• 경영정보학연구
• /
• 제10권2호
• /
• pp.195-209
• /
• 2008

고객의 니즈가 시시각각 변화하는 경영환경에서 획일화된 매장관리 방법으로 매장의 수익성을 증대시키기에는 한계가 있다. 따라서 고객의 선호 변화를 예측하여 각 매장에 적절한 상품을 추천할 필요가 있다. 본 연구에서는 판매 데이터 분석을 통해 시간 순서를 고려한 상품 추천 및 매장관리 방법을 제안한다. 즉 자기조직화지도(Self Organizing Map) 알고리즘을 이용하여 매장의 판매 프로파일을 군집화하고, 매장 궤적의 예측을 통해 목표 매장을 관리하는 방법을 제시한다. 본 연구의 방법론을 검증하기 위해 (주)더페이스샵 판매데이터를 적용하여 평가하였으며, 평가결과 제시한 방법론은 화장품처럼 유행에 민감하고 라이프사이클이 짧은 특징을 지닌 상품을 판매하는 매장의 수익성 증대에 기여할 수 있을 것으로 기대된다.

• Journal of Microbiology and Biotechnology
• /
• 제8권6호
• /
• pp.656-662
• /
• 1998

To make a distinction between the influence of the dielectric constant and of methanol concentration on trypsin-catalyzed hydrolysis and methanolysis at $0^{\circ}C$, a model reaction of $N^u$-benzyloxycarbonyl-L-lysine p-nitrophenyl ester with water-methanol mixtures was chosen and a kinetic study done. The $k_{cat}$ values increased with methanol concentration, in a linear manner whereas $K_{M}$ values increased in a log-linear fashion. However, the $k_{cat},$_{M}$ ratio increased at lower methanol concentrations than 30% and then began to decrease at higher concentrations. The decrease in $k_{catK_M}$observed at higher than 30% methanol concentrations is attributed to the hydrophobic partitioning effect on substrate binding. On the other hand, the increase in $k_{catK_M}$ in the 0~30% methanol concentration range seems to be due to the effect of nucleophilic cosolvent on $k_{cat}$ and of the dielectric constant on $k_m$. This explanation was verified by measuring the effect of varying the dielectric constant of the medium on kinetic constants with isopropyl alcohol chemically unrelated to the enzyme reaction as the methanol concentration is maintained at a constant level. Therefore, we conclude that the effect of increasing the methanol concentration in the model reaction on the kinetic parameters $k_{cat \;and\;{K_M}}$ is caused by changes in both the nucleophilicity and the dielectric constant of the medium. Based on product analysis, the increase in $k_4, k_3$by decreasing the temperature can be accounted for by the suppression of hydrolytic reactions. This observation indicates that the nucleophile is favored by low temperatures. There was no loss of trypsin activity over a 10 h period in 60% methanol concentration at $pH^＊\; 5.5,\; 0^{\circ}C$.EX>.

• 한국실내디자인학회논문집
• /
• 제21권2호
• /
• pp.123-132
• /
• 2012

In the trend of concentrating on the consumer driven experience market as a new marketing concept according to the improvement of life standard and the change of consummation pattern, SPA brand has applied experience marketing strategies to the market successfully to be grown constantly with consumer secure and production of business benefit through brand image and positive consumer attraction. Therefore, this study aims to research the experiential representation element and attribute in the competitive Global SPA brand space due to the sustainable growth from the recent domestic fashion market based on the strategy type of the experiential marketing. Thus, the experiential marketing strategy type was drawn based on the experiential marketing and the theoretical reflections of Global SPA brand, and the SPA brand space was classified depending on the attribute of the commercial space for making the framework of case analysis, so it was progressed as the method of analysis through the experiential representation attribute in the SPA brand space. The marketing strategy and representation for advertising the image of company and product message by the SPA brand should be planned, so successful application of the experiential marketing to the shop is connected to the corporate interests, and forming the meaning more than space by impressing on the consumers the brand and arousing the emotional experience of the consumers and meeting the consumers' a variety of needs had effects on forming a lasting relationship between the brand and consumers. Therefore, this study is expected to be an opportunity to vitalize the domestic SPA brand behind the competition with the Global SPA brand.

