• Journal of Animal Science and Technology
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• 제57권12호
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• pp.44.1-44.9
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• 2015

Background: Heat shock proteins play an important role in protection from stress stimuli and metabolic insults in almost all organisms. Methods: In this study, computational tools were used to deeply analyse the physicochemical characteristics and, using homology modelling, reliably predict the tertiary structure of the blunt snout bream (Ma-) Hsp70 and Hsc70 proteins. Derived three-dimensional models were then used to predict the function of the proteins. Results: Previously published predictions regarding the protein length, molecular weight, theoretical isoelectric point and total number of positive and negative residues were corroborated. Among the new findings are: the extinction coefficient (33725/33350 and 35090/34840 - Ma-Hsp70/ Ma-Hsc70, respectively), instability index (33.68/35.56 - both stable), aliphatic index (83.44/80.23 - both very stable), half-life estimates (both relatively stable), grand average of hydropathicity (-0.431/-0.473 - both hydrophilic) and amino acid composition (alanine-lysine-glycine/glycine-lysine-aspartic acid were the most abundant, no disulphide bonds, the N-terminal of both proteins was methionine). Homology modelling was performed by SWISS-MODEL program and the proposed model was evaluated as highly reliable based on PROCHECK's Ramachandran plot, ERRAT, PROVE, Verify 3D, ProQ and ProSA analyses. Conclusions: The research revealed a high structural similarity to Hsp70 and Hsc70 proteins from several taxonomically distant animal species, corroborating a remarkably high level of evolutionary conservation among the members of this protein family. Functional annotation based on structural similarity provides a reliable additional indirect evidence for a high level of functional conservation of these two genes/proteins in blunt snout bream, but it is not sensitive enough to functionally distinguish the two isoforms.

• 한국지역사회생활과학회지
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• 제18권1호
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• pp.147-160
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• 2007

This research challenges the general notion of one-person households of the elderly during widowhood as impoverished, isolated, and vulnerable. Recognizing a high potential vulnerability, however, this research attempts to describe the diverse composition of one-person households of the elderly. For this purpose, relying on 2% sample data from the 2000 census, it examines regional distribution, socio-economic characteristics, and determinants of one-person households of the elderly during widowhood. Socio-economic characteristics of one-person households of the elderly differ by region. Jeju island is distinct in terms of sex and age distribution, and residence area of children. In general, rates of economic activity and self-subsistence are higher in provinces than in cities. Compared to the elderly living with family, the elderly living alone show high rates of economic activity, self-subsistence, and capability of physical activity. Results of logistic regression analysis of determinants of living arrangement are consistent with those of descriptive statistics. Those who are economically active and able to move around without assistance tend to live alone after the death of a spouse. Number of sons and living in an urban area are negatively associated with living alone, whereas females are more likely than males to live alone. According to the separate analysis by age, the positive effect of economic activity is greater in the oldest of the old than in other age groups. Those who possess high educational attainment tend to live alone when they are 80 and older, unlike other age groups. Based on these findings, this paper finds that one-person households of the elderly nay not always be the most vulnerable group, and are diverse in terms of socioeconomic characteristics.

• 환경생물
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• 제22권4호
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• pp.543-549
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• 2004

Ixeris dentata is a typical oriental herb. It is a widely distributed perennial in Korea, Japan and China, which belongs to the Compositae Family. The whole plant of I. dentata has been used for the treatment of pneumonia, contusion, tumor and hepatitis. It has also been used for the treatment of allergic diseases as a folk therapy in Korea. I. dentata is known to have aliphatics, triterpenoids and sesquiterpene glycosides in its composition. The present study was designed to explore the protective effects of water- and ethanol- extracts from I. dentata on irradiated rodents. For oral administration (twice per day), the extractive powder of I. dentata and the positive control (ascorbic acid) were dissolved at a concentration of 0.5 and 250mg $ml^{-1}$ in saline, respectively. Thirty days after irradiation, the ratio of the weight of the testis to the body weight was lower than 50% in the radiation groups than the control group. The ALP concentrations in the group treated with the water-extracts of the leaf were $79.68\pm{1.39%}$ (p<0.05) of those of the radiation control. Both of the SGOT and SGPT in the group treated with the ethanol-extract of the root were $72.68\pm{0.95}\;and\;77.87\pm{5.74}$ (p<0.05) of those of the radiation control, respectively. The levels of DNA damage induced by gamma radiation decreased in the experimental group to which the extracts of I. dentata were administered before irradiation. In conclusion, these results indicate that the extracts of I. dentata have an excellent ability to reduce the radicals and they have a protective effect on DNA breakage caused by radiation.

