• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.56-56
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• 2003

Using gel filtration chromatography (GFC), pure separation of high molecular silk fibroin was obtained and silk fibroin microsphere particles (SFMP) could be simply made by spray dryer method. Also, some of its physicochemical properties and morphology were investigated. The average molecular weight (Mw) of pure silk fibroin protein dissolved in calcium chloride is about 61, 500 g/mol as measured by gel permeation chromatography(GPC). (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.2
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• pp.151-155
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• 2003

Using gel filtration chromatography, high molecular silk fibroin with high purity was obtained and silk flbroin microsphere particles (SFMP) could be simply made by spray dryer method. Also, some of the physicochemical properties of SFMP and morphology were investigated. The average molecular weight of pure silk fibroin protein dissolved in calcium chloride is about 61,500g/㏖ as measured by gel permeation chromatography. SFMP was spherical in shape, and particles, sized average of 2 ${\pm}$ 10 ${\mu}$, were observed by SEM and particle analyzer, respectively. Obtaining microspheres particles by spray dryer method accelerated the transition from the random coil to the $\beta$-sheet structure during spray dryer treatment. It was identified by the basic fourier transform infrared spectroscopy of SFMP. The swelling ratio of SFMP is majorly dependent on the pH of the solution, not on the occurred gelation. The characteristic structure, which might be applicable to immobilization of drugs is superior to other matrix materials for the use of biomaterials with skin affinity.

• KSBB Journal
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• v.29 no.5
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• pp.328-335
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• 2014

Vehicle toxicity is one of the main obstacles for intracellular delivery of bioactive compounds. Silk fibroin is a natural polymer proven to have high biocompatibility since being used as suture material. In this report, fibroin microspheres were prepared without any chemical modification or cross-linking not to affect its biocompatibility. The microspheres were taken up by more than 90% of 3T3 cells. Cellular uptake continued after medium replenishment with a different-colored fluorescent microsphere, suggesting that simultaneous ingestion and exocytosis occurred. Cellular uptake of fibroin microspheres did not affect cell viability. Intracellular trafficking of the microspheres using lysosome-specific fluorescent dye revealed that fibroin microspheres were localized both in the cytoplasm and in the lysosome. Accordingly, fibroin microspheres can be a potential vehicle for intracytoplasmic delivery of large cargos, such as mixtures of proteins, nutrients or artificial organelles.

• Applied Chemistry for Engineering
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• v.34 no.2
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• pp.186-191
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• 2023

The goal of this study was to use gelatin to modify the surface of fibroin microspheres to enhance their biofunctionality for tissue engineering and regenerative medicine applications. Three different methods were used for the modification: coating, incorporation, and covalent bonding. Wound-healing assays demonstrated that gelatin modification of fibroin microspheres enhances 3T3 and HDF cell migration. Although the level of gelatin coverage varied depending on the method used, there was no significant difference between the modified microspheres. The gelatin-modified microspheres also increased the migration velocity of individual 3T3 cells. The results suggest that gelatin modification of fibroin microspheres is a promising approach for developing functional biomaterials with enhanced biological properties. Further optimization of gelatin modification is necessary to maximize the biofunctionality of fibroin microspheres.

• Polymer(Korea)
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• v.34 no.4
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• pp.321-325
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• 2010

Fibroin is a biopolymer available in large quantity from silk fiber and has a long history of use as a suture proving biocompatibility. In this report, fibroin microspheres has been fabricated for biomaterial applications. W/O emulsion of regenerated fibroin droplets in a continuous phase of decane with mixed surfactants was dried to facilitate fibroin gelation and the condensed fibroin microspheres were harvested. The ratio of mixed surfactants and their proportions to decane were determined to prepare a stable W/O emulsion. A spherical form of fibroin gels was obtained from the W/O emulsion agitated at 600 rpm. Scanning electron microscopy revealed that number average sizes of the fibroin microspheres were 21.6 and 8.5 ${\mu}m$ when dried under ambient conditions or under vacuum, respectively. Tomography of the spheres revealed that their internal structures are packed or hollowed. Hollow and hemispherical forms of microspheres were also prepared by using porogen.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2001.10a
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• pp.49-49
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• 2001

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