• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.495-499
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• 2006

In our previous studies, we demonstrated that Vascular Endothelial Growth Factor (VEGF) enhances bovine oocyte maturation and early embryonic development in serum supplemented media. In this experiment, to determine the synergistic effect of VEGF with serum components on early embryonic development in vitro in cattle, 1 mg/ml polyvinyl-alcohol (PVA) was replaced with foetal bovine serum (FBS) in maturation and culture media. Bovine oocytes were matured in Synthetic Oviduct Fluid (SOF) supplemented with PVA, PVA+5 ng/ml of VEGF, FBS, or FBS+VEGF. Fertilized oocytes were cultured in the same conditions for 8 days. The development of embryos was examined at 48 h post- insemination and on days 6, 7 and 8. The results were analyzed using repeated measures two- factor ANOVA, in which the effects of VEGF and serum were assigned as two factors. The development rate to 4- to 8-cell embryos at 48 h was significantly higher in the PVA+VEGF group than in the PVA group (44.7% and 31.5%, respectively). However, the highest development rate to 4- to 8-cell embryos was obtained from the FBS+VEGF group (58.8%). On day 8, the blastocyst rates were higher in the PVA+VEGF (22.8%), FBS (32.1%, p<0.05) and FBS+VEGF (42.1%, p<0.05) groups than in the PVA group (17.1%). Two- factor ANOVA of the development rates indicates that VEGF had a significant effect, but had no synergistic effect with serum components on early embryonic development. The results of the present study demonstrate that VEGF improves the in vitro developmental competence of bovine oocytes and/or embryos independent of the effect of serum components.

• Journal of Acupuncture Research
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• v.33 no.1
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• pp.47-56
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• 2016

Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell. Methods : Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic effect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear $Factor-{\kappa}B$, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a transfection assay was conducted. Results : 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture. 2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More efficacy was observed in co-culture than in separate culture. 3. Nuclear $Factor-{\kappa}B$ activation was clearly decreased. And co-culture showed much less activation than separate culture. 4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear $Factor-{\kappa}B$ were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Furthermore Bee venom effectively enhances the functions of Natural killer cells.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.3 s.31
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• pp.34-54
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• 2006

The effects of four extracts of medicinal herbs, Rubus coreanum, Acorus calamus, Morus alba and Rehmannia glutinosa on hair growth activity and acnes control were investigated. In the course of screening natural extracts for hair growth, we found that the extract of dried root of Rubus coreanum has the hair growth promoting effect. After topical application of these extracts to the back of C57BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. The growth of dermal papilla cells and mouse vibrissae hair follicle cultured in vitro, however, was not affected by treatment of these extracts. Furthermore these extracts do not possesspotent inhibitory effect on $5{\alpha}-reductase$ I and II activity and anti-bacterial effect on Escherichia coli , Propionibacterium acnes, Pityrosporum ovale, Staphylococcus aureus, Staphylococcus epidemidis, and Candida albicans. RT-PCR analysis showed that these extracts did notinduce mRNA levels of growth factors such as insulin-like growth factor-I, keratinocyte growth factor, hepatocyte growth factor and vascular endothelial growth factor in dermal papilla cells. These results suggest that Rubus coreanum has hair growth promoting effect. However, the effects of these materials on the hair growth promotion are not mediated through inhibition of $5{\alpha}-reductase$ I and II activity, stimulation of hair follicle cells and expression of growth factors in the dermal papilla cells.

• Journal of The Korean Society of Integrative Medicine
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• v.8 no.4
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• pp.163-169
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• 2020

