Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Application Effects of Bacterial Inoculants Producing Chitinase on Corn Silage

  • Young Ho Joo (Division of Applied Life Science (BK21Four, Insti. of Agric. & Life Sci.), Gyeongsang National University) ;
  • Seung Min Jeong (Grassland & Forages Division, National Institute of Animal Science, RDA) ;
  • Jiyoon Kim (Division of Applied Life Science (BK21Four, Insti. of Agric. & Life Sci.), Gyeongsang National University) ;
  • Myeong Ji Seo (Division of Applied Life Science (BK21Four, Insti. of Agric. & Life Sci.), Gyeongsang National University) ;
  • Chang Hyun Baeg (Division of Applied Life Science (BK21Four, Insti. of Agric. & Life Sci.), Gyeongsang National University) ;
  • Seong Shin Lee ( Animal Nutrition and Physiology Division, National Institute of Animal Science, RDA) ;
  • Byeong Sam Kang (Department of Plant Medicine, Gyeongsang National University) ;
  • Ye Yeong Lee (Department of Plant Medicine, Gyeongsang National University) ;
  • Jin Woo Kim (Department of Plant Medicine, Gyeongsang National University) ;
  • Sam-Churl Kim (Division of Applied Life Science (BK21Four, Insti. of Agric. & Life Sci.), Gyeongsang National University)
  • Received : 2023.09.20
  • Accepted : 2023.09.25
  • Published : 2023.09.30
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Abstract

This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.0104 cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 105 cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.

Keywords

Acknowledgement

This research was funded (Project No. 821069-03-3-HD020) by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), and Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.

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