• Journal of Microbiology
• /
• 제40권4호
• /
• pp.305-312
• /
• 2002

The effects of two natural polyamines (putrescine and spermidine) on the synthesis of ArcA, a response regulator of the Arc two-component signal transduction system, were studied using an E. coli mutant deficient in polyamine biosynthesis. Endogenous polyamine deficiency of the mutant resulted in marked reduction in the ArcA level determined by Western blot analysis. Putrescine supplement to the growth medium effectively increased the ArcA level of the mutant in a concentration-dependent manner. Spermidine also stimulated the ArcA level in the mutant to a greater degree than putrescine. Expression of arcA'::lacZ operon fusion in the mutant was stimulated 6-fold and 10-fold by putrescine and spermidine at a 1mM concentration, respectively, indicating that the stimulatory effect of the polyamines on ArcA synthesis is due to transcriptional induction, and that spermidine is a more potent arcA inducer than putrescine. The polyamine-dependent arcA'::lacZ induction was growth-phase-dependent and independent of either arcA or fnr which are two regulators involved in anaerobic stimulation of the Arch level. These results suggested that putrescine and spermidine polyamines may be potential intracellular signal molecules in the control of arcA expression, and thereby may play an important role in cellular metabolism.

• 비파괴검사학회지
• /
• 제26권5호
• /
• pp.285-290
• /
• 2006

고장력볼트의 체결상태를 평가하는 것은 체결볼트의 안전유지관리 측면에서 매우 중요하다. 본 논문에서는 초음파의 음속이 응력에 의존하는 이른바 음탄성효과에 기초하여 초음파의 전파시간(TOF)를 측정함으로써 볼트에 작용하는 축응력을 평가하는 방법을 제안한다. 그런데 일반적으로 볼트체결시 볼트에 작용하는 응력범위내에서의 음속 변화량은 매우 작으며, 따라서 TOF 측정에 있어서 높은 정밀도가 요구된다. 이를 위해 본 논문에서는 톤버스트파형의 초음파를 이용한 위상검출법을 적용하였다. 제안된 원리는 실험을 통해 검증되었으며, 볼트 축응력 측정의 유효성을 검증하기 위해 스트레인게이지를 이용한 축응력 측정결과와 비교하였다. 두 결과는 양호한 일치를 보였으며, 이로써 제안한 방법이 체결된 고장력볼트의 체결상태 평가에 유용하다는 결론을 얻을 수 있었다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권3호
• /
• pp.1149-1155
• /
• 2016

Background: Examination of sentinel lymph node (SLN) biopsies provides accurate nodal staging for breast cancer and plays a key role in patient management. Procurement of SLNs and the methods used to process specimens are equally important. Increasing the level of detail in histopathological examination of SLNs increases detection of metastatic tumours but will also increase the burden of busy laboratories and thus may not be carried out routinely. Recommendation of a reasonable standard in SLN examination is required to ensure high sensitivity of results while maintaining a manageable practice workload. Materials and Methods: Twenty-four patients with clinically node-negative breast cancer were recruited. Combined radiotracer and blue dye methods were used for identification of SLNs. The nodes were thinly sliced and embedded. Serial sectioning and immunohistochemical (IHC) staining against AE1/AE3 were performed if initial H&E sections of the blocks were negative. Results: SLNs were successfully identified in all patients. Ten cases had nodal metastases with 7 detected in SLNs and 3 detected only in axillary nodes (false negative rate, FNR=30%). Some 5 out of 7 metastatic lesions in the SLNs (71.4%) were detected in initial sections of the thinly sliced tissue. Serial sectioning detected the remaining two cases with either micrometastases or isolated tumour cells (ITC). Conclusions: Thin slicing of tissue to 3-5mm thickness and serial sectioning improved the detection of micro and macro-metastases but the additional burden of serial sectioning gave low yield of micrometastases or ITC and may not be cost effective. IHC validation did not further increase sensitivity of detection. Therefore its use should only be limited to confirmation of suspicious lesions. False negative cases where SLNs were not involved could be due to skipped metastases to non-sentinel nodes or poor technique during procurement, resulting in missed detection of actual SLNs.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권24호
• /
• pp.10665-10673
• /
• 2015