• Journal of Microbiology and Biotechnology
• /
• 제1권3호
• /
• pp.188-196
• /
• 1991

The ${\beta}-glucosidase$ was purified to homogeneity from the culture filtrate of P. verruculosum by column chromatography. The enzyme was a glycoprotein with a relative size of approximately 220 kDa with an isoelectric point of 4.8, which was composed of dimeric protein of 105 kDa. The enzyme was stable up to $60^{\circ}C$ and the presence of glycerol significantly increased its thermostability. The enzyme was found to hydrolyze both ${\beta}-aryl$ and ${\beta}-alkyl-glucosides$ in addition to ${\beta}-glucosyl$ glucose and catalyzed glucosyl transfer to cellobiose. The enzyme attacked laminarin in an exotype-like fashion. The apparent Km's of the enzyme toward cellobiose, laminaribiose, laminarin were 0.53 mM, 0.35 mM and 1.11 mM, respectively. Glucose and glucono-${\delta}-lactone$ were competitive inhibitors for the enzyme. Copper ($Cu^{2+}$), mercury ($Hg^{2+}$) and p-chloromercuribenzoate were strong inhibitors of the enzyme. The immunoblotting result revealed that one form of ${\beta}-glucosidase$ was biosynthesized, irrespective of carbon sources used. Polyacrylamide gel electrophoresis analysis of the in vitro translated product of total RNA from avicel grown mycelium established that the P. verruculosum ${\beta}-glucosidase$ precursor was approximately 95 kDa in size. The amino acid composition and N-terminal amino acid sequence are given.

• 복식문화연구
• /
• 제10권3호
• /
• pp.236-247
• /
• 2002

In spite of the bad conditions of fashion companies: the number of licit wear consumers in Korea are increasing because knit wear gives a body with an comfort action. Domestic knit wear industry, however, doesn't have any standard fur satisfying consumers'needs and for quality estimation on that. So we examined the knit wearers'conception of the quality to broaden the knowledge of knit wear and produce better products. The purpose of this study was to find out the factors of wearer's perception. This study carried out through a qualitative approach. The subject of this study were 17 single women who were 20's and living in Seoul and Kyoungki province. The item of this questionary was as follow: 1) what they know about knit wear and how much they are satisfied with that 2) what the wearers think the problems of knit wear are 3) how knitted wear industry can satisfy consumers's needs and take into account consumer feedback in product development The results of this study were summarized as follows. 1. The wearers had no knowledge about materials, sewing methods, patterns, knitting methods, and gage. 2 The wearers didn't recognize the differences between the products by famous brand and obscure conventional market. For that reason, the high price knit wear wont satisfy wearer. 3. The wearers didn't prefer the revealing their body shapes. 4. Laundering was the big problem which was they chosen the knit wear. They take it for granted that the knit wear needs dry-cleaning or hand-washing. Knit wear industry tries to make it easy laundering by development knit material.

• 복식문화연구
• /
• 제16권1호
• /
• pp.33-47
• /
• 2008

The purposes of this study were to extract the effects of emotional labor, self-efficacy and burnout on customer-oriented selling behavior of salesperson by literature review and to examine the effect variables on customer-oriented selling behavior empirically. The questionnaire was administered to 400 salespersons who were selling clothes, cosmetics, shoes, leather products and accessories in department stores located in Kwang-ju city during August 18-24, 2006. Data collected from 307 salespersons were analyzed by using descriptive statistics, factor analysis, t-test, ANOVA, Duncan-test, correlation, regression analysis and path analysis. The results were as follows: First, Salesperson's emotional labor was composed of two factors; emotional labor toward the customer and emotional labor toward the organization. Second, Salesperson's emotional labor had negative significant influence on customer-oriented selling behavior. Specifically, the emotional labor toward the organization negatively influenced on customer-oriented selling behavior, but the emotional labor toward the customer positively influenced on customer-oriented selling behavior. Third, Salesperson's emotional labor positively influenced on burnout. Specifically, emotional labor toward organization significantly influenced on burnout, but emotional labor toward customer did not influenced on burnout. Fourth, Salesperson's self-efficacy positively influenced on customer-oriented selling behavior. Fifth. Salesperson's self-efficacy negatively influenced on burnout. Finally, Salesperson's burnout negatively influenced on comer-oriented selling behavior. The direct effect of salesperson's self-efficacy on customer-oriented selling behavior was large than the indirect effect of salesperson's self-efficacy mediate burnout. But the indirect effect of salesperson's emotional labor mediate burnout was large than the direct effect of salesperson's emotional labor on customer- oriented selling behavior.

• BMB Reports
• /
• 제38권5호
• /
• pp.584-590
• /
• 2005

Glucose 6-phosphate dehydrogenase (EC 1.1.1.49) was purified from Aspergillus aculeatus, a filamentous fungus previously isolated from infected tongue of a patient. The enzyme, apparently homogeneous, had a specific activity of $220\;units\;mg^{-1}$/, a molecular weight of $105,000{\pm}5,000$ Dal by gel filtration and subunit size of $52,000{\pm}1,100$ Dal by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The substrate specificity was extremely strict, with glucose 6-phosphate (G6P) being oxidized by nicotinamide adenine dinucleotide phosphate (NADP) only. At assay pH of 7.5, the enzyme had $K_m$ values of $6\;{\mu}m$ and $75\;{\mu}m$ for NADP and G6P respectively. The $k_{cat}$ was $83\;s^{-1}$. Steady-state kinetics at pH 7.5 produced converging linear Lineweaver-Burk plots as expected for ternary-complex mechanism. The patterns of product and dead-end inhibition suggested that the enzyme can bind NADP and G6P separately to form a binary complex, indicating a random-order mechanism. The enzyme was irreversibly inactivated by heat in a linear fashion, with G6P providing a degree of protection. Phosphoenolpyruvate (PEP), adenosinetriphosphate (ATP), and fructose 6-phosphate (F6P), in decreasing order, are effective inhibitors. Zinc and Cobalt ions were effective inhibitors although cobalt ion was more potent; the two divalent metals were competitive inhibitors with respect to G6P, with $K_i$ values of $6.6\;{\mu}m$ and $4.7\;{\mu}m$ respectively. It is proposed that inhibition by divalent metal ions, at low NADPH /NADP ratio, is another means of controlling pentosephosphate pathway.

