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A Comparative Study on the Electrophysiological Properties of Medial and Lateral Spinoreticular Tract Cells in Cats (고양이의 내측 및 외측 척수망상로 세포의 전기생리학적 비교연구)

  • Lee, Suk-Ho;Jun, Jae-Yeol;Park, Choon-Ok;Goo, Yong-Sook;Kim, Jun;Sung, Ho-Kyung
    • The Korean Journal of Physiology
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    • v.24 no.1
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    • pp.181-194
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    • 1990
  • Antidromically activated spinoreticular tract (SRT) cell units in the lumbosacral enlargement of ${\alpha}-chloralose$ anesthetized cats were classified as medial and lateral SRT units according to the location of their axonal termination. Identified SRT units were tested fer antidromic conduction velocity, laterality of their axonal projection, the location in spinal gray, peripheral receptive field, the response pattern to graded mechanichal stimulation and the responsiveness to $A{\delta}$ and C volley of the peripheral nerve. 1) The 59% of 34 medial SRT units were recorded in ipsilateral side to the antidromic stimulation site, but 60% of the 47 lateral SRT units projected to contralateral side. 2) Most of the medial SRT cells and rostral ventrolateral medulla (RVLM)-projecting lateral SRT cells were recorded in lamina VII & VIII. The LRN (lateral reticular nucleus)-projecting SRT cells, however, distributed through all the laminae except superficial ones (I & II). 3) The identified SRT units were classified as low theshold (LT), deep, high threshold (HT), wide dynamic range (WDR) cells, based on the response patterns to graded mechanical stimuli. The proportion of SRT units which receive noxious input was 37.5%, 25% and 75% in the medial, LRN-projecting and RVLM SRT group, respectively. 4) There was no significant difference in the mean conduction velocities between the 3 groups. But the deep cells had significantly higher velocity than that of the HT cells. The above results show that the peripheral inputs to the SRT units are different in the 3 groups: medial, LRN & RVLM SRT group. Especially in case of the SRT cells projecting to RVLM which is a probable candidate fur the integration center of various pressor reflexes such as somatosympathetic reflex, the noxious informations occupy higher proportion of input to them than in other groups. Therefore the noxious information transmitted through the lateral SRT destined for RVLM is expected to play a role in somatosymapthetic reflex.

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Arthroscopic Treatment for Residual Pain after Ankle Fracture (족근관절 골절 치료후 발생한 동통에 대한 관절경의 이용)

  • Lee Beom Koo;Park Hong Kee;Seong In Ho;Kim Keon Beom;Jang Young hun;Choi Jang Seok
    • Journal of the Korean Arthroscopy Society
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    • v.4 no.1
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    • pp.61-66
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    • 2000
  • Purpose : The diagnosis of the causes of residual pain after ankle fractures and the treatment is not simple. The authors analyzed the clinical results of the patients with residual pain after ankle fracture fur whom ankle arthroscopy was undertaken, for the purpose of evaluating the efficacy of arthroscopic diagnosis and treatment. Materials and Methods : From January 1997 to June 1998, ankle arthroscopy was done for the seventeen patients suffering from residual ankle pain and limitation of motion after ankle fracture. Their symptoms were not improved despite conservative treatment. There were sixteen men and one woman, and their mean age was 37.4 years. Result : Radiological examination revealed loose bodies in two patients, and osteophytes at the anterior rim of the tibia in fourteen patients. By the classification of osteoarthritic change, three ankles were assigned to grade 0, eight to grade I, six to grade II, and none to grade III. On arthroscopic examination, soft tissue impingement was found In thirteen cases, loose bodies in five cases, and osteochondral lesions in four cases. For arthroscopic treatments, removal of soft tissue, abrasion of osteophytes, cartilage shaving and removal of loose bodies were done. Clinical results were evaluated using Evanski and Waugh score. Preoperative overall score of 58.7 points jumped to 74.6 points after the operation, and in fourteen patients$(82\%)$ the symptoms were improved. Conclusion : Ankle arthroscopy is considered to be a very useful diagnostic and treatment method fer the patients with residual complaints after ankle fracture.

