• Journal of Animal Science and Technology
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• 제64권6호
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• pp.1063-1076
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• 2022

Two experiments were conducted to determine the effect of Hermetia illucens larvae (HIL) as protein and protease on growth performance, blood profiles, fecal microflora, and gas emission in growing pig. In experiment 1, the seventy-two crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial body weight (BW) of 27.98 ± 2.95 kg were randomly allotted to one of four dietary treatments (3 pigs per pen and 6 replicates pen per treatments). The experimental design was a 2 × 2 factorial arrangement of treatments evaluating two diets (Poultry offal diets and HIL diets) without or with supplementing protease. The poultry offal in basal diet has been replaced by HIL. In experiment 2, the four crossbred growing pigs ([Landrace × Yorkshire] × Duroc) with an initial BW of 28.2 ± 0.1 kg were individually accepted in stainless steel metabolism cages. The dietary treatments included: 1) PO- (PO-; poultry offal diet), 2) PO+ (PO- + 0.05% protease), 3) HIL- (3% PO of PO- diet was replacement 3% HIL), 4) HIL+ (HIL- + 0.05% protease). In experiment 1, From weeks 0 to 2, average daily gain (ADG) and feed efficiency (G:F) were significantly increased in the PO diet group compared with the HIL group. From weeks 2 to 4, ADG and G:F were higher for protease group than for non-protease group. At weeks 2 and 4, the PO diet group had lower blood urea nitrogen (BUN) levels than HIL diet group. In experiment 2, crude protein (CP) and nitrogen (N) retention were decreased by HIL diet at weeks 2 and 4. The fecal microflora and gas emission were not affected by HIL and protease. The HIL diet showed lower CP digestibility than PO diet and total essential amino acids digestibility tended to higher in PO diet than HIL diet. In summary, the present study revealed that replacement of the PO protein with the HIL protein and the additive of protease in growing pig diets during the overall experimental period had no negative effect.

• 한국유기농업학회지
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• 제29권4호
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• pp.613-623
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• 2021

This study was conducted to investigate the effect of addition levels of coffee and green tea by products extract including polyphenols through hot water extraction on rumen fermentation. The treatment groups consisted of coffee extract (CO), green tea extract (GR) and mixed extract (MIX), and the addition level was 10 µL, 20 µL and 30 µL of three levels. The experiment consisted of a total of 10 experimental groups including the control group, and a full factorial design was used. The effect of polyphenol addition in coffee and green tea by-products was analyzed through main and interaction effect of statistical analysis. The total polyphenol content of the extracts was 106.15, 79.10 and 185.25 ㎍ GAE/g DM for coffee by-product, green tea by-product and mixture, respectively. Total gas production was significantly lower in the treatment groups than in the control (114.00 mL/gDM) (p<0.05). Methane emission tended to decrease as the polyphenol addition level increased. Moreover, the MIX showed the lowest methane emission when 30 µL was added (p<0.05). Volatile fatty acids showed a significant difference compared to the treatment group as a control (98.06 mM) (p<0.05), but there was no change according to the level of polyphenols. As a result of the main effect and interaction, it is thought that the effect on methane reduction and improvement of rumen fermentation in MIX20 can be expected. In a series of studies, the addition of 20 µL of a blended extract of coffee and green tea by-products is thought to reduce methane to levels that do not inhibit rumen fermentation.

• Animal Bioscience
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• 제35권12호
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• pp.1948-1956
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• 2022

