• Journal of Embryo Transfer
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• v.7 no.2
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• pp.133-136
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• 1992

Glass wool filtration and swim-up method resulted in inreasing to 58.3% and 62.7% of the progressive motility in frozen-thawed boar sperm, compared to 34.2% in the untreated sperm. Glass wool filtration tended to be more successful than swim-up method for the survival sfter incubation of 38.5$^{\circ}C$ for 3h. Sperm recovered by both the swim-up method and the glass wool filtration method were tested in an in vitro fertilization to determine which of the two techniques would yield sperm with high fertilizing capacity. The results indicated that there was a significantly(p

• Journal of Embryo Transfer
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• v.10 no.1
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• pp.15-22
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• 1995

Since the turn of the century, scientists have earnestly sought to develop a single laboratory assay or combination of laboratory assays which accurately predict the fertility of a semen sample. Most of these assays have focused on evaluating physical characteristics of sperm such as motility, viability, acrosomal integrity and morphology. In recent years new approches have been used to assess the functional aspects of a sperm that are needed to reach the oocyte, fertilize it and contribute to successful embryo development. Among these techniques are the ability of sperm to undergo a heparin induced acrosome reaction and in vitro fertilization, and the affinity of sperm to bind heparin binding protein. Intensification of research efforts in the area of control of sperm fertilizing ability should be a high priority, in view of undoubted benifits both to our basic understanding of sperm fertilizing ability and to our ability to modify it for Al industry.

• Plant Resources
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• v.8 no.1
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• pp.13-16
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• 2005

This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by fertilizing of Sta-Green in pots. The results obtained are summarized as follows. Germination rate of Angelica acutiloba seeds collected in 2003 was $13\%$, while germination rate of seeds Collected in 2004 was above $91\%$. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than seed gathering in 2003. Especially, plant growth and yield of Angelica acutiloba grown in pot(The pots was filled with soil mixtures of Sta-Green and Peat Moss mixed with 45:55 ratio.) was the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by fertilizing of Sta-Green in pots and optimizing seed collecting time in greenhouse.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.02a
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• pp.548-553
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• 2000

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.2
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• pp.178-188
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• 2013

The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 ${\mu}g/ml$ of one of four GAGs (Chondroitin sulfate, CS; Dermatan sulfate, DS; Hyaluronic acid, HA; Heparin, HP) for 5 h, the total motility (TM), straight-line velocity (VSL), and curvilinear velocity (VCL) were higher in the HP- or HA-treated sperm, relative to control and CS- or DS-treated sperm. HP and HA treatments increased the levels of capacitated and acrosome-reacted sperm over time, compared to other treatment groups (p<0.05). In addition, sperm exposed to HP or HA for 1 h before IVF exhibited significantly improved fertilizing ability, as assessed by 2 pronucleus (PN) formation and cleavage rates at d 2. Exposure to these GAGs also enhanced in vitro embryo development rates and embryo quality, and increased the ICM and total blastocyst cell numbers at d 8 after IVF (p<0.05). A real-time PCR analysis showed that the expression levels of pluripotency (Oct 4), cell growth (Glut 5), and anti-apoptosis (Bax inhibitor) genes were significantly higher in embryos derived from HA- or HP-treated sperm than in control or other treatment groups, while pro-apoptotic gene expression (caspase-3) was significantly lower in all GAG treatment groups (p<0.05). These results demonstrated that exposure of bovine sperm to HP or HA positively correlates with in vitro fertilizing ability, in vitro embryo developmental potential, and embryonic gene expression.

• Clinical and Experimental Reproductive Medicine
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• v.23 no.1
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• pp.81-86
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• 1996

The assessment of acrosomal status is important in evaluating the ability of sperm to fertilize the egg. The acrosomal status of sperm from 47 normal volunteers with proven fertility and 167 subfertile men with not to achieve pregnancy for at least 1 year were evaluated with Acrobeads test(FUSO Pharmaceutical Industries, Ltd, Japan) using immunobeads coated with MH61 monoclonal antibody, which is specific for acrosome-reacted sperm. The mean${\pm}$SD of acrobeads score in 47 volunteer group was $2.8{\pm}0.7$, of which 46(97.9%)cases were ${\geq}$ 2. The mean${\pm}$SD of acrobeads score in 167 subfertile group was $1.7{\pm}0.8$, of which 73(79.3%)cases were ${\leq}$ 1. The aerobe ads score in subfertile group were significantly lower(r=0.294, p<0.05) than those in volunteer group. In subfertile group, acrobeads score were well correlated with the sperm density and motility(r=0.275, r=0.281, p<0.01), but not with semen volume(r=0.16) and serum hormone level(FSH r=0.084, LH r=0.036, testosterone r=0.058, prolactin r=0.006 and estradiol r=0.060)(p>0.05). Of 63 subfertile cases with normozoospermia, 22(34.9%)cases showed 0 or 1 of acrobeads score, which means to accompany with a functional defect in spite of normal morphology. As a results, Acrobeads test is not only a technically simple sensitive procedure with good reproducibility in evaluating the sperm fertilizing capacity but also an useful in the evaluation of effectiveness in the treatment of infertility and the separation of acrosome-reacted sperm in the assisted reproductive technique.

