• 대한가정학회지
• /
• 제51권1호
• /
• pp.1-16
• /
• 2013

The purpose of this study is to examine the relative influence of various characteristics that affect the happiness and family strength of pre-elderly and the elderly. This study also shows the differences between the two age groups. The National Survey of Korean Families has been done by the Ministry of Equality and Family on 2010. Respondents selected from national survey were 1016 pre-elderly and 629 elderly. The results of this study are as follows; first, 66% of the respondents chose health as the condition is being the important elements of their happiness following money, work (job), children, spouse and family life. Second, the group of people considering work and health as important elements of their happiness is happiest, but the group considering money and health as the important elements of their happiness feels a lower level of happiness. Third, the pre-elderly chose health as the most important condition, but the elderly chose subjective economic condition, among the factors affecting family strength. Fourth, family strength affects most on in regard to the happiness of pre-elderly and the elderly. Next, the more they are financially stable, physically healthier and living in rural areas, they feel the happier they feel. Based on the results of this study, further studies are needed to determine components of family strength that both pre-elderly and the elderly consider as important elements of their happiness. Support programs for the elderly without a spouse will increase the well-being of the elderly.

• 대한가정학회지
• /
• 제46권1호
• /
• pp.117-129
• /
• 2008

In present study, using 1572 low-income families and 266 Head Start teachers from Family and Child Experiences Survey (FACES) in the U.S., we examined the effects of mothers' reading and the characteristics of interaction between teacher-child interaction on 4-and 5-year-olds' cognitive development represented by their verbal and numerical abilities. Frequencies of mothers' reading at home consistently predicted higher scores of children's Peabody Picture Vocabulary Test and Woodcock Johnson Applied Problems. Teachers' sensitivity and permissiveness in their interactions with children in classroom were positively related to children's verbal abilities and teachers' sensitivity predicted better numerical abilities of children after controlling for mothers' reading and the characteristics of family and teacher. The findings shed light on the contributions of teacher behaviors and familial factors to children's cognitive development calling for attention to the need for parent education on cognitively stimulating family environments and continuing education for early childhood teachers focusing on quality interactions with young children.

• 유통과학연구
• /
• 제19권2호
• /
• pp.25-36
• /
• 2021

Purpose: This paper attempts to explore a Christian perspective on the process leading to growth after complex trauma caused by family violence experience. To achieve it, the article tackles the analysis of relationship between the inflictor father and victim, interpersonal relationship, and relationship with God in terms of growth after suffering from the trauma of family violence with a Christian perspective. Research design, data, and methodology: This study employed an in-depth interview as a methodology. Seven Christian adults who have experienced family violence in childhood are selected for the qualitative case study. 58 concepts, 24 low-level categories, and eight high-level categories are derived from each interview case. Results: The results of the case study show that the negative emotion caused by family violence during childhood is likely to lead to narcissistic rage. It is found that the reflection for posttraumatic growth starts with crying to God, simultaneously expressing pain and suffering. Conclusions: The interesting thing is that they are willing to forgive in the process of trauma therapy. It should be noted that the research results also demonstrate that relationship restoration entails the meaning reconstruction in the interpersonal relations.

• 대한예방의학회:학술대회논문집
• /
• 대한예방의학회 1996년도 제48차 추계 학술대회 연제집
• /
• pp.293-294
• /
• 1996

• 한국산학기술학회논문지
• /
• 제16권3호
• /
• pp.1692-1698
• /
• 2015

본 연구의 목적은 가구풍요도가 행복감에 미치는 영향을 파악하고, 이 관계에서 주관적 건강의 매개효과를 검증하는데 있다. 분석 자료는 제8차(2012년) 청소년건강행태온라인조사를 활용하였으며, 조사대상자는 총 74,186명이었다. 본 연구의 결과, 청소년은 자신의 사회경제적 상태는 다소 부유하지 못하다고 느끼고 있는 반면, 자신은 신체적으로 건강하다고 느끼며 대체로 현실에 만족하는 생활을 하고 있는 것으로 나타났다. 연구모형의 분석결과, 가구풍요도가 높을수록 주관적 건강수준이 높아지며, 주관적 건강수준은 청소년의 행복감을 높이는 것으로 나타났다. 즉 주관적 건강은 가구풍요도와 행복감의 관계에서 부분매개효과가 있음이 검증되었다. 본 연구결과를 토대로 청소년의 행복감에 영향을 미치는 요인인 경제 불평등을 줄이고 심리사회적 자원인 행복감에 영향을 미치는 청소년의 건강 수준을 높이기 위해 사회적 차원의 노력과 다차원적인 보건정책의 수립 및 실행이 필요함을 제언하였다.

