• Title/Summary/Keyword: Fiber Ratio

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Enzymatic Hydrolysis of Yellowfin Sole Skin Gelatin in a Continuous Hollow Fiber Membrane Reactor (연속식 중공사막 반응기를 이용한 각시가자미피 젤라틴의 가수분해)

  • KIM Se-Kwon;BYUN Hee-Guk;KANG Tae-Jung;SONG Dae-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.2
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    • pp.120-132
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    • 1993
  • A continuous hollow fiber membrane reactor(CHFMR) was developed and optimized for the production of yellowfin sole(Limanda aspera) skin gelatin hydrolysates using trypsin. The results were summerized as follows: The $K_m$ value of the CHFMR was 2.4 times higher than that of the batch reactor, indicating reduced enzyme affinity for the substrate. The $K_2$ value of the CHFMR was 8.5 times lower than that of the batch process, showing a significant reduction in trypsin activity in the CHFMR. The optimum operating conditions for the CHFMR process were $55^{\circ}C$, pH 9.0, flux 7.79 ml/min, residence time 77min, and trypsin to substrate ratio, 0.01(w/w) After operating for 60min under the above conditions, $79\%$ of the total amount of initial gelatin was hydrolysed. Enzyme leakage was observed through the 10,000 MWCO membrane after the 20min of reactor operation, while none occurred after 5hr. Total enzyme leakage was about $12.95\%$ at $55^{\circ}C$ for 5hrs. However, there was no apparent correlation between enzyme leakage and substrate hydrolysis. The membrane has a significant effect on trypsin activity loss for 60min of the CHFMR operation. The CHFMR operating with the membrane lost $34\%$ of the initial activity versus a $23\%$ loss of activity after 3hr in the continuous reactor lacking the hollow fiber membrane. The measurement of fouling property showed that relative flux reduction was $91\%$ and flux recover rate was $92\%$ at $10\%$ substrate solution. The productivity(378.85mg product/mg enzyme) of the CHFMR was more than 4 times higher than that of the batch reactor at $55^{\circ}C$.

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Anti-Obesity Effect of Red Garlic Composites in Rats Fed a High Fat-Cholesterol Diet (고지방-콜레스테롤 식이성 흰쥐에서 홍마늘 복합물의 항비만 효과)

  • Lee, Soo-Jung;Hwang, Cho-Rong;Kang, Jae-Ran;Shin, Jung-Hye;Kang, Min-Jung;Sung, Nak-Ju
    • Journal of Life Science
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    • v.22 no.5
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    • pp.671-680
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    • 2012
  • Three kinds of dietary composites-R+T, R+F, and R+TF-were combined in green tea (T), dietary fiber (F), and green tea dietary fiber mixture (TF) to red garlic extract (RG), respectively. The effects of their diets on anti-obesity were investigated $in$ $vitro$ and $in$ $vitro$ in obese rats induced high fat-cholesterol. In $in$ $vivo$ rats, the total phenolic content of the R+T and R+TF was 1.9~2.0 times higher, and their total cholesterol adsorption was 9.5~11.5 times higher than that of RG. $In$ $vivo$, male Sprague-Dawley rats were divided into 6 groups (Normal, HFC, HRG, HR+T, HR+F and HR+TF). Afterwards, the diets of the HRG, HR+T, HR+F, and HR+TF groups were supplemented with 1% of RG and its dietary composites (R+T, R+F, and R+TF) for 4 weeks, respectively. The final body weight of the HRG, HR+T, HR+F, and HR+TF groups decreased significantly compared to the group fed high fat-cholesterol (HFC), but the food efficiency ratio was not significantly different from the HFC group. The liver weight of the HFC group doubled compared to the normal group, whereas that of HR+T and HR+TF groups decreased significantly. The weight of visceral and epididymal fat decreased significantly in the groups fed the composites compared to the HFC group. The obesity index of HR+TF group decreased significantly only when compared to the HFC group. The serum lipid profile such as total lipids, cholesterol, triglyceride, LDL- and VLDL-cholesterol, as well as the atherogenic index and cardiac risk factors decreased drastically in all experimental groups compared to the HFC group, and the levels of HR+T, HR+F and HR+TF groups were a similar trend. GPT activity was not significantly different among the groups fed the composites, and it decreased significantly in the HRG group. The content of the lipid peroxide level decreased significantly in the HRG group and in the groups fed the composites, compared to the HFC group. Serum antioxidant activity was the highest in the HR+T group. We suggest that the hypolipidemic and anti-obesity effect of the RG composites, achieved by mixing green tea extract and/or dietary fiber, was due to their total phenolic content and total cholesterol adsorption effect.

