• 대한한방내과학회지
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• 제28권3호
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• pp.502-509
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• 2007

Objectives : Folium perillae (FP) can relieve superficial pathogenic factors to dissipate cold and promote the circulation of qi and regulate the function of the stomach and is often used for interior qi-stagnation. We hypothesized that FP could rescue cerebral ischemia in rats. Methods : The present study was carried out to investigate the effects of FP on cerebral ischemia in terms of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in rats. Finally, lactate dehydrogenase (LDH) release was investigated, too. Results : In this study, treatment with FP elevated rCBF and MABP levels in dose-dependent manner. Pre-treatment with indomethacin, an inhibitor of cyclooxygenase, inhibited rCBF increase induced by FP effectively. However, FP did not affect stability during cerebral reperfusion. Finally, FP significantly inhibited LD H activity in vitro Conclusions : These results suggest that FP is useful to treat patient with diseases related to cerebral ischemia, because FP can increase rCBF and MABP.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.675-678
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• 1999

Fibrinolytic protease activity was detected in the fruit body of Pleurotus sajor-caju using a fibrin plate method. Two fibrinolytic activities (FPI and Ⅱ) were found at the regions of 14.5 and 86.0 kDa by using gel-filtration column chromatography. FPⅡ was identified as an alkaline protease, whereas FPⅠ was a neutral protease. Both were inhibited by phenanthrolin and EDTA, suggesting that they are metalloprotease. Inactivated enzyme activities were restored by adding Co/sup 2+/ or Zn/sup 2+/. Iodoacetate inhibited FPⅠ, but not FPⅡ. Both enzymes cleaved B/sub β/ and γ chains of the human fibrinogen. FPⅡ showed a preference to hydrophobic and bulky residues of nitroanilidine compounds as substrates, whereas FPⅠ preferred positively charged residues.

• 과학교육연구지
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• 제46권1호
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• pp.30-39
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• 2022

이 연구의 목적은 중등학생의 자기조절 과정에서 동화, 갈등, 조절의 세부 과정에 대한 뇌 활성을 비교하고 분석하는 것이다. 자기조절 과제는 생물학적 계통발생 과제로 제시되었고, 뇌활성은 fNIRS로 측정 및 분석되었다. 그 결과 동화 과정과 비교하여 갈등 과정에서 좌측 DLPFC, OFC, FP 영역에서 유의미한 활성이 발견되었고, 조절 과정에서는 DLPFC, VLPFC에서 유의미한 활성화가 발견되었다. 중등학생의 학년이 높아질수록 갈등 과정에서도 DLPFC가 증가하고 동화 과정에서도 VLPFC가 증가한다. 또한, 갈등과 조절 과정을 비교한 결과, 7학년 학생들은 오른쪽 VLPFC에서 유의미한 뇌 활동을 보였고, 9학년 학생들은 조절 과정에서 왼쪽 FP와 DLPFC 영역에서 유의미한 뇌 활동을 보였지만, 11학년 학생들은 이 과정에서 유의미한 뇌 활동을 보이지 않았다. 이러한 결과는 신경학적 연구 방법이 인지 활동과 강의실 교육 상황과 관련된 교육 연구에 적용될 수 있음을 보여준다.

• Toxicological Research
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• 제32권4호
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• pp.337-343
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• 2016

We determined the toxicity of mixtures of ethyl acetate (EA), isopropyl alcohol (IPA), methyl ethyl ketone (MEK), toluene (TOL) and xylene (XYL) with half-maximal effective concentration ($EC_{50}$) values obtained using human hepatocytes cells. According to these data, quantitative property-activity relationships (QPAR) models were successfully proposed to predict the toxicity of mixtures by multiple linear regressions (MLR). The leave-one-out cross validation method was used to find the best subsets of descriptors in the learning methods. Significant differences in physico-chemical properties such as boiling point (BP), specific gravity (SG), Reid vapor pressure (rVP) and flash point (FP) were observed between the single substances and the mixtures. The $EC_{50}$ of the mixture of EA and IPA was significantly lower than that of contained TOL and XYL. The mixture toxicity was related to the mixing ratio of MEK, TOL and XYL (MLR equation $EC_{50}=3.3081-2.5018{\times}TOL-3.2595{\times}XYL-12.6596{\times}MEK{\times}XYL$), as well as to BP, SG, VP and FP (MLR equation $EC_{50}=1.3424+6.2250{\times}FP-7.1198{\times}SG{\times}FP-0.03013{\times}rVP{\times}FP$). These results suggest that QPAR-based models could accurately predict the toxicity of polar and nonpolar mixtures used in rotogravure printing industries.

