• Journal of Microbiology and Biotechnology
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• 제30권6호
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• pp.937-945
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• 2020

N-acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) plays a major role in development of biofilms, which contribute to rise in infections and biofouling in water-related industries. Interference in QS, called quorum quenching (QQ), has recieved a lot of attention in recent years. Rhodococcus spp. are known to have prominent quorum quenching activity and in previous reports it was suggested that this genus possesses multiple QQ enzymes, but only one gene, qsdA, which encodes an AHL-lactonase belonging to phosphotriesterase family, has been identified. Therefore, we conducted a whole genome sequencing and analysis of Rhodococcus sp. BH4 isolated from a wastewater treatment plant. The sequencing revealed another gene encoding a QQ enzyme (named jydB) that exhibited a high AHL degrading activity. This QQ enzyme had a 46% amino acid sequence similarity with the AHL-lactonase (AidH) of Ochrobactrum sp. T63. HPLC analysis and AHL restoration experiments by acidification revealed that the jydB gene encodes an AHL-lactonase which shares the known characteristics of the α/β hydrolase family. Purified recombinant JydB demonstrated a high hydrolytic activity against various AHLs. Kinetic analysis of JydB revealed a high catalytic efficiency (k_cat/K_M) against C4-HSL and 3-oxo-C6 HSL, ranging from 1.88 x 10⁶ to 1.45 x 10⁶ M^-1 s^-1, with distinctly low K_M values (0.16-0.24 mM). This study affirms that the AHL degrading activity and biofilm inhibition ability of Rhodococcus sp. BH4 may be due to the presence of multiple quorum quenching enzymes, including two types of AHL-lactonases, in addition to AHL-acylase and oxidoreductase, for which the genes have yet to be described.

• 인터넷정보학회논문지
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• 제3권4호
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• pp.83-94
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• 2002

최근 소프트웨어 개발의 생산성 향상을 위해 보다 큰 단위의 재사용을 가능하게 하는 컴포넌트 기반 소프트웨어 생산라인에 대한 연구가 활발히 진행되고 있다. 이는, 소프트웨어 생산라인의 자산에 존재하는 일반적인 컴포넌트들을 구체화하고 조립함으로써 고품질의 특정 응용 프로그램을 빠르게 개발하는데 그 목적이 있다. 컴포넌트 기반 소프트웨어 생산라인 구축의 핵심을 컴포넌트의 품질이며. 컴포넌트의 품질을 결정짓는 가장 중요한 특성 중의 하나가 재사용시 재사용자가 컴포넌트의 기능과 구조를 목적과 환경에 맞게 변경학 수 있도록 해주는 '재구성성(reconfigurability)'이다. 본 논문에서는, 특성 다이어그램과 XML/XSLT 기술을 이용하여 컴포넌트 코드 생성 시에 재구성 성을 지원하는 컴포넌트 코드 자동 생성 기법을 제안한다. 본 논문의 기법은, 컴포넌트 패밀리의 특성 다이어그램에 표현되어 있는 차이점들에 대하여 특정 값이나 선택을 제공함으로써 컴포넌트 재사용자가 자신의 목적에 맞는 구체적인 컴포넌트의 소스 코드를 자동으로 생성할 수 있게 해 준다. 사례 연구로서 리스트 컨테이너 패밀리에 대한 컴포넌트 코드 시스템을 개발함으로써 특성 모델과 XML 관련 기술의 자동 생성 프로그래밍 영역에서의 적용 가능성을 보여준다. 본 논문의 코드 생성 시스템은 컴포넌트 기반 소프트웨어 생산라인 구축의 기반 기술로서 이용될 수 있으며, 보다 큰 단위의 컴포넌트 생성을 위한 기반으로 확장 가능하다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2547-2554
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• 2014

