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Expression Analysis of Flower Color Related Genes in Spray-type 'ARTI-purple' Developed by Gamma-ray Mutagenesis (감마선 변이체 스프레이 국화 'ARTI-purple'의 화색 관련 유전자 발현 분석)

  • Sung, Sang Yeop;Lee, Yu-Mi;Kim, Sang Hoon;Ha, Bo-Keun;Kang, Si-Yong;Kim, Jin-Baek;Kim, Hong Gi;Kim, Dong Sub
    • Journal of Radiation Industry
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    • v.6 no.2
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    • pp.147-152
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    • 2012
  • Anthocyanins are major plant pigment and produced through phenylpropanoid pathway. In this study, anthocyanin biosynthesis mechanisms of chrysanthemum flowers were studied using 'Argus' and flower color mutant 'ARTI-purple' which were induced by 40 Gy gamma irradiation ($Co^{60}$). And, three chrysanthemums, 'Ford', 'Yeonja' and 'Orando' were additionally used as the check varieties to understand the relationship between flower color and expression patterns of genes. The expression patterns of the anthocyanin biosynthetic genes were matched with the flower color of the check varieties. High anthocyanin concentration of 'Orando' showed the high expression of anthocyanin biosynthetic genes. In the white flower of 'Ford', expressions of CHI, DFR and ANS were not identified. Despite different flower color, 'Argus' and 'ARTI-purple' showed different expression patterns compared with the check varieties. From the dot blot analysis, we screened the seven genes showing the different expressions between 'Argus' and 'ARTI-purple'.

Comparison of Continuous Appositional Suture Patterns for Cystotomy Closure in Ex Vivo Swine Model

  • Sang-hun Park;Joo-Myoung Lee;Hyunjung Park;Jongtae Cheong
    • Journal of Veterinary Clinics
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    • v.39 no.6
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    • pp.353-359
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    • 2022
  • Several suture patterns can be used for cystotomy closure, and a continuous suture pattern is the most commonly used. In this study, the fluid-tight ability and other suitabilities of continuous appositional sutures, such as the simple continuous suture pattern (SC), running suture pattern (RN), and Ford interlocking suture pattern (FI), were compared for cystotomy closure. Cystotomy closure was performed using each suture method in 10 cases of ex vivo swine bladders in each group. Suture time, leakage site, suture length, bursting pressure (BP), bursting volume (BV), and circular bursting wall tension (CBWT) were measured. Suture time and suture length were the shortest in RN and the longest in FI. Leakage occurred in two places: the incision line directly and the hole made by the suture. Leakage occurred through the incision line in 4 bladders of the RN group and 2 bladders of the FI group, but not in the SC group, and in the rest of the bladders, leakage occurred through the suture hole. The values of BP, BV, and CBWT increased in the order of FI, SC, and RN. Suture time and suture length can be considered as factors related to healing and side effects. In this study, leakage through the incision was found in a less appositional area; therefore, leakage through the hole could be considered an indicator of better apposition. Good apposition is one of the conditions required for ideal cystotomy closure. The bursting strength representing the fluid-tight ability can be expressed as the CBWT. RN is expected to be efficient and cause a small degree of foreign body reaction; however, it is expected to be less stable. FI has the greatest fluid-tightness ability, but it has been proposed that side effects due to foreign body reactions most frequently occur in FI. In conclusion, SC, which is expected to have a sufficient degree of fluid-tightness and appropriate recovery, is preferable to other continuous appositional suturing methods for cystotomy closure.