• Journal of Microbiology and Biotechnology
• /
• 제26권10호
• /
• pp.1661-1667
• /
• 2016

The ginsenoside-hydrolyzing β-glucosidase gene (bgy2) was cloned from Lactobacillus brevis. We expressed this gene in Escherichia coli BL21(DE3), isolated the resulting protein, and then utilized the enzyme for the biotransformation of ginsenosides. The bgy2 gene contains 2,223 bp, and encodes a protein of 741 amino acids that is a member of glycosyl hydrolase family 3. β-Glucosidase (Bgy2) cleaved the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. Under optimal conditions (pH 7.0, 30℃), we used 0.1 mg/ml Bgy2 in 20 mM sodium phosphate buffer (PBS) for enzymatic studies. In these conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 were converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. In pharmaceutical and commercial industries, this recombinant Bgy2 would be suitable for producting ginsenoside Rd and compound K.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권2호
• /
• pp.163-169
• /
• 2012

Interferon regulatory factor 6 (IRF6) gene is a member of the IRF-family, and plays functionally diverse roles in the regulation of the immune system. In this report, the 13,720 bp porcine IRF6 genomic DNA structure was firstly identified with a putative IRF6 protein of 467 amino acids. Alignment and phylogenetic analysis of the porcine IRF6 amino acid sequences with their homologies to other species showed high identity (over 96%). Tissues expression of IRF6 mRNA was observed by RT-PCR, the results revealed IRF6 expressed widely in eight tissues. One SNP (HQ026023:1383 G>C) in exon7 and two SNPs (HQ026023:130 G>A; 232 C>T) in the 5′ promoter region of porcine IRF6 gene were demonstrated by DNA sequencing analysis. A further analysis of SNP genotypes associated with immune traits including IFN-${\gamma}$ and IL10 concentrations in serum was carried out in three pig populations including Large White, Landraces and Songliao Black pig (a Chinese indigenous breed). The results showed that the SNP (HQ026023:1383 G>C) was significantly associated with the level of IFN-${\gamma}$ (d 20) in serum (p = 0.038) and the ratio of IFN-${\gamma}$ to IL10 (d 20) in serum (p = 0.041); The other two SNPs (HQ026023:130 G>A; 232 C>T) were highly significantly associated with IL10 level in serum both at the day 20 (p = 0.005; p = 0.001) and the day 35 (p = 0.004; p = 0.006). Identification of the porcine IRF6 gene will help our further understanding of the molecular basis of the IFN regulation pathway in the porcine immune response. All these results should indicate that the IRF6 gene can be regarded as a molecular marker associated with the IL10 level in serum and used for genetic selection in the pig breeding.

• Journal of Animal Science and Technology
• /
• 제63권4호
• /
• pp.934-953
• /
• 2021

Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권12호
• /
• pp.7657-7661
• /
• 2013

Background: This study concerns uptake and results of colorectal cancer (CRC) screening of government servant as part of the Health Screening Program that was conducted in Brunei Darussalam in 2009. Materials and Methods: Government servants above the age of 40 or with family history of CRC were screened with a single fecal occult blood test (FIT, immunohistochemistry). Among 11,576 eligible subjects, 7,360 (66.9%) returned their specimen. Subjects with positive family history of CRC (n=329) or polyps (n=135) were advised to attend clinics to arrange screening. All the subjects with positive FIT (n=142, 1.9%) were referred to the endoscopy unit for counselling for screening colonoscopy. Results: Overall only 17.7% of eligible subjects attended for screening; 54.9% (n=79/142) of positive FIT, 8.8% (n=29/329) of positive family history of CRC and none with history of polyps (n=0/135). Of these, only 54 patients (50.5%) agreed for colonoscopy, 52 (48.6%) declined as they were asymptomatic, and one was not offered (0.9%) due to his very young age. On screening colonoscopy, 12.9% (n=7) had advanced lesions including a sigmoid carcinoma in situ and six advanced polyps. The other findings included non advanced polyps (n=21), diverticular (n=11) and hemorrhoids (n=26). One patient who missed his screening colonoscopy appointment re-presented two years later and was diagnosed with advanced right sided CRC. All the advanced lesions were detected in patients with positive FIT, giving a yield of 20.5% for advanced lesions including cancers in the 5.1% FIT positive subjects. Conclusions: Our study showed screening for CRC even with a single FIT was effective. However, the uptake rate was poor with just over half of the patients agreeing to screening colonoscopy. Measures to increase public awareness are important. Since one limitation of our study was the relatively small sample size, larger studies should be conduced in future.

• 한국환경생태학회지
• /
• 제25권4호
• /
• pp.498-515
• /
• 2011

2008년 3월부터 10월까지 총 7회에 걸쳐 주왕산국립공원 일대의 식물상을 조사하였다. 주왕산국립공원지역에서 확인된 환경부 지정 멸종위기야생식물종은 둥근잎꿩의비름, 망개나무, 깽깽이풀, 노랑무늬붓꽃과 가시오갈피나무, 솔나리, 연잎꿩의다리 7분류군 이었으며, 식물구계학적 특정식물종은 총 88분류군으로 V등급에 해당하는 분류군은 깽깽이풀, 망개나무, 솔나리 등 8과 9분류군, IV등급에 해당하는 분류군은 승마, 회리바람꽃, 돌마타리 등의 4과 5분류군, III등급에 해당하는 분류군은 애기석위, 가침박달, 등칡 등 16과 18분류군, 2등급으로는 백작약, 나도바람꽃, 개불알꽃 등의 14과 18분류군, 1등급은 늦고사리삼, 홀아비꽃대, 백선 등 30과 38분류군이 확인되었다. 한국 고유 식물종은 호랑버들, 검팽나무, 할미밀망, 노랑갈퀴, 정영엉겅퀴 등의 총 15분류군이 확인되었다. 귀화식물은 닭의덩굴, 나도닭의덩굴, 다닥냉이, 족제비싸리, 컴프리, 수박풀 등의 총 21분류군으로 조사되었다.