• KSBB Journal
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• 제21권2호
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• pp.103-109
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• 2006

H. erinaceum의 기능성 소재화 연구의 일환으로, 지금까지 체계적으로 연구된 바 없는 H. erinaceum의 액체배양을 시도하고. 이 버섯의 서로 다른 생물반응기하의 액체배양 특성을 규명하고 대량생산을 검토하였다. 5 L 용량의 jar fermenter와 air-lift fermenter에서의 액체배양의 비교실험을 통해 air-lift fermenter의 사용이 종균 배양에 적합한 것으로 나타났으며, 형태 분석을 통해 적정 크기($2{\sim}4mm$)의 pellet 형태의 유지가 대량 생산의 중요인자인 것을 규명하였다. Air-lift fermenter 하의 배양을 종균으로 하고, 적정 크기의 pellet을 유지하는 500 L까지의 jar fermenter를 사용한 회분 배양의 scale-up 실험결과, 30 및 500 L에서의 교반 및 통기속도는 각각 200 rpm, 1 vvm 및 150 rpm, 1 vvm이었다. 이 조건하에서 배양의 전 기간 중 적정 pellet을 유지하였으며, 최대 균사체량은 약 $13{\sim}14g/L$이었다.

• 한국물환경학회지
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• 제20권2호
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• pp.183-189
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• 2004

The performance of a membrane-coupled anaerobic fermenter system for the recovery of volatile fatty acids (VFAs) from liquid organic sludge was experimentally investigated. Permeation flux was stably kept around $0.2(m^3/m^2/day)$ during operational period. The membrane-coupled fermenter showed 2.2 times higher VFAs concentration and higher VFAs forming rate than those of fermenter without membrane. The fermenter with membrane proved to be an effective system for the recovery of soluble organic materials from liquid sludge.

• 현장농수산연구지
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• 제10권1호
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• pp.169-181
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• 2008

The purpose of this study was to produce Pleurotus ostreatus using fermenter with bag cultivation. These results are as follows. 1. While mushroom composts were being fermented in a fermenter, the physical property of the fermented composts was getting better when there isn't any screw or revolving flies in the fermenter and the strength of pressing the composts was getting less. 2. The composts were fermented well as slaked lime of 1% density added to the composts. 3. According to the result of examining our fermenting ways, composts were in the best condition after being fermented for 48 hours since the temperature in a fermenter has come to 60℃, which could be reached by heating the fermenter by 40℃ after putting compost materials and water into it. 4. The good condition of fermenting could be maintained by controlling the speed of revolving flies, therefore the speed be down when the temperature is above 60℃ and up bellow 60℃. 5. Since the composts had been added with 1.5~2% of cottonseed meal or rice bran, the fermented composts were in good condition and also the quantity and quality of the mushroom produced on the fermented composts were satisfied. 6. There were needed 7 hours of labour for 3days from the first day of putting composts into a fermenter for fermenting 3.5M/T(10,000~12,000bags of 750~800g per bag) of composts to the third day of finishing the fermenting work, and also the cost was 112,066￦(130$) including 52,066￦(60$) of electric charge and fuel expense.

• 한국식품저장유통학회지
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• 제7권1호
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• pp.94-98
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• 2000

To produce liquor and vinegar using potatoes needs to liquefy and sacchrify potatoes . So selecting the efficient fermenter for proceeding these process successfully is very important . This study was investigated several fermenter and crush types of potatoes for alcohol fermentation. Final sugar contents was high in pottoes saccharificatiion by nuruk or crude enzyme. But pure enzyme and blucoamylase ended liquefaction and saccharificatiion within short ime. So complex type fermenter mixed several fermenters was superior to single type fermenter. Complexfermenter III using crude enzyme and glucoamyulase saccharificed excellently potatoes with 150% of water contents by treatment of 3 hours. Through alcohol fermentation using pressure steamed potatoes (PSP), it could be obtained 6.4% , 150%, of alcohol content and yield. However to perform a series process efficiently , crush steamed pottoes (CSP) was suitable. When it was fermented after saccharification using crush steamed potatoes and complex fermenter III, it could be obtained 6.6% of alcohol and 6.7% of acidity.

• 멤브레인
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• 제14권1호
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• pp.37-43
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• 2004

휘발성 지방산의 생성 및 회수를 위한 분리막의 유효성을 실험적으로 검토한 결과, 분리막의 적용에 의해 발효조 내의 부유고형물 농도, 유기산 생성균수 및 유기산 농도가 증가하였다. 혐기성 발효액의 고액분리 및 발효 효율향상을 위한 분리막의 적용은 발효조 내의 관련 미생물 농도를 증가시키고 따라서 분리막을 적용하지 않은 경우에 비해 유기산 생성효율이 훨씬 증가하였다. 분리막이 결합된 산 발효조의 적용은 유기성 슬러지로부터 휘발성 지방산의 회수 효율증대에 효과적인 적용기술이라 판단된다.

