• The Plant Pathology Journal
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• v.22 no.1
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• pp.21-27
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• 2006

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.42 no.2
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• pp.78-85
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• 2006

A survey on the fishing gear for octopus drift-line fishing was done in Gangwon-do, Sacheon, Gyeongnam and Hokkaido, Japan, while a survey on the environments of fishing grounds used data from January to August from the Korea east coast farming forecast system of Donghae Fisheries Research and Development Institute. The present situation of fishing was examined with boats engaging in drift-line fishing from March to August in the coastal waters along Gangneung. The fishing tackle for octopus drift-line fishing was made manually, and the size, shape, and weight of the hook and number of shooting used vary according to the fishing time and region. Lead is used as the material for sinkers. As bait, pork fat with skin is mostly used in Gangwon-do. The temperature of the bottom water layer in the coastal fishing ground of Gangneung from April to June ranges from $3.2-12.4^{\circ}C$, which is the optimal temperature for octopuses. During July and August, the temperature ranges from $5.0-20.6^{\circ}C$. The maximum difference between day and night temperatures reached up to $9.2^{\circ}C$. Salinity is generally stable at $33.2-35.324.6%_{\circ}$, which does not affect the inhabitation of octopuses. The octopus catch reached its peak from May to July, while most octopuses weighed less than 1 kg (76.7%). The results show that it is effective to carry out octopus drift-line fishing up to a depth of 40 meters; and that the maximum number of octopuses per line is obtained with an operation time of six hours.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.886-891
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• 2013

We analyzed surface sediments to explain the substrate characteristics of sea cucumber Stichopus japonicus habitat. The analyses included grain-size determination, water content (WC), loss on ignition (IL), chemical oxygen demand (COD), and acid volatile sulfide (AVS) content. We obtained data on sea cucumber density, weight, and length at each station in a marine farming area in the middle of Korea's Taean coast on the West (Yellow) Sea in May, 2012. The apparent density of S. japonicus was high in coarse and bimodal sediments, whereas it was low in fine and unimodal sediments. The mass of S. japonicus was greater in fine than in coarse sediments, showing a different trend from sea cucumber density. The values for WC, IL, COD, and AVS in surface sediments were higher in areas with high densities of S. japonicus than in areas with low sea cucumber densities. In particular, the concentration of AVS was much higher than the value proposed in Japanese criteria for areas with high S. japonicus density.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.12
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• pp.400-406
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• 2016

Comparing the past and the present in agriculture, society is losing people who work in farming, and the age of those who remain is increasing. Farmers are interested in special crops if the agricultural products' costs are low and the crops are easy to grow. If the area where a crop grows is bad, the agricultural products' quality gets worse. To overcome this situation, a new approach is being tried with crops. This research offers new technology to the young generation. This paper proposes technology that uses Internet of Things techniques to automatically sparge water and pesticide on orchards and fields using a machine instead of a person. We used the open source Arduino and sensor modules to build the automatic system. In this research, a circuit was simplified, and we constructed the proper size of the system by preventing errors in sensors, keeping distance from objects, and minimizing circuit collision. The machine drives and turns its head to sparge agricultural pesticides. The machine will minimize harmful effects caused by pesticides on humans, and will be helpful to farmers.

• Journal of Agricultural Extension & Community Development
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• v.19 no.1
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• pp.1-28
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• 2012

The Extension Program accountability will continue to be an influential force to develop agriculture and rural area. As we incorporate new approaches into our service program and respond to new domains of knowledge, we must also consider how our assessment strategies may need to change in order to meet local and other external standards. The main purpose of the study was to be what the best way of Extension Service is set farmers' educational needs met and to be what kind of methodologies and role of Extension system should provide for their faced problem solving effectively. The major results of the study on Farmers' needs toward Korean Extension Services are summarized as follows. First, it is observed that, when the farmers' perceptions were analyzed, the most ranked by the farmers' needs was "having nice extension workers to get advice and solve their problems on the farm in all at once." Farmers had especially more positive attitudes in the categories of interest and careers in ag-technology. To do services level up, the headquarter of Rural Development of Administration must be work with local branches to effectively deliver their missions and educational objectives to target crops and farmers group. And farmers were strongly needed "a specialized service team will be organized" to work closely with operating services for ag-products processing, packing and marketing. The recommendations were made by: farmers' needs should be identified and announced by computer networking development to solve their problems faster. Also, provincial and county level service members should have a chance to working at research institute or related work places with their incentives, which system will be easer to meet farmers' needs and deal with their problems so that the systematic approaches should be managed and recorded their educational attendings, education contents and even their farming corporations. Finally, Agricultural Extension Service should be met to general ag-technology literacy and be balanced with knowledge of other industries and be competitive service with better values in non-agricultural sectors.

• Journal of Korean Society of Rural Planning
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• v.21 no.4
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• pp.187-196
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• 2015

Among the factors of living environment, the one that is the most closely related with our living is the building. It is one of the biggest reasons for energy consumption as it forms 36% of the total energy consumption. Technologies equipped with excellent energy performance can hardly be applied to rural areas that are relatively poorer. Still, 89.8% rural residents are living in detached houses, and backward houses increase their financial burden and result in reduced insulation performance. Accordingly, this study is going to review the latest research written after 2000 dealing with rural houses and their insulation. The purpose of this study is to analyze the factors of insulation and how to improve insulation performance, conduct field research to find out how to apply low energy technologies applicable to houses with the subjects of experimental houses, the passive houses located in Jecheon City, Hongcheon Saldun zero energy houses, and energy independence villages, and find out how to perform follow-up research on insulation for rural houses. According to the findings, the latest research on insulation for rural houses is mostly focused on walls as well as windows and doors. Also, as ways to improve insulation performance, it suggests us to use high performance insulators, introduce new regeneration energy technology, and secure hermeticity. In addition, through field research, this author could find out low energy technologies applicable to houses such as solar energy facilities and heat recovery systems. Advanced research on insulation for rural houses has been focused on how to use materials or new regeneration energy, so follow-up research will have to consider the types of farming area or the residents' mode of living.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.4
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• pp.407-418
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• 2020