• 한국식품영양과학회지
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• 제31권1호
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• pp.1-6
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• 2002

강낭콩을 가열한 후 B. subtilis ATCC 51189로 발효시켜 새로운 활성 단백질의 생성여부를 확인하였다. 생강낭콩의 수용성 단백질은 열처리에 의하여 감소하였으며, 발효과정에서 단백질의 양은 변화하지 않았으나, 새로운 아미노산이 합성되었다. 생강낭콩의 수용성 단백질(raw protein, RP)은 분자량 118 kDa의 PHA(phytohemagglutinin)의 전형적인 모습을 보였으나, 열처리한 단백질(heated protein, HP)의 경우, RP의 band는 사라지고, 저분자 peptide로 변화되었으며, 발효과정에 의하여 분자량 18.0 kDa의 새로운 단백질(fermented protein, FP)이 생성된 것을 확인하였다. 또한, RP는 128 HU, HP는 4HU, FP는 32HU의 적혈구응집효과를 보였으며, 위암세포(SNU-1)에 대하여 RP 및 FP는 비교적 높은 농도($IC_{50}$/ $\mu\textrm{g}$/mL)에서 항암활성을 보였다. 그리고, RP 및 FP의 경우 1 $\mu\textrm{g}$/mL에서 림프구 증식효과를 보였고, IFN-${\gamma}$, IL-12의 분비를 촉진하였다. 그러나 HP는 항암효과, 림프구 증식효과, cytokine의 분비촉진효과를 보이지 않았다. 따라서, 강낭콩 lectin은 가열에 의하여 활성물질이 파괴되나, B. subtilis에 의한 발효과정에서 새로운 cytokine 분비촉진물질이 생성되는 것을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.44-49
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• 1999

Cellulase production by fed-batch cultivation of Trichoderma reesei Rut C30 with various initial concentrations of Solka Floc in 1 % wheat bran-containing medium was investigated. The cellulase activity and productivity increased with initial Solka Floc concentration up to 5%. When a total Solka Floc concentration of 90 g/l was used for cellulase production, CMC (carboxymethyl cellulose) and FP (filter paper) activities, productivity, and yield were 359.7 U/ml, 30.61 U/ml, 161 FPU $L^{-1}$ $h^{-1}$, and 340 FPU $g^{-1}$, respectively. It was important to maintain a high cell concentration during cellulase production to obtain high cellulase activity and productivity. Cellulase powder was prepared by ammonium sulfate precipitation: FP activity was 396.7 U/g and CMC activity was 6481 U/g.

• 한국어병학회지
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• 제19권1호
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• pp.83-86
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• 2006

Effect of iron on the extracellular proteolytic activity of live Uronema marium was determined by fluorescence polarization (FP) method. Supplementation of 0.5 and 5.0 μM iron significantly increased caseinolytic activity of live U. marinum. In contrast, supplementation of 50 μM iron showed no significant differences in FP values compared to the control. The present result suggests that iron in cultured water or skin tissue of olive flounder may influence on the penetration and establishment of U. marinum, correlating with modulation of extracellular protease activity of the ciliates.

• 한국임상수의학회지
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• 제15권1호
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• pp.41-45
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• 1998

This study was performed to investigate the serum creative kinase(CK) activity and CK isoenzyme in Jindo dog. Serum CK activity and CK-isoenzyme were analyzed in 53 Jindo dogs of both sexes. The mean value and normal range of serum CK activity were 24.1 lu/$\ell$ and 7-91 lu/$\ell$, respectively, in 29 female dogs, 24.8 lu/$\ell$ and 8-89 lu/$\ell$ in 24 male dogs. The CK activity of the Puppy showed a tendency to be higher than that of the adult. There was no significance between Puppy and adult. Three isoenzymes (CK-MM, CK- BB, and CK-MB) were recognized in serum. The mean percentages of female and male were as follows: 48.31fp and 48.1% for CK-MM, 35.49) and 33.61fp for CK-BB, and 8.2% and 10.1% for CK-MB in the puppy and 46.21% and 46.1 % for CK-MM, 36.3% and 37.6% for CK-BB and 10.5% and 9.5% for CK-MB in the adult.