Purpose : This study aimed to investigate the anti-allergic effect of Persicaria perfoliata water extract (PPWE) on IgE stimulated rat basophilic leukemia (RBL-2H3) cell line. Methods : P. perfoliata (L.) H. Gross has been used in traditional medicine as an anti-allergic agent, antipyretic, and diuretic and for respiratory disorders. To analyze the anti-allergic activity of PPWE, release of β-hexosaminidase in RBL-2H3 cells was estimated by enzyme linked immunosorbant assay (ELISA). Also, the cytotoxic effect of PPWE was identified by WST assay, and nuclear factor (NF)-κB and its upstream signaling molecules were assessed by western blot analysis. Results : PPWE treatment significantly attenuated β-hexosaminidase release in a dose dependent manner without any cytotoxicity. PPWE inhibited β-hexosaminidase activity by 38.4±1.2, 36.6±0.6, 32.5±2.2 and 26.5±1.2 at 500, 250, 100, and 50 ㎍/㎖ of PPWE, respectively, compared with the control group. In addition, an analysis of the expression level of NF-κB, an inflammation transcription factor, in RBL-2H3 cells upon IgE stimulation provided reults consistent with the results of β-hexosaminidase release. The phosphorylated status of upstream signaling molecules for transcription factor, mitogen activated protein kinases (MAPKs), was also analyzed. The results showed that PPWE treatment dose-dependently inhibited phosphorylation of extracellular regulatory kinase (ERK) and c-Jun N-terminal kinase (JNK). These results show that PPWE had a strong IgE-mediated degranulation inhibitory effect on RBL-2H3 cells. Conclusion : P. perfoliata ameliorated IgE-mediated allergic reaction via the modulation of MAPK and NF-κB signaling pathway in RBL-2H3 cells. These results indicate that P. perfoliata could be a potential candidate for a treatment strategy against various allergic disorders.

• Journal of Digital Convergence
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• v.16 no.6
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• pp.63-73
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• 2018

The purpose of this study is to investigate the influence of KMS(Knowledge Management System) on the performance of KMS by setting the factors affecting KMS 's management performance as knowledge information factor and management factor. And the moderating effect of evaluation and compensation on knowledge information factor, management factor and management performance. As a result of the analysis, it was analyzed that the knowledge information factor set by the knowledge adaptability, the reliability of knowledge information, and knowledge management process affects the definition of management performance. Among the management factors, organizationalization and cooperation factors influence definition Respectively. In the results of verifying whether assessment and compensation play a moderating role, it is found that knowledge compatibility of knowledge information factor, reliability of knowledge information, support of knowledge management process, knowledge sharing activity of management factor, cooperation has played a moderating role in business performance.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.9
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• pp.2015-2020
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• 2012

The subthreshold characteristics has been analyzed to investigate the effect of two gate in Double Gate MOSFET using impact factor based on scaling theory. The charge distribution of Gaussian function validated in previous researches has been used to obtain potential distribution in Poisson equation. The potential distribution was used to investigate the short channel effects such as threshold voltage roll-off, subthreshold swings and drain induced barrier lowering by varying impact factor for scaling factor. The impact factor of 0.1~1.0 for channel length and 1.0~2.0 for channel thickness are used to fit structural feature of DGMOSFET. The simulation result showed that the subthreshold swings are mostly effected by impact factor but are nearly constant for scaling factors. And threshold voltage roll-off and drain induced barrier lowering are also effected by both impact factor and scaling factor.

• Journal of the Society of Naval Architects of Korea
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• v.50 no.5
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• pp.291-297
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• 2013

Generally, form factor is determined through ITTC method. Determining the form factor from ITTC method includes the assumption that the form factor of a full-scale ship is the same value as its model ship. In other words, the form factor is independent on Reynolds number. However, for the more appropriate prediction of the resistance performance of a full-scale ship, the form factor must be determined with the consideration of the variation attendant on Reynolds number. In this research, several Geosim ship models are adopted to investigate the scale effect, and correlation lines of form factor are improved to suggest the better extrapolation method for the prediction of the form factor of full-scale ship. The corrected form factors using the correlation lines are compared with those determined from the results of low-speed resistance tests. To consider the influence of hull form, the correlation lines are determined for the group of high-speed ships and the group of low-speed ships, respectively. The corrected form factors have shown good agreement among the prediction results from each Geosim ship model to the full-scale ship.