Background: We wished to analyse patterns of use of needle biopsy procedures by BreastScreen Australia (BSA) accredited programs to identify areas for improvement. Design: BSA services provided anonymous data regarding percutaneous needle biopsy of screen detected lesions assessed between 2005-2009. Results: 12 services, from 5 of 7 Australian states and territories provided data for 18212 lesions biopsied. Preoperative diagnosis rates were 96.84% for lesion other than microcalcification (LOTM) and 93.21% for microcalcifications. At surgery 97.9% impalpable lesions were removed at the first procedure. Of 11548 Microcalcification (LOTM) biopsied, 46.9% were malignant. The final diagnosis was reached by conventional core biopsy (CCB) in 72.46%, FNAB in 21.33%, VACB in 1.69% and open biopsy in 4.52% of lesions. FNA is being limited to LOTM with benign imaging After FNAB, core biopsy was required for 38% of LOTM. In LOTM the mean false positive rate (FPR) was 0.36% for FNAB, 0.06% for NCB and 0% for VACB. Diagnostic accuracy was 72.75% for FNAB and 92.1% for core biopsies combined. Of 6441 microcalcifications biopsied 2305 (35.8%) were malignant. Microcalcifications are being assessed primarily by NCB but 6.57% underwent FNAB, 45.6% of which required NCB. False positive diagnoses were rare. FNR was 5% for NCB and 1.53% for VACB. Diagnostic accuracy was 73.52% for FNAB, 86.29% for NCB and 88.63% for VACB. Only 8 of 12 services had access to VACB facilities. Conclusions: BSA services are selecting lesions effectively for biopsy and are achieving high preoperative diagnosis rates. Gaps in the present accreditation standards require further consideration.

• 터널과지하공간
• /
• 제15권3호
• /
• pp.177-184
• /
• 2005

우리나라의 4차로 도로터널 중 가장 긴 약 4km의 병설 터널인 사패산터널은 환경단체의 민원에 의해 당초보다 약 2년 정도 공사가 지연되어 공사 기간을 단축하기 위한 시공 효율 극대화가 절실히 요구되었다. 본 고는 공기단축을 위해 적용된 사패산 터널의 굴진장 증대 방안, 굴착 공법 개선 방법, 그리고 막장전방 예측 시스템에 대한 사례 연구이다. 터널의 굴진장을 증대시키기 위해 Bulk-emulsion 폭약과 Cylinder-cut 심발패턴이 도입되었고, 터널 굴착 단면의 변경 및 최적의 굴착순서 계획을 통해서 공사기간을 단축할 수 있었다. 또한 대단면 터널의 안전한 시공을 위해서 막장관찰, TSP 탐사 및 고성능 천공장비를 이용한 막장전방 예측 System을 적용하였다.

• 대한건축학회논문집:구조계
• /
• 제36권3호
• /
• pp.113-120
• /
• 2020

The purpose of this study is to compare the models of Deep Learning-based Convolution Neural Network(CNN) for concrete crack detection. The comparison models are AlexNet, GoogLeNet, VGG16, VGG19, ResNet-18, ResNet-50, ResNet-101, and SqueezeNet which won ImageNet Large Scale Visual Recognition Challenge(ILSVRC). To train, validate and test these models, we constructed 3000 training data and 12000 validation data with 256×256 pixel resolution consisting of cracked and non-cracked images, and constructed 5 test data with 4160×3120 pixel resolution consisting of concrete images with crack. In order to increase the efficiency of the training, transfer learning was performed by taking the weight from the pre-trained network supported by MATLAB. From the trained network, the validation data is classified into crack image and non-crack image, yielding True Positive (TP), True Negative (TN), False Positive (FP), False Negative (FN), and 6 performance indicators, False Negative Rate (FNR), False Positive Rate (FPR), Error Rate, Recall, Precision, Accuracy were calculated. The test image was scanned twice with a sliding window of 256×256 pixel resolution to classify the cracks, resulting in a crack map. From the comparison of the performance indicators and the crack map, it was concluded that VGG16 and VGG19 were the most suitable for detecting concrete cracks.

• Nuclear Engineering and Technology
• /
• 제54권8호
• /
• pp.3034-3042
• /
• 2022

The results of radioecological monitoring of the cooling pond Beloyarsk NPP (Russia) have been presented. The influence of waste technological waters of thermal and fast NPP reactors on the content of artificial radionuclides in surface waters and bottom sediments of the Beloyarsk reservoir has been studied. The long-term dynamics of the specific activity of ⁶⁰Co, ⁹⁰Sr, ¹³⁷Cs and ³H in the main components of the freshwater ecosystem at different distances from the source of radionuclide discharge has been estimated. Critical radionuclides (⁶⁰Co and ¹³⁷Cs), routes of their entry and periods of maximum discharge of radioisotopes into the cooling pond have been determined. It is shown that the technology of electricity generation at Beloyarsk NPP, based on fast reactors, has a much smaller effect on the flow of artificial radionuclides into the freshwater ecosystem of the reservoir. During the entire period of monitoring studies, the decrease in the specific activity of radionuclides from NPP origin in surface waters was 4.3-74.5 times, in bottom sediments 10-505 times. The maximum discharge of artificial radionuclides into the reservoir was noted during the period of restoration and decontamination work aimed at eliminating emergencies at the AMB thermal reactors of the first stage of the Beloyarsk NPP.

