• Title/Summary/Keyword: Fertilization and hatching

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Studies on the Seedling Production of Long Shanny, Stichaeus grigoriewi Herzenstein 1. Natural Spawning in Indoor Tanks and Embryonic Development (장갱이, Stichaeus grigorjewi Herzenstein의 종묘생산에 관한 연구 1. 실내에서의 자연산란과 난발생)

  • Lee Jung-Uie;Jo Jae-Yoon
    • Journal of Aquaculture
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    • v.8 no.3
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    • pp.195-207
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    • 1995
  • In order to study the embryonic development and hatching of wild long shanny, Stichaeus grigorjewi, were caught with the gill nets in the East Sea of Korea, and stocked at indoor tanks to induce natural spawning in February 25, 1994 and February 16 to 24, 1995. They were already matured when stocked, and average body length (50.66 cm) and body weight (1,192.74 g) of 57 females and average body length (48.62 cm) and body weight (612.58g) of 43 males were recorded. Before stocking, they were inserted with identification tags(ID tags) in the dorsal muscle, and spawning was traced by the portable reader (Destron/lDl Ltd.) Forty females among 57 spawned successfully in the average of 4 days after stocking. Females spawned almost all eggs contained in the ovaries at one time in the form of an egg mass and averaging 227,200 eggs Per egg mass. The egg mass was oval in shape, translucent milky in color, 20.32cm long axis and 14.57cm short axis in size, and 803.7g in weight. Male parents guarded their egg masses and circulated water with the tail part of the body. Fertilized egg was spherical in shape, and their average diameter was 1.54 mm. Each egg had a containing single oil globule, and it's average diameter was 0.37 mm. The average water temperature was $13.2^{\circ}C$ and incubation times after fertilization were 5 hours 25 minutes up to 2-cell stage, 13 hours up to morula stage, and 66 hours 35 minutes up to embryo formation stage. Hatching rate was approximately 10 percent in 368 hours 50 minutes after fertilization, and approxionateoly 90 percent of eggs were hatched in 425 hours 30 minutes after fertilization.

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Correlationship between Artificial Maturation Season and Reproduction Coefficient in the Cultured Eel Anguilla japonica (양식산 뱀장어 Anguilla japonica에 있어서 인공성성숙시기와 번식률과의 상관관계)

  • Bae, Jun-Young;Kim, Dae-Jung;Lee, Jung-Uie;Son, Sang-Gyu;Lee, Jong-Kwan
    • Journal of Aquaculture
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    • v.20 no.4
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    • pp.219-225
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    • 2007
  • This study investigated the correlationship between artificial maturation season and reproduction coefficient of cultured eel Anguilla japonica from May (spring) to next January (winter). The brood stock, female eels ($400{\sim}600\;g$) were artificially matured by weekly intramuscular injections of salmon pituitary extracts (SPE, 20 mg/fish) to induce a completion of vitellogenesis. After completion of vitellogenesis, final oocyte maturation and ovulation was induced by injection of $17{\alpha}$, $20{\beta}-dihydroxyprogesterone$ (DHP) at about $2\;{\mu}g/g$ body weight. Most fish ovulated $15{\sim}18\;h$ following the DHP injection. The ovulated fish were induced to natural spawning or artificial fertilization by the dry method. Males ($200{\sim}350\;g$) were received weekly intramuscular injections of human chorionic gonadotropin (HCG) at a dosage of 1 IU/g body weight to induce testicular maturation and spermiation. Seasonal reproduction coefficient which includes the rate of ovulation, buoyancy, fertilization and hatching of eggs in the artificially matured eel during spring to summer ($May{\sim}July$) were significantly higher than the other season, while there were no significant difference among spring and summer (P<0.05). Furthermore, the number of eggs spawned and larvae hatched in the artificially maturated eel during spring to summer ($May{\sim}July$) were significantly higher than the other season, while there were no significant difference in spring and summer (P<0.05). These results indicate that artificial maturation by hormone treatment of A. japonica was successful only during spring to summer, which is the maturation period in the wild stock in nature. Consequently, it is possible to determine the period of artificially induced sexual maturity by the reproduction coefficient which includes the rate of ovulation, buoyancy, fertilization and hatching of eggs in the cultured eel A. japonica.