• Journal of the Korean Society of Radiology
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• v.15 no.3
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• pp.281-291
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• 2021

This study aimed to measure the quantitative changes in images according to the use of compressed sensing in expressing the slow flow rate in TOF MRA test using magnetic resonance imaging. This study set different blood flow rate sections by using auto-injector and flow phantom and compared changes in the SNR, CNR, SSIM, and RMSE measurements by different CS factors between TOF with CS and TOF without CS. One-way ANOVA was performed to test the effect on the image induced by the increase of the CS factor. The results revealed that TOF MRA with CS significantly decreased scan time without significantly affecting SNR and CNR compared to TOF MRA with CS. On the other hand, the differences in SSIM and RMSE between TOF with CS and TOF without CS increased as the CS factor increased. Therefore, it is necessary to efficiently reduce scan time by adapting the CS technique while considering the appropriate range of the CS factor. Additionally, more studies are needed to evaluate CS factors and the similarity precision of images further.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.6
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• pp.469-478
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• 2022

WNT signaling plays an important role in cardiac development, but abnormal activity is often associated with cardiac hypertrophy, myocardial infarction, remodeling, and heart failure. The effect of WNT signaling on regulation of atrial natriuretic peptide (ANP) secretion is unclear. Therefore, the purpose of this study was to investigate the effect of Wnt agonist 1 (Wnta1) on ANP secretion and mechanical dynamics in beating rat atria. Wnta1 treatment significantly increased atrial ANP secretion and pulse pressure; these effects were blocked by U73122, an antagonist of phospholipase C. U73122 also abolished the effects of Wnta1-mediated upregulation of protein kinase C (PKC) β and γ expression, and the PKC antagonist Go 6983 eliminated Wnta1-induced secretion of ANP. In addition, Wnta1 upregulated levels of phospho-transforming growth factor-β activated kinase 1 (p-TAK1), TAK1 banding 1 (TAB1) and phospho-activating transcription factor 2 (p-ATF2); these effects were blocked by both U73122 and Go 6983. Wnta1-induced ATF2 was abrogated by inhibition of TAK1. Furthermore, Wnta1 upregulated the expression of T cell factor (TCF) 3, TCF4, and lymphoid enhancer factor 1 (LEF1), and these effects were blocked by U73122 and Go 6983. Tak1 inhibition abolished the Wnta1-induced expression of TCF3, TCF4, and LEF1 and Wnta1-mediated ANP secretion and changes in mechanical dynamics. These results suggest that Wnta1 increased the secretion of ANP and mechanical dynamics in beating rat atria by activation of PKC-TAK1-ATF2-TCF3/LEF1 and TCF4/LEF1 signaling mainly via the WNT/Ca²⁺ pathway. It is also suggested that WNT-ANP signaling is implicated in cardiac physiology and pathophysiology.

• Geomechanics and Engineering
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• v.33 no.2
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• pp.141-154
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• 2023

This study investigated the effect of water retention characteristics between aged and fresh Municipal Solid Waste (MSW) on the stability of the landfill. A series of transient numerical modeling for the slope of an MSW landfill was performed considering the variation of water retention characteristics due to leachate circulation. Four different scenarios were considered in this analysis depending on how to obtain hydraulic conductivity and the aging degree of materials. Unsaturated hydraulic properties of the MSW used for the modeling were evaluated through modified hanging column tests. Different water retention properties and various landfill conditions, such as subgrade stiffness, leachate injection frequency, and gas and leachate collection system, were considered to investigate the pore water distribution and slope stability. The stability analyses related to the factor of safety showed that unsaturated properties under those varied conditions significantly impacted the slope stability, where the factor of safety decreased, ranging between 9.4 and 22%. The aged materials resulted in a higher factor of safety than fresh materials; however, after 1000 days, the factor of safety decreased by around 10.6% due to pore pressure buildup. The analysis results indicated that using fresh materials yielded higher factor of safety values. The landfill subgrade was found to have a significant impact on the factor of safety, which resulted in an average of 34% lower factor of safety in soft subgrades. The results also revealed that a failed leachate collection system (e.g., clogging) could result in landfill failure (factor of safety < 1) after around 298 days, while the leachate recirculation frequency has no critical impact on stability. In addition, the accumulation of gas pressure within the waste body resulted in factor of safety reductions as high as 24%. It is essential to consider factors related to the unsaturated hydraulic properties in designing a landfill to prevent landfill instability.