• Title/Summary/Keyword: FLO8

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Temperature Sensitivity of Sigma Background Is Suppressed by the Disruption of ScKNS1 in Saccharomyces cerevisiae (ScKns1 결손에 의한 Saccharomyces cerevisiae ${\Sigma}1278b$ 균주의 온도 민감성 억제 효과)

  • Park, Yun-Hee;Park, Hee-Moon
    • Korean Journal of Microbiology
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    • v.47 no.2
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    • pp.167-169
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    • 2011
  • The Saccharomyces cerevisiae S288c strain does not show haploid and diploid filamentous growth, and biofilm formation, because it has a flo8 nonsense mutation unlike ${\Sigma}1278b$ strain which has a FLO8 gene. During the heat stress experiments to investigate the role of ScKns1, LAMMER kinase in S. cerevisiae, we found that ${\Sigma}1278b$ strain revealed heat sensitivity at $37^{\circ}C$, a mild heat stress in contrast to S288c strain. We also found that the disruption of ScKns1 and the addition of sorbitol suppress heat sensitivity of ${\Sigma}1278b$ strain. These results suggest the possibility that Flo8 and ScKns1 may interact to transducer a signal for regulating heat stress through a novel signaling pathway.

Modeling for Debris Flow Behavior on Expressway Using FLO-2D (FLO-2D를 이용한 고속도로에서의 토석류 거동 모델링)

  • Lim, Jae-Tae;Kim, Byunghyun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.39 no.2
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    • pp.263-272
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    • 2019
  • This study demonstrates the applicability of the FLO-2D for the influence analysis of the debris flow on expressway. To do this, the behavior of debris flow on the expressway was reproduced by applying the FLO-2D to actual generated debris flow. The study area is a part of the Deokyusan Service Area on the Daejon-Jinju Expressway, where traffic was blocked for 24 hours due to the debris flow in August 2005. Geographical analysis with GIS, hydrological analysis with HEC-HMS, and estimation of the amount of debris flow were carried out using field survey and soil property test data. Then, the optimum parameter combination of FLO-2D was selected through the parameter sensitivity analysis, and the behavior analysis of debris flow on expressway was applied. The comparison of the predictions with the observations shows the availability of FLO-2D for the behavior analysis of debris flow on the expressway.

Impacts of Initial Soil Moisture on Hydrologic Outflow in a Distributed FLO-2D Model (분포형 FLO-2D 수문모형에서 초기토양함수가 유출결과에 미치는 영향)

  • Lee, Khil-Ha
    • Journal of Environmental Science International
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    • v.30 no.8
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    • pp.613-619
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    • 2021
  • Soil water enters the atmosphere via evapotranspiration, where it transforms into atmospheric water vapor and plays important role in the surface-atmosphere energy exchange. Soil conditions have a direct influence on the effective rainfall, and initial soil moisture conditions are important for quantitatively evaluating the effective rainfall in a watershed. To examine the sensitivity of the initial saturation to hydrologic outflow, a two-dimensional distributed FLO-2D hydrologic model was applied to a small watershed. The initial saturation was set to 0.3, 0.5, and 0.7 and the obtained results were compared. The Green-ampt model was chosen to calculate the penetration loss. Depending on the initial soil moisture, the peak flow rate varied by up to 60%, and the total water volume in the watershed by approximately 40%.

COMPARISON OF MARGINAL MICROLEAKAGE BETWEEN LOW AND HIGH FLOWABLE RESINS IN CLASS V CAVITY (5급 와동에서 저유동성과 고유동성 레진의 변연 미세누출 비교)

  • Bae, Sang-Bae;Cho, Young-Gon;Lee, Myeong-Seon
    • Restorative Dentistry and Endodontics
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    • v.34 no.6
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    • pp.477-483
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    • 2009
  • The purpose of this study was to compare the microleakage of low and high viscosity flowable resins in class V cavities applied with 1-step adhesives. Forty Class V cavities were prepared on the cervices of buccal and lingual surfaces of extracted molar teeth and divided into four groups (n=8). Cavities were restored with AQ Bond Plus /Metafil Flo $\alpha$, G-Bond/ UniFil LoFlo Plus (Low flow groups), AQ Bond Plus/Metafil Flo and G-Bond/UniFil Flow (High flow group), respectively. Specimens were immersed in a 2% methylene blue solution for 24 hours, and bisected longitudinally. They were observed microleakages at the enamel and dentinal margins. In conclusion, the low viscosity flowable resins showed lower marginal microleakage than do the high vis cosity flowable resins in class V cavities.