STUDIES ON THE PROPAGATION OF TOP SHELL-I Spawning and early development of the top shell, Turbo cornutus SOLANDER (소라 Turbo cornutus SOLANDER의 증식에 관한 연구-I 소라의 산란과 초기발생)

  • RHO Sum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.1
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    • pp.43-55
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    • 1976
  • Fertilization and early development of turbo cornutus was studied based on the samples which were collected in Yeosu area. Particular emphasis was paid on induction of artificial spawing, fertilization rate, preembryonic development, the growth of the early larva and larval survival to various salinity. Among the various methods for induction of artificial spawning which have been tested for the present study, drying by exposure to air is the. most efficient, and percentage fertilization rate was $83.8-96.4\%$. The diameter of fertilized eggs was $0.182{\pm}0.0028mm$; and the diameter of egg membrane was $0.245{\pm}0.093mm$. Under the temperature range of $20.6-25.4^{\circ}C$ the larvae hatched out after 11:05-11:15 hours of fertilization. After 3.0-3.5 days of fertilization the planktonic larvae begand to settle, and the settlement terminated within 5 days. During the period of 150 days of early culturing the diameter growth of shell(M) and the diameter of shell aperture(A) was formulated as follows: $$1972\;M=0.33e^{0.02070D}$$ $$A=0.19e^{0.02282D}$$ $$1973\;M=0.32e^{0.02282D}$$ $$A=0.16e^{0.02596D}$$ During the same period of early culturing the relative growth of shell diameter and the diameter of shell aperture was formulated as follows : 1972 A=0.6478 S-0.1575 1973 A=0.5897 S-0.0515 After 11 days of larval hatching $0.02-0.18\%$ of planktonic larvae settled. After 150 days of settlement the survival rate of the early shells was $7.4-21.6\%$. Under the temperature range of $21.0-22.7^{\circ}C$ the optimum salinity range for the development of egg and the planktonic larvae was $30-35\%_{\circ}$.

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Studies on the Morphological Characteristics and Life History of the Chili Fish, Cultriculus eigenmanni (Jordan et Metz) (1) On the Egg Development and the Fry in the Larval Stage (치리(Cultriculus eigenmanni)의 형태 및 생활사에 관한 연구 I. 난발생과 부화자어에 대하여)

  • KIM Eul-Bae;KIM Young-Gill
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.151-156
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    • 1984
  • The Chili fish, one of the most common species of the freshwater fishes, is distributed in the river basins of the western coast of Korea. However, any studies on this fish, especially its development or life history, have not been made so far. The authors, therefore, carried out an experiment to reveal some characteristics of egg develoment and fry in this fish. For accomplshing this study, we caught some adult Chili fish by means of a casting net at raceway in the vicinity of Kunsan in June and July, 1983; then, we injected 0. 1 cc Gonadotrophic hormone 1,000 unit solution (Puberogen) in order to induce the spawning on the abdominal cavity of male and female respectively, Approximately 12 hours after injection, the artificially fertilized eggs by using of the dry method and the naturally fertilized eggs were observed simultaneously in the aquarium ($30{\times}50{\times}70cm$). The fertilized eggs, in spherical shape, were sperative, demersel, adhesive nature and $1.38{\sim}1.59\;mm$ in diameter. The light yellow yolk was opaque and $1.17{\sim}l.27\;mm$ in diameter. The hatching of eggs took place at 29.5 hours after fertilization under the condition of $23.5{\sim}25.0^{\circ}C$ water temperature. Basically there were not found so many different aspects in comparison with the common species of Cyprinidae in the development process of eggs : however, there were a few noticable characteristics, that is, pigment cells, eye balls and blood circulation are invisible, or the velocity of egg development is rapid, seemed similar to the Loach's egg. Newly hatched larvae with the yolk sac (2.39 mm in size) were $3.87{\sim}3.99\;mm$ in total length and their myotome number was expressed as 28+15=43. About 20 hours after hatching, a caudal and a pectoral fin as well as an anus began to orgin. In addition, a few malanophore showed on the upper regin of the yolk sac of the larvae whose size was 4.73 mm in total length. The larvae developed about 5.02 mm in total length, and the median fin fold in the abdomen extended forward to two thirds or beyond of the yolk sac at 50 hours after hatching. Simultaneously, the eye balls and mouth also appeared. After 3 days, the yolk sac nearly disappeared, and the larvae (5.4 mm in total length) with perfect eye balls began to swim for themselves for the first time. Also, the specialization between the gill arches and the ailmentary canal, the development of each fins except dorsal fin as well as the blood circulation obviously distinguished in this stage. The larvae whose total length was 5.68 mm was very brisk: also, the development of the dorsal fin rudiment, nearly perfect formation of each organs and the function of the upper and lower jaws were noticeably observed 5 days after hatching.