Background: To evaluate the perception of cancer patients toward treatment services and influencing factors and to inquire about the use of complementary alternative medicine (CAM). Materials and Methods: Information was obtained through pre-tested structured questionnaires completed by cancer patients during treatment at King Abdulaziz University Hospital, Jeddah, Saudi Arabia. Results: Of 242 patients, 137 (64.6%) accepted to enter this study. Most were Saudi (n=93, 68%), female (n=80, 58%), educated at university (n=71, 52%), married (n=97, 72%) and with breast cancer (n=36, 26%). One-hundred (73%) patients were satisfied with the services provided; 61% were Saudi. Ninety-four (68%) respondents were satisfied with the explanation of their cancer. Twenty-eight (21.6%) patients received CAM, of them 54.0% received herbal followed by rakia (21.0%), nutritional supplements/vitamins (7.0%) and Zamam water (18.0%), with significant differences among them (p =0.004). Seven (5%) patients believed this therapy could be used alone; 34 (25%) patients believed it could be used with other treatments, regardless of whether they themselves used this therapy. Fifty-three (53%) satisfied patients felt they received enough support; 31 (58%) patients received support from family and friends; 22 (41.6%) patients received support from the health-care team. Patients who received information about their disease from their physicians and those who felt they had enough support were more satisfied. The patients who took alternative treatment were older age, mostly female and highly educated but values did not reach significance. Conclusions: We stress enhancing the educational and supportive aspects of cancer-patient services to improve their treatment satisfaction and emphasize the need for increasing the educational and awareness programs offered to these patients.

• Journal of Nutrition and Health
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• 제14권4호
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• pp.190-199
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• 1981

A survey of nutrition and incidence of parasites was conducted from January 23 to 31 and February 14 to 18, 1981, to determine the nutritional status of young children under the age of 6 years, in one of the rural family health project areas: Whaseoung-gun, Kyunggi-do, Korea. This study covered a total of 233 children. The results are summarised as follows: Approximately one-half of these families had an annual income between \1,000,000 and \2,500,000. Nearly 84% of the households were involved in farming. Energy intake of children met 70.6% of the requirement: carhohydrates provided 77%, protein provided 11%, and fat provided 12% of the total energy intake. Low intake of iron; calcium; and vitamins, excluding thiamin, was also found. Mean hemoglobin value was $10.6{\pm}1.0gm/100ml.;\;42%$ of subjects were categorized as low group, 22% were in the deficient group, and only 36% were at an acceptable level as indicated by O'Neal. Thus, 22% of the subjects were proven to be anemic by the hemoglobin criteria. Mean hematocrit value was $30.1{\pm}2.8%$, and 80 of 208 children, or 38.4% of subjects, were below 30% of the standard established by O'Neal. The positive prevalence of parasitic infection was 20% , significantly higher than that observed in the Yowido compound (5.5%) in 1980. Hematologic findings had no significant correlation to parasitic infection. The majority, 95.2% of the subjects, met or surpassed 90% of the KlST's standard of height:whtile 28. 6% of the subjects were below 90% of the KIST's standard of weight. The ratio of weight to height for boys 4 to 6 years old was 15.8 to 17.5, in contrast to 16.3 to 16.9 for boys of KHDI project areas, and also in contrast to 17.7 to 17.9 for boys of the same age group from the Yowido apartment compound in Seoul. According to the results of the assessments, the authors found a clear difference in growth values, hematologic findings and dietary patterns between rural areas and advantaged urban areas of Seoul. Our results suggest the need to develop the nutrition and parasite control training of village women for better nutritional care of their young children.

• Child Health Nursing Research
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• 제9권3호
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• pp.309-322
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• 2003

