• 한국수산과학회지
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• 제54권5호
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• pp.617-623
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• 2021

The study was conducted to investigate the dietary supplementation of citrus by-product (CBP) and CBP fermented with Bacillus subtilis (BS) or B. pumilus (BP) on growth performance, feed utilization, innate immune responses and disease resistance of Korean rockfish Sebastes schlegelii. Triplicate groups of juvenile fish (initial weight of 22.9 g) were fed one of five experimental diets which were formulated to replace a synthetic vitamin C (L-ascorbyl-2-polyphosphate, LAPP) with CBP or fermented CBP. A basal diet without LAPP and CBP was used as the control diet (Con). At the end of the 13 weeks feeding trial, growth performance, feed utilization and survival of fish fed the Con were significantly lower than those of fish fed the LAPP, CBP and fermented CBP diets. Fish fed the Con were significantly lower in lysozyme activity than fish fed LAPP, CBP and fermented CBP diets. In a challenge test against Edwardsiella tarda, cumulative mortality of fish fed LAPP, CBP and fermented CBP diets was significantly lower (20-30%) than that of the Con (60%). The findings in this study indicate that the CBP and/or fermented CBP can be a promising vitamin C source for LAPP replacement in diet for Korean rockfish.

• 한국유기농업학회지
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• 제20권1호
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• pp.59-70
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• 2012

본 실험은 다양한 유기질 퇴비의 시용이 Sorghum${\times}$Sudangrass hybrid의 생산성, 사료가치 및 가축사육능력에 미치는 영향을 검토하였고, 그 결과를 요약하면 다음과 같다. Sorghum${\times}$Sudangrass hybrid의 건물수량은 FPM이 19.60ton/ha로 가장 많았으나, PBC와의 유의한 차이는 없었다. 상대사료가치는 1회 예취에서 PBC가 80.66으로 유의하게 높았으나, 2회 예취에서는 FCM을 제외한 모든 시험구에서 유의차를 보이지 않았다. 가축사육능력은 $K_{CP}$와 $K_{TDN}$의 평균값으로 비교해 보았을 때, FPM이 9.45두/ha/yr로서 가장 높은 값을 보였다. 따라서 Sorghum${\times}$Sudangrass hybrid의 유기 재배 시 FPM이 생산성, 사료가치 및 가축사육능력에 있어서 다른 유기질 퇴비에 비해 좋으며, 유기질 퇴비의 연용 시에는 다른 가축분과의 혼용을 통하여 계분의 부족한 유기물을 충족시켜 준다면 작물 및 토양에 있어서 더욱 좋을 것으로 판단된다. 또한 적절한 혼합 비율에 대한 추가적인 연구가 필요할 것으로 판단된다.

• 한국환경농학회지
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• 제20권2호
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• pp.122-126
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• 2001

MS균을 이용하여 고온숙성에 의해 남은 음식물을 양돈사료로 제조하여 양돈 배합사료의 대체효과 및 사료효율에 미치는 영향을 조사하기 위하여 배합사료 100% 급여 (대조구), 남은 음식물 발효사료 대체율을 배합사료 대비 25%, 50% 및 100% 대체 급여구, 4처리로 설정하여 5반복으로 사양시험을 실시하였다. 남은 음식물 발효사료의 전 시험기간 동안 (10 주령부터 27주령까지) 일당 중체량은 배합사료 100% 급여한 대조구에 비해 남은 음식물 발효사료를 25% 및 50% 대체 했을 때 29.4% 및 13.9%로 높았고, 100% 대체구는 38.2% 감소하였다. 일일사료 섭취량, 사료요구율에 있어서 남은 음식물 발효사료를 50% 대체구에서는 대조구에 비해 다소 떨어지지만 유의적인 차이는 보이지 않아 배합사료를 남은 음식물 발효사료로 대체할 수 있는 수준인 것으로 나타났다. 그러나 100% 대체구에서는 대조구에 비해 유의적으로 차이가 있어 돼지 성장에 문제가 있는 것으로 평가되었다. 1 kg당 증체에 요구되는 사료비는 배합사료만 급여한 대조구에서 690.4원인데 비하여 25% 및 50% 남은 음식물 발효사료 대체구에서는 총 증체량이 대조구보다 높게 유지되면서 28.6% 및 49.4%가 절감되는 높은 경제성을 확인할 수 있었다. 100% 남은 음식물 발효사료 급여구에서는 kg 증체에 요구되는 사료비는 약 32.6% 감소되었으나, 전 육성기간동안의 총 증체량이 26.5 kg으로 시험 종료시 출하체중에 훨씬 못 미쳐 실제사양에 적용 가능성은 매우 어려운 것으로 나타났다.

