Water Conditions - (정원 연못내 가시연꽃(Euryale ferox Salisbury) 도입을 위한 기초연구 II - 토양과 수환경을 중심으로 -)

  • Lee, Suk-Woo;Rho, Jae-Hyun;Park, Jae-Cheol;Kim, Hwa-Ok
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.34 no.3
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    • pp.28-37
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    • 2016
  • Through the research and analysis on the hydrological environment and soil environment of habitats through documentary and field studies over 14 habitats of Euryale ferox Salisb. within Jeollabukdo, with the objective of acquiring the basic data for forming an environment based on plantation of reservoirs that are composed with Euryale ferox, the following results were obtained. As a result of analyzing the construction period of the habitats of Euryale ferox from a total of 14 places, the average period of duration after construction of all subject reservoirs appeared to be 71.8 years. Moreover, when examining the relationship between the age of reservoirs and eutrophication, it could be judged that at least the eutrophication of subsoil and water environment is not an obstacle to the growth of Euryale ferox grows in habitats that have a reservoir age of approximately 70 years or more. As a result of analyzing the gardening of soil sediment of the Euryale ferox habitats, the component ingredients appeared to be composed of 80.2% of clay, 16.7% of silt and 3.1% of sand, and the soil class pursuant to such was classified as 'heavy clay'. The organic matter contents of soil sediment appeared to be an average of 36g/kg, and there appeared to be no noticeable difference between the habitats and non-habitats of Euryale ferox. The water quality environment of Euryale ferox habitat appeared to be pH 6.5~7.9, concentration of dissolved oxygen to be $1.8{\sim}8.8mg/{\ell}$, concentration of COD to be $6.8{\sim}74mg/{\ell}$, floating materials to be $2.0{\sim}213mg/{\ell}$, total nitrogen to be $0.422{\sim}10.723mg/{\ell}$, and phosphate to be $0.003{\sim}0.126mg/{\ell}$. The average DO concentration of Aedang Reservoir at Jeongeup, Daejeong Reservoir at Imsil, and Myeongdeokji at Gimje with high vitality and green coverage ratio of Euryale ferox appeared to be $3.5mg/{\ell}$, total nitrogen to be $1.33mg/{\ell}$, and concentration of phosphorus-phosphate to be $0.061mg/{\ell}$. When comparing such with the entire average value, the DO and total nitrogen concentration appeared to be rather low, and the phosphorus-phosphate concentration appeared to be higher by two times or more, thus, an in-depth study on the correlation of the vitality of Euryale ferox Salisb. and concentration of phosphorate-phosphorus will be needed in the future.

Synoptic Climatological Characteristics of Spring Droughts in Korea (한국의 춘계한발의 종관기후학적 특성)

  • Yang, Jin-Suk
    • Journal of the Korean association of regional geographers
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    • v.4 no.1
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    • pp.43-56
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    • 1998
  • The purpose of this study is to identify distributional characteristics of climatic elements and to analyze synoptic characteristics on the pressure fields for spring droughts in Korea. In the distributions of minimum temperature during the spring droughts, positive anomalies and negative anomalies are mixed up, but in March the negative anomaly areas are widely distributed in Korea. It implies that the droughts of March have more frequent occurrences of the west-high, east-low pressure patterns. In the maximum air temperatures, the positive anomalies appear in Korea. It indicates that the spring droughts have rain days, cloud amount and humidities less than normal. As a result, the amount of evaporation is increased in Korea. In the pressure anomaly of surface pressure fields, the positive anomalies appear in the west, negative anomalies in the east in March, but in May the positive anomalies appeared zonally around the Korean peninsula. It indicates that March droughts have more frequent occurrences of the west-high. east-low patterns, but in May the Korean Peninsula has more frequent recurrences of the migratory anticyclone patterns. The height anomaly patterns of 500hPa pressure surface in spring droughts are similarly shown to those of surface fields. In March droughts, the positive height anomalies appear in the west, the negative height anomalies in the east, but in April the negative height anomaly areas are extended to the west part. In May the positive anomalies appear zonally around the Korean Peninsula, and strong positive height anomalies appear around the Kamchatka Peninsula and the sea of Okhotsk. These are the result of circulations that inhibit the eastward movement of westerlies and that has persistent anticyclone circulation patterns around the Korean Peninsula. As a result, the zonal indices of westerlies during March and April droughts are lower than normal, but higher in May. These data indicate that early spring droughts are associated with weak zonal flow, but the late spring droughts are obviously related with strong zonal flow. In addition, during early spring droughts the abnormally deep trough over the west coast of the North Pacific Ocean that accompanied the anticyclone was associated with frequent advection of air from the dry regions in the Central Asia into the Korean Peninsula. The atmospheric circulation patterns at the height of the 500hPa pressure surface in May was quite different from March and April circulation patterns. Instead of the abnormal ridge in the west and trough in the east, the circulation pattern in May was characterized by a much stronger than normal anticyclone over the Korean Peninsula. Also, the zonal indices of westerlies in May are higher than normal. The occurrences of drought in early spring, therefore, have mechanism different from those of late spring.

