• The Journal of the Korea Contents Association
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• v.12 no.4
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• pp.108-118
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• 2012

In Korean society entered into an aging society, seeing aging women's lives, the lives of older women through women's eyes means, in a sense, calling the older female audience as the subject. On International Women's Film Festival in Seoul (2006), and now in South Korea opened with the title (2002) through the respective movies understanding 'aging' and the way represented older women will be discussed. shows grandma Marta's a pleasant commotion drew with the villagers, family and friends until openig Lingerie Shop brought in her own sewing skills. challenges social prejudices regarding dementia and the brutality, and reflect the meaning of the 'the provision of care for the elderly' in a circle around bond of the mother-in-law and daughter-in-law There is difference of the producig region of made in Switzerland and made in Japan. Nevertheless, women's solidarity and alone 'late blooming flowers, cut back, even if the theme of encapsulate life through a person as a new subject to buy back more than once on the community have to be made is significant hope.

• Journal of the Korea Convergence Society
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• v.9 no.6
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• pp.133-145
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• 2018

This is a descriptive study on what factors influence the quality of life of early and late middle-aged men. This research is the secondary analysis which was conducted with the data of 170 people and the primary data from 2017 by using SPSS/WIN program. The results show that the main factor is depression in early middle-aged men and social support for late middle-aged men, whose overall ability to explain was 77.8% and 64.7%, respectively. Therefore, this research suggests that multidisciplinary and converged approaches-developing nursing intervention programs based on the factors shown in this paper, creating social environment, strengthening social supportive system and making policies-be required for the enhancement of middle-aged men's quality of life.

• BMB Reports
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• v.35 no.3
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• pp.291-296
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• 2002

Thanatin, a 21-residue peptide, is an inducible insect peptide with a broad range of activity against bacteria and fungi. It has a C-terminal disulfide loop, like the frog skin secretion antimicrobial peptides of the brevinin family. In this study, we tried to find the effect of a number of amino acids between the disulfide bond. Thanatin showed stronger antibacterial activity to Gram negative bacteria than other mutants, except Th1; whereas, the mutant peptides with deletion had higher activity to Gram positive bacteria than thanatin. An increase in the number of amino acid(s) using the alanine residue decreased the antibacterial activity in all of the bacteria. Th1 with deletion of threonine at position 15 ($Thr^{15}$) showed similar antibacterial activity against Gram-negative bacteria, but had higher activity against the Gram positive bacteria. In order to study the structure-function relationship, we measured liposome disruption by the peptides and CD spectra of the peptides. Th1 also showed the highest liposome leaking activity and α-helical propensity in the sodium dodecyl sulfate solution, compared with other peptides. Liposome disruption activity was closely correlated with the anti-Gram positive bacterial activity. All of the peptides showed no hemolytic activity. Th1 was considered to be useful as an antimicrobial peptide with broad spectrum without toxicity.

• Journal of Microbiology
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• v.45 no.5
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• pp.409-417
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• 2007

Burkholderia sp. HY-10 isolated from the digestive tracts of the longicorn beetle, Prionus insularis, produced an extracellular lipase with a molecular weight of 33.5 kDa estimated by SDS-PAGE. The lipase was purified from the culture supernatant to near electrophoretic homogenity by a one-step adsorption-desorption procedure using a polypropylene matrix followed by a concentration step. The purified lipase exhibited highest activities at pH 8.5 and $60^{\circ}C$. A broad range of lipase substrates, from $C_4\;to\;C_{18}$ p-nitrophenyl esters, were hydrolyzed efficiently by the lipase. The most efficient substrate was p-nitrophenyl caproate ($C_6$). A 2485 bp DNA fragment was isolated by PCR amplification and chromosomal walking which encoded two polypeptides of 364 and 346 amino acids, identified as a lipase and a lipase foldase, respectively. The N-terminal amino acid sequence of the purified lipase and nucleotide sequence analysis predicted that the precursor lipase was proteolytically modified through the secretion step and produced a catalytically active 33.5 kDa protein. The deduced amino acid sequence for the lipase shared extensive similarity with those of the lipase family 1.2 of lipases from other bacteria. The deduced amino acid sequence contained two Cystein residues forming a disulfide bond in the molecule and three, well-conserved amino acid residues, $Ser^{131},\;His^{330},\;and\;Asp^{308}$, which composed the catalytic triad of the enzyme.