• Korean Journal of Acupuncture
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• 제27권2호
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• pp.141-157
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• 2010

Objectives : The aim of this study is to examine the effects of acupuncture at PC7(Daereung) on normal human EEG using power spectral analysis. Methods : EEG power spectrum exhibits site-specific and state-related differences in specific frequency bands. In this study, power spectrum was used as a measure of complexity. 30 subjects (16 males and 14 females; averaged age = 23.4 years) were engaged with thirty channel EEG study. Results : In $\alpha$(alpha) band, the power values at Fp1, Fp2, F7, F8 channels(p<0.05) during PC7 treatment were significantly decreased. But, the power values at Fz channel(p<0.05) during non-acupoint treatment was significantly decreased. In $\beta$(beta) band, the power values at Fp1, Fp2 channels(p<0.05) during PC7 treatment were significantly decreased. But, the power values at Fz channel(p<0.05) during non-acupoint treatment was significantly decreased and at F8, TCP2 channels(p<0.05) during non-acupoint treatment were increased. In $\theta$(theta) band, the power values at Fp1, Fp2, F7, F8 channels(p<0.05) during PC7 treatment were significantly decreased. But, the power values at Fz channel(p<0.05) during non-acupoint treatment was significantly decreased. In $\delta$(delta) band, the power values at Fp1, Fp2, F7, Fz, F8 channels (p<0.05) during PC7 treatment were significantly decreased. But, the power values at Fz channel (p<0.05) during non-acupoint treatment was significantly decreased. $\alpha/\beta$ values during PC7 treatment were decreased. $\beta/\theta$ values during PC7 treatment were decreased. Conclusion : These results suggest that acupuncture at PC7 induce the change on $\alpha$(alpha), $\beta$(beta), $\theta$ (theta), $\delta$(delta) wave values being decreased and and bothe $\alpha/\beta$ and $\beta/\theta$ values being decreased respectively. This is considered that acupuncture at PC7 affects the activity of cerebral cortex and endocrine system.

• 대한핵의학회지
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• 제33권3호
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• pp.298-305
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• 1999

목적: PET을 이용하여 도파민운반체를 영상화하는데 유용한 [$^{18}F$]FP-CIT는 그 동안 여러 연구팀들에 의하여 합성이 시도되어졌으나 합성의 난이성 때문에 문제가 되어왔다. 이 연구에서는 새로운 방법에 의하여 유효비방사능이 높은 [$^{18}F$]FP-CIT를 높은 수율로 합성하고자 하였다. 대상 및 방법: [$^{18}F$]Fluoropropyl기를 도입하기 위하여 사용된 3-bromo-1-[$^{18}F$]Fluoropropane은 THF 용매 하에서 3-bromo-propyl-1-triflate $5{\mu}L$와 $nBu_4N^{18}F$를 $80^{\circ}C$에서 2분 동안 가열하여 얻어졌으며 정제하지 않고 직접 알킬화반응에 사용하였다. 이렇게 생성된 3-bromo-1-[$^{18}F$]fluoropropane에 nor-${\beta}$-CIT를 넣고 DMF $5{\sim}6$방울이 함유된 아세토니트릴을 반응용매, $Et_3N$을 생성된 산의 중화제로 사용하여 140f에서 20분동안가열하였다. 생성된 [$^{18}F$]FP-CIT는 HPLC에 의해 정제되고 13% 에탄올이 함유된 생리식염수를 사용하여 제제화하였다. 정도관리는 HPLC 및 방사능 박층크로마토그라피에 의한 분석, pH, 발열성 검사로 이루어졌다. 결과· 3-Bromo-1-[$^{18}F$]Fluoropropane은 3-bromopropyl-1-triflate의 triflate기를 $^{18}F$으로 치환하여 $77{\sim}80%$의 높은 방사화학적 수율로 얻어졌으며 같은 반응용기에 nor-${\beta}$-CIT를 넣고 $140^{\circ}C$의 반응온도에서 아세토니트릴과 최소량의 DMF를 사용하여 알킬화반응을 실행하였다. 이때 DMF의 높은 극성과 비점 때문에 HPLC로 정제할 때 문제가 되었으므로 초기에 0.8% $Et_3N$을 포함한 물만을 사용하는 정제방법을 개발하였다. [$^{18}F$]FP-CIT의 동정은 비방사성 동일물질인 FP-CIT와 동시에 HPLC에 주입하여 확인되었다. [$^{18}F$]FP-CIT의 정도관리를 실행한 결과, 방사화학적 순도는 99% 이상, 유효비방사능은 $37GBq/{\mu}mol$ 이상으로 우수하였으며 총 감쇠보정된 방사화학적 수율도 $5{\sim}10%$로 높았다. 최종생성물의 pH는 약 7.0이었으며 발열물질은 없었다. 결론: 이 연구에서는 [$^{18}F$]fluorination 반응의 전구물질로서 3-bromopropyl-1-triflate를 사용하였고 HPLC 정제 조건을 새롭게 개발함으로써 유효비방사능이 높고 비교적 수율도 높은 [$^{18}F$]FP-CIT를 합성하는 새로운 방법을 확립하였다. 이 [$^{18}F$]FP-CIT는 뇌 도파민운반체의 체내 연구에 유용하게 사용될 것으로 사료된다.