• Clinical and Experimental Reproductive Medicine
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• v.18 no.1
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• pp.73-80
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• 1991

We compared fertilizing potential measurements by the zona-free hamster egg penetration assay with the in vitro fertilization and embryo transfer program was evaevulated for their ability to fertilize zona free hamster egg. Spermatozoa from 12 presumeably fertile donors and from the male partners of 56 infertile couples were evaluated for their ability to fertilizing potentials. Penertration rates of fertile donors were $36.2{\pm}27.7%$ ; Fertilization rates of infertile couples between with normal semen parameters and with abnormal semen parameters were $28.7{\pm}19.1$, $5.7{\pm}8.9%$, respectively. Sperm motility of couples with penetration rates between on 15-30% and on 30> were $54.1{\pm}4.6$, $55.5{\pm}8.3%$ respectively. Hamster penetration rates of couples participating in an in vitro fertilization and embryo transfer program was $38.9{\pm}29.9%$. But in one case, a positive fertility assessment was obtained in the absence of fertilization of the wife's eggs attributable to egg immaturity. This method may have potential value as a diagnostic tool in evaluation human sperm fertilization capacity which avoids the ethical and logistical problems associated with fertilizing of human eggs in vitro.

• Journal of Biosystems Engineering
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• v.31 no.3 s.116
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• pp.203-208
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• 2006

This study was conducted to improve the control performance of a current variable-rate controller for granular fertilizers. Simulation model was developed. Optimized proportional, integral and derivative gains were determined by simulation model using 2nd order PID gain learning algorithm, and these control gains were evaluated through the field tests. Important results of this study are as follows; 1. Principles of pre-existing variable-rate application of granular fertilizers were investigated. 2. Simulation model of a PID controller that could simulate the control system was developed by using Matlab/Simulink program. The program was to determine PID control coefficients through the simulation model and 2nd order PID gain learning algorithm. 3. PID control coefficients obtained from the simulation were applied to the developed model. When the step input was given, Maximum overshoot were 1.96%, rise time were 0.05 sec, settling time were 0.06 sec and steady state error were 0.21 % respectively. 4. The simulation model was verified through field tests. The errors of maximum overshoot were 10%, rise time were 0.11 sec, settling time were 0.40 sec and steady state error were 8% because of loads and noises. Rise time was decreased to one third of that of the pre-existing system. 5. If the speed of a fertilizing machine is $0.3{\sim}0.6\;m/s$ and the maximum rotation speed of a discharging roller is 64 rpm, rise time would be 0.26 sec and fertilizing machine would cover the distance of $0.07{\sim}0.15\;m$ with settling time of 0.4 sec, fertilizing machine would cover the distance of $0.12{\sim}0.24\;m$.

• Korean Journal of Medicinal Crop Science
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• v.22 no.2
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• pp.127-133
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• 2014

This experiment was carried out to investigate the effect of several organic compost on the growth and root yield of Angelica gigas Nakai with organic cultivation. After fertilizing the soil with organic fertilizer, the dry weight of liming fertilizer showed a slow change, while microorganism fertilizer decreased about 29% until 20 day after fertilizing. At 110 days after fertilizing, microorganism fertilizer decreased more than liming fertilizer. Liming fertilizer decomposed slowly, while microorganism fertilizer decomposed early on rapidly but gently after 20 days in decomposed rate of organic fertilizer. Dried root yields per 10a of A. gigas were not significance between 277.6kg in conventional fertilizer and 277.7kg, 280.5kg in N 1.5, N 2.0 times of microorganism fertilizer. Decursin and decursinol angelate contents in A. gigas were 9.08 ~ 9.07% from N 1.0 and N 1.5 times in liming fertilizer, and 7.94 ~ 8.12% from N 1.5 times and N 2.0 times in microorganism fertilizer, compared to 7.31% of conventional treatment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.69-75
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• 1994

This experiment was canied out to study the effect of cutting frequencies (3, 4 and 5 cuts per year) and mineral nitrogen fertilization on dry matter yields, in order to estimate optimum level of fertilizing mineral nitrogen in orchardgrass(Dacry1is glomerafa L.). The results are as follows: 1. Dry matter yields were the highest in the 1st cut at 3 cutting frequency and in the 2nd cut at 4 and 5 cutting frequencies, and they shared 49.7, 37.0 and 37.0% of annual dry matter yield respectively. 2. When only phosphorus and potassium were applied, annual dry matter yields were between 9.4 and 11.5 tons per ha and the highest yield was observed at 3 cutting frequency. 3. Dry matter yield in relation to fertilization of mineral nitrogen was highly increased when more nitrogen was applied as 360 kg per ha compared to 240 kg per ha at 3 cutting frequency. At 4 and 5 cutting frequencies, increases in dry matter yield, to large extent, were showed at 120 and 150 kg of nitrogen per ha(30 kg Nlcutlha) compared to no application of nitrogen. The efficiencies of mineral nitrogen fertilization (kg DM/kg N) were 28.0, 22.7 and 20.6 kg dry matter yields per nitrogen(kg) respectively). 4. At 4 and 5 cutting frequencies, dry matter yields were reduced when 360 kg and 300 kg of mineral nitrogen per ha(90 kg and 60 kg Nlcutlha) were applied respectively. 5. Particularly at 4 and 5 cutting frequencies of this study, maximum marginal yields reached at 129.9 kg and 148.0 kg of fertilizing mineral nitrogen per ha, and economical borden of mineral nitrogen fertilization were between 222.4 and 250.0 kg and between 244.8 and 276.8 kg respectively. At the same cutting frequencies, the highest dry matter yields were obtained at 365.4 and 433.8 kg of fertilizing mineral nitrogen respectively.