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Egg Development and Larvae of the Rose Bitterling Rhodeus ocellatus (KNER) (흰줄납줄개의 난발생과 부화자어)

  • KIM Yong Uk;PARK Yang Seong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.6
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    • pp.586-593
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    • 1985
  • The rose bitterling, Rhodeus ocellatus (KNER) is commonly distributed in the fresh waters of Korea and Japan. On January 15, February 15, and March 18 in 1984, mature adults of rose bitterling were caught in the watercourse of Maeri, Kimhae, South Korea. The authors fertilized the eggs employing dry method in the laboratory on May 7, 16 and 25 in 1984. Hatched larvae were reared in small aquariums at $17{\sim}25.5^{\circ}C$ (average around $21.2^{\circ}C$). Mollusks, Anodonta woodiana in the gill chamber of which, rose bitterling lay eggs were caught in order to study natural spawning of the rose bitterling in the same watercourse. The eggs of this species are not adhesive and demersal. The size of the eggs varies from 2.54 to 2.75mm in long diameter and 1.45 to 1.65mm in short diameter. The eggs are cylindrical in form when they are extruded from ovipositor, immediately after entering water, but they acquire their distinctive form of a greatly elongated pear. Hatching took place in ca. 39 hours after fertilization. The newly hatched larvae were $2.65{\sim}2.70mm$ in total length possessing yolk sac and 13-14 myotomes. Thirteen days after hatching, the prelarvae attained 6.5 mm in total length, and the first melanophores appeared on the head, and the anterior part and sides of the yolk sac. One month after hatching, the postlarvae attained 8.5mm. in total length and emerged from the gills of the mollusks. Then the yolk sac was completely resorbed. Two months after hatching, the rose bitterling attained 14.4mm in total length, and entered the fingerling period of life. All the rays already present were the D. III, 11-12, A. III, 11-12, P. 10, V. 7. and a distinguishing, feature is the presence of a black pigment spot in the lobe of the dorsal fin.

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Naturally Collection and Development until Yolk Absorption of Domestic Walleye Pollock Theragra chalcogramma Fertilized Eggs and Larvae (국내 명태 Theragra chalcogramma 자연채란과 난황흡수까지의 난 발생)