• 한국어류학회지
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• 제5권2호
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• pp.194-207
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• 1993

1992년부터 1993년까지 군산 연안 어류 군집의 계절적 변동을 조사하였으며, 조사 기간에 채집된 어종은 14목 47과 82속 98종이었다. 저인망에 의해서 채집된 어류 가운데 참서대 Cynoglossus joyneri와 꽃돛양태 Repomucenus ornatipinnis가 연중 가장 많이 출현하였고, 낭장망에 의해 채집된 어류로는 밴댕이 Harengula zunasi와 멸치 Engraulis japonica가 겨울을 제외한 계절에 우점어류로 나타났다. 낭장망에 의해 채집된 어류와 저인망에 의해 채집된 어류의 생체량은 계절에 따라 차이를 보였는데, 전자의 경우 여름철에 후자의 경우 늦봄과 겨울철에 가장 많은 양을 나타냈다. 이러한 결과는 여름철에 양태 Platycephalus indicus, 겨울철에 꼼치 Liparis tanakai가 조사 해역에서 많은 출현량을 나타내기 때문이다. 이들 어류는 특히 산란기에 내만쪽으로 모여든다. 한편 조간대에서는 모두 14종의 어류가 출현하였는데 이들 중 13종은 망둑어과 어류이며 산란기에 다량 출현함으로서 군산 연안은 많은 연안 어류의 산란장으로 여겨진다. 조사 기간 동안에 낭장망, 족대, 저인망, 그 밖의 어망으로 채집한 어류의 계절별 종 수는 봄철에 64종, 여름철에 59종, 가을철에 63종, 겨울철에 27종이었으며 군산 연안 어류의 계절별 출현량 및 종조성 변동은 주로 수온의 영향을 받는 것으로 사료된다.

• 한국실내디자인학회논문집
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• 제24권4호
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• pp.80-90
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• 2015

This study aims to improve plans by analyzing spatial compositions of cohousing common houses, their role depending on the types of users and spaces, and satisfaction from the user-oriented design perspective. For this study, a basic concept was examined based on previous studies and literature reviews. So-Haeng-Ju(Happy House with Communication) Houses 1, 2, and 3 in Seongmisan Village was analyzed. As well, interviews with residents of these houses were conducted to analyze the role of user behavior in common house depending on the types of users and spaces. As a result, it was found that there were common characteristics of roles in an exchange with neighbors, cooperative child-care, and residents' meetings. The time and behavioral types were different depending on the living patterns, characteristics, and family members of residents. User satisfaction with the locations of community facilities including communal kitchen and communal living room, the composition of rooms, communication with residents, and the connection between communal living space and personal living space, was above the average. However, user satisfaction with storage facilities, or defect repair and facility maintenance was low. Most of cohousing common house users were satisfied with the use and size of spaces, and a sense of community, including communication with neighbors beyond the environment and Seongmisan Village. In conclusion, a realistic alternative is required for future cohousing by developing a system regarding human resources networks and cost bearing issues.

• Journal of Microbiology and Biotechnology
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• 제26권5호
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• pp.876-882
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• 2016

Tylosin has been used as a livestock feed additive and antibiotic growth promoter for many years. However, the mode of action by which tylosin enhances animal growth is unclear. We used high-throughput sequencing of 16S rRNA genes to investigate the effects of tylosin as a feed additive on swine gut microbiota. No significant difference in the rate of weight increase was observed between control and tylosin-treated pigs during a 10-week feeding trial. However, tylosin-treated pigs showed rapid increases in the relative abundance of the phylum Firmicutes. Increases in Firmicutes species are associated with (so-called) obese-type gut microbiota. The abundance of species of four families of the phylum Firmicutes (Streptococcaceae, Peptococcaceae, Peptostreptococcaceae, and Clostridiaceae) correlated positively with host weight gain. The abundance of Streptococcaceae family bacteria was least affected by tylosin treatment. Distribution analysis of operational taxonomic units (OTUs) showed that both control and tylosin-treated pigs exhibited similar OTU alterations during growth. However, the tylosin-treated group showed distinctive alterations in gut microbiota when the host weighed approximately 60 kg, whereas similar alterations occurred at around 80 kg in the control group. Our results suggest that use of tylosin accelerates maturation of swine gut microbiota rather than altering its composition.

• 한국실내디자인학회논문집
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• 제19권5호
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• pp.21-28
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• 2010

This study is purposed to understand the spacial usage of the furniture in detail for interior architecture. Many documents have prescribed the spacial furniture at random. While modernist have been interested in the spacial usage of the furniture, the importance of the spacial furniture stands out in architectural space. Since long ago, a great deal of effort has been put into creating a relational harmony between the space and furniture. The villa, the weekend home of the Savoye family, was built between 1928 and 1931. Particularly, the villa Savoye was focused for this study. Le Corbusier viewed desks and case pieces for storage as architecture, and the unite furniture and architecture by developing cabinets in standard modules that could be painted either the wall color to become part of the wall or in contrasting colors to stand out as furniture. Spacial furniture was used architecturally in the interior as well, it gives the space an unexpected playfulness with the color of the finishing materials. The various usage of the Spacial furniture constitute an element of great architectural richness. They have a unique principle based on emotional order and make the man move to another space and experience the spacial connection. In particular, the furniture that was considered by Corbuisier have rational function, division of the space, leaning the structure, and so on. The furniture as the element of the space composition can change the architectural space and view as well as connecting each other. Spacial furniture appear as physical ones called wall, floor, column, and existential value as a living space.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.113-118
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• 1999

