• 한국축산시설환경학회지
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• 제13권1호
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• pp.21-28
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• 2007

본 실험은 우리나라에서 사육되고 있는 홀스타인 젖소 착유우의 사료 섭취량과 음수량이 산유량과 분뇨 배설량에 미치는 영향을 구명하기 위하여 수행하였다. 실험은 착유우 10두를 공시하여 수원의 축산연구소 낙농우사에서 봄, 여름, 가을, 겨울 등 4계절에 걸처 실시하였으며 그 결과는 다음과 같다. 1. 시험에 공시된 착유우의 건물섭취량은 평균 19.5kg으로 봄과 가을이 각각 23.9, 22.1kg으로 높았고, 여름과 겨울이 각각 17.0, 15.3 kg으로 낮은 섭취량을 보였다. 2. 착유우의 음수량은 평균 $77.2\ell$로, 여름이 $85.5\ell$로 가장 많았고 겨울이 $62.2\ell$로 가장 낮았으나 계절별 유의차는 보이지 않았다. 3. 착유우의 산유량은 평균 26.0kg으로 봄, 여름, 가을 및 겨울이 각각 30.8, 24.0, 25.4, 23.7kg으로 봄의 산유량이 가장 높았다(p<0.05). 4. 착유우의 분뇨 배설량을 계절별로 조사한 결과, 봄 64.4kg, 여름 63.5kg, 가을 60.4kg, 겨울 51.0kg으로 계절 간에 차이를 보였으며(p<0.05), 전체 평균 분뇨 배설량은 59.8kg이었다. 5. 착유우의 사료섭취량, 음수량, 산유량 및 분뇨 배설량과의 상관관계를 분석한 결과, 산유량과 음수량$(R^2=0.7742)$, 산유량과 사료 섭취량$(R^2=0.7459)$, 음수량과 뇨 배설량 $(R^2=0.7422)$, 사료 섭취량과 분 배설량$(R^2=0.6044)$, 산유량과 분 배설량$(R^2=0.692)$의 상관관계가 비교적 높았으나, 음수량과 분 배설량$(R^2=0.2950)$, 사료 섭취량과 뇨 배설량$(R^2=0.1985)$, 산유량과 뇨 배설량$(R^2=0.2335)$의 상관관계는 낮게 나타났다. 6. 따라서 산유량과 음수량, 산유량과 사료 섭취량의 상관관계식은 $Y=0.1919X_1+11.181(R^2=0.7742),\;Y=0.8568X_2+9.3067(R^2=0.7459)$(Y=milk yield $X_1=water$ consumption, $X_2=feed$ intake)로 추정할 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권4호
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• pp.549-554
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• 2003

In order to estimate the availability of ruminally-protected Lys (RPLys) and Met (RPMet), quantitative changes of free Lys and Met in the total abomasal outflow and feces were determined in three Holstein heifers with a T-shaped duodenal cannula. RPLys was prepared by coating Lys with fat and RPMet with a pH-sensitive polymer. RPLys and RPMet containing 30 g of each amino acid were supplied together to heifers, and total abomasal outflow was collected hourly from the T-shaped duodenal cannula for a 48 h period. Collected abomasal outflows were fractionated into liquid and solid phases to measure free Lys and Met concentration. At 2 wk after total abomasal outflow sampling, heifers were supplied RPLys and RPMet together again, and total feces were collected every 12 h for a 3 d period t estimate excreted RPLys and RPMet in feces. The amounts of Lys and Met recovered from the liquid fractions of abomasal outflow were estimated to be $23.9{\pm}8.3%$ and $68.6{\pm}3.6%$ (p=0.008) of RPLys and RPMet ingested, respectively. Final intestinal disappearances of Lys and Met were estimated to be $49.5{\pm}2.6%$ and $78.2%{\pm}6.5%$ (p=0.015) of ingested RPLys and RPMet, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제28권11호
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• pp.1624-1628
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• 2015

Rigorously standardized laboratory protocols are essential for meaningful comparison of data from multiple sites. Considering that interactions of minerals with organic matrices may vary depending on the material nature, there could be peculiar demands for each material with respect to digestion procedure. Acid digestion procedures were evaluated using different nitric to perchloric acid ratios and one- or two-step digestion to estimate the concentration of calcium, phosphorus, magnesium, and zinc in samples of carcass, bone, excreta, concentrate, forage, and feces. Six procedures were evaluated: ratio of nitric to perchloric acid at 2:1, 3:1, and 4:1 v/v in a one- or two-step digestion. There were no direct or interaction effects (p>0.01) of nitric to perchloric acid ratio or number of digestion steps on magnesium and zinc contents. Calcium and phosphorus contents presented a significant (p<0.01) interaction between sample type and nitric to perchloric acid ratio. Digestion solution of 2:1 v/v provided greater (p<0.01) recovery of calcium and phosphorus from bone samples than 3:1 and 4:1 v/v ratio. Different acid ratios did not affect (p>0.01) calcium or phosphorus contents in carcass, excreta, concentrate, forage, and feces. Number of digestion steps did not affect mineral content (p>0.01). Estimated concentration of calcium, phosphorus, magnesium, and zinc in carcass, excreta, concentrated, forage, and feces samples can be performed using digestion solution of nitric to perchloric acid 4:1 v/v in a one-step digestion. However, samples of bones demand a stronger digestion solution to analyze the mineral contents, which is represented by an increased proportion of perchloric acid, being recommended a digestion solution of nitric to perchloric acid 2:1 v/v in a one-step digestion.