• Journal of the Korean Home Economics Association
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• v.12 no.2
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• pp.563-580
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• 1974

A Study of the Emotionality of Child and Mother The purpose of this study is primarily to understand the emotional climate of child and mother, which is a significant factor in child study and the home life. A sample was collected from a total of 1,592 of whom 796 are elementary school children with their mothers. Of these 796 children 440 are fom private schools, 190 from public schools in Seoul. The rest are taken from a farming area, 100 kilometers from Seoul. The data was basically obtained through answers to a questionaire distributed in September 1973. In this study, the cross-sectional method, percentage calculation and chi-square test are adapted from factor analysis. With this limited amount of data special attention now, the following conclusions can be drawn from this analysis although special attention should be given in making any kind of generalization about the entire population. 1) Children's emotionality (a) There is a higher percentage of emotionally well-adjusted children as opposed to maladjusted children.(51%>11%) (b) There is no correlation between children's emotionality and the following factors: grade, sex, residential area, school records, and educational level of their mothers. 2) Mother's Emotionality (a) There are more mothers who consider themselves happy than unhappy.(49%>2%) On the other hand a higher percentage of mothers reported feelings of tension and insecurity as compared to those who had feelings of firm security(65%>5%) (b) There is a high correlationshionship between marital adjustment and marital happiness. In other words, the more adjusted, the happier. A high marital happiness has strong influence on the preference of sex, on children's adjustment to their friends, and their present living condition. (c) There is a close relationship between a high marital adjustment and the social development of a child: being fond of fathers, homelife, and the acquaintance with many friends. In the same way, the positive emotionality of a mother in raising her children and their school records are also important. 3) Emotional relationship between a mother and her chid There is a close bond of love and respect between a mother and her child. Moreover, a high frequence of close and open-minded communication exist between them in a family. It is evident that the emotional climate of the mother has a strong and powerful influence on her child.

• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1724-1730
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• 2012

An ${\alpha}$-L-arabinofuranosidase (TmAFase) from Thermotoga maritima MSB8 is a highly thermostable exo-acting hemicellulase that exhibits a relatively higher activity towards arabinan and arabinoxylan, compared with other glycoside hydrolase 51 family enzymes. In the present study, we carried out the enzymatic characterization and structural analysis of TmAFase. Tight domain associations found in TmAFase, such as an inter-domain disulfide bond (Cys306 and Cys476) in each monomer, a novel extended arm (amino acids 374-385) at the dimer interface, and total 12 salt bridges in the hexamer, may account for the thermostability of the enzyme. One of the xylan binding determinants (Trp96) was identified in the active site, and a region of amino acids (374-385) protrudes out forming an obvious wall at the substrate-binding groove to generate a cavity. The altered cavity shape with a strong negative electrostatic distribution is likely related to the unique substrate preference of TmAFase towards branched polymeric substrates.

• Journal of English Language & Literature
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• v.57 no.1
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• pp.73-105
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• 2011

The purpose of this paper is to contend the importance of resurrection of fluid identity and ecological imagination for making the habitable biosphere in Marilynne Robinson's Housekeeping. Ruth as a narrator suggests the future-oriented vision that the environment and nature(mother) can be resurrected, crossing Fingerbone bridge of the boundary line of society/nature as a faithful follower of her aunt Sylvie and becoming the existence with a transparent voice despite of her absence. This novel is to rewrite the American pastoral. Based on the patriarchical way despite of the absence of Edmund Foster, Sylvia's conventional housekeeping is to divide between inside and outside of the house. Nevertheless, Sylvia's relentless efforts to keep her house intact turns out to be fragile. Contrasting with Sylvia, Sylvie's housekeeping is to recognize the continuity of inside and outside. She willingly accepts the reconciliation of the self, the nature and the society. After Ruth and Lucille's staying at night in the lake, they are diverged into going their own way. Ruth accepts Sylvie as a substitute mother. Lucille leaves the house voluntarily and go to her Home Economics teacher, Miss Royce, pursuing the ideal mother of symbolic society. Sylvie and Ruth has the more intimate bond, with their trip to the deserted house in the valley. Ruth meditates on the non-solidity of house and the resurrection of her family. Leaving their house to escape from the town people's legal enforcement, Sylvie and Ruth become transients. Although their history is completed by the drown-death publicly, they always want to visit Lucille's well kept house in Fingerbone. Therefore the method for making Ruth and Sylvie as the existences of ecological imagination return to the real world is to accept the reconciliation of nature and society. This novel is not limited as the binary opposition of vagrance/stability and transience/durability. The significant element of fluid identity can be composed of the interactions with transience and stability.

