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The Mechanism of Stomatal Closing by $H_2O_2$ in Epidermal Strips of Commelina communis L. (닭의장풀의 분리표피에서 $H_2O_2$에 의한 기공 닫힘기작)

  • 이준상;전방욱
    • Journal of Environmental Science International
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    • v.6 no.2
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    • pp.125-131
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    • 1997
  • The mechanism of stomatal closing in response to $O_2$ was indirectly investigated by using $H_2O_2$ which is the intermediate product of $O_2$ metabolites. Stomata in epidermal strips close in response to $H_2O_2$. The effect of $H_2O_2$ on stomatal closing was dependent on the concentration of $H_2O_2$. 10 ppm $H_2O_2$ showed a clear effect on stomatal closing and 1000 ppm $H_2O_2$ induced complete stomatal closing after the treatment of 3 hours. Stomatal closing by $H_2O_2$ in intact leaf was also observed by measuring the diffusion resistance with porometer. It was found that the stomatal closing by $H_2O_2$ was not mediated by $Ca^{2+}$, and that was a different result observed in stomatal closing by water stress. Reversely, $Ca^{2+}$ showed a great inhibition on stomatal closing. The leakage of K+ in epidermal strips was doubled in response to $H_2O_2$ when it was campared to the control. 10 ppm $H_2O_2$ decreased photosynthetic activity. Fv/Fm representing the activity of Photosystem II was reduced about 4 % in 10 ppm $H_2O_2$ and 8 % in 100 ppm $H_2O_2$ In the treatment of 1.5 hour. However, stomatal closing by 10 ppm $H_2O_2$ was reduced about 56 %. According1y, it can be suggested that stomatal closing by $H_2O_2$ is related with the decrease of photosynthetic activity, but it was chiefly induced by the change of the membrane permeability. Key words Commelina communis, stomatal closing, $H_2O_2$, $Ca^{2+}$, photosynthesis.

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Impact of Shading Net Color on Phytochemical Contents in Two Chili Pepper Hybrids Cultivated Under Greenhouse Conditions

  • Nagy, Zsuzsa;Daood, Hussein;Nemenyi, Andars;Ambrozy, Zsuzsanna;Pek, Zoltan;Helyes, Lajos
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.418-430
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    • 2017
  • The objective of this study was to understand the combined effect of shading treatment and harvest time on pungency, ascorbic acid content, market-quality pepper yield, and photosynthetic activity in a yellow 'Star Flame' and 'Fire Flame' F1 hybrid pepper cultivar (Capsicum annuum). Plants were cultivated in a greenhouse under white, red, and green shading nets and compared to those grown under unshaded control conditions. The ascorbic acid and pungency parameters were analyzed by high-performance liquid chromatographic methods. Ascorbic acid, a compound of high nutritive value in pepper, was highest in fruits gathered during the first summer harvest in both hybrids. The ascorbic acid content was found to be further increased in fruits from plants grown under white net shading in both 'Star Flame' and 'Fire Flame'. Total capsaicinoid contents in 'Star Flame' were influenced by the interaction of shading treatment and harvest time (p = 0.004), with the lowest amounts ($241.2-251.5mg{\cdot}kg^{-1}$ fresh weight) resulting from white and red shading treatments at the early autumn harvest. In 'Fire Flame', both shading treatment (p = 0.009) and harvest time (p < 0.001) affected total capsaicinoid contents. We observed significantly higher total capsaicinoid contents in control fruits compared to that resulting from red and green shading treatments at the second harvest, and that resulting from red shading treatment at the third harvest. Analysis of photosynthetic activity revealed a significant and negative correlation between Fv/Fm values and total capsaicinoid contents in both hybrids (p < 0.001). Based on the obtained results, the harvest of peppers during summer should be promoted to produce fruits with high pungency.

