• 한국노년학
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• 제40권2호
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• pp.239-255
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• 2020

본 연구의 목적은 단독가구 노인의 가족관계가 지역사회참여에 영향을 미치는지 또한 이러한 관계는 성별에 따라 차이가 있는지 고찰하는 것이다. Baltes와 Baltes(1990)의 선택, 적정화, 보상 이론(SOC)과 Prandini(2014)의 가족구성원이 사회적 자본이 된다는 주장을 바탕으로 노년기에 가족관계가 지역사회참여에 영향을 미칠 수 있는지 검증하였다. 이를 위하여 본 연구에서는 한국보건사회연구원에서 실시한 2017년 노인실태조사에서 1인가구를 추출해 연구대상자(남 n=370, 여 n=1770)로 삼았으며, 친구·이웃만남과 경로당·복지관 참여를 종속변수로 다중회귀분석을 실시하였다. 연구결과는 다음과 같다. 남성의 경우 가족관계가 친구·이웃 또는 경로당·복지관 참여에 유의미한 영향을 보이지 않았으나, 여성의 경우는 가족과의 연락빈도가 친구·이웃만남에 정적(+)인 영향을 보였으며, 경로당 복지관 참여에는 가족연락빈도와 자녀관계 만족도가 정적(+) 영향이 나타난 반면 가족만남빈도는 경로당 복지관 참여에 부적(-) 영향이 나타났다. 여성의 경우 가족구성원이 사회적 자본이 된다는 Prandini(2014)의 주장이 지지되는 것으로 보이나, 어떠한 종류의 지역사회참여인가에 따라서 그 영향이 다를 수 있음을 확인하였다. 아울러 이에 대한 실천적 논의와 제언이 제시되었다.

• 한국환경성돌연변이발암원학회지
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• 제22권3호
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• pp.169-174
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• 2002

Resveratrol is known to have potent cancer chemopreventive activity against tumorigenesis caused by 7,12-dimetylbenz［$\alpha$］anthracene(DMBA) which is known to be oxidized to reactive products by cytochrome P450 1B1 (CYP1B1). The effects of resveratrol on the activity of recombinant human P450 1 family enzymes, expressed in Escherichia coli membranes with human NADPH-P450 reductase, were determined by measuring alkoxyresorufin O-dealkylation activity, e.g., ethoxyresorufin O-deethylation (EROD) CYP1A1, methoxyresorufin O-demethylation (MROD), CYP1A2, benzyloxyresorufin-O-debenzylation (BROD), CTP1B1. Resveratrol inhibited CYP1B1 and CYP1A1 activities in a dose-dependent manner with $IC_{50}$/ values of 59 and 10$\mu$M for EROD activity and 1.8 and 30$\mu$M for BROD activity, respectively. Resveratrol had only weak inhibitory effect on CYP1A2 activity ($IC_{50}$/ values of 0.44 mM for EROD and ＞2 mM for MROD). Furthermore, resveratrol did not affect NADPH-P450 reductase activity significantly. Resveratrol inhibited the CYP1B1-dependent EROD activity with a $K_{i}$ of 28 $\mu$M in a non-competitive type manner. these results suggest that resveratrol-derived inhibited of CYP1B1 and CYP1A1 activities may contribute to the suppression of DMBA inducible tumorigenesis observed in extrahepatic tissues.s.

• 부모자녀건강학회지
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• 제18권1호
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• pp.28-38
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• 2015

Purpose: This study was conducted to identify the role and role burden of caregivers with hospitalized children. Methods: The participants included 149 main caregivers from two pediatric hospitals whose children have been hospitalized for at least 2 days at children's hospital. The data were collected from August 1 to september 30, 2013 using self-report questionnaires of checklist type for caregivers' role and Likert scale for role burden. Results: Role for hospitalized children showed that personal hygiene was the highest, and consultation with nurses and doctors was the lowest in total frequency per day. Of the role for the caregivers themselves and family, personal hygiene was the highest, and home management was the lowest. The degree of role burden perceived by hospitalized children's caregivers was the slightly high. Of the categories, emotional burden was the highest and dependent burden was the lowest. There were differences by caregivers' age, children's age, and children's health status. Conclusion: The caregivers performed various roles, especially more frequently on personal hygiene and nutrition for their hospitalized children and themselves and family, and had slightly high role burden. These results may contribute to develop nursing interventions that can help adapt on hospitalization of children and their caregivers.