  • Seo, Joo-young;Kwon, O-Nam
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.1
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    • pp.49-54
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    • 2017
  • We collected and reared Theragra chalcogramma walleye pollock brood-stock for use in natural spawning tests and undertook to obtain domestic pollock via fertilized egg capture, development of fertilized eggs, and absorption of yolk sac after hatching. Whole pollock were caught with trammel and set nets and immediately placed in a deep-sea water tank. Adults were the most common pollock age group (43.0%; n = 86) among the 254 pollock captured in March 2014 with 57.9% (n = 147) being captured off Southern Gosung, Korea. The main spawning period of pollock is February (spawning phase of 91% of pollock). From the deep-sea tank, we collected 1640 mL of naturally fertilized eggs (~820,000 eggs) from 12 spawning events occurring between February 4 and 22 2015. The floating/ live eggs were maintained in deep-sea water tanks at $5.5{\pm}0.2^{\circ}C$. Egg size was $1.5{\pm}0.03mm$. Six hours after fertilization the eggs were at the 2 cell stage, and the eggs hatched approximately 340 hours after collection. At hatching, larval length and yolk sac area were $5.2{\pm}0.25mm$ and $9.5{\pm}1.00mm^2$ (100%), respectively. Four days after hatching, the yolk sac area was $2.2{\pm}0.53mm^2$ ($23.1{\pm}5.55%$). This is the first report of collection of naturally fertilized eggs from pollock and their subsequent hatching while held in an indoor deep-sea water tank. The results suggest that such collection could assist in the recovery of pollock resources and the possibility of domestic rearing of cultivated larvae.

Spawning Behavior and Early Life History of Aucha Perch, Coreperca kawamebari from Korea (한국산 꺽저기 (Coreoperca kawamebari)의 산란습성(産卵習性) 및 초기생활사(初期生活史))

  • Han, Kyeong-Ho;Park, Joon-Taek;Kim, Byeong-Mu;Oh, Sung-Hyun;Lee, Sung-Hun;Jin, Dong-Soo
    • Korean Journal of Ichthyology
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    • v.12 no.2
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    • pp.129-136
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    • 2000
  • The aucha perch, Coreoperca kawamebari was collected in Tam-jin river from February to June 1998. It was reared in the laboratory and observed the spawning behavior and early life history. Spawning season was from mid of April to the end of May in the Tam-jin river. The fertilized eggs were demersal of adhesive, transparent and spherical in shape. Egg diameter was 2.21~2.65 mm with several oil globule of 0.058~0.343 mm. Hatching occurred about 194 hours 23 minutes after fertilization at water temperature of $18{\sim}22^{\circ}C$. Newly-hatched larvae were 5.09~5.68 mm in total length(TL, mean: 5.38mm) with 10~11+18=28~29 myotomes and opened mouth and anus. Melanophores were distributed on the eye lens, on the head, around the yolk, on the dorsal part and the abdominal region of the trunk. After hatching 5 days larvae attained 6.12~6.68 mm in TL (mean: 6.47 mm), and the yolk sac was completely absorbed and transformed to postlarva stage. The larvae reached to the juvenile stage with all the fins were formed with complete set of fin rays (D. XII-12~13; A. III-8~10; P. 11~13; V. I-4~5) at the 22 days after hatching and of the larvae was 11.54 mm in total length. In 32 days after hatching, the juvenile was 13.05 mm in TL. This period was similar to adult in body form and the spot.

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Spawning Behavior and Development of Eggs and Larvae of the Korea Freshwater Goby, Rhinogobius brunneus (Gobiidae: Perciformes) (한국산 밀어 (Rhinogobius brunneus)의 산란습성, 난발생 및 자어의 형태발달)

  • HAN Kyeong-Ho;KIM Yong Uk;CHOE Kyu-Jung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.1
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    • pp.114-120
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    • 1998
  • The spawning behavior, development of eggs and larvae of the Korea freshwater goby, Rhinogobius brunneus (Temminck et Schlegel) were studied. The eggs were spawned as a one-layer mass, hanging from the underside of a small pebble, and guarded by one male. The eggs were elliptic, about 1.48 mm in length and 0.65 mm in breadth, with a round top and a somewhat flat base with glutinous fibers. Hatching in the indoor tank with $17.0^{\circ}C$ in mean water temperature started from the 146 hours after fertilization. In the late developing stages, the embryo moved and freely changed its head to face the free tip of the chorion (normal egg) or to the basal end with a boundle of adhesive filaments (agrippa egg). Newly-hatched larvae were $3.10\~3.30$ mm in total length (mean: 3.22 mm), and mouth and anus were not yet open. Melanophores were present on the air- bladder, around the anus, and on the ventral part of the caudal region. The larva $3\~4$ days old transformed to postlarval stage, and they were $3.30\~3.85$ mm in total length (mean: 3.60 mm). As yolk sac and oil globules werw nearly absorbed, mouth and anus were open, and they fed rotifers actively. In $20\~22$ days after hatching the larvae grew to 5.85 mm in 71, and the caudal notochord flex at $45^{\circ}$.

