• Asian-Australasian Journal of Animal Sciences
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• 제15권7호
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• pp.1045-1050
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• 2002

The aims of this study were to detect spontaneously occurring apoptosis in cultured porcine ovarian cells, to examine the role of growth hormone (GH), tyrosine kinase (TK), protein kinase G (PKG) and cyclin-dependent kinase (CDK) in the control of this process, and to determine whether the effect of GH on apoptosis is mediated by TK-, PKG- and cdc2-dependent intracellular mechanisms. We studied the action of pGH (10 ng/ml), blockers of TK (genistein, lavendustin, both 100 ng/ml), PKG (Rp-Br-PET-cGMPS, 50 nM; KT5823, 100 ng/ml) and CDK (olomoucine, $1{\mu}g/ml$), as well as combinations of GH with these blockers, on the onset of apoptosis in cultured granulosa cells isolated from antral (3-6 mm) porcine follicles. The functional characteristics of an early apoptotic event, DNA fragmentation, were determined using terminal deoxynucleotidyltransferase (TdT)-mediated dUTP nick end labelling (TUNEL), whilst morphological signs of advanced apoptosis such as pyknosis, chromatin marginalization, shrinkage and fragmentation of nucleus, were detected using routine light microscopy. After culture, some ovarian granulosa cells exhibited DNA fragmentation, which in some cases was associated with morphological apoptosis-related changes (pyknosis, shrinkage and fragmentation of the nucleus). GH significantly reduced the proportion of TUNEL-positive cells. Neither TK nor CDK blockers when given alone, significantly affected the percentage of TUNEL-positive cells although both PKG blockers significantly increased this index. Furthermore, TK and PKG blockers given together with GH, prevented or reversed the inhibitory effect of GH on apoptosis, whilst the CDK blocker olomoucine promoted it. These observations demonstrate apoptosis in porcine ovaries and suggest the involvement of GH, TK, PKG and CDK in the control of this process. They also suggest that the effect of GH on ovarian apoptosis is mediated or regulated by multiple signalling pathways including TK-, PKG- and CDK-dependent intracellular mechanisms.

• 농촌계획
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• 제21권4호
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• pp.27-33
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• 2015

Forest ecosystem is considered as an important stepping stone to minimize the impact of climate change. However, the rapid urbanization has caused fragmentation of forest ecosystem. The fragmentation of forest patch results in edge effect which brings about adverse impacts on forest function and structure. Degradation of forest ecosystem decreases carbon sequestration because edge effect reduces productivity. Therefore, we analyzed the impact of forest edge effect on forest ecosystem carbon stock change in Seongnam-si, Gyeonggi-do. We used connectivity analysis to determine forest edge and core area. The field study sites were selected with considering forest age, density, class and soil type. Secondly, forest carbon stock was calculated with allometric equation. The soil carbon stock was derived from Walkely-Black method. Lastly, Mann-Whitney test was conducted to validate differences between carbon stock in edge and core area. As a result of study, the connectivity analysis was effective to determine forest edge and core. The core and edge of forest patch showed different composition of tree species and soil properties. Carbon stock per tree in the edge area was lower than that in the core area. However, the difference of soil organic carbon content between the edge and core were relatively small. This assessment can be applied for the conservation of forest patch as well as quantitative assessment on the forest carbon stock change caused by fragmentation.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.626-631
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• 2005

Nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) is involved in cell apoptosis, which contributes to luteal regression and luteolysis in some species. In large domestic animals, no direct evidence for the relationship between NO and cell apoptosis in the process of corpus luteum regression is reported. The present study was conducted to investigate the localization of iNOS on porcine corpora lutea (CL) during the oestrus cycle and its relation to cell DNA fragmentation and CL regression. According to morphology, four luteal phases throughout the estrous cycle were defined as CL1, CL2, CL3 and CL4. By isoform-specific antibody against iNOS, the immunochemial staining was determined. Luteal cell DNA fragmentation was determined by flow cytometry. The results showed that no positive staining for iNOS was in CL1 and that iNOS was produced but limited to the periphery of CL2, while in the CL3, the spreading immunochemical staining was found inside the CL. No iNOS positive staining was detected in CL4. Meanwhile, DNA fragmentation increased dramatically when CL developed from CL2 to CL3 (p<0.05). In CL4, higher proportion of luteal cells still had fragmented DNA than that of luteal cells from CL1 or CL2 (p<0.05). These results indicate that iNOS expression is closely related to luteal cell apoptosis and then to luteal regression.