• Journal of Nutrition and Health
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• 제31권9호
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• pp.1498-1507
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• 1998

The purposes of this study were to : a) examine the current foodservice management practices of twenty-one seniors centers in charge of hoke-delivered meal programs, b) evaluate the attitudes of one hundred and ten recipients of meal service program, and c) provide feedback for the efficient and effective foodservice management for the elderly. Statistical data analysis was completed using the SAS package program for descriptive analysis, T-test, and ANOVA. The results of quantitative analysis indicated that the costs of meals, containers and special foods were mostly dependent on the support from local government(Seoul city). The centers where the volunteers were over seventy five p ercent of the workers were frtty-eight percent and sixty-seven percent of the subjects in food preparation and food delivery to the homebound clients, respectively. Meal preparation and food purchasing were mainly practiced by social welfare worker. Standardized recipes were not developed and meal preparation was controlled under the cook' and volunteers' experiences. The survey results of recipients who participated the home-delivered meals program showed that the mean of meal satisfaction score was rated over three point five in the five-point scale. There were significant differences between dependent variables(volunteer's kindness, plate waste, menu variety) and independent variables(sex, receiving periods and family type of the subjects). (Korean J Nutrition 31(9) : 1498-1507, 1998)

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6263-6267
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• 2012

Background: Glutathione-S-transferase (GST) is an important phase II xenobiotic compound metabolizing enzyme family, involved in tolerance to a particular drug or susceptibility to a diseasec. This study focused the GSTM1 and T1 null allele frequency in the Gujarat population with a comparison across other Inter- and Intra-Indian ethnic groups to predict variation in the possible susceptible status. Methods: DNA was isolated by a salting out method and GSTM1 and T1 homozygous null genotypes were detected by multiplex polymerase chain reaction in 504 unrelated individuals. The genotype distribution of null alleles was compared with Indian and non Indian ethnics reported earlier in the literature using Fisher's test. Results: The frequencies of the homozygous null genotypes of GSTM1 and GSTT1 were 20% (95%CI 16.7-23.9) and 35.5% (95%CI 31.4-39.9) respectively. GSTM1 null frequency did not deviate from most other Indian ethnic groups but differed from the majority of those of non Indian ethnicity studied. The frequency of homozygous null type of GSTT1 was significantly higher and deviated from all Indian groups and a few of non Indian ethnicity. Conclusions: Gujarat ethnicity, possibly the most susceptible for GSTT1 dependent drug disposition and diseases regarding effects of pollution. Further, the results have implications for GSTT1 dependent drugs used for treatment, a serious problem which needs to be solved by physicians and clinical researchers.

• 생약학회지
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• 제33권1호통권128호
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• pp.29-34
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• 2002

Albizzia julibrissin belonging to the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in Oriental traditional medicine. The water extract of A. julibrissin induced apoptosis in Jurkat T-acute lymphoblastic leukemia (ALL) cells as measured by cell morphology. The capability of this herb medicine to induce apoptosis was associated with proteolytic cleavage of specific target protein such as beta-catenin protein suggesting the possible involvement of caspases. The purpose of the present study is also to investigate the effect of A. julibrissin on cell cycle progression. Our results showed that GI checkpoint related gene products (cyclin D1, cyclin dependent kinase 4, retinoblastoma, E2F1) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by A. julibrissin may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.

• 한국해양바이오학회지
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• 제14권2호
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• pp.93-101
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• 2022

Hizikia fusiforme, a type of brown algae, is widely used in Asian cuisine. It has been reported to have various pharmacological effects. In this study, the effects of the ethanol extract from H. fusiforme (EAHF) on the proliferation of human colon carcinoma cells were investigated. The effect on the survival of human hepatocarcinoma and colon carcinoma cells was examined, and results revealed that the anti-proliferative effects of EAHF were higher in colon carcinoma cells than in hepatocarcinoma cells. The inhibition of proliferation of HT29 colon carcinoma cells by EAHF treatment was closely related to the induction of apoptosis. EAHF treatment also increased caspase activity and poly(ADP-ribose) polymerase degradation, induced mitochondrial dysfunction, altered Bcl-2 family protein expression, and increased the rate of cytochrome c released from the mitochondria into the cytoplasm. Furthermore, the production of reactive oxygen species (ROS) was markedly stimulated by EAHF treatment, and when ROS production was blocked, EAHF-induced cytotoxicity was significantly attenuated. These results indicate that the anticancer activity of EAHF in HT29 colon carcinoma cells was induced by ROS-dependent mitochondrial impairment. While EAHF exhibited potent anticancer activity in colon carcinoma cells in this study, further studies on the active components of EAHF and their efficacy should be performed.