• Fisheries and Aquatic Sciences
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• 제12권4호
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• pp.305-310
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• 2009

This study was carried out to evaluate the use of fermented skipjack tuna viscera (FSTV) as an alternative for fish meal in juvenile olive flounder (Paralichthys olivaceus) diets. Lactobacillus bulgaricus was used as a starter for fermentation of skipjack tuna viscera. Four isonitrogenous (49% crude protein) and isocaloric (4 kcal/g DM) diets were formulated to contain graded levels (0, 5, 10, and 15%) of FSTV. Each experimental diet was fed three replicate groups (40 fish per tank) of juvenile flounder (average weight, $3.3\pm0.2$ g) for 5 weeks. At the end of feeding experiment, inclusion of FSTV up to 15% in diets did not affect survival rate (%) and weight gain of fish. Feed efficiency, protein efficiency ratio, protein and lipid retentions of fish fed the diet containing 10% FSTV were higher than those of fish fed the control diet (P<0.05). The values of fish fed the diet containing 15% FSTV were not different from those of fish fed other diets. Whole body lipid content of fish fed the diet containing 10% FSTV was higher than that of fish fed the diet containing 15% FSTV and control diet. The present results indicate that fermented skipjack tuna viscera could partially replace fish meal in juvenile flounder feed, and the inclusion of 10% FSTV may be efficient in improving the feed utilization of fish.

• 농업생명과학연구
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• 제46권4호
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• pp.119-127
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• 2012

To investigate the influence of multi-probiotic, fermented ginseng byproduct and fermented sulfone on the performance, intestinal microflora and immunity of broiler, a five weeks trial was conducted with 340, 1-d-old $Ross{\times}Ross$ broiler. All broilers were divided into five different groups having 68 birds in each treatment, and they were assigned as control, antibiotic avilamycin (AB), multi-probiotic (MP), fermented sulfone (FS) and fermented ginseng byproduct (FGB). Each artificial or naturally derived probiotic was inoculated 0.1% level with the basal diet, and all diets were provided to birds for five weeks. Weight gain and feed intake were measured weekly basis, and blood, spleen and feces were collectedand used for the physiological properties of broiler chickens. All performances and cholesterol profiles were not significantly differed but numerically lower level of neutral fat and LDL was found in multi-probiotics and FGB treatments respectively. The salmonella spp and E. coli numbers in the ileum were high in control in relation to those of other treatments and were significantly decreased in antibiotics treatments (p<0.05). In addition, Lactobacillus spp. showed significantly higher proliferation in MP as compared to that of others (p<0.05). Fecal ammonia and $CO_2$ gas emission was significantly decreased in MP, FGB and FS, respectively (p<0.05), but significantly increased proliferation of spleen was determined in MP group in comparison of other treatments (p<0.05). Therefore, the results indicates that multi-probiotics would be valuable feed additives to improve the salmonella, E. coli and Lactobacillus proliferation, and manure gas emission of broiler chickens, but further study related to the production of manure gas emission of MP is necessary.

• 한국가금학회지
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• 제25권3호
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• pp.147-155
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• 1998

Dietary levels of fermented poultry manure(FPM) 0%, 5%, 10% and 20% were included in experimental diets. It is to examine the effects of diets containing different levels of FPM on the performance of broiler chicks, including the nutrient availabilities, compared with that of the commercial diet. In order to evaluate the nutritive value of FPM, feeding and metabolism trials were conducted with a total of 160 broiler chicks for a period of 4 weeks. Contents of CP, crude fat, crude ash, crude fiber and NFE of FPM were 19.43, 2.43, 15.44, 16.57 and 38.31 %, respectively. Although there were no statistical difference in the body weight gain among treatments, weight gain achieved by the group fed diets of 5% substitution of FPM was slightly better than others. The amount of feed intake was significantly higher for FPM included groups than for control group, especially in the group fed diets of 10% substitution of FPM. The feed efficiency tended to be better for the control group than the FPM groups. The feed efficiency tended to get worse as the levels of FPM increased. The digestibility of DM, CP and NFE tended to be similar among treatments, especially it was lower for the group fed diets of 20% substitution of FPM compared with that of another groups. In conclusion, up to 10% substitution of FPM to the diet can be utilized effectively as a broiler feed ingredient, without adversely affecting growth performance.