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Identification of Advanced Argillic-altered Rocks of the Haenam Area, Using by ASTER Spectral Analysis (ASTER 분광분석을 통한 해남지역 강고령토변질 암석의 식별)

  • Lee, Hong-Jin;Kim, Eui-Jun;Moon, Dong-Hyeok
    • Economic and Environmental Geology
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    • v.44 no.6
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    • pp.463-474
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    • 2011
  • The Haenam epithermal mineralized zone is located in the southwestern part of South Korea, and hosts low sulfidation epithermal Au-Ag deposit (Eunsan-Moisan) and clay quarries (Okmaesan, Seongsan, and Chunsan). Epithermal deposits and accompanying hydrothermal alteration related to Cretaceous volcanism caused large zoned assemblages of hydrothermal alteration minerals. Advanced argillic-altered rocks with mineral assemblages of alunite-quartz, alunite-dickite-quartz, and dickite-kaolinite-quartz exposed on the Okmaesan, Seongsan, and Chunsan area. Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), with three visible and near infrared bands, six shortwave infrared bands, and five thermal infrared bands, was used to identify advanced argillic-altered rocks within the Haenam epithermal mineralized zone. The distinct spectral features of hydrothermal minerals allow discrimination of advanced argillic-altered rocks from non-altered rocks within the study area. Because alunite, dickite, and kaolinite, consisting of advanced argillic-altered rocks within the study area are characterized by Al-O-H-bearing minerals, these acid hydrothermal minerals have a strong absorption feature at $2.20{\mu}m$. The band combination and band ratio transformation cause increasing differences of DN values between advanced argillic-altered rock and non-altered rock. The alunite and dickite-kaolinite of advanced argillic-altered rocks from the Okmaesan, Seongsan, and Chunsan have average DN values of 1.523 and 1.737, respectively. These values are much higher than those (1.211 and 1.308, respectively) of non-altered area. ASTER images can remotely provide the distribution of hydrothermal minerals on the surface. In this way good relation between ASTER spectra analysis and field data suggests that ASTER spectral analysis can be useful tool in the initial steps of mineral exploration.

A study on application of fractal structure on graphic design (그래픽 디자인에 있어서 프랙탈 구조의 활용 가능성 연구)

