• Molecules and Cells
• /
• 제46권4호
• /
• pp.231-244
• /
• 2023

Leucine-rich repeat containing 15 (LRRC15) has been identified as a contributing factor for cartilage damage in osteoarthritis; however, its involvement in rheumatoid arthritis (RA) and the underlying mechanisms have not been well characterized. The purpose of this study was to explore the function of LRRC15 in RA-associated fibroblast-like synoviocytes (RA-FLS) and in mice with collagen-induced arthritis (CIA) and to dissect the epigenetic mechanisms involved. LRRC15 was overexpressed in the synovial tissues of patients with RA, and LRRC15 overexpression was associated with increased proliferative, migratory, invasive, and angiogenic capacities of RA-FLS and accelerated release of pro-inflammatory cytokines. LRRC15 knockdown significantly inhibited synovial proliferation and reduced bone invasion and destruction in CIA mice. Runt-related transcription factor 1 (RUNX1) transcriptionally represses LRRC15 by binding to core-binding factor subunit beta (CBF-β). Overexpression of RUNX1 significantly inhibited the invasive phenotype of RA-FLS and suppressed the expression of proinflammatory cytokines. Conversely, the effects of RUNX1 were significantly reversed after overexpression of LRRC15 or inhibition of RUNX1-CBF-β interactions. Therefore, we demonstrated that RUNX1-mediated transcriptional repression of LRRC15 inhibited the development of RA, which may have therapeutic effects for RA patients.

• 한국콘텐츠학회논문지
• /
• 제10권3호
• /
• pp.423-435
• /
• 2010

세계적으로 이공계 고급인력의 배출이 감소세이고, 특히 우리나라의 경우에는 생산가능 인구 증가율과 경제활동 인구 증가율이 모두 감소하는데다가 인구 고령화의 속도도 가장 빠를 뿐만 아니라 출산률은 오히려 하향하여 미래 핵심인재 확보가 더욱 어려운 현실이다. 국내외의 각 정책이나 프로그램은 모두 외국인 인재의 유치-생활지원-활용까지의 단계별 지원책을 포함하고 있으며, 이는 다시 재정지원, 법무지원, 사회 문화적 지원의 요소별로 분류하여 수렴해볼 수 있었다. FLS조건과 Brain Internalization Process의 정책모형이 실효성(efficacy)과 활용성(applicability)을 확보할 수 있도록 시스템 사고법(Systems Thinking)을 적용하여 검증하였다. 정책모형을 둘러싼 모든 피드백 루프(feedback loop)를 인식함으로써 정책의 시행으로 발생할 수 있는 부작용을 최소화하고, 이를 위해 인과루프지도(causal loop diagram)를 활용하여 정책보완책을 모색하였다. 본 FLS 조건과 Brain Internalization Process를 활용함으로써 정부나각 대학의 외국인 인재 유치정책이 하나의 통일된 틀 안에서 체계적으로 수립 및 추진될 수 있고, 결과적으로 우리나라의 외국인 인재 유치 정책이 일관되고 표준화된 형태를 형성함으로써 외국인 인재 유치 및 활용에 대한 국제 경쟁력을 제고 하고자 한다.

• 대한조선학회 특별논문집
• /
• 대한조선학회 2017년도 특별논문집
• /
• pp.32-37
• /
• 2017

Due to the nature of semi-submergible drilling rig, various equipments are arranged in a limited space, and therefore the many types of outfitting holes passing through the hull structure are densely arranged and that is required the detailed structural strength evaluation in terms of ULS and FLS by class or client. Particularly, semi-submergible drilling rig has a variety of global load which affects the structure strength around holes compared to general commercial ship, and its response of stress is also complicated, so it is difficult to carry out the prediction design of structural strength evaluation and reinforcement. In this regard, this paper presents a case study on the evaluation of structural strength for the various holes and large openings of semi-submergible drilling rig conducted by our company, as well as an established hole verification procedure.