• 한국안광학회지
• /
• 제15권2호
• /
• pp.131-136
• /
• 2010

목적: 민화의 한국적 이미지를 활용한 안경 소품디자인의 연구개발. 방법: 전통민화에 나타나는 생동감 있는 색채와 독특한 조형미를 분석하고 그러한 미의식을 안경클리너와 안경케이스에 적용하여 현대적 미감을 가진 디자인으로 재해석한다. 특히 안경클리너와 안경케이스의 넓은 평면은 시각적으로 한국적 특징을 표현하기에 적합한 공간을 가지고 있다. 결과: 현재 민화는 세계적으로 주목받는 문화콘텐츠이다. 그러므로 민화를 활용하여 제품의 이미지를 문화적 이미지로 변화시킴으로써 '문화적 소비'를 촉진할 수 있다. 결론: 민화의 예술성을 접목한 안경소품 디자인은 안경 제품의 이미지에 긍정적인 영향을 줌으로써 세계시장에 안경소품디자인 시장이 확대될 것을 기원한다.

• Natural Product Sciences
• /
• 제19권4호
• /
• pp.290-296
• /
• 2013

In preliminary tests, we examined the effect of several fractions isolated from fermented pine needle extract on pacemaker potentials in cultured interstitial cells of Cajal (ICCs) from the mouse colon using a whole cell patch clamp technique. Among these fractions, Fraction 3 (F3) elicited the most powerful depolarization of membrane. Therefore, the aim of the present study was to investigate the effect of F3 obtained from fermented extract of Pinus densiflora needle on pacemaker potentials in ICCs and to establish its mechanism of action. Colonic ICCs generated spontaneous periodic pacemaker potentials in the current-clamp mode. F3 depolarized the membrane and decreased the frequency and amplitude of pacemaker potentials in a dose-dependent fashion. The F3-induced effects on pacemaker potentials were blocked by methoctramine, a muscarinic $M_2$ receptor antagonist, and by glycopyrrolate, a muscarinic $M_3$ receptor antagonist. The F3-induced effects on pacemaker potentials were blocked by external $Na^+$-free solution and by flufenamic acid, a non-selective cation channel blocker, as well as by the removal of external $Ca^{2+}$ and in the presence of thapsigargin, a $Ca^{2+}$-ATPase inhibitor in the endoplasmic reticulum. Taken together, these results suggest that F3 of pine needle extract modulates the pacemaker activity of colonic ICCs by the activation of non-selective cation channels via muscarinic $M_2$ and $M_3$ receptors. And external $Ca^{2+}$ influx and intracellular $Ca^{2+}$ release are involved in F3 actions on ICCs.

• 복식문화연구
• /
• 제26권5호
• /
• pp.649-664
• /
• 2018

With the rapid growth of the Chinese cosmetics market and the continuous introduction of new products into the market, beauty videos generated by other consumers, that are by beauty vloggers, have become a major source of information for cosmetics consumers. With the popularity of beauty vlogs in China, beauty vloggers have become influencers who affect consumers' information processing and attitude development regarding cosmetic products and beauty routines. The current study aims to explore the characteristics of beauty vloggers that affect consumers' attitudes toward both the beauty vlogs and the products featured in those vlogs. Based on parasocial interaction theory, the study examines whether perceived characteristics of beauty vloggers influence consumers' perceived parasocial interaction, which in turn influences their attitudes. The study employs an online survey, which was administered to female consumers in China. Data from a total of 372 responses was used for analysis. Findings show that the perceived similarities to and perceived trustworthiness of vloggers have a significant impact on parasocial interactions, which influences consumer attitudes toward both the vlogs and the products shown in them. Perceived reliability and perceived attractiveness are shown to have a positive impact on consumer attitudes toward vlogs. Perceived expertise, attractiveness, similarity, and trustworthiness have all been shown to affect product attitudes. These results show that while beauty vlogs have a significant impact on consumers' decision-making processes, the perceived characteristics of beauty vloggers themselves are important to consumers in the development of their attitudes toward both vlogs and products.