The insulin-like growth factor (IGF) is found in all vertebrates and its type-II molecule is regarded as a fundamental embryonic growth factor during development. We have firstly identified, in this study, a cDNA clone corresponding to IGF-II (flIGF-II) from the adult brain of the teleost, Paralichthys olivaceus. We also examined the tissue expression of flIGF-II in several adult tissues by RT-PCR. The flIGF-II cDNA contained a complete ORF consisting of 215 amino acids and one stop codon. Its molecular characteristics appear to be similar to the previously identified IGF-II molecules, in which a common primary structure exhibiting B, C, A, D, and E domains is evidently observed. This cDNA clone seems to be cleaved at $Ala_{52}$ for the $NH_2$-end signal peptide and appears to produce a 98 amino acid-long E-peptide from the $Arg^{118}$. The functional B-D domain regions, therefore, include 65 amino acids and is able to encode a 7.4-kDa protein. The most prominent structural difference between IGF-I and IGF-II was that the D domain of IGF-II exhibits a two-codon-deleted pattern compared to the 8 amino acid-containing IGF-I. The insulin family signature in the A domain and six cysteins forming three disulfide bridges between the B and A domains were evolutionary-conserved from teleosts to mammalian IGF-II. Interestingly, the E-peptide region appears to provide a distinct hallmark between teleosts in amino acid composition. The flIGF-II shows 85.1% of sequence identity to salmon and trout, 90.6% to tilapia, and 98.4% to perch in amino acid level. In tissue expressions of IGF-II, it is very likely that flIGF-II has a significant expression in the adult brain. However, liver seems to be the main source for IGF-II production, and relatively low signals were observed in the adult muscle and kidney. Taken together, it would be concluded that the functional region for IGF-II mRNA is highly similar in phylogeny and is evolutionary, conserved as a mediator for the growth of vertebrates.

• 한국주거학회논문집
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• 제17권3호
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• pp.19-30
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• 2006

The purpose of this study was to compare the characteristics of floor plan of $85m^2$-sized housing units with 2-bay and 3-bay and the residents' satisfaction with them. The subjects for analyzing floor plan were six floor plans of $85m^2$-sized housing units with 2-bay and nine floor plans of housing units with 3-bay in the new town, Hwamyeong-dong, in Busan. The floor plans were analyzed based on seven characteristics of floor plans such as the composition type of entrance core hall, the entrance arrangement, width and depth of the housing unit, size of each room, LDK arrangement, arrangement of family bathroom and private bathroom for husband and wife, and balcony arrangement. The subjects for grasping satisfaction with each housing unit were 400 residents of $85m^2$-sized housing units. A half of them lived in housing units with 2-bay and another half of them with 3-bay in the same area. They answered about the degree of satisfaction with the characteristics of each room and the preferred floor plan type if they move to the same size housing units through a questionnaire. The results were as follows; First, the floor plan with 3-bay had more diverse elements than one with 2-bay. Second, the residents who lived in 3-bay housing unit were younger, higher education, and higher income than the ones in 2-bay. Third, the residents who lived in $85m^2$-sized housing unit were more satisfied with and preferred to 3-bay housing units than 2-bay one.

• 한국사회복지학
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• 제69권1호
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• pp.199-222
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• 2017

본 연구는 중증뇌병변장애인 자녀를 둔 아버지가 경험하는 양육경험을 바탕으로 그동안 사각지대에 있던 중증뇌병변장애인의 생활을 살펴보고, 기존 선행연구에서 주로 다루었던 어머니의 장애자녀 양육경험 연구에서 벗어나 아버지만의 고유한 양육경험을 Giorgi 현상학 연구방법을 이용하여 분석하였다. 연구에는 중증뇌병변장애인 자녀를 둔 아버지 4명이 참여하였고, 일 대 일 심층면담을 통해 자료를 수집하였다. Giorgi(2004)의 현상학 연구방법으로 접근하여 분석한 결과는 다음과 같다. 아버지의 양육경험의 명확한 주제는 '숯 덩어리 가슴', '개똥밭에서 살아나기', '삶의 폐허', '현실에 대항하는 자세', '가족 생활양식의 구성', '업(業)으로서의 자녀', '부성 바로 세우기'였다. 분석 결과를 근거로 장애자녀를 양육하는 아버지의 경험을 통해 장애자녀의 어려움과 구조적 문제들을 논의했고, 사회복지 실천차원에서의 구체적 제언을 하였다.