Purpose: This study was carried out to show the parental child-rearing attitudes perceived by daughters(woman college students), who were in the process of pursuit of resonable values, ethics and preparing for marriage and settling down, and another purposes were to help them to have positive attitudes toward their child-rearing in the future and set up preliminary parenting education program. Method: The subjects were 120 woman college students who were freshmen to junior in one university in Seoul. Using the self-report questionnaire, data were collected from December in 1999 to March in 2000. The contents which subjects wrote down were categorized by content analysis method. Result: 1) The age range of subjects were 19-25 years old and main rearer was mother(85.84％). 2) The perception of parent's child-rearing by subjects were revealed 555 statements and then tied together 50 themes: 'emphasizing on studying hard'(8.3％), 'doing by oneself'(5.6％), 'sternness'(5.2％), 'respect of personality'(4.5％), 'eagerness'(4.0％), 'humanity education'(4.0％), 'moderating in daily living'(4.0％), 'propriety education'(3.8％), 'sacrificing for children'(3.6％), 'expectation'(3.2％), 'concerning'(3.1％), 'parent-centered'(3.1％), 'giving a free hand'(2.9％), 'sharing with'(2.9％), 'consideration'(2.7％), 'over-protectiveness'(2.3％), 'hostile attitude' (2.2％), 'corporal punishment'(2.2％), 'expression of parental love'(2.0％), 'encouragement'(1.8％), 'family-centered' (1.8％), 'treating fairly'(1.6％), 'offering live experiences'(1.6％), 'exacting obedience'(1.62％), 'exemplary parental role' (1.6％), 'sexual discrimination'(1.6％), 'cooperation'(1.4％), 'giving favors'(1.4％), 'indifference'(1.4％), 'understanding' (1.3％), 'confidence'(1.3％), 'intimacy'(1.3％), 'pursuit of health'(1.3％), 'warm-hearted'(1.1％), 'stinginess'(1.1％),'broad-mindedness'(0.7％), 'granting'(0.7％), 'interfering'(0.7％), 'reproach'(0.7％), 'distinction of sex in household affairs'(0.7％), 'help'(0.5％), 'preparing for the future'(0.5％), 'disregarding'(0.5％), 'making environment'(0.4％), 'bringing up a child personally' (0.4％), 'comparing'(0.4％), 'religious life'(0.4％), 'good deed'(0.4％), invasion of privacy' (0.2％), 'controlling desire'(0.2％). 3) 50 themes were categorized by 18 categories once more: 'control' (13.2％), 'crazy for education'(12.3％), 'endeavoring'(8.6％), 'autonomy'(8.5％), 'home education'(7.7％), 'esteem' (6.67％), 'support'(6.67％), 'pursuit of healthy daily life'(5.6％), 'earnestness'(5.4％), 'disregarding personality'(5.4％), 'emotional bonding'(3.2％), 'imposing a burden'(3.2％). 'inhospitality'(3.1％), 'acceptance'(5％), 'discrimination'(2.3％), 'mature parenthood'(1.8％), 'strengthening family tie'(1.8％), 'psychological intimacy'(1.1％). 4) On the basis of this study, 3/5 of subjects(61.2％) perceived their parents had mature and autonomous child-rearing attitudes, and on the other hand 2/5(39.5％) of them perceived controlling, just forcing to work harder and personally disregarding attitudes. Conclusion: So we need to offer them nursing implementation such as preliminary parenting education program and parenting consulting like to strengthen positive perception and help improving in realistic, developmental child-rearing attitudes.

• Tuberculosis and Respiratory Diseases
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• 제71권2호
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• pp.106-113
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• 2011

Background: While asthma control is defined as the extent to which the various manifestations of asthma are reduced by treatment, current guidelines of asthma recommend assessment of asthma control without consideration of airway inflammation. Our aim was to investigate the relationships between fractional exhaled nitric oxide (FeNO), a reliable marker of airway inflammation, and levels of asthma control in patients treated with inhaled corticosteroids (ICS). Methods: We enrolled 71 adult patients with asthma who had been treated with ICS for more than four months. FeNO was measured and spirometry was performed at the time of enrollment. Asthma control was assessed (a) by the physician based on the Global Initiative for Asthma guidelines, (b) by the patients, and (c) by using the Asthma Control Test (ACT). Statistical analyses were done to analyze the relationships between (i) FeNO and (ii) measures of asthma control and clinical indices for asthma manifestations. Results: There was no significant difference in FeNO levels between the three groups according to levels of asthma control (controlled, partly controlled and uncontrolled) as determined by the physician (p=0.81), or by the patients (p=0.81). In addition, FeNO values were not significantly correlated with the ACT scores (r=0.031, p=0.807), while FeNO showed a correlation with peripheral blood eosinophil counts (p<0.001). Conclusion: These findings demonstrate that FeNO levels are not associated with measures of asthma control in patients treated with ICS. Information on airway inflammation from FeNO concentrations seems to be unrelated to levels of asthma control