Mass mortality of mariculture fish due to high summer temperatures is a major issue in the mariculture industry in many coastal waters of Korea, yet measures to mitigate the impact are generally limited. We injected a micro-bubble of liquefied oxygen into the bottom of rockfish cages (about 6-8 m deep) in order to maximize the dispersal of micro-bubbled seawater and reduce fish mortality. The injection of low-temperature oxygen in micro-bubbles lowered the water temperature at the injection area by as much as 1℃ and increased dissolved oxygen concentration by 0.5 ppm. In early August, following a week with persistent high water temperature (above 28.5℃), there was an increase in fish mortality despite the micro-bubble system, which resulted in approximately 7% death of the total introduced fish population. However, this mortality appeared to be much lower than mortality reported in a neighboring mariculture facility (approximately 50% mortality). We also estimated the volume that can be recirculated with pumped seawater using a micro-bubble system. We suggest that this approach of injecting liquefied oxygen through a micro-bubble system may reduce fish mortality during high temperature periods.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.603-621
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• 2017

The current review focuses on characterization and conservation efforts vital for the development of breeding programmes for indigenous beef cattle genetic resources in Southern Africa. Indigenous African cattle breeds were identified and characterized using information from refereed journals, conference papers and research reports. Results of this current review reviewed that smallholder beef cattle production in Southern Africa is extensive and dominated by indigenous beef cattle strains adaptable to the local environment. The breeds include Nguni, Mashona, Tuli, Malawi Zebu, Bovino de Tete, Angoni, Landim, Barotse, Twsana and Ankole. These breeds have important functions ranging from provision of food and income to socio-economic, cultural and ecological roles. They also have adaptive traits ranging from drought tolerant, resistance to ticks and tick borne diseases, heat tolerance and resistance to trypanosomosis. Stakeholders in the conservation of beef cattle were also identified and they included farmers, national government, research institutes and universities as well as breeding companies and societies in Southern Africa. Research efforts made to evaluate threats and opportunities of indigenous beef cattle production systems, assess the contribution of indigenous cattle to household food security and income, genetically and phenotypically characterize and conserve indigenous breeds, and develop breeding programs for smallholder beef production are highlighted. Although smallholder beef cattle production in the smallholder farming systems contributes substantially to household food security and income, their productivity is hindered by several constraints that include high prevalence of diseases and parasites, limited feed availability and poor marketing. The majority of the African cattle populations remain largely uncharacterized although most of the indigenous cattle breeds have been identified.

• Journal of Animal Science and Technology
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• v.53 no.6
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• pp.549-561
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• 2011

Livestock wastewater is being discharged without treatment from Hasen's pig farm cluster in WangGoong (WG) area into the Iksan Stream, eventually flowing into the ManGyung (MG) at the upstream junction. Although it is well known that before discharge, wastewater must satisfy the pig slurry discharge standards; because of ongoing remodeling, proper treatment is not being performed. According to public records, wastewater from the WG pig farm cluster is responsible for 3.6% of MG River pollution and 2.0% of the SaeManGuem (SMG) Reservoir pollution. As a result, upstream water treatment quality has become primary concern for development of the SMG project. All physicochemical constituents and pathogenic microbes, such as chemical oxygen demand ($COD_{Cr}$), biochemical oxygen demand ($BOD_5$), total suspended solids (TSS), total nitrogen (TN), total phosphorous (TP), fecal coliforms, Escherichia coli and Salmonella at the effluent of WG Plant (S-1) exceed the effluent standards. This is mainly due to insufficient wastewater treatment: the WG Plant is under renovation to increase water purification efficiency. By comparing the water quality at the S-7 junction, where the the Iksan Stream (pig farms) and the Wanggoong Stream (no pig farms) merge, it is clear that farming facilities and improper treatment can critically affect surrounding water quality. While it is clear throughout this study that the level of all physicochemical parameters and pathogenic microbes along the Stream decreased due to sedimentation, biodegradation and/or dilution. An alarming problem was discovered: the existence of pathogenic microbe count(E coli, Salmonella) in the lagoon wastewater and the stream water. Not only were high concentrations of these pathogens themselves found, but the potential existence of more serious pathogens could rise to more dangerous conditions.

• Journal of Animal Science and Technology
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• v.56 no.1
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• pp.2.1-2.8
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• 2014

Hanwoo (Korean cattle) is the native, taurine type of cattle breed of Korea and its history as a draft animal dates back to 5000 Years. In earlier times Hanwoo was used extensively for farming, transportation. Over the period of time, Hanwoo has changed to be meat type cattle. Full-scale production of Hanwoo as meat-type cattle has occurred since 1960s with the rapid growth of the Korean economy. Hanwoo is one of the most economically important species in Korea as it is a significant source of nutrition to the Korean people. Hanwoo beef is the most cherished food of Korea. One of the main goals of researchers is to increase the meat quality, quantity and taste of the beef. In this review we describe the origin, domestication of Hanwoo cattle and breeding program initiated from 1980's. Moreover the advent of technological advancement had provided us a platform to perform genome wide selection on economic traits and its implementation into traditional breeding programs.