Distribution of Toxin Genes and Enterotoxins in Bacillus thuringiensis Isolated from Microbial Insecticide Products

  • Cho, Seung-Hak;Kang, Suk-Ho;Lee, Yea-Eun;Kim, Sung-Jo;Yoo, Young-Bin;Bak, Yeong-Seok;Kim, Jung-Beom
    • Journal of Microbiology and Biotechnology
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    • v.25 no.12
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    • pp.2043-2048
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    • 2015
  • Bacillus thuringiensis microbial insecticide products have been applied worldwide. Although a few cases of B. thuringiensis foodborne illness have been reported, little is known about the toxigenic properties of B. thuringiensis isolates. The aims of this study were to estimate the pathogenic potential of B. thuringiensis selected from microbial insecticide products, based on its possession of toxin genes and production of enterotoxins. Fifty-two B. thuringiensis strains selected from four kinds of microbial insecticide products were analyzed. PCR assay for detection of toxin genes and immunoassay for detection of enterotoxins were performed. The hemolysin BL complex as a major enterotoxin was produced by 17 (32.7%), whereas the non-hemolytic enterotoxin complex was detected in 1 (1.9%) of 52 B. thuringiensis strains. However, cytK, entFM, and ces genes were not detected in any of the tested B. thuringiensis strains. The potential risk of food poisoning by B. thuringiensis along with concerns over B. thuringiensis microbial insecticide products has gained attention recently. Thus, microbial insecticide products based on B. thuringiensis should be carefully controlled.

Flight Measurement and Analysis of VOR Signal Influence from the Long Distance Surrounding Obstacles (원거리 장애물이 VOR에 미치는 영향의 비행측정 및 분석)

  • Park, Hyeong-Taek;Hwang, Byong-Won
    • Journal of Advanced Navigation Technology
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    • v.12 no.1
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    • pp.1-7
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    • 2008
  • International Civil Aviation Organization(ICAO) adopted VOR(Very high frequency Omni-Range) as an international standard air navigation system in 1949 and recommended every country to make use of it in formulating air route and guiding take-off and landing of an aircraft. VOR is quite a useful navigation system so that more than 2,000 VORs have been installed all over the world including 39 in Korea: however, VOR signal could be easily affected by its circumstance like a mountainous area because it provides navigation information through AM and FM of VHF carrier. Therefore this study intends to investigate how much VOR performance is affected by its circumstances. For this purpose, the performance of the 10 VORs influenced by 24 obstacles are measured and analyzed by using an aircraft equipped with measuring instruments. This study may give us a feasible solution of problems related to the VOR siting in the future.

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The Effects of Muscle Contraction by Electrical Stimulation to V Wave and Median Frequency (전기자극에 의한 근 수축이 V wave와 중앙주파수에 미치는 영향)

  • Mun, Dal-Ju;Jeong, Dae-In;Lee, Jung-Woo;Jeong, Jin-Gyu;Kim, Tae-Youl;Oh, Myung-Hwa
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.4 no.1
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    • pp.27-38
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    • 2006
  • This study analyzed changes in action potential of supraspinal neuron and motor unit depending on maximum tolerance isometric contraction(MTIC) by electrical stimulation and examined influence of functional electrical stimulation (FES) on spinal neuron adaptation. It selected 40 university students in their twenties and divided into experimental groups of 25% MTIC(I), 50% MTIC I (II), 75% MTIC(III) and 100% MTIC(IV) depending on MTIC by electrical stimulation, and performed isometric contraction of plantar flexor muscle to each experimental group with given contraction for 20 times. It measured V/Mmax and MDF pre and post exercise, compared volume of contraction. 1. V/Mmax ratio showed no significant difference in comparison among experimental groups. 2. There was significant difference in median frequency of gastrocnemius and soleus in action potential motor unit according to comparison among experimental groups(p<.001). When contraction by electrical stimulation was maximum, change was greatest. This results suggest that muscle contraction by electrical stimulation was influence to action potential of spinal motor neuron system which appear optimal level though aspect and difference degree were not in accordance. Consequently, optimal stimulation level of MTIC(50%) by FES would be lead to central nerve adaptation. muscle contraction by electrical stimulation was influence highly to MDF which should be consider to fatigue of motor unit for muscle contraction by electrical stimulation.

