• Journal of Microbiology and Biotechnology
• /
• 제12권3호
• /
• pp.490-496
• /
• 2002

The production of E. coli WC7 phytase from a recombinant E. coli strain was optimized using a statistical experimental design approach. Two-level complete factorial designs with seven variables were used for the media optimization. In the first optimization step, the influence of disodium succinate, yeast extract, $K_2HPO_4,\;NH_4H_2PO_4,\;MgSO_4$, NaCl, and trace elements on phytase production was evaluated. As a result, disodium succinate, yeast extract, $NH_4H_2PO_4$, NaCl, and the trace elements were found to have a positive influence on the phytase production, while $K_2HPO_4\;and\;MgSO_4$ had a negative influence. In the second step, the concentrations of disodium succinate and yeast extract were further optimized using central composite designs. The maximum phytase activity obtained was 234 U/ml using 15.9 g/1 disodium succinate, 20 g/1 yeast extract, 5 g/1 K_2HPO_4,\;10 g/1 NH_4H_2PO_4,\;1.5 g/1 MgSO_4$, 4 g/1 NaCl, and 1.5 m1/1 trace elements, which was about a 14-fold increase in comparison with that obtained using the basal medium.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVI)
• /
• pp.68-72
• /
• 2005

Leuconostoc citreum is one of the representative strains of Leuconostoc spp. that show fast growth rates in fermented vegetables. Sequential experimental designs including the Plackett-Burman design, fractional factorial design, steepest ascent analysis, central composite design and response surface methodology were introduced tooptimize and improve the medium for Leuconostoc citreum. Fifteen medium ingredients were examined and glucose (20 g/l), yeast extract (12.5 g/l), sodium acetate trihydrate (6.12 g/l), potassium phosphate (42.55 g/l) and dibasic ammonium citrate (4.12 g/l)were chosen as the best components to give a critical and positive effect for cell-growth. The biomass was increased to 2.79 g/l (169%), compared to the 1.65 g/l in MRS medium.

• 한국수소및신에너지학회논문집
• /
• 제31권3호
• /
• pp.293-301
• /
• 2020

This paper describes a numerical study on the improvement of performance of the 2 vane pump impellers. The design of these impellers was optimized using a commercial computation fluid dynamics code and design of experiments. Geometric design variables were defined by the impeller blade angle distribution. The objective functions were defined as the total head, total efficiency and solid material size of the impellers. The importance of the geometric design variables was analyzed using 2^k factorial designs. The interaction between the total head, total efficiency and solid material size, according to the impeller blade angle distribution, is discussed by analyzing the 2^k factorial design results.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권4호
• /
• pp.278-284
• /
• 2004

Leuconostoc citreum is one of the representative strains of Leuconostoc spp. that show fast growth rates in fermented vegetables. Sequential experimental designs including the Plackett-Burman design, fractional factorial design, steepest ascent analysis, central composite design and response surface methodology were introduced to optimize and improve the medium for L. citreum. Fifteen medium ingredients were examined and glucose ($20 g/\ell$), yeast extract ($12.5g/\ell$), sodium acetate trihydrate ($6.12g/\ell$), potassium phosphate ($42.55g/\ell$), and dibasic ammonium citrate ($4.12g/\ell$), were chosen as the best components to give a critical and positive effect for cell-growth. The biomass was increased to ($2.79g/\ell$), (169%), compared to the $1.65g/\ell$ in MRS medium.

• 한국유체기계학회 논문집
• /
• 제15권6호
• /
• pp.58-63
• /
• 2012

This paper presents a numerical study on the performance improvement of axial-flow pump with guide vanes. Design optimization for guide vanes in an axial-flow pump has been studied through the implementation of a commercial CFD code and DOE (design of experiments). We also discussed how to improve the performance of the axial-flow pump by designing the guide vanes. Geometric design variables were defined by the meridional plane and vane plane development of guide vanes. The effect of hub tip ratio analyzed the meridional plane of guide vanes. The importance of the geometric design variables was analyzed using $2^k$ factorial designs. The objective functions for guide vane geometric variables were defined as the total efficiency and the total head at the design flow rate. From the $2^k$ factorial design results, the important design variables were found and the performance was increased in comparison with the base design model.

• 한국해양바이오학회지
• /
• 제6권1호
• /
• pp.31-40
• /
• 2014

The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design $2^6$ in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be $1040{\mu}E/(m^2{\cdot}s)$ light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.