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Spawning Behavior and Early Life History of Korean Aucha Perch, Coreoperca herzi Herzenstein (한국산 꺽지 (Coreoperca herzi)의 산란습성 및 초기생활사)

  • 한경호;박준택;정규화;이원교;이재용;방인철
    • Journal of Aquaculture
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    • v.11 no.1
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    • pp.49-58
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    • 1998
  • The spawning behavior and early life history of the Korean aucha perch, Coreoperca herzi were studied. The eggs were spawned in a one-layer mass, hanging from the underside of a largy stone and guarded by one male. According to the considerable size difference os eggs in the individual ovaries, it was concluded that they spawns several times within a spawning season of May~June. The ripe eggs were demersal and adhesive, and their diameter were 2.60~3.04mm with several oil globule of 0.10~0.64mm. Hatching in the tank with $19.0{\pm}1.0^{\circ}C.$ in men water temperature started from the 380 hours after fertilization. Newly-hatched larvae were measured 6.45~7.34mm in total length (TL, mean : 6.85mm), and mouth and anus were open. Melanophores were present on the head, around the yolk, and on the dorsal part of the abdominal region. 3 days old larvae were measured 7.00~7.40mm in TL (mean : 7.25mm), and they fed Daphnia sp. and Brachionus plicatilis actively. 6 days old larvae transformed to postlarval stage and they were 7.94~10.10mm in TL (mean : 9.00mm). As yolk sac were nearly absorbed, the caudal notochord flex at 45. Aggregate numbers of all fin rays were completed at over 16.90~19.80mm in TL (30 days after hatching), at which time the larvae reached the juvenile stage. In fifty days old larvae(24.60~27.10mm in TL) were similar in body form, lateral line and color to adult.

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Morphological Development of Embryo, Larvae and Juvenile in Yellowtail kingfish, Seriola lalandi

  • Yang, Sang Geun;Hur, Sang Woo;Ji, Seung Cheol;Lim, Sang Gu;Kim, Bong Seok;Jeong, Minhwan;Lee, Chi Hoon;Lee, Young-Don
    • Development and Reproduction
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    • v.20 no.2
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    • pp.109-118
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    • 2016
  • This study monitored the morphological development of embryo, larvae and juvenile yellowtail kingfish, Seriola lalandi, for their aquaculture. The fertilized eggs obtained by natural spawning were spherical shape and buoyant. Fertilized eggs were transparent and had one oil globule in the yolk, with an egg diameter of $1.35{\pm}0.04mm$ and an oil globule diameter of $0.32{\pm}0.02mm$. The fertilized eggs hatched 67-75 h after fertilization in water at $20{\pm}0.5^{\circ}C$. The total length (TL) of the hatched larvae was $3.62{\pm}0.16mm$. During hatching, the larvae, with their mouth and anus not yet opened. The yolk was completely absorbed 3 days after hatching (DAH), while the TL of post-larvae was $4.72{\pm}0.07mm$. At 40 DAH, the juveniles had grown to $30.44{\pm}4.07mm$ in TL, body depth increased, the body color changed to a black, yellow, and light gray-blue color, and 3-4 vertical stripes appeared. At 45 DAH, the juveniles were $38.67{\pm}5.65mm$ in TL and $10.10{\pm}0.94mm$ in body depth. The fish were green with a light orange color, with 7 faint green-brown stripes on the sides of their body. At 87 DAH, the juveniles had grown to 236.11 mm in TL, 217.68 mm in fork length, and 136.5 g in weight. The fish resembled their adult form, with a light yellow-green body color, loss of the pattern on the sides of their body, and a yellow coloration at the tip of the caudal fin.