• 터널과지하공간
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• 제23권1호
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• pp.42-53
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• 2013

천공 작업 시 드릴비트 버튼의 타격 속도와 타격 간격은 천공효율을 높이는데 있어 매우 중요한 요소이다. 따라서 본 연구에서는 버튼의 타격 속도 및 간격에 따른 암반 파쇄성능을 분석하기 위하여 홉킨스바 시험기를 이용한 타격시험을 수행하였다. 먼저, 버튼의 타격속도에 따른 암석파쇄 현상을 분석하기 위하여 단일타격 시험을 수행하였고, 수치해석을 통해 단일 타격 시험에 대한 암석의 파쇄과정을 모사하였다. 다음으로 버튼의 타격 간격에 따른 천공효율을 예측하기 위하여 타격 후 설정된 거리만큼 암석 시료를 이동시키고 재차 타격하는 방식으로 다중타격 시험을 수행하였다. 타격시험 후 암석의 천공부피는 레이저 스캐너를 이용하여 측정하였으며, 타격에너지와 천공부피를 통해 천공성능을 계산하였다. 이러한 시험 결과를 바탕으로 직경 102 mm 드릴비트의 1회 타격 시 천공성능을 예측하였다.

• 환경영향평가
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• 제7권2호
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• pp.15-26
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• 1998

In order to assess the present status of the fragmentation of ecosystem and the building of ecological corridor, 79 EISs(Environment Impact Statements) which were conducted in Kyunggido and Kangwondo have been reviewed using 36 analysing factor. The results obtained from this study were as follows: a. The most frequently appearing type of ecosystem fragmentation was 'mountain vs. mountain type in topography' and '2 vs. 2 grades in the degree of green naturality.' b. The most frequently listed fauna in EISs included squirrels and wild rabbits for mammals, grass snake and pit viper for reptiles, and tree frog and true tree frog for amphibians. c. Among the EISs for 50 projects, the construction of ecological corridor mentioned in only 4 projects and other structures such as drainage duct and closed conduit in 14 projects were suggested as ecological corridor. There were no corridors suggested in 32 projects. Thus, in case of using other structures for wildlife animals but ecological corridors, it should be accompanied by incidental facilities for including animal movement.

• 한국환경복원기술학회지
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• 제12권6호
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• pp.123-130
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• 2009

Fragmentation due to land developments causes disturbances and changes of composition in forest vegetation. The purpose of the study was to develop the impact assessment model for quantitative distance or degree of disturbance by land developments. This study conducted a survey about structure and composition of forest vegetation to determine degree of impact from land developments. The results of field survey, there was a difference in structure and composition of forest vegetation such as tree canopy, herbaceous cover, and number of vine and alien species the distances from edge to interior area such as 0m, 10m, 20m, 40m, and over 60m. To assess the disturbance of forest vegetation, the factors selected were the rate of vine's cover and appearance of alien species. The impact assessment model about vine species explained by a distance, forest patch size, type of forest fragmentation, and type of vegetation ($R^2$=0.44, p<0.001). The other model about alien species explained by a distance, type of forest fragmentation, type of vegetation, and width of road (85.9%, p<0.005). The models applied to Samsong housing development in Goyang-si, Gyunggi-do. The vines and alien species in the study area have had a substantial impact on forest vegetation from edge to 20 or 40m. The impact assessment models were high reliability for estimating impacts to land developments. The impact of forest vegetation by development activities could be minimized thorough the adoption of the models introduced at the stage of EIA.

• 생약학회지
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• 제36권2호통권141호
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• pp.88-92
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• 2005

Apoptosis plays an important role in autoimmune chronic (Hashimoto's) thyroiditis, a disorder that often results in hypothyroidism. The goal of this study was to induce apoptosis by the combination of inflammatory cytokines, interferon $(IFN)-{\gamma}$ and tumor necrosis factor $(TNF)-{\alpha}$, and to investigate a potential role of medicinal plants in the thyroid follicular cells (FRTL) in vitro. The apoptosis was evaluated by cellular viability, DNA fragmentation, and terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) assay. Extract of Gamgung-tang (GGT, Glycyrrhizae Radix, black beans, Angelicae Radix, and Cnidii Rhizoma) $(0.3{\sim}9.0mg/ml)$ was shown to maintain the viability of cells treated with $IFN-{\gamma}(100U/ml)$ and $TNF-{\alpha}$ (0.5 ng/ml). FRTL cells were found to undergo DNA fragmentation with the inflammatory cytokines. The extract of GGT inhibited DNA fragmentation in dose-dependent manner. The cells with TUNEL-positive nuclei were detected with $IFN-{\gamma}$ and $TNF-{\alpha}$ treatment. The number of TUNEL-positive cells decreased with the treatment of extract of GGT. These results indicate that medicinal plants inhibit the occurrence of apoptosis in thyroid follicular cells, therefore, may have therapeutic potential in the treatment of autoimmune chronic thyroiditis.