Behavior of Steel Fiber-Reinforced Concrete Exterior Connections under Cyclic Loads (반복하중을 받는 강섬유 보강 철근콘크리트 외부 접합부의 거동 특성)

  • Kwon, Woo-Hyun;Kim, Woo-Suk;Kang, Thomas H.K.;Hong, Sung-Gul;Kwak, Yoon-Keun
    • Journal of the Korea Concrete Institute
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    • v.23 no.6
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    • pp.711-722
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    • 2011
  • Beam-column gravity or Intermediate Moment frames subjected to unexpected large displacements are vulnerable when no seismic details are provided, which is typical. Conversely, economic efficiency of those frames is decreased if unnecessary special detailing is applied as the beam and column size becomes quite large and steel congestion is caused by joint transverse reinforcement in beam-column connections. Moderate seismic design is used in Korea for beam-column connections of buildings with structural walls, which are to be destroyed when the unexpected large earthquake occurs. Nonetheless, performance of such beamcolumn connections may be substantially improved by the addition of steel fibers. This study was conducted to investigate the effect of steel fibers in reinforced concrete exterior beam-column connections and possibility for the replacement of some joint transverse reinforcement. Ten half-scale beam-column connections with non-seismic details were tested under cyclic loads with two cycles at each drift up to 19 cycles. Main test parameters used were the volume ratio of steel fibers (0%, 1%, 1.5%) and joint transverse reinforcement amount. The test results show that maximum capacity, energy dissipation capacity, shear strength and bond condition are improved with the application of steel fibers to substitute transverse reinforcement of beam-column connections. Furthermore, several shear strength equations for exterior connections were examined, including the proposed equation for steel fiber-reinforced concrete exterior connections with non-seismic details.

A Study on Rumen Fermentation Characteristics and Greenhouse Gas Emission of Forages in South Korea (국내 조사료별 반추위 내 발효특성과 온실가스 발생량에 관한 연구)

  • Young Ho Joo;Ji Yoon Kim;Myeong Ji Seo;Chang Hyun Baeg;Seung Min Jeong;Sam Churl Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.268-273
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    • 2023
  • The present study estimated rumen fermentation characteristics and greenhouse gas emissions of different forages. Alfalfa, timothy, tall fescue, Italian ryegrass, and rice straw as the main forage sources for Hanwoo were used in the present study. Crude protein was highest in alfalfa but lowest in rice straw (p<0.05). Ether extract was higher in alfalfa and Italian ryegrass than in the other forages (p<0.05). Crude ash was highest in rice straw but lowest in tall fescue (p<0.05). Neutral detergent fiber was highest in tall fescue but lowest in alfalfa (p<0.05). Acid detergent fiber was highest in Italian ryegrass and rice straw but lowest in alfalfa (p<0.05). In vitro digestibilities of dry matter (DMD) and neutral detergent fiber (NDFD) were highest in timothy but lowest in rice straw (p<0.05). Rumen pH was highest (p<0.05) in alfalfa, while ammonia-N was higher (p<0.05) in alfalfa and Italian ryegrass than in the other forages. Total volatile fatty acid was highest (p<0.05) in timothy, while acetate and propionate were highest (p<0.05) in alfalfa and rice straw, respectively. Acetate to propionate ratio was higher (p<0.05) in alfalfa, timothy, and Italian ryegrass than in rice straw. Rice straw had lowest total gas (mL) (p<0.05) but highest its per DMD and NDFD. Rice straw had higher (p<0.05) CO2 (per DMD and NDFD) compared to alfalfa (per DMD and NDFD), timothy (per DMD and NDFD), tall fescue (per NDFD), and Italian ryegrass (per DMD). Again, rice straw had higher (p<0.05) CH4 (per DMD and NDFD) compared to timothy (per DMD and NDFD) and tall fescue (per NDFD). Therefore, this study indicates that timothy has a higher nutrient digestibility and volatile fatty acid in the rumen leading to a reduction of greenhouse gas emission.