• 한국노년학
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• v.31 no.3
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• pp.623-640
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• 2011

This study explored the experience of the elderly participants and the community users on the social job program For this, in-depth interviews as focus group interview and individual interview were done for the 21 elderly participants and the staffs, the community users in four social job program for the elderly in C city. Findings of this study as follows: first, the elderly valued the transfomation of their healthier life style as well as the supplementary income from the programme. Second, the elderly got individual benefits as like the healthier life style, more income, pride, recognition of family, and also experienced bond with users and the pride of contributor for the community. Third, they defined the job program as 'paid volunteer service', the care of society for the elderly and the contribution of the elderly for the community. Fourth, the user had disbelief on the elderly at first, they were satisfied with the quality of the commodity and services at last, then they recognized the elderly active and competent. As a result, the social job program for the elderly is good for both the elderly and the community. This result suggest that to make good use of the capability and the will of contribution of the elderly, systematic and sustainable support for the elderly is required.

• Animal Bioscience
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• v.37 no.2_spc
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• pp.396-403
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• 2024

Objective: Monofluoroacetate (MFA) is a potent toxin that blocks ATP production via the Krebs cycle and causes acute toxicity in ruminants consuming MFA-containing plants. The rumen bacterium, Cloacibacillus porcorum strain MFA1 belongs to the phylum Synergistota and can produce fluoride and acetate from MFA as the end-products of dehalorespiration. The aim of this study was to identify the genomic basis for the metabolism of MFA by this bacterium. Methods: A draft genome sequence for C. porcorum strain MFA1 was assembled and quantitative transcriptomic analysis was performed thus highlighting a candidate operon encoding four proteins that are responsible for the carbon-fluorine bond cleavage. Comparative genome analysis of this operon was undertaken with three other species of closely related Synergistota bacteria. Results: Two of the genes in this operon are related to the substrate-binding components of the glycine reductase protein B (GrdB) complex. Glycine shares a similar structure to MFA suggesting a role for these proteins in binding MFA. The remaining two genes in the operon, an antiporter family protein and an oxidoreductase belonging to the radical S-adenosyl methionine superfamily, are hypothesised to transport and activate the GrdB-like protein respectively. Similar operons were identified in a small number of other Synergistota bacteria including type strains of Cloacibacillus porcorum, C. evryensis, and Pyramidobacter piscolens, suggesting lateral transfer of the operon as these genera belong to separate families. We confirmed that all three species can degrade MFA, however, substrate degradation in P. piscolens was notably reduced compared to Cloacibacillus isolates possibly reflecting the loss of the oxidoreductase and antiporter in the P. piscolens operon. Conclusion: Identification of this unusual anaerobic fluoroacetate metabolism extends the known substrates for dehalorespiration and indicates the potential for substrate plasticity in amino acid-reducing enzymes to include xenobiotics.

• Journal of Christian Education in Korea
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• v.69
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• pp.9-44
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• 2022

The purpose of this study is to restore religious education at home. Currently, Korean church education is facing a crisis. First, there is a decrease in the number of children in the church due to the problem of the low fertility rate. Second, the number of young people leaving the church is increasing. As a result, Maneun Church and church schools are being reduced or closed. In order to solve this problem, it is necessary to change from church school-centered education to family-centered faith education. This is because the restoration of faith education is also connected with the restoration of the Korean church in crisis. As an alternative to overcome the current crisis of church education, it is necessary to return to the original form of religious education. In other words, we must return to the way God commanded religious education through parents at home. The most important thing is to overcome and recover from the absence of religious education at home. A Christian home becomes a place of education that fulfills the primary responsibility of religious education for children. God has given his parents the primary authority and responsibility for the religious education of their children. However, amid changes in society and home, the educational function of the home was entrusted to other educational institutions or specialized teachers. Parents of Christian families tend to delegate their children's religious education to church schools by neglecting their educational authority and responsibility. Therefore, the purpose of this study is to reinforce that parents should have a Christian view of faith education and become the main agents of their children's faith education. Parents have the authority, responsibility, and duty as teachers for religious education given by God. The educational authority and responsibility of parents originate from God. God has commanded his parents to bring up their children in faith. Therefore, for parents to become the main agents of their children's religious education, restoration is needed in Christian home education. Therefore, the task of restoring the Christian family as a place of effective Christian education and fulfilling the educational mission of faith that God has given to parents is, first, that parents and the church must recognize the importance of Christian home education anew. Second, parents must have the correct awareness and mission in the Christian view of children. The mission of parents in a Christian home is to teach, train, and admonish their children in the Lord so that they can live with Christian values. Third, the church should actively support home education and form a deep bond between church education and home education.