• 대한가정학회지
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• 제25권3호
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• pp.63-82
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• 1987

This is the forth consecutive study on the 'Standardization of Measurement for Korean Kitchen Work Centers and Cabinets for Future Design Criteria'. The purpose of the present research was (1) to classify homemakers' representative like styles related to the kitchen, (2) to examine homemakers' present concerns on the kitchen space, and (3) to investigate homemakers' desire and preference on the kitchen space. Twenty two hundred homemakers of upper and middle class residing in Seoul, Busan, Daegu, Incheon, Gwangju and Daejeon were selected for the survey and 1,843 among them were used for data analysis. Data were selected for the survey and 1,843 among them were used for data analysis. Data were analyzed using the SAS program package. The statistics used were frequency, percentage, mean, factor analysis, F-test, Duncan's multiple range test, X2-test, cluster analysis. Findings from the research as follows: (1) Four factors were found as indicaters of the life style; values on the household work, desire on social function of the kitchen, degree on cooperation of family members on the household work, and convenient management. It was noticeable that homemakers had positive responses in terms of desire on social function of the kitchen and degree on cooperation of family members on the household work. Homemakers' life styles related to the kitchen space were classified into 6 categories. (2) Relatively a few respondents answered that the kitchens were very well furnished. If, however, the economic conditions become better, substantial number of them wanted to invest for better kitchen following the one for living room. (3) It was found that most respondents preferred the arrangement of space, where dining and kitchen in one space, and a hard wall or soft treatment was between living and dining/kitchen area. (4) Many respondents desired pantry, utility and laundry area be near the kitchen or in the same space with it, thereby forming a utility core in a housing space.

• 한국초등상담교육학회:학술대회논문집
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• 한국초등상담교육학회 2004년도 학술대회
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• pp.205-222
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• 2004

Fundamental direction of the seventh education curriculum aims to nurture active and creative Koreans for globalized and information-oriented society of 21 century, and emphasizes the importance of career education identifying its subsections that are practices of student-focused education of aptitude, ability and career. Hence, as a clear targeting of career objective from elementary school through relevant career education enables them to recognize appropriate career for themselves, the basis of self- actualization and social contribution must be settled. This paper seeks to identify the relationship between career awareness and self-esteem by examining and analysing differences of sex, region and level. This study selected 536 samples from 4th and 6th year elementary school students in C city and S town of Chungcheong province. Career awareness test and self-esteem test is used as a measurement, statistical process control (SPC) of career awareness and self-esteem (M, SD, r) is made for each variable, and the significance was tested. The result of this research can be summarized as follows: First, generally there is very significant regional differences of career awareness (F=16.817, p<.001), which contains that urban children has higher career awareness (M=120.22) than that of rural children (M=113.87). In subsections of career awareness, self-esteem of urban children is higher than that of rural children, and the attitude & for career and values of girls is higher than that of boys. In terms of the career planning, urban children and 6th year students are higher than rural children and 4th year students. Second, self-esteem generally has very significant regional difference (F=12.123, p<.01), which contains that the self-esteem of urban children (M=106.50) is higher than that of rural children (M=101.80). In comparison of different forms, a very significant difference is as much as p<.01 level (F=11.046), which contains 4th year students (M=105.63) have higher self-esteem than 6th year students (M=102.05). In subsections of self-esteem, gross self-esteem and social self-esteem of urban children are higher than rural children. In terms of family self-esteem, urban children and 4th year students have higher figure than rural children and 6th year students. In school self-esteem, 4th year students had higher level than the 6th. Third, the career awareness and self-esteem are generally related as r= .50, it was very significant at p<. 001 level. All subsections of career awareness had positive relationships except the school self-esteem. According to the result, rural children had relatively low career awareness and self-esteem. This is presumably due to relatively poor circumstances for rural children, comparing to that of urban children. Therefore rural elementary schools should try to let them have appropriate career awareness by associating with family, school and local society to expand the opportunity of experiences for the self perception of aptitude, nature and interest. In addition, we have to strive for the whole-minded education increasing the self-esteem of rural children and giving them positive thinking through career counselling, personal counselling and group counselling.