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A Study on the Bioavailability of Organic Ca in Growing Rats (성장기 흰쥐에서 유기태 칼슘의 체내 이용성 연구)

  • Park, Mi-Na;Cho, Su-Jung;Kim, Hee-Kyong;Kim, Jae-Hong;Kim, Min-Ho;Kim, Wan-Sik;Lee, Yeon-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.1
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    • pp.87-94
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    • 2012
  • We evaluated the bioavailability of a novel organic Ca supplement chelated with milk protein (CaMP) in growing rats and compared it with those of Ca carbonate and Ca citrate. Four-week-old male rats were divided into six groups (n=6/group) and fed AIN-93G-based experimental diets containing three Ca sources, Ca carbonate, Ca citrate, and CaMP at two levels, normal (0.5%, w/w) and high (1.5%, w/w), for 6 weeks. Growth, mineral contents of serum, Ca content and breaking force of femur, and Ca absorption were measured. There were no significant differences in weight gain or food intake, but food efficiency ratio (FER) of CaMP in the high Ca group was higher than those of other groups. Ca and P concentrations in serum were within normal range in all experimental rats. There was no difference in Ca content of the femur among all of the groups. Although there was no statistical significance in bone breaking force of the femur among the groups, the CaMP groups had a higher breaking force compared to other groups. Further, Ca absorption rate significantly increased in the CaMP groups (p<0.05). These results demonstrate that the CaMP supplement did not exhibit any negative effect on growth or Ca metabolism of the rats. Therefore, CaMP can be recommended as a good Ca supplement with regard to bone metabolism and Ca bioavailability.

Development of Digital Games Based on Historical Material and its Design Components - With History Based Games of 5 Countries (역사소재 기반 디지털게임의 발전과정 및 기획요소 연구 - 동.서양 5개국의 역사소재 게임을 중심으로)

  • Moon, Man-Ki;Kim, Tae-Yong
    • Journal of Broadcast Engineering
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    • v.12 no.5
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    • pp.460-479
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    • 2007
  • When culture took large part in industrial area, every country has tried to utilize own cultural contents for educational or commercial purpose and the various cultures and histories are recognized as a main concept or subject so that a number of scholars who study history increase. In video game field, special characteristics of interface that audiences participate in the game to complete story-telling is considered as efficient material for learning process. As observed above, it is important to analyze the games that every country makes and export to the world in which the video games is understood as a play of human in general. This Paper has firstly analyzed the most favorite historical games developed in Korea, the USA, Japan, Taiwan and Germany from 1980 to 2005 and secondly, compared that wars and historical origin appears in game scenario, a world view and background story and finally after point out the preferable era and genre of the countries then propose the promising way of design for historical video games. In the process of analysis of a view and heroes in historical games, we compared the real persons, the real historical events and novel in which 11.8% only employed the real persons in 8 out of 68 games. Also the real history and background story are appeared in 37 games which is 54.4% of them. We discovered that the main material that is popular for each country is the historical backing rather than real persons where the favorite historical background is chosen at which they are proud of; 3-Throne era with strong ancient Gogurye for Korea, the 1st and 2nd World Wars and the Independence War for the USA, the tide of war around Middle age for Japan, ancient history of Europe for Germany. The favorite age for video games is Ancient times with 37 games for 54.4%, Middle Age with 7 games fer 10.3%, the prehistoric age with 5 games for 7.35%, remote age with 1 for 1.47%, while current historical games favor Ancient or Modern Age.