• 한국시뮬레이션학회논문지
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• 제17권4호
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• pp.191-198
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• 2008

6LoWPAN은 IEEE 802.15.4 표준의MAC 및 PHY 계층에서 IPv6 패킷을 전송하는 기술이다. IP 계층과 MAC 계층 사이에 위치한 어뎁테이션 계층에서는 IPv6 패킷의 단편화와 재조립을 수행한다. IETF 6LoWPAN WG의 표준트랙 기술문서인 RFC4944에서 패킷의 단편화와 재조립 기술에 대하여 정의하고 있다. 본 논문에서는 IPv6 패킷의 단편화와 재조립 성능을 평가하는 6PASim의 개발을 제안한다. 6PASim은 상위 계층으로부터 전달되는 IP 패킷을 IEEE 802.15.4 프레임에 실어 전송하는 패킷 송신 모듈과 전송된 프레임을 완벽한 IPv6 패킷으로 재조립하는 패킷 수신 모듈로 구성된다. 6PASim을 이용함으로 써 송, 수신된 데이터의 양과 제어메시지의 사용량을 평가 할 수 있다. 시뮬레이션 결과, SRM 기법이 IRM 기법보다 우수한 성능을 보임을 알 수 있다.

• 한국전산구조공학회논문집
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• 제30권2호
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• pp.145-151
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• 2017

콘크리트 구조물 또는 암석의 해체 공정에서 소음, 진동, 분진 등의 발생을 초래하는 기존의 발파공법을 대신하여 비폭성 파쇄제의 이용을 고려할 수 있다. 본 연구는 유한한 정사각형 콘크리트 구조물을 대상으로 하여 비폭성 파쇄제의 팽창이 준정적으로 균열을 발생, 진전시키는 과정을 유한요소해석으로 예측하고 기존 문헌에 보고된 실험결과와 비교함으로써 해석결과의 정확성을 검증하였다. 또한 비폭성 파쇄제 및 천공 홀의 배치에 따른 영향을 최소요구팽창압 관점에서 분석하였다. 더 나아가서 비폭성 파쇄제가 채워지지 않은 천공 홀이 비폭성 파쇄 공정에 미치는 영향을 분석하고, 분리 조각의 수를 증가시켜 파쇄도를 향상시키기 위한 비폭성 파쇄제 및 천공 홀의 효과적인 배치에 대하여 고찰하였다.

• Animal cells and systems
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• 제16권2호
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• pp.104-113
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• 2012

The nucleolus is a distinct nuclear territory involved in the compartmentalization of nuclear functions. There is some evidence of a relationship between nuclear fragmentation during spermatogenesis and chromatoid body (CB) formation. The CB is a typical cytoplasmic organelle of haploid germ cells, and is involved in RNA and protein accumulation for later germ-cell differentiation. The goal of this study was to qualitatively and quantitatively describe the nucleolar cycle during the spermatogenesis of $Phrynops$ $geoffroanus$ (Reptilia Testudines), and compare this nucleolar fragmentation with CB formation in this species through the use of cytochemical and ultrastructural analysis. Qualitative analysis showed a fragmentation of the nuclear material after pachytene of the first meiotic division in the primary spermatocytes. Quantitative analysis of the nucleolar cycle revealed a significant difference in the number of nucleoli and in the size of the nucleolus between spermatogonia and early spermatids. Using ultrastructural analysis, we recorded the beginning of the CB formation process in the cytoplasm of primary spermatocytes at the same time as when nuclear fragmentation occurs. In the cytoplasm of primary spermatocytes, the CB was observed in association with mitochondrial aggregates and the Golgi complex. In the cytoplasm of early spermatids, the CB was observed in association with lipid droplets. In conclusion, our data show that the nucleolus plays a role in the CB formation process. During spermatogenesis of $P.$ $geoffroanus$, the CB is involved in some important biological processes, including acrosome formation and mitochondrial migration to the spermatozoon tail and middle piece region.