• Animal Bioscience
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• 제34권2호
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• pp.285-294
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• 2021

Objective: This study was conducted to investigate the supplementary effect of a phytogenic blend (SPA: a mixture containing fermented Schisandra chinensis pomace, fermented Pinus densiflora needle extract, and Allium tuberosum powder in the ratio of 2:2:1) on egg production, egg quality, blood constituents, and visceral organs in laying hens. Methods: A total of 135 Hy-line brown laying hens (48-wk-old) were randomly allocated to three dietary treatments with 5 replicates of 9 hens. The control group (CON) was fed a basal diet (no exogenous SPA addition) and the experimental groups were fed the basal diet containing SPA at the level of 0.1% and 0.3% for 6 weeks. Results: The feed intake was significantly improved in SPA supplemented groups as compared with the control (p<0.05). However, egg production, daily egg mass, and feed conversion ratio were not different among the dietary treatments (p>0.05). For egg quality traits, only Haugh unit (HU) was significantly improved in SPA (0.3%) (p<0.05) as compared with other groups. However, HU was not affected during 4-wk of storage at 18℃ among the dietary treatments (p>0.05). Furthermore, SPA supplementation did not affect the blood biochemical constituents except for the phosphate content, which was significantly higher in SPA groups than the CON group (p<0.05). There were no significant differences in visceral organ characteristics and immune indicators (immunoglobulin A [IgA], IgG, and IgM) in SPA or CON groups. Conclusion: This study suggested that the supplementation of SPA may have beneficial effects on feed intake and egg quality in laying hens.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1287-1293
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• 2016

The objectives of this experiment was to evaluate the subsequent growth and organ weights, blood profiles and cecal microbiota of broiler chicks fed pre-starter diets containing fermented soybean meal products during early phase. A total of nine hundred 1-d-old chicks were randomly assigned into six groups with six replicates of 25 chicks each. The chicks were fed control pre-starter diet with dehulled soybean meal (SBM) or one of five experimental diets containing fermented SBM products (Bacillus fermented SBM [BF-SBM], yeast by product and Bacillus fermented SBM [YBF-SBM]; Lactobacillus fermented SBM 1 [LF-SBM 1]; Lactobacillus fermented SBM 2 [LF-SBM 2]) or soy protein concentrate (SPC) for 7 d after hatching, followed by 4 wk feeding of commercial diets without fermented SBMs or SPC. The fermented SBMs and SPC were substituted at the expense of dehulled SBM at 3% level on fresh weight basis. The body weight (BW) during the starter period was not affected by dietary treatments, but BW at 14 d onwards was significantly higher (p<0.05) in chicks that had been fed BF-SBM and YBF-SBM during the early phase compared with the control group. The feed intake during grower and finisher phases was not affected (p>0.05) by dietary treatments. During total rearing period, the daily weight gains in six groups were 52.0 (control), 57.7 (BF-SBM), 58.5 (YBF-SBM), 52.0 (LF-SBM 1), 56.7 (LF-SBM 2), and 53.3 g/d (SPC), respectively. The daily weight gain in chicks fed diet containing BF-SBM, YBF-SBM, and LF-SBM 2 were significantly higher values (p<0.001) than that of the control group. Chicks fed BF-SBM, YBF-SBM, and LF-SBM 2 had significantly lower (p<0.01) feed conversion ratio compared with the control group. There were no significant differences in the relative weight of various organs and blood profiles among groups. Cecal microbiota was altered by dietary treatments. At 35 d, chicks fed on the pre-starter diets containing BF-SBM and YBF-SBM had significantly increased (p<0.001) lactic acid bacteria, but lowered Coli-form bacteria in cecal contents compared with those fed the control diet. The number of Bacillus spp. was higher (p<0.001) in all groups except for LF-SBM 1 compared with control diet-fed chicks. At 7 d, jejunal villi were significantly lengthened (p<0.001) in chicks fed the fermented SBMs vs control diet. Collectively, the results indicate that feeding of fermented SBMs during early phase are beneficial to the subsequent growth performance in broiler chicks. BF-SBM and YBF-SBM showed superior overall growth performance as compared with unfermented SBM and SPC.