• Asian-Australasian Journal of Animal Sciences
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• 제8권4호
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• pp.403-409
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• 1995

A growth experiment was conducted to evaluate the nutritivie values of supplemental lysine and methionine in broiler chicks. Two types of L-lysine, liquid and powder type, and DL-methionine were added to the diets at different levels of dietary protein with two growth phases, 0-3 weeks and 4-6 weeks named starter and grower, respectively. Six hundred seventy two chicks were allotted in 14 treatments; 3 controls by dietary CP level (starter-grower) with CP 23-21%, CP 21-19% and CP 20-18, 8 groups of liquid and powder lysine supplementation of 0.1, 0.2, 0.3 and 0.4%, and 3 groups of lysine and methionine supplementation. Body weight, feed intake, and excreta were measured and analyzed to determine growth performance, amino acid digestibilities, and the quantity of excreted nitrogen in feces. Chicks fed CP 23-20 with 3,200 ME kcal showed significantly better growth performance than those fed CP 21-18 for 6 weeks. The supplementation of 0.2% of either type of lysine to CP 21-19 diet improved weight gain and feed efficiecy to the extent that CP 23-21 diet was fed. Physical type of lysine did not affect chick's growth and amino acid digestibilities of the diets. The level of CP in the diet significantly affected nitrogen excretion in feces. Supplementation of lysine and methionine to CP 21-18 diet reduced fecal nitrogen by 10% compared to CP 23-21 diet. It was confirmed that 0.2% of supplemental lysine to the broiler diet spared the dietary protein by 3%, and also reduced nitrogen excretion in feces by 10%.

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.614-621
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• 2010

Increasing accuracy of broiler diet formulation based on amino acid digestibility in comparison to application of total amino acids could lead to more feed efficiency and productivity. This experiment was conducted for determination of sampling site (excreta and ileum) and recognition of the effects of a commercial enzyme ($Grind^{(R)}$ Danisco, Finland) on metabolizable energy, protein and amino acid digestibility of barley. This study was modulated by a marker in 21-day old Arbor Acres chickens. Corn-soybean meal was used as a control diet and, in the other two treatments, barley (at a level of 40%) with and without enzyme as the test ingredient were supplemented to the basal diet. Chromic oxide was included in all diets (0.5%) as an indigestible marker. Apparent metabolizable energy (AME), corrected by nitrogen (AMEn) and apparent digestibility of aspartic acid, glutamic acid, serine, glycine, alanine, tyrosine, valine and methionine were significantly (p<0.05) higher in feces than ileum. Protein digestibility of diet and barley was significantly (p<0.05) higher in the ileum than in feces. Apparent digestibility of tryptophan, proline, methionine, phenylalanine and lysine was increased significantly (p<0.05) by enzyme supplementation. In contrast, no response was observed in AME, AMEn, and protein digestibility of the diet and barley by enzyme supplementation. The results of this study have shown that AME and amino acid digestibility were increased in feces, in contrast an adverse effect was observed for protein digestibility of the diet and barley.

• 한국축산시설환경학회지
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• 제7권3호
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• pp.169-172
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• 2001

축산기술연구소 유우사에 있는 계류식 우사에서 홀스타인 착유우 6두를 공시하여 1998년 9월 2일부터 9월 11일까지 10일간 개체별로 계류하여 배합사료를 NRC 사양표준에 기준하여 급여하고 조사료로 옥수수 싸일 레지 및 목건초를 자유채식토록 하여 분뇨배설랑과 특성을 조사하였다. 시험기간중 착유우의 평균체중은 550.0kg 이었으며 평균 사료섭취량은 건물기준 16.7 kg이었다. 산유량은 평균 24.4kg이었으며, 음수량은 85.4l였다. 분뇨배실량은 평균 63.5 kg으로, 분이 42.3, 뇨가 21.2kg이있다. 분뇨의 특성에서 분과 뇨의 수분 함량은 각각 83.9, 96.9%였고, pH는 각각 6.6, 7.6으로 나타났다. 분의 $BOD_5,\;COD_{Mn}$, SS, T-N, T-P는 각각 16,560, 40,329, 78,500, 2,854, 577mg/$\ell$였고, 뇨의 $BOD_5,\;COD_{Mn}$, SS, T-N, T-P는 각각 4.580, 7.575, 370, 4,164, 7mg/l로 나타났다. 분뇨중의 중금속 함량은 상당히 낮은 수준으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권2호
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• pp.279-286
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• 2010