• Nuclear Engineering and Technology
• /
• 제55권2호
• /
• pp.707-716
• /
• 2023

The influence of waste technological waters of thermal and fast reactors of Beloyarsk NPP (Russia) on the accumulation of ⁶⁰Co, ⁹⁰Sr and ¹³⁷Cs in macrophytes and ichthyofauna of the cooling pond has been studied. Critical radionuclides, routes of their entry into the ecosystem and periods of maximum discharge of radioisotopes into the cooling pond have been determined. It is shown that the technology of electricity generation at the Beloyarsk NPP, based on fast reactors, has a much smaller effect on the release of artificial radionuclides into the environment. Therefore, during the entire period of monitoring studies (1976-2019), the decrease in the specific activity of radionuclides of NPP origin in macrophytes was 13-25800 times, in ichthyofauna 1.5-44.5 times. The maximum discharge of artificial radionuclides into the Beloyarsk reservoir was noted during the period of restoration and decontamination work aimed at eliminating the emergencies at the AMB reactors of NPP. The factors influencing the accumulation of artificial radionuclides in the components of the freshwater ecosystem of the Beloyarsk cooling pond have been determined, including: the physicochemical nature of radioisotopes, their concentration in surface water, the temperature of the aquatic environment, the trophicity of the reservoir, the species of hydrobionts.

• 대한토목학회논문집
• /
• 제4권4호
• /
• pp.25-36
• /
• 1984

본(本) 연구(硏究)는 강구조부재(鋼構造部材)의 안정성수준(安全性水準)을 LRFD에 의해 검토(檢討)하였다. 그리고, 우리 현실(現實)을 고려(考慮)한 목표신뢰성지수(目標信賴性指數)(${\beta}_0$)를 설정(設定)하여 이에 대한 저항(抵抗) 및 하중계수(荷重係數)를 방법(方法)으로 계산(計算)하였으며, 각(各) 도로교시방서(道路橋示方書) 공칭안전율(公稱安全率)과 이들 계산(計算)된 계수(係數)를 비교분석(比較分析)하였다. 강구조부재(鋼構造部材)의 저항(抵抗) 및 하중효과(荷重効果)에 대한 불확실량(不確實量) 산정(算定)은 Galambos-Ravindra 및 SGST의 하중(荷重) 및 저항(抵抗) 불확실량(不確實量) 해석방법(解析方法)에 따랐다. 그 결과(結果)를 요약(要約)하면 다음과 같다. 1) 현(現) 강구조부재(鋼構造部材)에 대한 목표신뢰성지수(目標信賴性指數)(${\beta}_0$)는 우리의 현실(現實)을 고려(考慮)하여 ${\beta}_0=3.5$가 적절(適切)하다. 2) 1)에 대한 강도설계(强度設計) 방정식(方程式)에 공칭저항(公稱抵抗) ${\phi}^{\prime}$, 공칭하중계수(公稱荷重係數) ${\gamma}^{\prime}$는 아래와 같다. a) 양연지지판(兩緣支持板): ${\phi}^{\prime}=0.75$, ${\gamma}_D{^{\prime}}=1.04$, ${\gamma}_L{^{\prime}}=2.08$ b) 편지지판(片支持板): ${\phi}^{\prime}=0.82$, ${\gamma}_D{^{\prime}}=1.04$, ${\gamma}_L{^{\prime}}=2.08$.

• 정보처리학회논문지C
• /
• 제15C권5호
• /
• pp.351-358
• /
• 2008

DoS/DDoS로 대표되는 트래픽 폭주 공격은 대상 시스템뿐만 아니라 네트워크 대역폭 및 프로세서 처리능력, 시스템 자원 등을 고갈시킴으로써 네트워크에 심각한 장애를 유발하기 때문에, 신속한 트래픽 폭주 공격의 탐지는 안정적인 서비스의 제공 및 시스템의 운영에 필수요건이다. 전통적인 패킷 수집을 통한 DoS/DDoS의 탐지방법은 공격에 대한 상세한 분석은 가능하나 설치의 확장성 부족, 고가의 고성능 분석시스템의 요구, 신속한 탐지를 보장하지 못하는 문제점을 갖고 있다. 본 논문에서는 MIB 정보 갱신 시점 단위로 수집된 SNMP MIB 객체 정보를 바탕으로 Support Vector Data Description(SVDD)을 이용하여 보다 빠르고 정확한 침입탐지와 쉬운 확장성, 저비용탐지 및 정확한 공격유형별 분류를 가능케 하는 새로운 시스템을 설계 및 구현하였다. 실험을 통하여 만족스러운 침입 탐지율과 안전한 False Negative Rate(FNR), 공격유형별 분류율 수치 등을 확인함으로써 제안된 시스템의 성능을 검증하였다.