• 한국환경농학회지
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• 제19권4호
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• pp.332-338
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• 2000

본 연구는 대조구로서 일반배합사료에서 사육된 청둥오리와 처리구로서 일반배합사료에 MS 발효 잔반사료를 20% 또는 50%를 혼합하여 일정기간 사육한 후 사육효과를 알아보았고 이화학적 분석 및 아미노산과 지방산 분석 등을 통해 처리구에 따른 육질을 비교함으로서 MS 발효 잔반사료의 오리사료로서의 효율성을 검토하였다. 사육효과에 대한 결과로서 도체율 및 각 장기무게는 대조구와 처리구간에 유의적 차이가 발생되지 않았으나, 사료효율에 있어서는 처리구보다 대조구에서 좋은 결과를 보여주었다 (P<0.05). 이화학적 분석으로서 보수성과 pH는 모든 처리구간에 있어서 유의차가 없었다. 그러나 육색에 대한 결과를 보면 명도($L{\ast}$)는 대조구가 20% 또는 50% MS 처리구보다 높은 값을 나타냈고 (P<0.05), 적색도($a{\ast}$)에서는 각 처리구가 대조구보다 더 높게 나타났다 (p<0.05). 관능평가로서 냄새와 기호성에서 있어서는 각 처리구간 유의적 차이가 없었다 (p>0.05). 한편 각 처리구의 부위별 아미노산 분석에 있어서 다리고기의 경우는 20% MS 처리구가 가장 많은 함량을 가지는 것으로 분석되었고, 가슴고기에 있어서는 대조구가 20% 또는 50% MS 처리구보다 더 높게 나타났다. 지방산 분석에 있어서 각 처리구별 불포화지방산 함량은 대조구보다 MS 발효 잔반사료 처리구에 더 많은 함량이 함유된 것으로 분석되었다. 이와같은 결과를 종합해 볼 때 청둥오리 사육에 있어서 시판 배합사료에 50% 수준까지 MS 발효 배합사료를 혼합급여하여도 성장 및 육질에 큰 영향을 주지 않음을 알 수 있었다. 비록 사료효율 측면에서 시판 배합사료에 비해 MS 발효 잔반사료 처리구가 더 낮게 나타났으나, 음식물쓰레기로 인한 경제적 및 환경적 손실을 감안할 때 본 연구결과가 주는 의미는 크다고 여겨진다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.862-870
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• 2014

This study was conducted to investigate the effects of brown seaweed (Undaria pinnatifida) by-product and seaweed fusiforme (Hizikia fusiformis) by-product supplementation on growth performance and blood profiles including serum immunoglobulin (Ig) in broilers. Fermentation of seaweeds was conducted by Bacillus subtilis and Aspergillus oryzae. In a 5-wk feeding trial, 750 one-d-old broiler chicks were divided into 5 groups, and were assigned to the control diet or experimental diets including control+0.5% brown seaweed (BS) by-product, control+0.5% seaweed fusiforme (SF) by-product, control+0.5% fermented brown seaweed (FBS) by-product, and control+0.5% fermented seaweed fusiforme (FSF) by-product. As a consequence, body weight gain (BWG) and gain:feed of seaweed by-product groups were clearly higher, when compared to those of control diet group from d 18 to 35 and the entire experimental period (p<0.05). In mortality rate, seaweed by-product groups were significantly lower when compared to control diet group during entire experimental period (p<0.05). However, Feed Intake of experimental diets group was not different from that of the control group during the entire experimental period. Whereas, Feed Intake of fermented seaweed by-product groups was lower than that of non-fermented seaweed groups (p<0.05). Total organ weights, lipids, and glutamic oxalacetic transaminase (GOT) of all treatment groups were not different from those of control group. However, glutamic pyruvate transaminase (GPT) of all treatment groups was higher than that of control group at d 17 (p<0.05). In case of serum Igs concentration, the concentration of IgA antibody in BS, SF, FSF treatment groups was significantly higher than in control group at d 35 (p<0.01). IgA concentration in FBS supplementation groups was negligibly decreased when compared to the control group. IgM concentration in the serums of all treatment groups was significantly higher than in control group (p<0.05) and in fermented seaweed by-product groups were much higher than in non-fermented seaweed groups (p<0.05). On the other hand, IgG concentrations in all treatment groups were lower than in control group (p<0.05). Taken together, our results suggest that by-product dietary supplementation of BS, SF, FBS, and FSF in poultry may provide positive effects of growth performance and immune response.