Objective: This study was conducted to evaluate the effects of selenium (Se) source and level on the physiological response, reproductive performance, serum Se level, and milk composition in gestating sows. Methods: A total of 54 multiparous sows (Yorkshire×Landrace) with average body weight (BW), backfat thickness (BF), and parity were assigned to one of five treatments with 10 or 11 sows per treatment using a 2×2 factorial arrangement with one additional treatment in a completely randomized design. Inorganic or organic Se (IS or OS) sources were added to the diet at 0.30 ppm and 0.50 ppm Se. A non-Se-fortified corn-soybean meal basal diet served as a negative control. Treatments were as follows: i) Control: corn-soybean based diet, ii) IS30: control+inorganic Se 0.30 ppm, iii) IS50: control+inorganic Se 0.50 ppm, iv) OS30: control+ organic Se ppm, and v) OS50: control+organic Se 0.50 ppm. Results: At day 21 of lactation, piglet weight and weight gain in the OS treatments were higher than those in the IS treatments (p<0.05). Meanwhile, adding 0.5 ppm Se also resulted in the same significant differences in piglet BW and weight gain (p<0.05). Colostrum and milk Se concentrations increased (p<0.05) with Se level for both Se sources but were greater when sows were fed organic Se (p<0.05). Except for 24 hours postpartum, the Se concentrations were higher when sows were fed organic Se (p<0.05). Sow serum Se content was greater as Se levels increased from 0.3 ppm to 0.5 ppm at day 110 of gestation, 24 hours postpartum and day 21 of lactation (p<0.05). The pig serum Se concentration increased as the dietary Se level increased (p<0.05) and was higher when the sow dietary Se source was organic (p<0.05). Organic Se 0.5 ppm also had the highest serum Se level at two measured points (p<0.05). Conclusion: Consequently, supplementation with organic Se or 0.5 ppm Se in a gestating diet could improve piglet performance, the Se status of sows and piglets and milk composition, but organic Se at 0.5 ppm is optimal.

• Animal Bioscience
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• 제36권3호
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• pp.484-491
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• 2023

Objective: The aim of this trial was to investigate the effect of supplementation and withdrawal of selenium-enriched kale sprouts (SeKS) on productivity and egg Se concentration of laying hens. Selenium from commercial Se-enriched yeast (SeY) was used as a comparative Se source. Methods: One-hundred and eighty 61-week-old laying hens were randomly divided into 5 treatment groups with 4 replicates (9 hens each) in a 2×2+1 Augmented Factorial Experiment in a completely randomized design. The experimental diets were basal diet, basal diet supplemented with 0.2 and 0.4 mg Se/kg from SeKS and SeY, respectively. The 8-week feeding trial was divided into 2 periods, namely the Se supplemental period (week 1 to 4) and the Se withdrawal period (week 5 to 8). Results: Productive performance, egg quality and egg Se concentration of laying hens were not affected by sources of Se (SeKS and SeY) during both, the Se supplemental and withdrawal periods. Egg production and egg Se concentration increased (p<0.05) with increasing levels of Se supplementation. The egg Se concentration increased and reached a peak 1 week after Se supplementation. However, concentration of Se in eggs of hens fed Se from both sources decreased rapidly from the second week of the Se withdrawal period to reach the same egg Se concentration of hens fed the basal diet by the fourth week of the Se withdrawal period. Conclusion: The efficacy of Se from SeKS on productivity and egg Se concentration in laying hens was comparable to commercial SeY. Thus, SeKS can provide an alternate organic Se source for production of Se-enriched eggs.

• Animal Bioscience
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• 제36권7호
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• pp.1083-1090
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• 2023

Objective: This study was conducted to evaluate the short-term effects of dietary selenium supplementation on lactating sows on the physiological response, litter performance, milk composition, and tissue selenium retention in piglets when selenium was provided by different sources and at different levels in a lactation diet. Methods: A total of 48 multiparous sows (Yorkshire×Landrace) with average body weight, backfat thickness, and parity were assigned to one of the four treatments with 12 sows per treatment using a 2×2 factorial arrangement in a completely randomized design. Inorganic or organic Se sources were added to the diet at 0.30 ppm and 0.50 ppm Se. Treatments were as follows: i) IS30, basal diet + inorganic Se 0.30 ppm; ii) IS50, basal diet + inorganic Se 0.50 ppm; iii) OS30, basal diet + organic Se 0.30 ppm; and iv) OS50: basal diet + organic Se 0.50 ppm. Results: At Day 21 of lactation, a high tendency of litter weight (p = 0.08) and litter weight gain (p = 0.09) were observed when sows were fed an organic Se source. The milk Se concentration in the organic Se treatment was higher than that in the inorganic Se treatment at Day 21 of lactation (p<0.05). The serum Se concentrations of sows and piglets at Day 21 of lactation were significantly higher when lactating sows were fed organic Se instead of inorganic Se (p<0.01). During the suckling period, the kidney and muscle Se concentrations of piglets at Day 21 of lactation were significantly higher when the sow dietary Se source was organic (p<0.05). Liver Se concentrations were affected by Se source and level (p<0.05). This also resulted in an interaction response at 21 days of lactation (p<0.05). Conclusion: The supplementation of dietary organic Se in a lactating diet could improve sow feed consumption, piglet performance, milk Se level, and the Se status of sows and piglets.