• 응용통계연구
• /
• 제28권5호
• /
• pp.951-963
• /
• 2015

마케팅 분야에서 활용되는 컨조인트 분석은 속성들간의 시너지 효과 혹은 적대적(상충적)인 효과의 존재 여부를 파악하는데 관심이 있다. 즉, 속성들의 모든 주효과와 이인자 교호작용효과의 크기 추정에 관심이 많다. 본 연구에서는 해상도가 V인 균형된 불완전 블록 일부요인설계를 이용함으로써 속성들의 모든 주효과와 이인자 교호작용효과들을 추정 가능하게 하는 설문지 문항의 설계 방법에 관해서 연구를 한다. 전체 응답자들에 대한 설문지 문항들의 총 묶음으로 구성된 설문지 자료를 분석하여 핵심적인 소수 효과들을 선별하고, 유의한 속성효과들로 표현된 적절한 모형을 찾은 다음에, 효율적인 컨셉 최적화를 수행한다.

• Journal of the Korean Data and Information Science Society
• /
• 제20권6호
• /
• pp.1015-1027
• /
• 2009

대수기하학적 접근이란 실험계획에서의 공간 내의 점들 즉, 기하학적 대상인 다양체에 대한 문제를 다항식을 매개로 하여 아이디얼 즉, 대수적 문제로 전환하고자 한 것이라 할 수 있다. 지금까지의 연구는 완전요인실험으로부터 효율적인 부분요인실험을 선택하는 절차에 집중되어 왔다. 본 논문에서는 지금까지 연구 방법의 역의 과정을 추정해 보기로 한다. 한 부분요인실험이 선택되었을 때, 그 실험의 교락구조를 그뢰브너 기저를 구한 후 해석한다. 다음으로 그뢰브너 기저를 생성자로 활용하여 선택된 부분실험의 집합을 구별하기 위한 다항함수인 지시함수를 구하는 절차를 알아보기로 한다. 실제로 몇 가지 부분요인실험을 예로 택하여 그 과정을 수행하였다. 연산은 CoCoA 대수연산 소프트웨어를 이용하였다.

• Journal of Microbiology and Biotechnology
• /
• 제18권12호
• /
• pp.1919-1926
• /
• 2008

Statistical experimental designs; involving (i) a fractional factorial design (FFD) and (ii) a central composite design (CCD) were applied to optimize the culture medium constituents for production of a unique antifreeze protein by the Antartic micro algae Chaetoceros neogracile. The results of the FFD suggested that NaCl, KCl, $MgCl_2$, and ${Na}_{2}{SiO}_{3}$ were significant variables that highly influenced the growth rate and biomass production. The optimum culture medium for the production of an antifreeze protein from C. neogracile was found to be Kalle's artificial seawater, pH of $7.0{\pm}0.5$, consisting of 28.566 g/l of NaCl, 3.887 g/l of $MgCl_2$, 1.787 g/l of $MgSO_4$, 1.308 g/l of $CaSO_4$, 0.832 g/l of ${K_2}{SO_4}$, 0.124 g/l of $CaCO_3$, 0.103 g/l of KBr, 0.0288 g/l of $SrSO_4$, and 0.0282 g/l of ${H_3}{BO_3}$. The antifreeze activity significantly increased after cells were treated with cold shock (at $-5^{\circ}C$) for 14 h. To the best of our knowledge, this is the first report demonstrating an antifreeze-like protein of C. neogracile.

• Korean Chemical Engineering Research
• /
• 제46권4호
• /
• pp.756-763
• /
• 2008

본 연구에서는 잉크젯용 전도성 금속 나노 잉크 개발을 목표로 통계적인 실험과 분석을 진행하여 재현성 있는 고품질의 은 나노 입자를 합성할 수 있는 기술을 개발하고자 하였다. 은 나노 입자는 상용 수계분산제 Daxad19를 이용한 용액 환원침전법을 통해 0.3 M의 고농도로 합성되었다. 합성에 주요한 영향을 주는 6개의 인자를 선정한 후 실험 계획법(Design-of-experiment)을 통해 실험을 수행하였다. 합성된 은 입자는 SEM, TEM, UV-Visible 등의 분석법을 이용하여 입자크기 및 분포와 분산도 등을 측정하였으며 통계 프로그램인 Minitab으로 이를 최적화하였다. 통계적인 실험계획 및 분석은 2차 부분요인분석법(2k-fractional factorial design)과 반응표면분석법인 박스-벤켄법(Box-Behnken design)으로 진행하였다. 이를 통한 합성 최적화로 평균입경 $30nm{\pm}10%$를 가진 구형의 은 나노 입자를 합성하였다. 또한 본 연구에서는 실험 결과 해석을 통해 환원침전법에서의 입자크기 및 형상 제어의 방식도 실험적으로 밝혀냈다.