POSSIBILITIES TO IMPROVE TRANSIENT GEAR SHIFT NOISE (SHIFT CLONK) IN A PASSENGER CAR

  • BIERMANN J. W.;REITZ A.
    • International Journal of Automotive Technology
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    • v.6 no.1
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    • pp.23-28
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    • 2005
  • The presented investigation of shift clonk in a vehicle with front-wheel drive shows how a detailed analysis of the complete acoustic system with respect to excitation, transfer and radiation foremost enables possibilities of noise reduction to be worked out. One of the most important basics for the shift clonk analysis was a synchronous measurement of both, torsional vibrations in the drive train on the excitation side as well as airborne and structure-borne noise signals on the transfer and radiation side. Thus, root causes could be identified and improvement measures of the internal shift system could be worked out. An analysis of the transfer paths by means of airborne and structure borne noise measurements made evident that the side shafts were responsible for the disturbing frequencies in the transfer paths. With the help of the FE-simulation it was possible to develop measures of structure optimisation for the side shaft system. The realisation of these measures clearly reduced the shift-noises in the vehicle interior.

THE REQUIREMENTS ON STATISTICAL TECHNIQUES IN QS-9000

  • YAMADA Shu
    • Proceedings of the Korean Society for Quality Management Conference
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    • 1998.11a
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    • pp.429-434
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    • 1998
  • QS-9000 was established by Chrysler, Ford, General Motors and some Truck Manufactures to solve problems of efforts to prepare original standards for each company. When we compare the requirements in QS-9000 with those in ISO 9000, we may observe that those of QS-9000 are more specific, since the user of the standard is limited to the automobile industry. In particular, the requirements on statistical techniques are described in more detail. In this paper, we present the requirements on statistical techniques in QS-9000. Specifically, we review the contents of QS-9000 and explore the philosophy and the minimum requirement on statistical techniques behind the description.

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New Parameterizations for Multi-Step Unconstrained Optimization

  • Moghrabi, I.A.;Kassar, A.N
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • v.3 no.1
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    • pp.71-79
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    • 1999
  • We consider multi-step quasi-Newton methods for unconstrained optimization. These methods were introduced by Ford and Moghrabi [1, 2], who showed how interpolating curves could be used to derive a generalization of the Secant Equation (the relation normally employed in the construction of quasi-Newton methods). One of the most successful of these multi-step methods makes use of the current approximation to the Hessian to determine the parameterization of the interpolating curve in the variable-space and, hence, the generalized updating formula. In this paper, we investigate new parameterization techniques to the approximate Hessian, in an attempt to determine a better Hessian approximation at each iteration and, thus, improve the numerical performance of such algorithms.

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생산工程의 自動化에 關하여 (I)

  • 이봉진
    • Journal of the KSME
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    • v.20 no.1
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    • pp.31-39
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    • 1980
  • Automation(자동화)은 1946년 Ford 자동차회사에서 출생한 이래 반도체공업의 기술정보를 배 경으로 한 Computer, NC, Digital 정보처리기술, 도형정보처리기술등의 개발로 급격히 발전하 였다. 1970년대에 접어들면서 공업계기의 발달로 계장 system의 Hardware가 성능이나 신뢰성이 실용화레벨에 올라서 Roqort, 인공화능, man-machine system까지도 나타나게 되었다. 생산 system의 자동화에 있어서도 생산공정의 소재관리, 기계가공, 조립, 검사, 포장등을 일관서있는 연속작업이 되도록하는 종래의 Machanical Automation이 있다. 이는 소품종 다량생산체제에서 고능율, 고도의 가공효율의 요구에 따라 제품의 품질관리 및 제조를 자동전용기에 의한 양산체 제라 하겠는데 이것이 선진국의 1950년대의 자동화의 형태라 할수 있다. 1960년대에 들어서 앞서 기술한 전기 전자 기술의 발달과 제품에 대한 사회의 요구와 그것에 부응하기 위하여서는 생산 Line의 자동화가 점차 지능화하는 경향을 보이고 있으며 이를 Mechatronical Automation이라 부르기도 하나 이 점이 종래의 자동화와 성격이 다르다고 하겠다. 여기서는 생산시스템의 자동 화에 대한 새로운 경향이 어떠한 것이며 또 앞으로의 전망등을 살펴보기로 한다.