MANDIBULAR DISTRACTION OSTEOGENESIS WITH COMPRESSION FORCE - BONE DENSITY, HISTOLOGICAL FINDINGS AND TMJ RESPONSE (압축력을 병용한 하악골 신장술)

  • Hwang, Young-Seob;Heo, June;Kim, Uk-Kyu;Park, Seong-Jin;Hwang, Dae-Seok;Kim, Yong-Deok;Chung, In-Kyo;Kim, Kyu-Cheon
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.6
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    • pp.531-548
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    • 2006
  • The purpose of this study was to investigate the biomechanical, histologic findings of distracted regenerate and TMJ response in modified distraction osteogenesis (DO) technique combined with compression force as biomechanical stimulation method which has been suggested in 2002, and developed thereafter by authors. This study was performed with two experiments. First experiment was designed to explore the optimal ratio of compression force versus distraction force for the new DO technique. Second experiment was planned to evaluate the reaction of TMJ tissue, especially condyle, disc after application of the DO technique with compression force. Total 52 New Zealand adult male-rabbits with 3.0kg body weight were used for the study. For the first study, 30 adult male-rabbits underwent osteotomy at one side of mandibular body and a external distraction device was applied on each rabbit with same manner. In the control group of 10 rabbits, final 8 mm of distraction with 1 mm rate per day was done with conventional DO technique after 5 latency days. For the experimental group of 20 rabbits, a compression force with 1 mm rate per day was added to the distracted mandible on 3-latency day after over-distraction (over-lengthening). As the amount of the rate of compression versus distraction, experimental subgroup I (10 rabbits) was set up as 2 mm compression versus 10 mm distraction (1/5) and experimental subgroup II (10 rabbits) was set up as 3 mm compression versus 11 mm distraction (about 1/3). All 30 rabbits were set up to obtain final 8 mm distraction and sacrificed on postoperative 55 day to analysis on biomechanical, and histologic findings of the bone regenerates. For second study, 22 adult male-rabbits were used to evaluate TMJ response after the DO method application with compression force. In the control group, 10 rabbits was used to be performed with conventional DO method, on the other hand, in a experimental group of 10 rabbits, 10 mm distraction with 2 mm compression (1/5 ratio) was done. The remaining 2 rabbits served as the normal control group. Histomorphologic examinations on both condyle, histological studies on condyle, disc were done at 1, 2, 3, 4, 7 weeks after distraction force application. The results were as follows: 1. On the bone density findings, the experimental group II (force ratio - 1/3) showed higher bone density than the other experimental group (force ratio - 1/5) and control group (control group - $0,2906\;g/cm^2$, experimental group I - $0.2961\;g/cm^2$, experimental group II - $0.3328\;g/cm^2$). 2. In the histologic findings, more rapid bone maturation like as wide lamellar bone site, more trabeculae formation was observed in two experimental groups compared to the conventional DO control group. 3. In morphologic findings of condyle, there were no differences of size and architecture in the condyle in the control and experimental groups. 4. In histologic findings of condyles, there were thicker fiberous and proliferative layers in experimental group than those of control group until 2 weeks after distraction with compression force. But, no differences were seen between two groups on 3, 4, 7 weeks after compression. 5. In histologic findings of disc, more collagen contents in extracellular matrix, more regular fiber bundles, and less elastin fibers were seen in experimental group than control group until 2 weeks after distraction with compression. But, no differences were seen between two groups on 3, 4, 7 weeks after distraction with compression. From this study, we could identify that the new distraction osteogenesis technique with compression stimulation might improve the quality of bone regeneration. The no remarkable differences on TMJ response between control and experimental groups were seen and TMJ tissues were recovered similarly to normal TMJ condition after 3 weeks.

Seasonal Variation of Nutritional Intake and Quality in Adults in Longevity Areas (고령인구 비율이 높은 지역 성인 및 노인의 계절별 영양소 섭취실태)

  • 최정숙;백희영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.668-678
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    • 2004
  • The purpose of this study was to estimate seasonal variation of nutritional intake and quality in adults in longevity areas. Dietary survey was given to 469 subjects over age 20 living Bukjeju-gun, Yecheon-gun, and Sunchang-gun of Korea, using 24-hour recall method every 4 seasons over one-year period. The mean daily intakes (%RDA) of 4 seasons were 1313.3 ㎉ (72.0%) for energy, 47.3 g (82.2%) for protein, 20.2 g for fat, 228.0 g for carbohydrate, 12.8 g for dietary fiber. The differences were hardly significant among the seasons. Mean daily intakes of most of the vitamins and minerals were lower than RDA except vitamin C and folate, especially %RDAs of Ca, vitamin D, vitamin B$_2$, vitamin E were less than 60% of RDA. In general, nutrient intake were high in spring compared to other seasons except vitamin C which was high in fall and winter. Mean daily intakes of cholesterol were 151.7 mg and 124.3 mg in males and females, respectively. The differences was significant between the two sexes but not among the seasons. PUFA : MUFA : SFA ratio of the subject was 1.0 : 1.3 : 1.2. Average CPF ratio of energy intake was 72.7 : 14.4 : 12.9, and energy intake ratio from carbohydrate was low in spring, in contrast energy intake ratios from protein was significantly high in spring. Mean adequacy ratio (MAR), an index of overall nutritional quality was 0.64 for female and 0.71 for male. The indices of nutritional quality (INQ) were over 1 for most of nutrients except 0.73 of Ca, 0.87 of vitamin A, 0.69 of vitamin B$_2$, and 0.65 of vitamin E. Both MAR and INQ were significantly different among sex and seasons, values were higher in males than in females and were higher in spring with the exception of vitamin C. In conclusion, subjects in longevity areas did not consume enough nutrients quantitatively as well as qualitatively, especially Ca, vitamin A, vitamin B$_2$, and vitamin E. Also mean daily intakes of most of vitamins and minerals were insufficient in females, and were significantly different among season. Therefore we must consider a counterplan to augment nutrition intake for them. In addition, it seems to be essential to micro nutrients to the food composition database to estimate dietary intakes more accurately.