Studies on the Response of Isogenic lines of Compact and lax Type to Fertilization and Plant Density in Malting Barley (맥주맥에 있어서 직두형 및 수두형 Isogenic line의 시비량 및 재식밀도에 대한 반응)

  • Chang-Hwan Cho;Eun-Sup Lee;Je-Cheon Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.3
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    • pp.366-374
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    • 1986
  • Taiwan land race, Taichung 2 rowed barley #1 was crossed with England cultivar, Deba abed in 1972, and two isogenic lines-lax and compact heads-were made in 1982. So as to three fertilizer and three seeding density levels were treated at Dan-Kook Agricultural College farm located in Cheonan in 1984-1985. Lax head type had longer spike length and rachis than compact type. The traits uninfluenced by different head types were heading time, number of grains per spike, yield and soluble nitrogen content. However, the traits related to malting quality such as 1,000 grain weight, assortment ratio, protein content of grain and malt, malt extract, malt total nitrogen content, Kolbach index and diastatic power were significantly affected by head types. The number of spikes per m2, yield, assortment ratio, crude protein content of grain and malt yield ratio, extract yield ratio, malt total nitrogen, souble nitrogen content, Kolbach index and diastatic power were affected by fertilizer and seeding density levels in both isogenic lines. The malting quality was best at 12-12-9 kg/l0a fertilizer level and the yield was greatest at 15-12-9 kg/l0a level. Narrow-spaced or drilling seeding had greater yield and better malting quality than conventional row seeding. The plant with lax head type had some advantages in malting quality, suggesting that this trait would be considered as an selection criterion fer good malting quality lines.

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Effect of Feeding Lactobacillus reuteri to Broiler on Growing Performance, Intestinal Microflora and Environmental Factor (lactobacillus reuteri의 급여가 육계의 성장 특성, 장내미생물 변화, 혈청 성상 및 사육환경에 미치는 영향)

  • 김상호;박수영;이상진;류경선
    • Korean Journal of Poultry Science
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    • v.30 no.1
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    • pp.17-28
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    • 2003
  • A feeding trial was carried out to evaluate the effects of feeding Lactobacillus reuteri culture(LRC) on the performance, nutrients digestibility, intestinal microflora, serum metabolites, ammonia generation and litter dampness in broiler chicks. Four hundred eighty, one day old male chicks were fed into none, 0.1, 0.2 and 0.4% of LRC supplementation fer seven weeks. Basal diets excluded antibiotics contained ME 3,100, 3,100 kcal/kg, and CP 22.0, 20.0% for starter and grower, respectively. Weight gain of chicks fed LRC was significantly higher than no supplemental group in overall period(P<0.05). Feed intake was the highest in the 0.1% LRC, but not statistically different from other treatments. Feed conversion showed no significance among treatments. Viable Lactobacillus spp. number of chicks fed 0.2 and 0.4% LRC was significantly higher in cecum at seven weeks of age compared to the none(P<0.05). The tendency of anaerobes number was similar to Lactobacillus spp in ileum and cecum. Total number of E. coli and Salmonella were no difference in all treatments. In serum metabolites, feeding LRC increased triglyceride, and inorganic phosphorus, but no different total protein, albumin, total cholesterol, glucose, blood urea nitrogen and Ca. Nutrients digestibility improved significantly in 0.4% LRC compared to that of none(P<0.05). Fecal NH$_3$, gas generation was greatly decreased in the LRC supplemental groups(P<0.05). Moisture contents of bedding was also significantly decreased in LRC feeding group. It was concluded from the present study that feeding Lactobacillus reuteri culture improved the growth performance and nutrients digestibility of broiler chicks and minimize the fecal noxious gas emission.

Studies on the method of bring to perfectly from defective cutting Sapling (삽목재육묘의 육함에 관한 연구)