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ENGINE CONTROL USING SPEED FEEDBACK

  • Stotsky, A.;Solyom, S.;Kolmanovsky, I.V.
    • International Journal of Automotive Technology
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    • v.8 no.4
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    • pp.477-481
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    • 2007
  • In this article we present a new, reference model based, unified strategy for engine control. Three main modes are considered: first is the driver control mode where the driver controls the engine via the pedal position; second is the dashpot mode, that is, when the driver takes his foot off the pedal; and, lastly is the idle speed control mode. These modes are unified so that seamless transitions between modes now becomes possible. The unification is achieved due to the introduction of a reference model for the engine speed whereby only the desired engine speed is different for different modes while the structure of the control system remains the same for all the modes. The scheme includes an observer that estimates unknown engine load torque. A proof of robustness with respect to unknown load disturbances both within the operating modes and during intermode transitions is given.

Solving a Path Assignment Problem using s-t Cuts (그래프의 s-t 절단을 이용한 경로 배정 문제 풀이)

  • Kim, Tae-Jung
    • Journal of the Korean Society for Precision Engineering
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    • v.26 no.2
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    • pp.141-147
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    • 2009
  • We introduce a noble method to find a variation of the optimal path problem. The problem is to find the optimal decomposition of an original planar region such that the number of paths in the region is minimized. The paths are required to uniformly cover each subregion and the directions of the paths in each sub-region are required to be either entirely vertical or entirely horizontal. We show how we can transform the path problem into a graph s-t cut problem. We solve the transformed s-t cut problem using the Ford-Fulkerson method and show its performance. The approach can be used in zig-zag milling and layerd manufacturing.

A NETWORK MODEL FOR NAVAL OFFICER MANPOWER PLANNING

  • Cho, Doug-Woon
    • Journal of the military operations research society of Korea
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    • v.2 no.1
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    • pp.145-162
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    • 1976
  • For a large organization such as a military service which can foresee future growths in its manpower requirements, a systematic tool that can provide analysis of its present manpower structure and policies in terms of meeting the future requirements, is in order today. This paper proposes a network model for such a purpose. The ROK Navy officer corps manpower system is studied and formulated as a network model, which may be expressed as a linear programming problem of minimizing total cost. An appropriate cost concept is developed and the out-of-kilter algorithm of Ford and Fulkerson is computer programmed to be used as a solution procedure for this network problem. A case study is conducted with a set of hypothetical data on a possible Navy combat-line specialty manpower problem.

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自動化의 重要性

  • 손명환
    • Journal of the KSME
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    • v.20 no.2
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    • pp.81-83
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    • 1980
  • 자동화라는 것은 1788년에 유명한 James Watt의 증기기관에 있어서 조속기에서 처음으로 찾아볼 수 있다고 하며, 그후도 각종기기에서 부분적인 자동제어화된 예를 볼 수가 있다. 그러나, 현대 적이고 본질적인 자동화는 1914년에 Ford 자동차직립공장에서 흐름적립선을 설치하여 다량생산 방식의 근대산업을 개발하여 생산과정을 진정한 의미로 기계화하고 설인간화하였는데 있다고 하며 현대적감독의 자동화라는 말도 1946년에 출현한 transfer machine에 대하여 사용한 것이 처음이라고 한다 여하간 모든 산업에 있어서의 자동화는 현대산업발전의 촉진제로서 역할을 하여 획기적인 변혁을 가져왔고 경제발전의 원동력으로서의 자리를 굳혔을 뿐 아니라 설안간화, 성 력화의 단계를 넘어서 무인화의 영역에 도전하기에 이르렀다. 자동화 무인화의 영역에 도전하 기에 이르렀다. 자동화의 기술은 초기의 기계적방법을 거쳐서 유압식, 전기전자식으로 발전하였 으며, 현대과학기술의 정화인 전산기와 결합되어 종합과학기술로 발전하였고 앞으로 더욱 가속 화될 것이 기대된다.

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