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Analysis of Peripheral Nerve Function and Echogenicity of Skeletal Muscle in Type II Diabetic Patient's (제2형 당뇨환자의 말초신경기능 및 골격근의 Echogenicity 분석)

  • Yoon, Se-Won;Hwang, Tae-Yeun;Kim, Young-Nam;Yoon, Hee-Jong;Jeong, Jin-Gyu;Kim, Tae-Youl
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.4 no.1
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    • pp.13-25
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    • 2006
  • This study aims to suggest clinical basis of physical therapy of neuromuscular system complication in type diabetic patients through a variety II of analysis methods including echogenicity using ultrasound image and measurement of peripheral nerve function to their neuromuscular system and provide basic materials for preparing evaluation of physical therapy and intervention program. Subjects of this study were 75 type II diabetic patients between 40 and 80 years old and it obtained the following results through echogenisity and function of peripheral nerve. Incidence of neuropathy in type II diabetes was 55.8% in men and 53.1% in women, and total incidence of neuropathy was 54.7%. Echogenicity of patients with neuropathy was significantly increased compared to that of patients with neuropahty. It was also found that there were correlations between function of peripheral nerve and echogenicity of tibialis anterior and gastrocnemius muscle. In addition, it will be important for physical therapists to divide type II diabetic patients into neuropathy and myopathy and interpret and approach changes of neuro-muscular system from comprehensive side.

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Change of RIII Reflex of Primary and Secondary Hyperalgesia Site by High Voltage Pulsed Current (고전압맥동전류에 의한 일차 및 이차통각과민대의 RIII 반사의 변화)

  • Kim, Su-Hyon;Choi, Sug-Ju;Lee, Jung-Woo;Jeong, Jin-Gyu;Kim, Tae-Youl;Kim, Gye-Yeop
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.4 no.1
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    • pp.1-12
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    • 2006
  • This study conducted RIII reflex measurement to examine degree of pain depending on polarity of high voltage pulsed current of primary and secondary hyperalgesia site in hyperalgesia rat by local thermal injury. Hind paw which was injury site was taken as primary hyperalgeisa site, sole which was injury adjacent site was taken as secondary hyperalgesia site, and mechanical pain threshold, thermal pain threshold and root mean square of RIII reflex were measured. This study was conducted with control group I of hyperalgesia rat at hind paw by thermal injury and experimental groups divided into cathodal high voltage treatment group II, anodal high voltage treatment group III and alternate high voltage treatment group IV, applied active electrode of high voltage pulsed current to hind paw directly, placed reference electrode on the sole of injury adjacent site and applied pulse frequency. It measured RIII reflex and obtained the following results: Root mean square of RIII reflex at primary hyperalgeisa site was significantly reduced in group II after 2 days of hyperalgesia. Group II showed significant decrease after 5 and 6 days of hyperalgesia. Root mean square of RIII reflex at secondary hyperalgesia site showed significant reduction in group II after 6 days of hyperalgesia. Consequently it was found that application of high voltage pulsed current of hyperalgesia site reduced RIII reflex at primary hyperalgeisa site and secondary hyperalgesia site by electrical stimuli. Effects by polarity of high voltage pulsed current showed the greatest reduction of pain threshold when cathodal active electrode was used.

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The luminescent characteristics of $(Y,Gd)_2O_2S$: Eu for FED (FED용 $(Y,Gd)_2O_2S$ : Eu 형광체 합성 및 발광특성에 관하여)

  • 이병호;최진일
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.13 no.3
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    • pp.111-116
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    • 2003
  • The red emitting phosphors for low voltage cathode luminescent, fine $(Y,Gd)_2O_2$S : Eu powders were synthesized and investigated the effect of $Gd^{3+}$as sensitizer at variety of sintering temperature. The highly intense emission line of $(Y,Gd)_2O_2$S : Eu at 627 nm is attributed in the transition from $^5D_o to ^7F_2$ energy levels. It showed the maximum value at the doping level of 5 mole% of $Gd^{3+}$at $950^{\circ}C$ of sintering temperature and then, it was degraded rapidly. The mean particle size of $(Y,Gd)_2O_2$S : Eu was obtained around 1 fm and the cathode luminescent properties of (Y,Gd)$_2$O$_2$S : Eu were better than those of $(Y,Gd)_2O_2$S : Eu.

