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Evaluation of a Mixture of Plant Protein Source as a Partial Fish Meal Replacement in Diets for Juvenile Olive Flounder Paralichthys olivaceus (식물성 단백질 혼합물을 이용한 넙치(Paralichthys olivaceus) 사료 내 어분대체 가능성 평가)

  • Kim, Min-Gi;Shin, Jaehyeong;Lee, Chorong;Lee, Bong-Joo;Hur, Sang-Woo;Lim, Sang Gu;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.4
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    • pp.374-381
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    • 2019
  • This study was conducted to examine a mixture of plant protein sources as a fish meal (FM) substitute. Two feeding trials were carried out using similar dietary formulations but different FM levels. In Experiments 1 and 2, the basal diets were formulated to contain 65% and 60% of FM, respectively. The other five diets were formulated replacing FM by 10, 15, 20, 25 and 30% with a mixture of soybean meal, wheat gluten and soy protein concentrate. Three synthetic amino acids (lysine, threonine and methionine) were added to the test diet. Groups of fish in experiment 1 ($6.76{\pm}0.03g$) and experiment 2 ($32.5{\pm}0.1g$) were fed one of the experimental diets for 7 and 9 weeks, respectively. Each experiment was carried out in triplicate. There were no significant differences among groups in terms of growth performance, feed utilization, survival or hematological parameters in either experiment. The results indicated that a mixture of soybean meal, wheat gluten and soy protein concentrate, supplemented with three synthetic amino acid, can replace fish meal by up to 30% in diets for juvenile olive flounder.

Fermentation characteristics and microbial community composition of wet brewer's grains and corn stover mixed silage prepared with cellulase and lactic acid bacteria supplementation

  • Guoqiang Zhao;Hao Wu;Yangyuan Li;Li Li;Jiajun He;Xinjian Yang;Xiangxue Xie
    • Animal Bioscience
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    • v.37 no.1
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    • pp.84-94
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    • 2024
  • Objective: The objective of this study was to investigate how cellulase or/and lactic acid bacteria (LAB) affected the fermentation characteristic and microbial community in wet brewer's grains (WBG) and corn stover (CS) mixed silage. Methods: The WBG was mixed thoroughly with the CS at 7:3 (w/w). Four treatment groups were studied: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]), iii) LAB, added LAB (2×106 cfu/g FM), and iv) CLA, added cellulase (120 U/g FM) and LAB (2×106 cfu/g FM). Results: All additive-treated groups showed higher fermentation quality over the 30 d ensiling period. As these groups exhibited higher (p<0.05) LAB counts and lactic acid (LA) content, along with lower pH value and ammonia-nitrogen (NH3-N) content than the control. Specifically, cellulase-treated groups (CEL and CLA) showed lower (p<0.05) neutral detergent fiber and acid detergent fiber contents than other groups. All additives increased the abundance of beneficial bacteria (Firmicutes, Lactiplantibacillus, and Limosilactobacillus) while they decreased abundance of Proteobacteria and microbial diversity as well. Conclusion: The combined application of cellulase and LAB could effectively improve the fermentation quality and microbial community of the WBG and CS mixed silage.

Starch characteristics of foxtail millet and sorghum cultivars grown in Korea (국내 육성 조 및 수수 전분의 이화학적 특성)

  • Kim, Hyun-Joo;Woo, Koan Sik;Lee, Byong Won;Lee, Jin Young;Lee, Yu-Young;Kim, Min Young;Kim, Mihyang;Lee, Byoungkyu
    • Korean Journal of Food Science and Technology
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    • v.52 no.3
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    • pp.220-225
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    • 2020
  • In this study, we investigated the starch characteristics of foxtail millet (FM) and sorghum (SG) cultivars grown in Korea. The amylose contents were 29.42 and 6.42% in the Daname and Samdachal FM cultivars, and 9.09 and 11.11% in the Nampungchal and Sodamchal SG cultivars, respectively. The amylopectin polymerization analysis showed that the highest degree of polymerization (DP) was in the range of DP13-24 for all samples, at approximately 60%. The resistant starch content was very low (<0.10%) among the FM and SG cultivars. The starch gelatinization analysis showed low setback values in the Samdachal and Nampungchal cultivars. The gelatinization enthalpy, calculated based on an endothermic reaction, did not differ significantly between the SG cultivars but was significantly higher in the Samdachal FM cultivars than in the Daname FM cultivars. The results of this study might be used as basic data for the development of FM and SG products.