  • 김문협
    • Journal of Sericultural and Entomological Science
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    • no.11
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    • pp.31-44
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    • 1970
  • This experiment is conducted fer method cf bring to perfectly from defective cutting sapling 25 a production method of sapling which is to substitute for conventional graf ting. 1. In green wood cutting root comes out after 15 days of cutting with nearly straight development of root system; after 40 days of cutting, roots with total length of 1119 mms 43 roots, and 5.4 grs a root in total weight obtained. 2. Survival percentage of green wood cutting ranges between 56% and 90%, average 73% of that and it varies with natural characteristics of varietes. The results show variety of Gaeryang-Suban and Iljiroe with 80% as a best ones in contrast with the variety of Shipyung. 3. The varietics or Gaeryang-Suban. Iljiroe, Suwon-Sang No.4, Rosang makes of much more roots than Yongchonchuwoo and Shipyung do. 4. Root ability made good number of roots commercially, when cutting is conducted soaking in 0.01 % NAA solution or 0.02% NAA solution for 2 or 3 seconds as a chemical promoter. 5. Economical measures for increase of scion adapted 1) 2 scions from a green wood 50 cm long should be taken and basal pan of scion at its middle portion should be cut right under the node. 2) Scions below 50cm long may be used. Small scions or growthceased shoots h3ve also considerable root ability enough to be used. 3) Thus far, up 100,000 scions might be produced in 10 a. 6. We can find number of root increased, when cutting the opposite side in obliquity manner at 450. 7. When 110,000 saplings in 10 a. for the production of bring to perfectly from defective cutting sapling planted, quality of stocks does not go to bad condition without any obstacles in practical use. 8. Although the times or grafting delayed until middle of July, quality of sapling goes just a little down. Grafting may be conducted from early June to middle of July separately in several times, and the green wood of prunned mulberry in spring is available for a scion after end of June. 9. 10 grs weight of defective cutting sapling makes 95% of complete sapling, otherwise 5 or 10 grs in weight of one becomes 80% of complete sapling with its quality as similar as grafted one. 10. When the sapling planted, its branches should be cut, leaving 3 or 4 buds at the bottom of new branches. 11. In view of economical stand point, production cost of bring to perfectly from defective cutting sapling obtains 52% of grafting cost.

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Assessment of DNA damage and Chromosome aberration in human lymphocyte exposed to low dose radiation detected by FISH(fluorescence in situ hybridization) and SCGE(single cell gel electrophoresis) (FISH기법 및 단세포전기영동기법을 이용한 저선량 방사선에 의한 DNA 상해 및 염색체이상 평가)

  • Chung, Hai-Won;Kim, Su-Young;Kim, Byung-Mo;Kim, Sun-Jin;Kim, Tae-Hwan;Cho, Chul-Koo;Ha, Sung-Whan
    • Journal of Radiation Protection and Research
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    • v.25 no.4
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    • pp.223-232
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    • 2000
  • Comparative study was performed for the assessment of DNA damage and Chromosomal aberration in human lymphocyte exposed to low dose radiation using fluorescence in situ hybridization(FISH) and single cell gel electrophoresis(SCGE). Chromosomal aberrations in human lymphocytes exposed to radiation at doses of 5, 10, 30 and 50cGy were analysed with whole chromosome-specific probes by human chromosome 1, 2 and 4 according to PAINT system. FISH with chromosome-specific probe has been used to be a valid and rapid method fer detection of chromosome rearrangements induced by low dose radiation. The frequencies of stable translocation per cell equivalents were 0.0116, 0.0375, 0.040f, 0.0727 and 0.0814 for 0, 5, 10, 30 and 50cGy, respectively, and those of dicentric were 0.00, 0.0125, 0.174, 0.0291 and 0.0407 respectively. Radiation induced DNA damage in human lymphocyte in a dose-dependent manner at low doses from 5cGy to 50cGy, which were analysed by single tell gel electrophoresis(SCGE). From above results, FISH seemed to be useful for radiation biodosimetry by which the frequencies of stable aberrations in human lymphocyte can be observed more easily than by conventional method and SCGE also seemed to be sensitive method f9r detecting DNA damage by low dose radiation exposure, so that those methods will improve our technique to perform meaningful biodosimetry for radiation at low doses.

