• Title/Summary/Keyword: FS

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Frame Selection, Hybrid, Modified Weighting Model Rank Method for Robust Text-independent Speaker Identification (강건한 문맥독립 화자식별을 위한 프레임 선택방법, 복합방법, 수정된 가중모델순위 방법)

  • 김민정;오세진;정호열;정현열
    • The Journal of the Acoustical Society of Korea
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    • v.21 no.8
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    • pp.735-743
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    • 2002
  • In this paper, we propose three new text-independent speaker identification methods. At first, to exclude the frames not having enough features of speaker's vocal from calculation of the maximum likelihood, we propose the FS(Frame Selection) method. This approach selects the important frames by evaluating the difference between the biggest likelihood and the second in each frame, and uses only the frames in calculating the score of likelihood. Our secondly proposed, called the Hybrid, is a combined version of the FS and WMR(Weighting Model Rank). This method determines the claimed speaker using exponential function weights, instead of likelihood itself, only on the selected frames obtained from the FS method. The last proposed, called MWMR (Modified WMR), considers both original likelihood itself and its relative position, when the claimed speaker is determined. It is different from the WMR that take into account only the relative position of likelihood. Through the experiments of the speaker identification, we show that the all the proposed have higher identification rates than the ML. In addition, the Hybrid and MWMR have higher identification rate about 2% and about 3% than WMR, respectively.

Genomewide Expression Profile of Forsythia Suspensa on Lipopolysaccaride-induced Activation in Microglial Cells

  • Sohn, Sung-Hwa;Ko, Eun-Jung;Kim, Yang-Seok;Shin, Min-Kyu;Hong, Moo-Chang;Bae, Hyun-Su
    • Molecular & Cellular Toxicology
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    • v.4 no.2
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    • pp.113-123
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    • 2008
  • Microglia, which is the primary immune effector cells in the central nervous system, constitutes the first line of defense against infection and injury in the brain. The goal of this study was to determine the protective (anti-inflammation) mechanisms of forsythia suspense (FS) on LPS-induced activation of BV-2 microglial cells. The effects of FS on gene expression profiles in activated BV-2 microglial cells were evaluated using microarray analysis. BV-2 microglial cells were cultured in a 100mm dish $(1{\times}10^7/dish)$ for 24hr and then pretreated with $1{\mu}g/mL$ FS or left untreated for 30 min. Next, $1{\mu}g/mL$ LPS was added to the samples and the cells were reincubated at $37^{\circ}C$ for 30 min, 1hr, and 3hr. The gene expression profiles of the BV-2 microglial cells varied depending on the FS. The oligonucleotide microarray analysis revealed that MAPK pathway-related genes such as Mitogen activated protein kinase 1 (Mapk1), RAS protein activator like 2 (Rasal2), and G-protein coupled receptor 12 (Gpr12) and nitric oxide biosynthesis-related genes such as nitric oxide synthase 1 (neuronal) adaptor protein (Nos1ap), and dimethylarginine dimethylaminohydrolase 1 (Ddah1) were down regulated in FS-treated BV-2 microglial cells. FS can affect the MAPK pathway and nitric oxide biosynthesis in BV-2 microglial cells.

Characterization of Methanotrophic Communities in Soils from Regions with Different Environmental Settings (다양한 환경조건을 가진 토양의 메탄산화세균 군집 특성)

  • Kim, Tae-Gwan;Park, Hyun-Jung;Lee, Sang-Hyon;Kim, Pyeong-Wha;Moon, Kyung-Eun;Cho, Kyung-Suk
    • Microbiology and Biotechnology Letters
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    • v.40 no.2
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    • pp.152-156
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    • 2012
  • Methanotrophic communities from freshwater wetland (FW), seawater wetland (SW), forest (FS), and landfill soils (LS) around Seoul of South Korea, were characterized using comparative sequence analyses of clone libraries. Proportions of Methylocaldum, Methlyococcus and Methylosinus were found to be greater in FW and SW, while Methylobacter and Methylomonas were more notable in FS and Methylocystis and Methylomicrobium more prominent in LS. Lag periods behind the initiation of methane oxidation significantly varied amongst the soils. Methane oxidation rates were greater in $FW{\geq}LS{\geq}SW>FS$ (p<0.05). Thus, the environmental setting is a significant factor influencing the communities and capabilities of methanotrophs.

Soluble extract of soybean fermented with Aspergillus oryzae GB107 inhibits fat accumulation in cultured 3T3-L1 adipocytes

  • So, Kyoung-Ha;Suzuki, Yasuki;Yonekura, Shinichi;Suzuki, Yutaka;Lee, Chan Ho;Kim, Sung Woo;Katoh, Kazuo;Roh, Sang-Gun
    • Nutrition Research and Practice
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    • v.9 no.4
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    • pp.439-444
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    • 2015
  • BACKGROUND/OBJECTIVES: This study was conducted to investigate the effects of fermented soybean (FS) extract on adipocyte differentiation and fat accumulation using cultured 3T3-L1 adipocytes. MATERIALS/METHODS: 3T3-L1 adipocytes were treated with FS and nonfermented soybean (NFS) extract during differentiation for 10 days in vitro. Oil red O staining was performed and glycerol-3-phosphate dehydrogenase (GPDH) activity was measured for analysis of fat accumulation. Expressions of adipogenic genes were measured. RESULTS: Soluble extract of soybean fermented with Aspergillus oryzae GB107 contained higher levels of low-molecular-weight protein than conventional soybean protein did. FS extract ($50{\mu}g/ml$) inhibited adipocyte differentiation and fat accumulation during differentiation of 3T3-L1 preadipocytes for 10 days in vitro. Significantly lower GPDH activity was observed in differentiated adipocytes treated with the FS extract than those treated with NFS extract. Treatment with FS extract resulted in decreased expression levels of leptin, adiponectin, and adipogenin genes, which are associated with adipogenesis. CONCLUSIONS: This report is the first to demonstrate that the water-soluble extract from FS inhibits fat accumulation and lipid storage in 3T3-L1 adipocytes. Thus, the soybean extract fermented with A. oryzae GB107 could be used to control lipid accumulation in adipocytes.

High Quality Free-Standing GaN Substrate by Using Self-Separation Method (Self-Separation 방법을 적용한 고품질 Free-Standing GaN)

  • Son, Ho Ki;Lee, Young Jin;Kim, Jin-Ho;Hwang, Jonghee;Jeon, Dae-Woo;Lee, Hae-Yong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.29 no.11
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    • pp.702-706
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    • 2016
  • We demonstrated that self-separation FS-GaN (freestanding-GaN) was grown on MELO (maskless epitaxially lateral overgrowth) GaN template by horizontal HVPE (hydride vapor phase epitaxy). Before thick GaN grwoth, MELO GaN template was grown on patterned GaN template by MOCVD (metal organic chemical vapor deposition). The laterally overgrown GaN would consist of a continuous well coalesced layer. The mixed TDD (threading dislocation density) of seed and wing region were $8{\times}10^8cm^{-2}$ and $7{\times}10^7cm^{-2}$, respectively. After thick GaN grown by HVPE, the self-separation between thick GaN and sapphire substrate was generated at seed region. The regions of self-separation for FS-GaN and sapphire were observed by FE-SEM. Moreover, Raman results indicated that the compressive strain of seed and wing regions at FS-GaN substrate were slightly released compared to that of thick GaN grown on conventional GaN template. The optical properties of the FS-GaN substrate were examined by using PL (photoluminescence). The PL exhibited that donor bound exciton and donor acceptor pair were observed at low temperature. The effects on optical and structural properties of FS-GaN substrate have been discussed in detail.