Evaluation of Squid Liver Powder as a Dietary Protein Source Replacing Fish Meal in Juvenile Flounder, Paralichthys olivaceus (넙치 사료내 어분대체 단백질원으로써 오징어간분말의 평가)

  • Kim, Kang-Woong;Kim, Kyoung-Duck;Son, Maeng-Hyun;An, Cheul-Min
    • Journal of Fisheries and Marine Sciences Education
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    • v.23 no.3
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    • pp.461-467
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    • 2011
  • This experiment was conducted to determine the each level of a newly developed squid liver powder (SLP) as a dietary protein source replacing fish meal (FM) in juvenile flounder. A diet excluding SLP (FM diet) and a commercial diet were two control diets. Six experimental diets were formulated to replace FM with three types of SLP at 5 and 10% level, respectively (SLP-A5, SLP-A10, SLP-B5, SLP-B10, SLP-C5, SLP-C10). Prior to the start of a feeding trial, fish were fed the commercial diet for 1 week to adjust to the experimental conditions. Fish with average weight of $22.8{\pm}0.40g$ ($mean{\pm}SD$), were randomly distributed to each aquarium as groups of 20 fish and fed the experimental diets in triplicate at satiation for 8 weeks. After 8 weeks, weight gain and specific growth rate of fish fed SLP-C10 was lower than those of fish fed FM diet and SLP-B5. Meanwhile, no significant difference was observed in WG and SGR among fish fed diets excluding SLP-C10. Feed efficiency (FE) and protein efficiency ratio (PER) of fish fed each level of three types of SLP were not different from those of fish fed FM diet. However, fish fed SLP-C5 and SLP-C10 has lower FE and PER than those of fish fed a commercial diet, SLP-A5 and SLP-B5. Therefore, these results indicate clearly that dietary SLP-B could be a good protein source to develop inexpensive commercial diet in juvenile flounder.

Frequency modulation spectroscopy of a super-cavity using a single mode He-Ne laser (단일모드 헬륨네온레이저를 사용한 초공진기의 주파수 변조 분광연구)

  • 서호성;윤태현;조재흥;정명세;류갑열;김영덕;최옥식
    • Korean Journal of Optics and Photonics
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    • v.3 no.1
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    • pp.27-36
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    • 1992
  • Frequency modulation spectroscopy of the super-cavity, of which finesse is app. 40,000 has been demonstrated by using a sigle mode He-Ne laser. In-phase and quardrature components of frequency modulation signals (FM signal) were obtained by using the 1.5 MHz-driven-electrooptic phase modulator. The vector locus of the FM signa in the phase space, which is consisted of in-phase and quardrature components of the FM signal, was observed and analyzed for the dependence of FM signal upon the phase of the reference signal of a phase-sensitive-detector. According to rotating the phase of the reference signal, the vector locus was observed to rotate with the same phase angle as the reference signal. The in-phase component of the FM signals will be used to stabilize the frequency of the He-Ne laser to the resonant frequency of the super-cavity.