Livestock production in Vietnam is, as in most Asian countries, increasing rapidly and changing into specialized highly intensified operations. The volume of animal excreta generated exceeds the capacity of the operation land base and cannot be utilized efficiently. As a consequence, there is a loss of plant nutrients from livestock farms that causes environmental pollution. This study carried out a feed and excretion experiment measuring fecal characteristic, daily fecal production, daily nitrogen and phosphorous excretion from grower-finisher pigs fed prevalent rations in Vietnam. Furthermore, equations for assessing the excretion were tested, which can be used in farm models for optimal recycling of manure while focusing on reducing pollution. The results indicated that fecal production and nutrient excretion were affected by the different rations tested. This study showed that five selected equations for predicting excretion from grower-finisher pigs in Danish conditions can also be used with precision in Vietnamese pig farming systems. The equations have been proven valid and can, therefore, be used as a much needed tool for assessing fecal production and nitrogen in excreta on pig farms. The study also showed that about 12% of nitrogen excreted was emitted during housing. Waste water contains more than half of the nitrogen excreted, mainly in ammonium form which has a high potential for gaseous emission.

• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.806-809
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• 1999

The present study showed the advantages of dried Bacillus subtilis culture (DBSC) supplementation on reducing ammonia gas release in the poultry house. In Experiment 1, 65-week-old Hyline W-36 hens were raised in individual wire-floor cages in a windowless house, and divided into two groups of 180 hens each. One group was fed diets without DBSC as the control and another group was fed a diet supplemented with 2% DBSC. In Experiment 2, 2-week-old broiler chicks were divided into 3 treatment groups of 20 chicks each and maintained in individual floor cages. One group was fed the diet without DBSC and other two groups were fed the diet supplemented with 1 or 2% DBSC, respectively. In experiment 1, DBSC consistently reduced ammonia gas release in the laying house (p<0.01) and manure storage facilities (p<0.01). incubation of feces for 1, 2, 3, 4, 5, 6, 24 or 48 hours showed that DBSC consistently reduced ammonia gas release. In Experiment 2, DBSC reduced ammonia gas release in the broiler house; however, DBSC had no effect on total N, urate-N and ammonia-N contents of feces, but it improved cumulative N utilization and decreased serum urea-N concentration when chicks when chicks were fed 1% DBSC.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1335-1341
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• 2006

We selected a Lactobacillus spp. from Korean healthy infant feces based upon their antioxidant activity. This strain was identified as Lactobacillus gasseri by 16S rDNA sequencing, and named Lactobacillus gasseri NLRI-312. In the present study, we investigate the protective effect of this strain on the $H_2O_2$ induced damage to cellular membrane lipid and DNA in Jurkat cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA (malondialdehyde) was measured, and DNA damage was tested by the comet assay. We also examined probiotic properties including tolerance to acid and bile, antibiotic resistance. From the results obtained, the supplementation of Jurkat cells with NLRI-312 decreased in DNA damage, while no effect was shown on MDA decrease. In probiotic properties, this strain was resistance to both acid and bile, showed considerably higher survival when incubated in pH 2 or 1% bile salts (w/v). We concluded that the NLRI-312 could be used as potential probiotic bacteria, with the effect of reducing DNA damage induced by $H_2O_2$.

• 대한수의학회지
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• 제36권2호
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• pp.371-378
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• 1996

Three and 2 strains of E coli O157 were isolated from fecal materials of cattle (390) and pigs (420) in Korea, respectively. One strain of O157:H7 isolated from cattle and 2 strains of O157:H7 isolated from pigs were identified as verotoxin-1 (VT-1) produing strains and 2 strains (O157:H7 and O157:H-) isolated from cattle were identified as verotoxin-2 (VT-2) producing strains by neutralization test on HeLa and Vero cells. Culture supernatants of the isolates were cytotoxic to HeLa and Vero cells. The levels of cytotoxin produced by isolates were $10^2{\sim}10^4$ cytotoxic dose($CD_{50}$)/ml. Also, VT-2-converting bacteriophage was isolated from KSC109 strain which had been isolated from cattle. Molecular weight of the phage DNA was determined as approximately 45 Kb in 0.8% agarose gel electrophoresis, and morphology of the phage stained with phosphotungstic acid was observed by transmissible electron microscopy.