• 한국가금학회지
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• 제12권2호
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• pp.89-95
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• 1985

항생제인 Spiramycin과 Virginiamycin의 성장촉진 효과를 측정하기 위하여 총 360수의 육용계 (broiler chicken) 초생추를 battery에 수용하여 각각 6주씩 2차에 걸친 사양시험과 4일간의 대사시험을 실시하였다. 1차 실험에서는 고단백(21.9%), 고열량( 3159kca1/kg) 사료를 사용하였으며 180수의 숫병아리를 10수씩 무첨가구 Spiramycin(5ppm)구 및 Virginiamycin(5ppm)구에 각각 6반복씩 완전임의 배치하였다. 2차실험에서는 중단백( 19,95%), 중열량(2931 kca1/kg) 사료를 사용하고 90수의 숫병아리와 90수의 암병아리를 10수씩 분리하여 처리당 암ㆍ수 각각 3 반복씩 배치하였다. 사양시험을 통하여 얻어진 증체량, 사료섭취량, 사료효율 및 폐사율은 처리구간에 통계적 유의차가 없었으나 1, 2차 실험 공히 항생제 B구가 무처리구(1차실험) 또는 항생제 A구(2차실험) 보다 약 3% 높은 증체량을 나타내었다. 사료효율에 있어서도 항생제 B구가 가장 좋은 경향을 보여주었다. 2차실험의 결과에 의하면 증체량, 사료섭취량 및 사료효율에 있어서 암수간에는 유의한(p〈0.01) 차이가 있었다. 대사시험결과 항생제 B구가 타처리구보다 영양소 이용률이 높은 경향을 나타내었으며 특히 1차 실험에 있어서 조지방의 이용률은 타처리에 비해 유의하게 (p〈0.01) 높았고 조섬유이용률도 항생제 A구에 비해 유의하게 (p〈0.05) 높았다. 한편 대사체중당 소장의 무게와 길이는 항생제 B구가 타처리구에 비해 무겁고 긴 경향을 보여주었다.

• 한국초지조사료학회지
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• 제43권3호
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• pp.148-155
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• 2023

This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.010⁴ cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 10⁵ cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.

• 한국심리학회지 : 문화 및 사회문제
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• 제11권4호
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• pp.45-66
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• 2005

이 연구는 Anderson과 Bushman(2002)의 GAM에서 예언하는 공격행동과 공격의도에 대한 폭력적 PC게임의 경험과 공격적 성격특성의 효과를 검증하고, 이 두 변인들의 효과가 발달수준에 따라 차이가 있는지를 확인하기 위하여 상관연구와 실험연구를 실시하였다. 상관연구에서는 남자 344명(중학생 189명과 대학생 155명)에게 폭력적 PC게임에 대한 노출, 공격적 성격특성, 폭력사용에 대한 태도, 공격의도, 그리고 공격행동을 측정하여 이 변인들의 상호관계를 분석하였다. 그 결과 공격행동에 대하여 공격적 성격특성은 설명력이 있는 변인으로 나타났으나 GAM의 예언과는 달리, 폭력적 PC게임에 대한 노출의 설명력은 유의하지 않았으며, 이 두 변인의 효과는 발달수준에 따라 공격행동에 대하여 다르게 영향을 미친다는 것이 확인되었다. 또한, 반복적(장기적)인 폭력적 PC게임의 경험과 공격적 성격특성의 정적 관계도 발견되지 않았다. 실험연구에서는 남자 77명(중학생 29명과 대학생 48명)을 2(PC게임의 유형: 폭력적, 비폭력적) x 2(공격적 성격특성: 상, 하) x 2(발달수준: 중학생, 대학생)의 3요인설계로 된 각 실험조건에 무선 배정하여 공격의도의 차이를 분석하였다. 그 결과 폭력적 PC게임의 경험은 중학생집단에서만 공격의도에 영향을 미치고, 공격적 성격특성은 중학생과 대학생집단 모두에서 공격의도에 영향을 미치는 것으로 발견되었다. 특히 폭력적 PC게임과 공격적 성격특성이 공격의도에 미치는 영향은 연령변화에 따라 다르다는 본 연구의 기본가정이 확인되었다. 결론적으로, GAM이 예언하는 폭력적 PC게임의 효과와 공격적 성격특성의 효과가 부분적으로 지지되었으나, 이 변인들의 효과는 어린 중학생들에게서만 나타남으로써 GAM에 대한 미래의 연구는 발달적 조망에서 보완되어야 할 필요성이 제기되었다.