  • Moon, Chul
    • Archives of design research
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    • v.17 no.1
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    • pp.211-220
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    • 2004
  • The Chaos theory of complexity and Fractal theory which became a prominent figure as a new paradigm of natural science should be understood not as whole, and not into separate elements of nature. Fractal Dimensions are used to measure the complexity of objects. We now have ways of measuring things that were traditionally meaningless or impossible to measure. They are capable of describing many irregularly shaped objects including man and nature. It is compatible method of application to express complexity of nature in the dimension of non-fixed number by placing our point of view to lean toward non-linear, diverse, endless time, and complexity when we look at our world. Fractal Dimension allows us to measure the complexity of an object. Having a wide application of fractal geometry and Chaos theory to the art field is the territory of imagination where art and science encounter each other and yet there has not been much research in this area. The formative word has been extracted in this study by analyzing objective data to grasp formative principle and geometric characteristic of (this)distinct figures of Fractals. With this form of research, it is not so much about fractal in mathematics, but the concept of self-similarity and recursiveness, randomness, devices expressed from unspeakable space, and the formative similarity to graphic design are focused in this study. The fractal figures have characteristics in which the structure doesn't change the nature of things of the figure even in the process if repeated infinitely many times, the limit of the process produces is fractal. Almost all fractals are at least partially self-similar. This means that a part of the fractal is identical to the entire fractal itself even if there is an enlargement to infinitesimal. This means any part has all the information to recompose as whole. Based on this scene, the research is intended to examine possibility of analysis of fractals in geometric characteristics in plasticity toward forms in graphic design. As a result, a beautiful proportion appears in graphic design with calculation of mathematic. It should be an appropriate equation to express nature since the fractal dimension allows us to measure the complexity of an object and the Fractla geometry should pick out high addition in value of peculiarity and characteristics in the complex of art and science. At the stage where the necessity of accepting this demand and adapting ourselves to the change is gathering strength is very significant in this research.

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Evaluation of Adsorbent Sampling Methods for Volatile Organic Compounds in Indoor and Outdoor Air (실내·외 공기 중 휘발성 유기화합물에 대한 흡착 시료채취 방법의 평가)

  • Baek, Sung-Ok;Moon, Young-Hun
    • Analytical Science and Technology
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    • v.17 no.6
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    • pp.496-513
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    • 2004
  • This study was carried out to evaluate the performance of sampling and analytical methodology used for the measurement of toxic volatile organic compounds (VOCs) in the ambient air. VOCs were determined by the adsorbent tube sampling and automatic thermal desorption coupled with GC/MSD analysis. Target analytes were 33 compounds including major aromatic compounds such as BTEX, and halogenated compounds. The methodology was investigated with a wide range of different adsorbents which are commercially available and have been frequently adopted for the VOC measurement. A total of 10 adsorbents were tested in this study: 6 carbon-based adsorbents such as Carbotrap, Carbopack B, Carbosieve S-III, Carboxen 1000, Carbotrap C, Activated Charcoal; and 4 polymer-based adsorbents including Tenax, Porapak Q, Chromosorb 102, and Chromosorb 106. The sampling performance was evaluated with respect to the sampling capacity of VOCs with single-adsorbent and multiple-adsorbents methods for standard samples and field samples. As a result, the best adsorbents for single-adsorbent method in the sampling of toxic organic compounds (including benzene, toluene, xylenes etc.) appeared to be Carbotrap, Carbopack B and Tenax TA. On the other hand, Chromosorb 102, Chromosorb 106 and Porapak Q were found to be unsuitable adsorbents for VOC measurement based on thermal desorption method. Multi-adsorbent packings were evaluated with 4 carbon-based adsorbents, which classified by 3 combination sets of double adsorbents and 2 combination sets of triple adsorbents. The results indicated that the most suitable combination for toixc VOC measurements is Carbotrap C with Carbotrap. Multi-sorbents tubes packed with a strong adsorbent such as Carbosieve S-III or Carboxen 1000 were found to be relatively unsuitable for several compounds, not only owing to the effect of migration of adsorbed compounds from weaker adsorbent to stronger adsorbent, but to hydrophobic nature of the adsorbents. Therefore, it should be addressed that selection of a proper adsorbent (or combination of multi sorbents) is extremely important to obtain reliable data for the concentrations of toxic VOCs in indoor and outdoor environments.