• KSBB Journal
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• 제15권3호
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• pp.280-284
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• 2000

The simple imcroprocessor device was provided for the control of dissolved oxygen(DO) level. The DO analog signal output from fementer was used as a measurement variable and the agitation speed of fermenter was used as a manipulatio variable. Agitation speed was manipulated to maintain DO level above 10% of saturation condition during cell growth period with microprocessor. Since the experimental resultsshowed that the DO level was maintained above 10% and agitation speed was inverse proportional to DO level ithis simple control device can be used for the DO control of fermenter.

• KSBB Journal
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• 제13권5호
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• pp.547-553
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• 1998

For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

• Journal of Microbiology and Biotechnology
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• 제22권1호
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• pp.92-99
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• 2012

The optimal physical factors affecting enzyme production in an airlift fermenter have not been studied so far. Therefore, the physical parameters such as aeration rate, pH, and temperature affecting PLA-degrading enzyme production by Actinomadura keratinilytica strain T16-1 in a 3 l airlift fermenter were investigated. The response surface methodology (RSM) was used to optimize PLA-degrading enzyme production by implementing the central composite design. The optimal conditions for higher production of PLA-degrading enzyme were aeration rate of 0.43 vvm, pH of 6.85, and temperature at $46^{\circ}C$. Under these conditions, the model predicted a PLA-degrading activity of 254 U/ml. Verification of the optimization showed that PLA-degrading enzyme production of 257 U/ml was observed after 3 days cultivation under the optimal conditions in a 3 l airlift fermenter. The production under the optimized condition in the airlift fermenter was higher than un-optimized condition by 1.7 folds and 12 folds with un-optimized medium or condition in shake flasks. This is the first report on the optimization of environmental conditions for improvement of PLA-degrading enzyme production in a 3 l airlift fermenter by using a statistical analysis method. Moreover, the crude PLA-degrading enzyme could be adsorbed to the substrate and degraded PLA powder to produce lactic acid as degradation products. Therefore, this incident indicates that PLA-degrading enzyme produced by Actinomadura keratinilytica NBRC 104111 strain T16-1 has a potential to degrade PLA to lactic acid as a monomer and can be used for the recycle of PLA polymer.

• 상하수도학회지
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• 제22권3호
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• pp.299-306
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• 2008

This study has been conducted to derive the bio-energy, hydrogen and methane production, from mixture of food wastewater and swine wastewater, the high strength organic wastewater and to increase effluent quality. To overcome this limitation in one-phase anaerobic process, two-phase anaerobic process combining hydrogen fermenter and methane fermenter was applied. In this system $2,323ml\;H_2/L$ was produced daily from Run II where 500 ml of heattreated sludge in methane fermenter was injected, and methane produced from methane fermenter did not show big difference regardless of the amount of returning sludge at each Run. It was concluded that the two-phase anaerobic process was the appropriat process to produce hydrogen and methane simultaneously and stably. Influent $TCOD_{Cr}$ to two-phase anaerobic process showed the range of 132~145 g/L(average 140 g/L), and effluent $TCOD_{Cr}$ range was 25~40 g/L(average 32 g/L), and organic removal efficiency showed 71~82%(average 76.3%).

• Preventive Nutrition and Food Science
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• 제7권1호
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• pp.28-32
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• 2002

A Micrococcus sp. producing catalase was isolated from soil, and a commercial-scathe cultivation and purification of catalase were conducted. The maximum catalase activity was about 103 BU/mL obtained after 46 hr of cultivation in a 30 L fermenter containing 2% glucose, 2% peptone, 4% yeast extract, and 0.5% NaCl. Soybean sauce, CSL (corn steep liquor), and yeast extract were also studied as media substitutes in the media 30 L fermenter. The optimum medium components for the production catalase were found to be 2% glucose, 4% soybean sauce, and 16% CSL. In a 18 kL fermenter, the stationary phase in the cell growth and maximum catalase activity (112 BU/mL) were reached after 46 hr of cultivation, which was the same result as in the 30 L fermenter. The catalase activity was purified with over 17 folds in four steps with a 33.6% yield. From 104,250 mg of protein after cell lysis, 1,966 mg of the purified enzyme with a specific activity of 192.7 kBU/mg was obtained. The residual activity with the addition of 10% NaCl exhibited more than 100%. The use of just NaCl produced a higher residual activity than combination of bencol (benzyldimethyl ammoniumchloride) and PG (propyleneglycol).