• 한국컴퓨터정보학회논문지
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• 제23권9호
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• pp.107-112
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• 2018

Smart livestock has been proposed as a solution to increase farmers' income and new recruitment of livestock farmers. In this paper, a standardization Plan of breeding management device and collected information for smart livestock cattle was proposed. l Sophisticatedly, basic information will be established for all six types of livestock breeding management device: military automatic feeder, calf automatic feeder, smart milk cooler, feed bin to be able to measure feed residue, smart scale, and biometric information collection device. The standardization, common use, and stabilization of major livestock management device and collected information were suggested to solve the problems caused by in existing breeding management device.

• Food Science and Biotechnology
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• 제15권4호
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• pp.506-510
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• 2006

To be used as a source of astaxanthin by animals, the red yeast Xanthophyllomyces dendrorhous needs to be dried and the cell wall ruptured. Spray-drying and flat-roller milling successfully prepared the yeast as a feed additive with little loss of astaxanthin. Spray-drying successfully dried the yeast with negligible decomposition of astaxanthin compared to drum-drying. By repeated milling with a flat-roller mill, astaxanthin extracted with ethanol increased from 0.01 to 1.31 mg astaxanthin/g yeast. This method did not decompose astaxanthin in contrast to chemical digestion of the cell wall. Flat-roller milling effectively flattened and cracked the dried cells. Astaxanthin in yeast prepared by spray-drying and flat-roller milling was well absorbed by animals. Specifically, when spray-dried and milled yeast was supplied in the feed (40 mg astaxanthin/kg feed), astaxanthin was successfully absorbed (1,500 ng/mL blood and 1,100 ng/g skin) by laying hens.

• Journal of Biosystems Engineering
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• 제34권5호
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• pp.342-350
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• 2009

Currently TMR feed produced in commercial plant is one of the major source to feed cattle for both beef and dairy farm. However, because of lack of cutting and mixing system for utilizing domestic produced firmly baled round roughage in commercial TMR plant, these commercial TMR feed can not satisfy to farmers both in quality and price points of view. In order to solve these problems, a farm group size TMR plant model was developed in this study. The model plant was consist of round bale receiving and cutting system, pneumatic conveying system for transfer the roughage which was cut at the cutter to TMR mixer through pneumatic conveyor, TMR mixer enable to soften the stiff rice strew and to mix with other ingredients, finished feed bin which can be transfer to either packing system or individual farm, packing system by tycon bag which contains 400 kg unit and bulk unloading system to individual farmer. Also, a simulation model ARENA was applied to the model system in order to evaluate and check the production rate in each unit process and operation rate of total system and to find out if there are any clogged unit system obstructing the smooth flow of the total process flow. Processing cycle for produce one batch of the model plant was less than 30 minutes. Thus, it will take less than four hours for producing 16 tons per day equivalent to 1,000 beef cattle's daily feed.

• 한국초지조사료학회지
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• 제42권1호
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• pp.47-53
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• 2022

The objective of this study was to verify the effect of pig slurry application with acidification and biochar on feed value, nitrogen use efficiency (NUE) of maize forage, and ammonia (NH₃) emission. The four treatments were applied: 1) non-pig slurry (only water as a control, C), 2) only pig slurry application (P), 3) acidified pig slurry application (AP), 4) acidified pig slurry application with biochar (APB). The pig slurry and biochar were applied at a rate of 150 kg N ha^-1 and 300 kg ha^-1, respectively. The AP and APB treatments enhanced all feed values compared to C and P treatments. The NUE for plant N was significantly increased 92.1% by AP and APB treatment, respectively, compared to the P treatment. On the other hand, feed values were not significantly different between AP and APB treatments. The acidification treatment with/without biochar significantly mitigated NH₃ emission compared to the P treatment. The cumulative NH₃ emission throughout the period of measurement decreased by 71.4% and 74.8% in the AP and APB treatments. Also, APB treatment reduced ammonia emission by 11.9% compared to AP treatment. The present study clearly showed that acidification and biochar can reduce ammonia emission from pig slurry application, and pig slurry application with acidification and biochar exhibited potential effects in feed value, NUE, and reducing N losses from pig slurry application through reduction of NH₃ emission.