A SURVEY ON THE USE OF COMPOSITE RESIN IN CLASS II RESTORATION IN KOREA (2급 와동 수복 시 한국 치과 지사들의 복합레진 사용 실태 연구)

  • Shin, Dong-Ho;Park, Se-Eun;Yang, In-Seok;Chang, Ju-Hea;Lee, In-Bog;Cho, Byeong-Hoon;Son, Ho-Hyun
    • Restorative Dentistry and Endodontics
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    • v.34 no.2
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    • pp.87-94
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    • 2009
  • The purpose of this study was to assess the current materials, methods and difficulties according to the year of licence and educational background of Korean dentists in Class II direct composite resin restorations. Total 17 questions were included in the questionnaire. Questions were broadly divided into two parts: first. operator's information. and second. the materials and methods used in Class II posterior composite restoration. The questionnaire was sent to dentists enrolled in Korean Dental Association via e-mail. Total 12,193 e-mails were distributed to dentists. 2,612 e-mails were opened, and 840 mails (32.2%) were received from respondents. The data was statically analyzed by chi-square test using SPSS(v. 12.0.1, SPSS Inc. Chicago, IL, USA). Male dentists among respondents was 79%. 60.3% of the respondents acquired their licences recently (1998-2007), and 77% practiced in private offices. 83.4% have acquired their knowledge through school lectures, conferences and seminars. For the Class II restorations, gold inlays were preferred by 65.7% of respondents, while direct composite resin restorations were used by 12.1 % amalgam users were only 4.4% of respondents. For the restorative technique, 74.4% of respondents didn't use rubber dam as needed. For the matrix. mylar strip (53.4%), metal matrix (33.8%) and Palodent system (6.5%) were used. 99.6% of respondents restored the Class II cavity by incremental layering. Obtaining of the tight interproximal contact was considered as the most difficult procedure (57.2%) followed by field isolation (21 %). Among various bonding systems, 22.6% of respondents preferred SE Bond and 20.2% used Single Bond. Z-250 was used most frequently among a variety of composite resins.

Simulation of Detailed Wind Flow over a Locally Heated Mountain Area Using a Computational Fluid Dynamics Model, CFD_NIMR_SNU - a fire case at Mt. Hwawang - (계산유체역학모형 CFD_NIMR_SNU를 이용한 국지적으로 가열된 산악지역의 상세 바람 흐름 모사 - 화왕산 산불 사례 -)

  • Koo, Hae-Jung;Choi, Young-Jean;Kim, Kyu-Rang;Byon, Jae-Young
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.11 no.4
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    • pp.192-205
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    • 2009
  • The unexpected wind over the Mt. Hwawang on 9 February 2009 was deadly when many spectators were watching a traditional event to burn dried grasses and the fire went out of control due to the wind. We analyzed the fatal wind based on wind flow simulations over a digitized complex terrain of the mountain with a localized heating area using a three dimensional computational fluid dynamics model, CFD_NIMR_SNU (Computational Fluid Dynamics_National Institute of Meteorological Research_Seoul National University). Three levels of fire intensity were simulated: no fire, $300^{\circ}C$ and $600^{\circ}C$ of surface temperature at the site on fire. The surface heat accelerated vertical wind speed by as much as $0.7\;m\;s^{-1}$ (for $300^{\circ}C$) and $1.1\;m\;s^{-1}$ (for $600^{\circ}C$) at the center of the fire. Turbulent kinetic energy was increased by the heat itself and by the increased mechanical force, which in turn was generated by the thermal convection. The heating together with the complex terrain and strong boundary wind induced the unexpected high wind conditions with turbulence at the mountain. The CFD_NIMR_SNU model provided valuable analysis data to understand the consequences of the fatal mountain fire. It is suggested that the place of fire was calm at the time of the fire setting due to the elevated terrain of the windward side. The suppression of wind was easily reversed when there was fire, which caused updraft of hot air by the fire and the strong boundary wind. The strong boundary wind in conjunction with the fire event caused the strong turbulence, resulting in many fire casualties. The model can be utilized in turbulence forecasting over a small area due to surface fire in conjunction with a mesoscale weather model to help fire prevention at the field.