• 가족자원경영과 정책
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• 제25권1호
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• pp.63-75
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• 2021

이 연구의 목적은 가족친화 컨설팅 컨설턴트, 인증심사원, 직장교육 전문강사 등 가족친화 전문인력의 활동 경험을 통해 전문인력의 필요 역량을 살펴보고, 전문인력 양성 교육과정 개선 방안을 마련하는 것이다. 이를 위해 10명의 전문인력을 대상으로 포커스그룹 인터뷰를 실시하였다. 본 연구 결과에 따르면, 세 영역에서 활동하는 가족친화 전문인력 모두 가족친화제도 및 가족친화사업 이해라는 공통 역량을 필요로 하지만, 활동 영역에 따른 필요 역량이 상이하므로 공통 교육과 함께 전문인력별로 차별화된 교육과정을 마련하는 것이 필요하다. 교육과정 개편 방향과 관련하여 전문인력별 교육과정 간 연계성 강화, 가족친화 전문인력 교육 시수 확대, 신규 양성교육과 보수교육 간의 차별화가 제안되었다. 이와 함께 교육 운영과 방법의 다양화, 시의성 있는 주제의 심화보수 교육의 필요성 등이 제안되었다. 이러한 결과는 가족친화 전문인력에게 요구되는 역량과 활동 중인 전문인력의 교육 요구에 따라 가족친화 전문인력 교육과정이 재구성될 필요가 있음을 시사한다.

• IMMUNE NETWORK
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• 제20권5호
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• pp.39.1-39.13
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• 2020

Sjögren's syndrome (SS) is a chronic and systemic autoimmune disease characterized by lymphocytic infiltration in the exocrine glands. In SS, type I IFN has a pathogenic role, and recently, inflammasome activation has been observed in both immune and non-immune cells. However, the relationship between type I IFN and inflammasome-associated pyroptosis in SS has not been studied. We measured IL-18, caspase-1, and IFN-stimulated gene 15 (ISG15) in saliva and serum, and compared whether the expression levels of inflammasome and pyroptosis components, including absent in melanoma 2 (AIM2), NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein (ASC), caspase-1, gasdermin D (GSDMD), and gasdermin E (GSDME), in minor salivary gland (MSG) are related to the expression levels of type I IFN signature genes. Expression of type I IFN signature genes was correlated with mRNA levels of caspase-1 and GSDMD in MSG. In confocal analysis, the expression of caspase-1 and GSDMD was higher in salivary gland epithelial cells (SGECs) from SS patients. In the type I IFN-treated human salivary gland epithelial cell line, the expression of caspase-1 and GSDMD was increased, and pyroptosis was accelerated in a caspase-dependent manner upon inflammasome activation. In conclusion, we demonstrate that type I IFN may contribute to inflammasome-associated pyroptosis of the SGECs of SS patients, suggesting another pathogenic role of type I IFN in SS in terms of target tissue -SGECs destruction.

• 생명과학회지
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• 제25권1호
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• pp.1-7
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• 2015

칼슘 의존적으로 활성화되는 neutral protease calpain에 의한 단백질 분해는 세포의 성장을 조절하는데 중요한 단백질들의 역할에 매우 중요한 역할을 한다. Cyclin의 분해는 세포주기의 진행을 위한 필연적인 과정이다. D-type cyclins는 외부자극이나 신호에 의하여 세포주기의 G1 초기에 합성이 된 후 cyclin-dependent kinases (cdk4 및 cdk6)와의 결합하여 세포주기 S기 진입을 촉진하는 역할을 한다. 본 연구에서는 cyclin D3 단백질이 calpain protease에 의하여 번역 후 수준에서 조절 받고 있음을 제시하였다. 본 실험의 조건에서 lovastatin과 actinomycin D가 처리된 PC-3-M 전립선 암세포에서 cyclin D3 단백질의 발현이 완전히 사라졌지만, calpain inhibitor인 LLnL의 처리에 의하여 정상 수준으로 회복되었음을 알 수 있었다. 그러나 26S proteasome의 선택적 억제제인 lactacystin, lysosome 억제제인 ammonium chloride 및 chloroquine, serine protease 억제제인 PMSF는 동일 조건에서 lovastatin과 actinomycin D 처리에 의한 cyclin D3 단백질의 발현저하를 억제하지는 못하였다. In vitro 조건에서 순수 분리된 calpain은 cyclin D3 단백질을 칼슘 농도 의존적으로 분해하였으며, cyclin D3 단백질의 반감기는 LLnL 처리에 의하여 매우 유의적으로 증가되었다. 또한 calpain 저해인자인 calpastatin의 과발현은 PC-3-M 세포에서 뿐만 아니라 NIH 3T3 섬유아세포에서도 cyclin D3 단백질의 반감기 및 안전성을 증대시켰다. 이러한 결과는 cyclin D3 단백질이 칼슘에 의해 활성화 되는 protease calpain에 의해 조절됨을 보여주는 것이다.