• Molecules and Cells
• /
• 제25권2호
• /
• pp.247-252
• /
• 2008

There are several branch points in the flavonoid synthesis pathway starting from chalcone. Among them, the hydroxylation of flavanone is a key step leading to flavonol and anthocyanin. The flavanone 3-${\beta}$-hydroxylase (GmF3H) gene was cloned from soybean (Glycine max cultivar Sinpaldal) and shown to convert eriodictyol and naringenin into taxifolin and dihydrokaempferol, respectively. The major flavonoids in this soybean cultivar were found by LC-MS/MS to be kamepferol O-triglycosides and O-diglycosides. Expression of GmF3H and flavonol synthase (GmFLS) was induced by ultraviolet-B (UV-B) irradiation and their expression stimulated accumulation of kaempferol glycones. Thus, GmF3H and GmFLS appear to be key enzymes in the biosynthesis of the UV-protectant, kaempferol.

• 대한전자공학회논문지SD
• /
• 제39권12호
• /
• pp.1016-1021
• /
• 2002

LCD backlight용 형광램프(fluorescent lamp, FL)의 전극구조에 따른 발광특성을 평가하였다. 동일한 조건에서 제작된 cold cathode fluorescent lamp(CCFL) 및 EEFL에 대하여 인가전압과 EEFL의 전극 폭에 따른 휘도와 발광효율을 측정, 평가하였다. 12 V에서 측정된 CCFL의 휘도와 발광효율은 각각 27600 cd/㎡ 및 35.3 lm/w를 나타내었다 EEFL의 휘도는 전극면적을 증가시킬수록 증가되었고, 발광효율은 전극 면적이 증가되면서 증가되었다가 전극 폭 20 mm에서 최대값을 나타낸 다음 다시 감소하였다. 20 mm의 전극 폭을 갖는 EEFL에 대하여 12 V와 14 V에서 측정된 휘도와 발광효율은 각각 21600 cd/㎡, 26500 cd/㎡ 및 35.6 lm/w, 34.8 lm/w였다.

• 농업과학연구
• /
• 제11권2호
• /
• pp.344-351
• /
• 1984

3M KCI로 면양태아피부조직(緬羊胎兒皮膚組織) 추출액(抽出液)을 제조(製造)하여 정상면양(正常緬羊)에 접종(接種)했을 때 접종(接種) 면양(緬羊)의 혈청(血淸)에는 임파구아회식능(淋巴球芽會殖能)(Iymphocyte blastogenicity)을 조지(阻止)시키는 효과(效果)가 있었다. 임파구(淋巴球)에서 보다 면양인상세포암(緬羊鱗狀細胞癌) 조직항원(組織抗原)에 감작(感作)된 임파구(淋巴球)에서 더욱 현저히 나타났다(P<0.001). Crude 3M KCI 추출액(抽出液)을 Sephadex G-150 Column으로 분획(分劃)했을 때 4개의 protein peak 가 나타났으며, 생체내(生體內)에서나 시험관내(試驗管內)에서 세포면역조지효과(細胞免疫阻止效果)를 나타내는 것은 Peak IV였다. 면양태아피부조직(緬羊胎兒皮膚組織) 추출액(抽出液)과 면양린상세포암조직(緬羊鱗狀細胞癌組織) 추출액(抽出液)의 세포면역기능(細胞免疫機能) 저하효과(低下效果)는 동일한 면역저하인자(免疫低下因子)(immunosuppressor)에 의해서 이뤄진다고 고찰(考察)된다.

• The Plant Pathology Journal
• /
• 제27권2호
• /
• pp.183-186
• /
• 2011

During the summer of 2005, specimens of soybean cultivars (Daepung, Daewon, Hwanggeum and Taegwang) showing frogeye leaf spot (FLS) were obtained from various areas in Korea. Eight isolates identified as Cercospora sojina were inoculated on the adaxial leaf surfaces of 63 Korean soybean cultivars; the disease responses to each isolate were evaluated 14 days and 21 days after inoculation. Based on the disease responses (resistant or susceptible) of the cultivars by the isolates, a set of cultivars (Anpeong, Bogwang, Cheongdu No. 1, Cheongja No. 3, Dachae, Daemang, Jangwon, Namhae, Sowon, Taegwang) were selected and inoculated with seven isolates for further testing pathogenic variance. Interestingly, 6 out of 7 tested C. sojina isolates revealed differential ability in infecting different soybean cultivars. This result may indicate the possibility of new race occurrence or pathogenic variation; this also presents evidence for prevalent FLS occurrence during humid and hot weather in Korea.