• 미생물학회지
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• 제50권4호
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• pp.345-350
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• 2014

여름철의 고온으로의 온도 상승에 따른 사료빈 내의 생균제 L. plantarum, S. cerevisiae 및 B. subtilis의 생존성을 실험실의 가상 조건에서 분석하였다. 상온인 $25^{\circ}C$에서 멸균 사료와 비멸균 사료에 단일 혹은 혼합 균주 첨가 시 pH 변화와 생균제들의 생장을 상호 비교하였다. pH는 멸균 사료와 비멸균 사료 모두에서 4일째에 가장 감소한 것은 같았으나 비멸균 사료에서는 2일째까지는 상승하는 변화의 양상을 보여주었다. 멸균 여부 혹은 혼합 여부와 관계없이 S. cerevisiae와 B. subtilis의 생균수는 일정하게 유지되었지만 L. plantarum의 경우에는 그 수가 모두 줄어드는 것을 확인할 수 있었다. 따라서 3종의 혼합 생균제는 상호 길항작용은 없는 것으로 나타났다. 멸균 및 비멸균 사료에 생균제를 첨가한 후 $60^{\circ}C$의 고온 환경에서 사료의 pH 와 단일접종 생균제의 생존성을 조사하였다. 멸균 및 비멸균 사료 사이에 뚜렷한 pH의 변화는 관찰되지 않았으며 B. subtilis의 pH가 가장 낮게 관찰되었다. 고온 하에서 L. plantarum과 S. cerevisiae 균주는 생존할 수 없었으며, 내열성을 가진 B. subtilis 균주는 생존하면서 사료에 자연적으로 생존하는 오염세균의 증식을 억제하였다. 또 B. subtilis를 접종한 비멸균 사료에서 2일째부터 오염 곰팡이가 관찰되지 않았다. 따라서 내열성이 강한 B. subtilis 균주를 사용하면, 여름철 사료빈 내에 병원성 세균과 곰팡이의 오염을 억제할 수 있는 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제17권1호
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• pp.58-66
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• 2007

Isolation, expression, and characterization of a novel $endo-{\beta}-1,3(4)-D-glucanase$ with high specific activity and homology to Bacillus lichenases is described. One clone was screened from a genomic library of Paenibacillus sp. F-40, using lichenan-containing plates. The nucleotide sequence of the clone contains an ORF consisting of 717 nucleotides, encoding a ${\beta}-glucanase$ protein of 238 amino acids and 26 residues of a putative signal peptide at its N-terminus. The amino acid sequence showed the highest similarity of 87% to other ${\beta}-1,3-1,4-glucanases$ of Bacillus. The gene fragment Bg1 containing the mature glucanase protein was expressed in Pichia pastoris at high expression level in a 3-1 high-cell-density fermenter. The purified recombinant enzyme Bg1 showed activity against barley ${\beta}-glucan$, lichenan, and laminarin. The gene encodes an $endo-{\beta}-1,3(4)-D-glucanase$ (E. C. 3.2.1.6). When lichenan was used as substrate, the optimal pH was 6.5, and the optimal temperature was $60^{\circ}C$. The $K_m,\;V_{max},\;and\;k_{cat}$ values for lichenan are 2.96mg/ml, $6,951{\mu}mol/min{\cdot}mg,\;and\;3,131s^{-1}$, respectively. For barley ${\beta}-glucan$ the values are 3.73mg/ml, $8,939{\mu}mol/min{\cdot}mg,\;and\;4,026s^{-1}$, respectively. The recombinant Bg1 had resistance to pepsin and trypsin. Other features of recombinant Bg1 including temperature and pH stability, and sensitivity to some metal ions and chemical reagents were also characterized.