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Characteristics of Hemolysin Produced by Vibrio cholerae non-O1 FM-3 Isolated from Sea Water (해수분리 Vibrio cholerae non-O1 FM-3의 Hemolysin)

  • KIM Shin-Hee;PARK Mi-Yeon;LEE Young-Eon;CHO Myo-Heon;CHANG Dong-Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.4
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    • pp.556-561
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    • 1997
  • Vibrio cholerae non-O1 FM-3 was isolated from sea water, and it showed the same bacteriological characteristics as Vibrio cholerae non-O1 ATCC 25872. V. cholerae non-O1 FM-3 presented the highest hemolytic activity at stationary phase of its growth. The hemolytic activity was decreased in accordance with increasing of pretense activity of its cultural supernatant. The characteristics of the hemolysin produced by V. cholerae non-O1 FM-3 were investigated after partial purification with a Sephadex G-100 chromatography. The hemolytic activity of purified protein was stable at $4^{\circ}C$ while it was completely lost by heating at $60^{\circ}C$ for 30 min. The activity of hemolysin was increased by addition of divalent cations such as $Ca^{2+},\;Mg^{2+},\;and\;Mn^{2+}$ while it was inhibited by additions of $Zn^{2+}$. When the hemolysin was incubated with suspensions of erythrocytes at $4^{\circ}C$ and $37^{\circ}C$, respectively, hemolysis was not observed at $4^{\circ}C$ but at $37^{\circ}C$. It means that hemolysis by purified hemolysin was temperature-dependent while its binding step of hemolysin to cell membrane was temperature-independent.

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The Effect of Partial Replacement of Fish Meal by Squid Sepia esculenta Liver Powder on the Growth and Body Composition of Juvenile Black Rockfish Sebastes schlegeli (조피볼락(Sebastes schlegeli) 치어 사료에 오징어(Sepia esculenta) 간 분말에 대한 어분대체 효과)

  • Moon Lee, HaeYoung;Choi, Se-Min
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.46 no.6
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    • pp.746-752
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    • 2013
  • An 8-week feeding experiment was conducted to evaluate three types of squid Sepia esculenta liver powder (SLP) as a dietary protein source for replacement of fish meal (FM) in the juvenile black rockfish, Sebastes schlegeli. For replacement of FM, six experimental diets were formulated with 5 or 10% of either of three types (A, B, C) of SLP: SLP-$A_5$, SLP-$A_{10}$, SLP-$B_5$, SLP-$B_{10}$, SLP-$C_5$, and SLP-$C_{10}$. One of the control diets contained 100% FM as the protein source, and the other was a commercial diet (Com). Fish with an average body weight of $6.50{\pm}0.03g$ ($mean{\pm}SD$) were allocated randomly in triplicate groups of 50 to aquaria and fed the experimental diets until satiation. The weight gain (WG) and specific growth rate (SGR) of fish fed the FM, SLP-$A_5$, SLP-$A_{10}$, and SLP-$B_5$ diets were higher than those of fish fed the Com, SLP-$B_{10}$, SLP-$C_5$ and SLP-$C_{10}$ diets. No significant differences were observed in WG and SGR among the diet groups, with the exception of the SLP-$C_{10}$ diet group. The feed efficiency (FE) and protein efficiency ratio (PER) of fish fed the SLP-$A_5$, SLP-$A_{10}$, and SLP-$B_5$ diets did not differ from those fed the control diet. However, fish fed SLP-$B_{10}$ and SLP-$C_{10}$ had lower FE and PER than the fish fed the FM, commercial, SLP-$A_5$, SLP-$A_{10}$, and SLP-$B_5$ diets. Both the SLP-A and SLP-B diets, but not the SLP-C diets, replaced up to 10% and 5% of FM for juvenile black rockfish, respectively. The results of this experiment provide information that will assist in formulating an inexpensive and practical diet containing SLP for juvenile black rockfish.

Reliability and Validity of the Postural Assessment Scale for Stroke in Chronic Stroke Patients (만성뇌졸중 환자의 Postural Assessment Scale for Stroke의 신뢰도와 타당도)

