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Effect of iron on the proteolytic activity of live Uronema marinum (Ciliata: Scoticociliatida) measured by fluorescence polarization  

Lee, Eun-Hye (부경대학교)
Kwon, Se-Ryeon (부경대학교)
Choi, Seung-Hyuk (부경대학교)
Kim, Ki-Hong (부경대학교)
Publication Information
Journal of fish pathology / v.19, no.1, 2006 , pp. 83-86 More about this Journal
Abstract
Effect of iron on the extracellular proteolytic activity of live Uronema marium was determined by fluorescence polarization (FP) method. Supplementation of 0.5 and 5.0 μM iron significantly increased caseinolytic activity of live U. marinum. In contrast, supplementation of 50 μM iron showed no significant differences in FP values compared to the control. The present result suggests that iron in cultured water or skin tissue of olive flounder may influence on the penetration and establishment of U. marinum, correlating with modulation of extracellular protease activity of the ciliates.
Keywords
Uronema marinum; Protease; Iron; Fluorescence polarization;
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