• 한국축산식품학회지
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• 제38권3호
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• pp.629-635
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• 2018

The present study investigated the effects of feed form and distiller's dried grain with solubles (DDGS) on meat quality and fatty acids profile of broiler chickens. A total of 720 broilers (Ross 308; average BW [body weight] $541{\pm}5.7g$) were randomly allotted to six treatments. Birds were fed three different feed forms (mash; SP, simple pellet; EP, expanded pellet) and DDGS (0 or $200g\;kg^{-1}$) in a $3{\times}2$ factorial arrangement. The addition of DDGS and EP to the diet resulted in increased shear force of breast meat. Moreover, DDGS inclusion in the diet reduced the concentration of stearic acid and behenic acid in thigh meat. Pelleting (SP and EP) of the diets increased palmitic acid content in the thigh, whereas the linolenic acid content decreased. The breast mass was higher with EP and SP diets than with the mash diet. Feed processing led to increased pectoralis muscle and drum mass compared to mash-fed chickens. In conclusion, our results demonstrated that EP decreased thigh linolenic acid and meat shear force. In addition, DDGS supplementation in broiler hampers meat quality by increasing the shear force.

• 한국분무공학회지
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• 제12권1호
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• pp.1-10
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• 2007

The low frequency combustion instability phenomena generated by pressure drop oscillation such as propellant shake in feed line are studied. To generate the flowrate oscillation by the pressure pulsation up to 400Hz without flow discontinuities and cavitations, a hydrodynamic mechanical pulsator of rotating disk type was produced. Injection pressure conditions are 5, 7 and 9 bar and pressure fluctuation frequency conditions are 0, 4, 6 and 8 Hz. When the injection pressure was oscillated by a mechanical pulsator, the spray shape was pulsated regularly. During the pulsated state of the spray with a mechanical pulsator, the spray characteristics, such as spray angle and liquid film thickness in orifice exit, were measured and compared with those in steady state without a mechanical pulsator. Though the mean injection pressure was fixed in the steady and fluctuating state, there were some differences in all measured values, i.e. liquid film thickness and spray cone angle, between both states.

• KSBB Journal
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• 제26권1호
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• pp.69-77
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• 2011

In this study, poly (L-lactic acid) (PLLA) microparticles containing gemcitabine hydrochloride were prepared by a supercritical fluid process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide as antisolvent. The influence of process parameters such as temperature, pressure, $CO_2$ and solution flow rate, solution concentration, and feed ratio of drug to polymer on the morphology and characteristics of the microparticles was studied in detail. The gemcitabine-loaded microparticles exhibited a spherical shape with a smooth surface. The entrapment efficiency of gemcitabine increased with increasing temperature, solution concentration and $CO_2$ flow rate and with decreasing drug/polymer feed ratio. The maximum drug loading obtained from the ASES process was found to be about 11%. The ASES-processed PLLA microparticles containing gemcitabine showed a relatively high initial burst due to the presence of surface pores on the microparticles and the poor affinity between drug and polymer.

• 농업과학연구
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• 제50권2호
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• pp.271-279
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• 2023

The aim of the present study was to investigate the influences of Curcuma aromatica or inositol monophosphate supplementation on body weight of sows at different stages, feed intake, backfat thickness of sows at different stages, body weight of piglets at different stages, and immunoglobulin G (IgG) concentration in sow blood and milk. Eighteen crossbred (Landrace × Yorkshire) sows (249.9 ± 3.2 kg) and their litters were used in a 28-day feeding trial to observe the effects of Curcuma aromatica or inositol monophosphate as dietary supplements on performance and IgG concentration of blood and milk in lactating sows and piglets. The dietary treatments comprised a control corn-soybean-based basal diet (CON); control diet + Curcuma aromatica at 0.5% (CA), and control diet + inositol monophosphate at 0.10% (IMP). Sow body weight at different stages, average daily feed intake, and sow backfat thickness at different stages were not affected in all three treatment groups. The body weight of piglets at weaning and average daily gain of piglets born to sows from the IMP group showed significant improvement compared to piglets of sows from the CA treatment group. Treatment had no effect on the IgG levels in blood and milk. In conclusion, supplementation of 0.5% CA or 0.10% IMP in sows has no effect on growth performance and IgG in sows and piglets compared with the control diet.