• International Journal of Naval Architecture and Ocean Engineering
• /
• 제11권2호
• /
• pp.679-687
• /
• 2019

This paper presents the autonomous swimming technology developed for an Autonomous Underwater Vehicle (AUV) operating in the underwater jacket structure environment. To prevent the position divergence of the inertial navigation system constructed for the primary navigation solution for the vehicle, we've developed kinds of marker-recognition based underwater localization methods using both of optical and acoustic cameras. However, these two methods all require the artificial markers to be located near to the cameras mounted on the vehicle. Therefore, in the case of the vehicle far away from the structure where the markers are usually mounted on, we may need alternative position-aiding solution to guarantee the navigation accuracy. For this purpose, we develop a sonar image processing based underwater localization method using a Forward Looking Sonar (FLS) mounted in front of the vehicle. The primary purpose of this FLS is to detect the obstacles in front of the vehicle. According to the detected obstacle(s), we apply an Occupancy Grid Map (OGM) based path planning algorithm to derive an obstacle collision-free reference path. Experimental studies are carried out in the water tank and also in the Pohang Yeongilman port sea environment to demonstrate the effectiveness of the proposed autonomous swimming technology.

• 동의생리병리학회지
• /
• 제20권5호
• /
• pp.1241-1244
• /
• 2006

This study was carried out to know the effect of Geopungjeseup-tang(GPJST) to on Rheumatoid Arthritis by using human fibroblast-like synoviocytes(hFLS). GPJST consists of Coisis Semen, Coccllus Trilobus DC., Lycopi Herba, Arisaematis Rhizoma. The author peformed several experimetal items : that is cytotoxicity of GPJST, mRNA expression of TNF-${\alpha}$, IL-1${\beta}$, ICAM-1, production of NO. The results were obtained as follows : The mRNA expression of pro-inflammatory cytokines TNF-${\alpha}$, IL-1${\beta}$, ICAM-1 were dose-dependently reduced in hFLS. The production of NO was reduced. Talking all these observations into account, GPJST is considered to be effective in treating rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

• IMMUNE NETWORK
• /
• 제3권3호
• /
• pp.201-210
• /
• 2003

Objective: The role of prostaglandin $E_2$ (PGE2) in the etiopathogenesis of immune and inflammatory diseases has become the subject of recent debate. To determine the role of PGE2 in rheumatoid arthritis (RA), we tested the effect of exogenous PGE2 on the production of cyclooxygenase-2 (COX-2) by rheumatoid synoviocytes. Methods: Fibroblast-like synoviocytes (FLS) were prepared from the synovial tissues of RA patients, and cultured in the presence of PGE2. The COX-2 mRNA and protein expression levels were determined by RT-PCR and Western blot analysis, respectively. The PGE2 receptor subtypes in the FLS were analyzed by RT-PCR. Electrophoretic mobility shift assay (EMSA) was used to measure the NF-${\kappa}B$ binding activity for COX-2 transcription. The in vivoeffect of PGE2 on the development of arthritis was also tested in collagen induced arthritis (CIA) animals. Results: PGE2 ($10^{-11}$ to $10^{-5}M$) dose-dependently inhibited the expression of COX-2 mRNA and the COX-2 protein stimulated with IL-$1{\beta}$, but not COX-1 mRNA. NS-398, a selective COX-2 inhibitor, displayed an additive effect on PGE2-induced COX-2 downregulation. The FLS predominantly expressed the PGE2 receptor (EP) 2 and EP4, which mediated the COX-2 suppression by PGE2. Treatment with anti-IL-10 monoclonal antibodies partially reversed the PGE2-induced suppression of COX-2 mRNA, suggesting that IL-10 may be involved in modulating COX-2 by PGE2. Experiments using an inducer and an inhibitor of cyclic AMP (cAMP) suggest that cAMP is the major intracellular signal that mediates the regulatory effect of PGE2 on COX-2 expression. EMSA revealed that PGE2 inhibited the binding of NF-${\kappa}B$ in the COX-2 promoter via a cAMP dependent pathway. In addition, a subcutaneous injection of PGE2 twice daily for 2 weeks significantly reduced the incidence and severity of CIA as well as the production of IgG antibodies to type II collagen. Conclusion: Our data suggest that overproduced PGE2 in the RA joints may function as an autocrine regulator of its own synthesis by inhibiting COX-2 production and may, in part, play an anti-inflammatory role in the arthritic joints.