Decrement and Recovery of Maximal Isometric Contraction by Frequency during NMES (신경근전기자극 주파수에 따른 최대 등척성 수축력의 감소 및 회복)

  • Lim, Sang-Wan;Jeong, Jin-Gyu;Jung, Dae-In;Kim, Tae-Youl
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.2 no.2
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    • pp.1-12
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    • 2004
  • The purpose of this study was to determine the effect of muscle fatigue by neuromuscular electrical stimulation(NMES). Using Biodex System 3PRO(Biodex Medical Systems Inc, USA), experiment was conducted as to the normal group(I) composed of fifteen adults and the patient group(II) composed of fifteen patients with spastic hemiplegia. As to each group, maximal tolerated intensity(MTI) and maximal tolerated isometric contraction(MTIC) in electric currents yielded by low rate(20 pps) and high rate(100 pps) neuromuscular electrical stimulation and the aspects of decrease and restoration of the isometric contraction were examined, and their strength decrement index(SDI) and strength recovery index(SRI) were also calculated. 1. As for MTI in NMES, the MTI of the group II was higher than that of the group I in both low rate and high rate NMES. In comparison within group, MTI of group II was significantly higher in high rate NMES rather than in low rate NMES(p<0.05). 2. In comparison of MTIC between groups, the group I showed higher in both low rate and high rate NMES. In comparison within group, MTIC of group II was significantly higher in high rate NMES rather than in low rate NMES(p<0.01). 3. As for SDI, both groups showed highest SDI in high rate NMES, but no significant differences could be observed. 4. As for SRI, both groups showed significantly low SRI in low rate NMES(p<0.01, p<0.05), and comparison between groups showed no significant differences could be observed. These result lead us to the conclusion that muscle fatigue was influenced by frequency, high rate NMES was lower at SDI and higher at SRI on compare to low rate NMES, therefor, a further studies concerning electrical stimulation should consider differences each frequency in response to treatment.

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An Analysis of the Reliability and Validity of a Korean Version of the Mobility and Gait Assessment Tools for Patients with Stroke

  • Jang, Ho Young;Lee, Jeong-Hoon;Oh, Jung Lim;Lee, Hyun Soo;Lee, Suk Min
    • The Journal of Korean Physical Therapy
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    • v.30 no.1
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    • pp.29-34
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    • 2018
  • Purpose: This study aimed to systematically analyze the reliability and validity of Korean versions of mobility and gait assessment tools for patients with stroke. Methods: Two reviewers conducted an independent literature search and systematically reviewed the literature. Literature published until June of 2017 was searched using search engines for electronic databases. The 2 reviewers reviewed the title and abstract of each article. Among the articles examined, we read the full text of those judged to be suitable for our study. We classified the selected data into research methods and results. The determination was made through mutual agreement. Results: The reviewers selected 5 articles related to the purpose of this study. The DGI was found to be moderately associated with BBS, 10MWT, and TUG in intra-rater reliability (ICC=0.92), inter-rater reliability (ICC=0.88), and concurrent validity tests. The FGA demonstrated a high level of reliability with intra-rater (ICC=0.92-0.95) and inter-rater reliability (ICC=0.91, 0.95). The intra-rater reliability of the RMI was high at ICC=0.98. Its concurrent validity showed a high association with STREAM and BI. The intra-rater reliability of the MAS was ICC=0.75-0.99. Its inter-rater reliability was very high, exceeding 0.99. The inter-rater reliability of the Tinetti-gait Scale was ICC=0.91, and its concurrent validity was moderately associated with the DGI, 10WMT, OLST, FM-L/E, and STS. Conclusion: The results of this study revealed that the reliability and validity of the Korean versions of the mobility and gait assessment tools were high.