• Journal of Animal Science and Technology
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• 제65권6호
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• pp.1323-1340
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• 2023

There are several factors that affect the welfare and meat quality of pigs during pre-slaughter transport. Among various factors, the effects of weather conditions and loading density were studied. A total of 3,726 finishing pigs were allotted to one of nine groups arranged in a 3 × 3 factorial design according to the weather conditions (low temperature [LT], under 10℃; normal temperature [NT], 10℃-24℃; high temperature [HT], upper 24℃), and loading density (low density [LD], upper 0.43 m²/100 kg; normal density [ND], 0.37-0.43 m²/100 kg; high density [HD], under 0.37 m²/100 kg). Each treatment group follow as: LTLD, LTND, LTHD, NTLD, NTND, NTHD, HTLD, HTND, HTHD. In terms of carcass composition, pigs had the highest carcass weight and backfat thickness at LT. Comparing the HD transport to the ND transport, the meat quality indicated a lower pH and more drip loss. The incidence rate of pale, soft, exudative (PSE) pork was high in the order of the HD, LD, and the ND transport (20%, 9%, and 2%, respectively). The HT transport showed the lowest pH and greatest L^* value under the given weather conditions. Pigs transported under the HTHD and LTLD conditions had the greatest rates of PSE pork (40% and 20%, respectively). Pigs exposed to HD transport had the shortest laying time and the highest overplap behavior. The LDLT transport pigs had a shorter laying time than the LDNT and LDHT transport pigs. In conclusion, too high or too low density transport is generally not excellent for meat quality or animal welfare, however it is preferable to transport at a slightly low density at high temperature and at a slightly high density at low temperature.

• Animal Bioscience
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• 제36권12호
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• pp.1831-1841
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• 2023

Objective: This study was realized to evaluate the effects of supplementation with blends of water and fat-soluble vitamins on animal performance and carcass traits of young Nellore bulls. Methods: Forty-three Nellore bulls, with an initial weight of 261±27.3 kg and a mean age of 8±1.0 months, were used. Five animals were slaughtered at the beginning of the experiment (reference group), to determine the initial empty body weight of the bulls that remained in the experiment. The remaining 38 bulls were fed ad libitum and distributed in a completely randomized design in a 2×2 factorial scheme, with or without supplementation of water-soluble (B-blend+ or B-blend-) and fat-soluble (ADE+ or ADE-) vitamin blends. Diets were isonitrogenous (120 g of crude protein/kg dry matter [DM] of total mixed ration) and consisted of a roughage:concentrate rate of 30:70 based on total DM of diet. The experiment lasted 170 days, with 30 days of adaptation and 140 days for data collection. At the beginning and end of the experimental period, the bulls were weighed to determine the average daily gain. To estimate the apparent digestibility of nutrients and microbial efficiency, spot collections of feces and urine were performed for five consecutive days. Results: DM, ashes, organic matter, crude protein, ethereal extract, neutral detergent fiber corrected for residual ash and residual nitrogenous, and N intake and apparent digestibility were not influenced by vitamin supplementation, but total digestible nutrients intake and non-fibrous carbohydrates digestibility were influenced by B complex vitamin supplementation. Nitrogen balance, microbial efficiency, and performance data were not influenced (p>0.05) by vitamin supplementation. Conclusion: Vitamin supplementation (a blend of water-soluble and fat-soluble vitamins or their combinations) does not influence the animal performance and carcass traits of young Nellore bulls.