Effects of Mulberry-Leaf Powder Tofu Consumption on Serum Lipid Profiles, Ca, Ca/P Ratio and Pb Status in Middle-Aged Women (뽕잎분말 첨가두부 섭취가 비만 중년여성의 혈청 지질, 칼슘, 칼슘/인 비율 및 납 수준변화에 미친 영향)

  • Kim, A.J.;Kim, M.H.;Chung, Kun-Sub
    • Korean Journal of Food Science and Technology
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    • v.38 no.3
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    • pp.432-437
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    • 2006
  • This study was carried out to investigate the effects of mulberry-leaf powder Tofu (MPT) on serum lipids profiles, Ca levels, Ca/P ratio and Pb levels in 30 middle aged obese women living in the Choongnam area. 100 g/day MPT was consumed for 4 weeks. The nutrient contents per 100 g MPT were 86.71 kcal (energy), 8.98 g protein, 0.53 mg fiber, 211.33 mg Ca and 1.59 g fat. Anthropometric measurements, 24 h recall dietary intakes, serum levels of protein, albumin, glucose, Ca and Pb, lipid profiles (cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol), and the Ca/P ratio were analyzed before and after consumption of MPT. After consumption of MPT, there were no significant differences in anthropometric measurements, the levels of serum protein, albumin, glucose, total cholesterol, HDL-cholesterol, lipase activity, HTR (HDL-cholesterol/total cholesterol), CRF (cardiac risk factor), Ca, Ca/P ratio and Pb. There were decreases in the levels of serum triglyceride, LDL-cholesterol, AI (atherogenic index) and LHR (LDL-cholesterol/HDL-cholesterol). Significantly increased dietary intakes of plant protein, total Ca, and plant Ca were observed.

Bionomics of Tetranychus urticae Koch on Eggplants under Various Potassium Regimes in Controlled Environment (시설 재배 가지에서 칼리 시비 수준에 따른 점박이응애의 생물적 특성)

  • Kim, Ju;Lee, Sang-Koo;Kim, Jeong-Man;Kim, Tae-Heung;Lim, Ju-Rac;Chon, Hyoung-Gwon;Shin, Yong-Kyu
    • Korean journal of applied entomology
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    • v.47 no.3
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    • pp.217-226
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    • 2008
  • Development of T. urticae was studied on the leaves of eggplant grown in hydroponics with potash contents of 0 mM, 2 mM, 6 mM, and 12 mM. As the levels of potash increased, that of nitrogen decreased and that of P, K, Mg increased in the plant. While contents of crude protein and fiber decreased, those of ash and sugar increased. Carbohydrate content was the highest at 2 mM. Water contents increased as those of potash increased with the exception at 0 mM. Biomass was the heaviest as 552.7 g at 6 mM and the lightest at 0 mM. Leaf thickness and the content of chlorophyll increased as the content of potash increased. Laboratory leaf disc tests provided with various potash levels revealed that feeding and oviposition preferences of T. urticae were high at 6 mM and 12 mM, respectively. Ratio of damaged leaf by naturally occurring T. urticae on eggplants of 99 days post-transplant in the greenhouse was the highest at 6 mM. Development of immature stages of T. urticae shortened as the levels of potash increased with a less tendancy in male than in female. No differences were detected in adult longevity and oviposition period but the number of eggs laid was the most as 84.7 at 6 mM and the least as 40.6 at 0 mM. There were no differences in the rate of egg hatch and the ratio of sex. $R_o,\;r_m,\;and\;{\lambda}$ were the highest at 6 mM and the lowest at 0 mM. T and Dt were the lowest at 6 mM and the highest at 0 mM. There was a descending trand of T. urticae developmet when levels of potash either gets high or low in the hydroponics.