• 통상정보연구
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• 제16권5호
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• pp.151-178
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• 2014

리히텐슈타인의 글로벌 전동공구 기업 Hilti는 유럽의 미소국에서 작은 가족기업으로 출발하여 태생적인 입지적 약점을 극복하고, 현재 120여 개국에 진출한 건설장비 및 공구산업에서 글로벌 기업으로 자리매김한 하나의 성공사례이다. 또한 Hilti의 글로벌 시장성공은 국제경쟁력 결정요인으로서 요소조건과 수요조건 등 일국의 내생적 조건의 중요성을 설명하는 Porter의 diamond 이론에 배치되는 하나의 사례로 평가될 수 있다. 즉, Hilti의 글로벌 시장 성공사례는 누가 무엇을 얼마나 가지고 출발하느냐가 아니라 주어진 환경조건에서 자신의 역량을 얼마나 효율적으로 활용하느냐에 따라 기업은 타고난 내생적 한계를 극복하고 경쟁적 지위를 확보하는데 더 중요하다는 사실을 확인시켜주고 있다. 본 사례연구에서는 Hilti의 글로벌 경쟁력 및 성공요인을 기업특유자산과 전략특유자산 관점에서 분석하고, 시장성공 요인을 고품질의 제품개발역량, 조직 혁신성, 고객지향에 입각한 마케팅전략 그리고 글로벌화 전략 등으로 제시하였다. Hilti의 글로벌 성공에서 한국 제조 중소기업들에게 제시될 수 있는 시사점은 무엇보다 기업의 혁신 노력과 고객지향에 기반 한 명확한 비즈니스 전략과 전략의 구현이라고 할 수 있다.

• 한국사회복지학
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• 제67권3호
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• pp.31-55
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• 2015

본 연구는 사회복지서비스 제공자들이 이용자로부터 당하는 언어폭력경험이 이직의도에 미치는 영향을 확인하고, 두 변수의 관계에서 이용자-제공자 교환관계(CMX)와 직무만족의 매개효과를 검증하였다. 더불어 해당 변수들의 관계에서 직종 간에 차이가 있는지를 알아보고자 건강가정지원센터 직원들을 일반직과 아이돌봄직으로 구분하여 다집단분석과 잠재평균차이 분석을 시도하였다. 연구목적을 위해 서울 및 경기지역 건강가정지원센터 56곳에서 근무하는 직원들을 대상으로 전수조사하여 319개의 응답을 최종분석에 활용하였고, 결과는 다음과 같다. 첫째, 사회복지서비스 제공자들의 언어폭력경험과 이용자-제공자 교환관계, CMX와 직무만족 그리고 직무만족과 이직의도 간의 관계는 통계적으로 유의미한 것으로 검증되었다. 둘째, 언어폭력경험과 이직의도 사이에서 CMX와 직무만족이 완전매개역할을 하는 것으로 나타났다. 셋째, CMX와 직무만족을 매개로 하여 언어폭력경험이 이직의도에 미치는 구조적 관계에서 직종 간 차이는 없었으나, 언어폭력경험과 CMX의 잠재평균은 통계적으로 유의미한 차이를 보였다. 이러한 연구결과에 따라 사회복지서비스 제공자들에 대한 언어폭력경험의 부정적 영향을 최소화하고 CMX 질을 높이기 위한 실천적 정책적 방안들을 제언하였다.