  • An, Seung-Heon;Lee, Je-Hoon
    • The Journal of Korean Physical Therapy
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    • v.21 no.1
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    • pp.9-17
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    • 2009
  • Purpose: The purpose of this study was to determine reliability and validity of the Postural Assessment Scale for Stroke Patients (PASS) in chronic stroke patients. Methods: A total of 43 stroke patients, who had a stroke more than 6 months previously, participated in the study. Reliability was determined by the intra-class correlation coefficient (ICC$_{3,1}$), Bland and Altman method and Cronbach's alpha by internal consistency. Validity was examined by correlation the PASS scores to the Berg Balance Scale (BBS), Trunk Control Test (TCT), and Fugl-Meyer Balance (FM-B) score. Results: The intra-rater reliability and the Absolute reliability of the PASS was good ICC$_{3,1}$=0.97 (95%CI 0.95$\sim$0.99) and excellent SEM=1.01 respectively. Cronbach's alpha value for PASS was found to be 0.94. There were significant correlations between the PASS and BBS, TCT, FM-B (r=0.65-0.96, p<0.01). Conclusion: The PASS provide reliable and valid instrument of the postural control assessment for chronic stroke patients.

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Comparative analysis of silage fermentation and in vitro digestibility of tropical grass prepared with Acremonium and Tricoderma species producing cellulases

  • Khota, Waroon;Pholsen, Suradej;Higgs, David;Cai, Yimin
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.12
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    • pp.1913-1922
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    • 2018
  • Objective: To find out ways of improving fermentation quality of silage, the comparative analysis of fermentation characteristics and in vitro digestibility of tropical grasses silage applied with cellulases produced from Acremonium or Tricoderma species were studied in Thailand. Methods: Fresh and wilted Guinea grass and Napier grass silages were prepared with cellulases from Acremonium (AC) or Trichoderma (TC) at 0.0025%, 0.005%, and 0.01% on a fresh matter (FM), and their fermentation quality, chemical composition and in vitro digestibility were analyzed. Results: All silages of fresh Napier grass were good quality with lower pH, butyric acid, and ammonia nitrogen, but higher lactic acid content than wilted Napier grass and Guinea grass silage. Silages treated with AC 0.01% had the best result in terms of fermentation quality. They also had higher in vitro dry matter digestibility and in vitro organic matter digestibility at 6 and 48 h after incubation than other silages. Silages treated with lower levels at 0.005% or 0.0025% of AC and all levels of TC did not improve silage fermentation. Conclusion: The AC could improve silage fermentation and in vitro degradation of Guinea grass and Napier grass silages, and the suitable addition ration is 0.01% (73.5 U) of FM for tropical silage preparation.

Comparative Quality Traits, Flavor Compounds, and Metabolite Profile of Korean Native Black Goat Meat

  • Shine Htet Aung;Edirisinghe Dewage Nalaka Sandun Abeyrathne;Md. Altaf Hossain;Doo Yeon Jung;Hyun Cheol Kim;Cheorun Jo;Ki-Chang Nam
    • Food Science of Animal Resources
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    • v.43 no.4
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    • pp.639-658
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    • 2023
  • Although goat meat has several health benefits than other red meats but comsumers reluctant it due to its unpleasant flavor. This study aimed to investigate the odorant of goat meat as well as compare the quality traits of meat regarding sex status. The loin meats [non-castrated male (NCM), castrated male (CM), and female (FM)] were collected and stored at 4℃ in a laboratory refrigerator and analyzed on the 1st, 5th, and 8th consecutive days. The moisture content was the lowest and the protein content was highest in FM (p<0.05). Fat and ash content in NCM and FM were similar while lowest in CM. The CIE L* was significantly higher in NCM, but there were no significant differences of CIE a* and CIE b* within groups at initial day. The color intensity increased on the 5th storage day and decreased again after the 8th storage day, except in NCM. NCM displayed the highest thiobarbituric acid reactive substances value (p<0.05), whereas CM displayed a higher pH value than other groups throughout the storage period. Indole levels were the highest in NCM (0.031 mg/kg); however, skatole levels were not significant differences across all treatments (p>0.05). No significant difference was observed in the fatty acid composition between NCM and CM (p>0.05), whereas a significant difference was observed in FM (p<0.05). Most of the water-soluble metabolites showed significant differences between sexes. Overall, sex status effects on quality properties of meat and castration can improve the overall sensory acceptance by reducing goaty flavor of Korean native black goat meat.