Effects of Uncooked Powdered Food on Antioxidative System and Serum Mineral Concentrations in Rats Fed Unbalanced Diet (생식제품 급여가 영양불균형식이를 섭취하는 흰쥐의 항산화체계 및 혈청 무기질 농도에 미치는 영향)

  • 이여진;이해미;박태선
    • Journal of Nutrition and Health
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    • v.36 no.9
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    • pp.898-907
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    • 2003
  • Antioxidative function of uncooked powdered food (Sangsik) was evaluated in rats consuming nutritionally unbalanced diet including 1% cholesterol, high proportion of animal lipids (lard : soybean oil : 8 . 2) , sub-optimal levels of vitamin and mineral mixture along with 0.5% ethanol in drinking water. The uncooked powdered food tested in the present study was a mixture composed of 42 kinds of plant foods (cereals, legumes, seaweeds, vegetables, and fruits) supplemented with vitamins and minerals, and dietary fiber. Control rats were fed the semi-purified diet based on the AIN-93G composition, and nutritionally unbalanced rats were divided into 3 groups, and fed one of the following diets with 0.5% ethanol in drinking water for 5 weeks : unbalanced control diet (UC) ,20% Sangsik powder supplemented diet (S20), and 40% Sangsik powder supplemented diet (S40) . Food efficiency ratio was significantly higher in rats fed S40 compared to the value for rats fed UC (p < 0.05). Hepatic level of thiobarbituric acid reactive substances (TBARS) was significantly lower in rats fed UC compared to that for control rats (p < 0.05) , and was not influenced by dietary supplementation of the Sangsik powder. Hepatic superoxide dismutase (SOD) activity was significantly higher in rats fed UC compared to that for control rats (p < 0.05) , and significantly reduced in rats fed S20 or S40 compared to the value for unbalanced control rats. Feeding unbalanced control diet significantly reduced the ratio of hepatic GSH-Px + catalase/SOD activities compared to the value for control rats, and this decrease in the ratio of antioxidant enzyme activities was reversed by adding the Sangsik powder to the diet at 20% (p <0.05) . Based on the results of antioxidant enzyme activities, feeding uncooked powdered diet appears to provide a favorable environment for body's antioxidative defense mechanism. Serum levels of Fe and Cu were significantly lower in rats fed the Sangsik powder supplemented diets compared to the value for unbalanced control rats (p < 0.05) , and levels of Se, Mn, and Zn were also tended to be decreased by dietary supplementation of the Sangsik powder. These results postulate the possibility that ingredients used in the uncooked powdered food may decrease the bioavailability of trace elements in rats.

A Study on Components of Pollen Load (花紛荷(pollen load)의 성분조성에 관한 연구)

  • Kim, Jung-Hun;Lee, Kyu-Han;Kim, Eul-Sang;Park, Sung-Bae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.5
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    • pp.566-572
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    • 1992
  • Proximate composition and the contents of free sugars, minerals, fatty acids, amino acids and in vitro digestibility of mixed pollen load were analyzed. The protein in pollen load per 100g was 25.2g, lipid 5.4g, non-fibrous carbohydrate 50.0g and fiber 10.4. Free sugars in pollen load, as dry basis g per 100g, was fructose 26.6g, glucose 13.1g, sucrose 0.2g, maltose 1.3g, melezitose 0.02g and F/G ratio was 2.0. The content of mineral in pollen load was in the order K > P > Mg > Ca > Fe > Na > Mn > Zn > Cu. Unsaturated fatty acid of pollen load was about 69.1% and polyunsalturated fatty acid being about 50.3%. The ratios of SFA : MUFA : PUFA and PUFA : SFA were 0.61 : 0.37 : 1.00 and 1.63 respectively. The total amino acid contained in pollen load 22,337.5mg/100g and total essential amino acid was 39.2% of the total amino acid. proline was the most abundant amino acid (2,824.7 mg/100g),followed by Glu, Leu, Asp, Val, Phe, Ile, Ala and Thr. E/T ratio of protein in pollen load was 2.03 ; protein score, 65.2(Lys) : A/E ratio(egg), 61.7(Lys) : amino acid score, 51.7% after 1hr and on non-fibrous carbohydrate was 80.6%. But that of protein was 67.3% after 1hr, 75.5% after 2hr.

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