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Cholesterol-lowering and Anti-obesity Effects of Polymnia Sonchifolia Poepp. & Endl. Powder in Rats Fed a High Fat-High Cholesterol Diet (야콘 분말이 고지방-고콜레스테롤 식이를 급여한 흰쥐의 콜레스테롤 저하 및 비만 억제효과에 미치는 영향)

  • Kim, Ah-Ra;Lee, Jae-Joon;Lee, Yu-Mi;Jung, Hae-Ok;Lee, Myung-Yul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.2
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    • pp.210-218
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    • 2010
  • This study was carried out to investigate the effect of Yacon (Polymnia sonchifolia Poepp. & Endl.) powder on cholesterol-lowering and anti-obesity effects in rats fed a high fat-high cholesterol diet for 4 weeks to induce hyperlipidemic and obese rat model. Weight-matched male Sprague-Dawley rats were assigned to four groups according to dietary fat, cholesterol levels and Yacon powder levels. Experimental groups were normal diet group (N), high fat-high cholesterol diet group (HFC), high fat-high cholesterol diet with 5% Yacon powder group (HFC-PSL) and high fat-high cholesterol diet with 10% Yacon powder group (HFC-PSH). The body weight gain and FER were increased by a high fat-high cholesterol diet, but gradually decreased in the Yacon powder fed groups compared with the HFC group. Food intake was lower in HFC groups compared with N group. The liver and adipose tissue weights of HFC group were heavier than those of N group, whereas those of groups administered Yacon powder were gradually decreased. The serum ALT, AST, ALP and LDH activities elevated by a high fat-high cholesterol diet were significantly decreased by Yacon powder administration. Levels of serum total cholesterol, LDL-cholesterol, atherogenic index and cardiac risk factor showed a decreasing tendency in the Yacon powder fed groups compared with HFC group. The serum HDL-cholesterol level decreased in the HFC group and markedly increased in the Yacon powder fed groups. Levels of total cholesterol and triglyceride in liver and adipose tissues were lower in Yacon powder administered groups than those in HFC group. These results suggest that Yacon powder may improve lipid metabolism of serum, liver, and adipose tissue and potentially reduce lipid storage.

Effects of Peucedanum japonicum Thunb. on Lipid Metabolism and Antioxidative Activities in Rats Fed a High-Fat/High-Cholesterol Diet (갯기름나물이 고지방-고콜레스테롤 식이를 급여한 흰쥐의 지질대사 및 항산화 활성에 미치는 영향)

  • Son, Hee-Kyoung;Kang, Su-Tae;Lee, Jae-Joon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.5
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    • pp.641-649
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    • 2014
  • This study was carried out to investigate the effects of Peucedanum japonicum Thunb. (PJT) powder on lipid metabolism and antioxidative activity in rats fed a high-fat/high-cholesterol diet for 4 weeks. Male Sprague-Dawley rats were assigned to four groups: normal diet group (N), high-fat/high-cholesterol group (HFC), high-fat/highcholesterol with 5% PJT powder (HFC-PBL), and high-fat/high-cholesterol with 10% PJT powder (HFC-PBH). Body weight gain and food efficiency ratio (FER) increased in the HFC group, whereas they gradually decreased in the PJT powder-fed groups. Food intake was not significantly different between the experimental groups. Liver and adipose tissue weights of the HFC group were heavier than that of the N group, whereas the groups fed PJT powder showed gradual reduction of tissue weights. Serum alkaline phosphatase (ALP) activity significantly decreased after PJT powder administration. Serum triglyceride level significantly decreased in groups fed PJT powder compared to the HFC group. The serum low density lipoprotein cholesterol (LDL-cholesterol) level of the HFC group increased by 73.70% than that of the N group, whereas serum high density lipoprotein (HDL-cholesterol) tended to decrease in groups fed PJT powder compared to the HFC group. Levels of triglycerides in epididymal and mesenteric adipose tissues, as well as cholesterol levels in liver and adipose tissues were lower in the groups fed 10% PJT powder compared to the HFC group. The liver glutathione (GSH) level increased in the groups fed PJT powder compare to the HFC group. The liver thiobarbituric acid reactive substances (TBARS) content significantly decreased in the groups fed PJT powder compared to the HFC group. These results suggest that PJT powder may improve lipid metabolism in the serum, liver, and adipose tissue and prevent oxidative stress by stimulating antioxidative systems in rats fed a high fat-high cholesterol diet.