Diversification of Rice Quality for Processing. Physicochemical Characteristics and Inheritance of Floury Endosperm Mutants (특수 가공용 미질개발 : 분상질배유 돌연변이 계통의 이화학적특성과 유전)

  • Kim, Kwang-Ho;Koh, Hee-Jong;Lee, Jang-Hoon;Park, Sun-Zik;Heu, Mun-Hue
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.3
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    • pp.264-274
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    • 1993
  • This study was carried out to assess the agronomic characters and physicochemical properties of floury and chalky-endosperm mutant lines induced by chemical mutagen treatment to rice varieties, Hwacheongbyeo and IR24. Linkage analysis of a floury-endosperm gene was carried out using linkage testers. The grain size of brown rice of the mutants was smaller than that of the original varieties. The l, 000-grain and 1$\ell$ weight were lighter in the mutants compared with those in the original varieties. The compound starch granules in the endosperm cell of the mutants showed a loosely-packed crystalline structure. Amylose contents in mutants ranged from 16.9 to 28.5%. Crude protein contents of the mutants were not significantly different from the original rice variety, Hwacheongbyeo, but white core mutant(line 47106) derived from IR24 showed higher protein(l1.32%) compared with IR24(8.30%). The mutants showed slightly harder gel characteristics, and much lower viscosity in Amylograph than original varieties. Steamed rice-cakes from mutant lines showed greater volume than those from original varieties. During the process of alcohol fermentation, Brix in the mutants(especially floury mutants) decreased faster and the alcohol production after 10-day fermentation was much greater in the mutants than in the original varieties. Three different gene loci for floury endosperm characteristics were identified from the allelism test among mutant lines, and the genes were tentatively symbolized as flo-a, flo-b and flo-c, respectively. A floury gene, flo-a, was linked with lg(liguleless) gene in the linkage group N, with R.V. 5.76$\pm$1.72%.

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The Study for Application of Commercial Modified Starch to Frozen and Retort Foods (냉동 및 레토르트식품에서 상업적 변성전분의 적용에 관한 연구)

  • 장재권
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.5
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    • pp.881-889
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    • 1998
  • For the application of the commercially modified starch in frozen and retort foods, apparent viscosity and water loss were measured at each stages of heating, sterilization and freezing-thawing stages. Apparent viscosity showed the exponential increase with concentration and the slopes of apparent viscosity against concentration in the Firm-Tex(hydroxypropyl distarch phosphate), Hi-Flo(acetylated distarch adipate) and Colflo 67(acetylated distarch adipate) from waxy maize starch were higher than those of Amyloacetate M20(starch acetate) and X-amylo 250(distarch phosphare) from potato starch. In the presence of 1 or 2% NaCl, X-amylo 250 among modified starches showed the increase in water loss and the decrease in apparent viscosity, whereas Colflo 67, Hi-Flo and Firm-Tex were little affected by NaCl. In the presence of 1 or 2% sucrose, water loss and apparent viscosity of the modified starches were not affected. In the range of pH 4~8, water loss and apparent viscosity of the modified starches had no change but the differences were detected to some extent between each of heating, sterilization and freezing-thawing stages. In the apparent viscosity and water loss of the modified starches after 3 week storage from heating and sterilization, Hi-Flo, Amyloacetate M20 and X-amylo 250 were not changed at the storage period, and the overall acceptability of retort food containing the modified starches such as Firm-Tex and Amyloacetate M20 were favored more than others. In the apparent viscosity and water loss of the modified starches which have been frozen and thawed three times repeatedly, Colflo 67, Hi-Flo and Firm-Tex were not changed in freezingthawing, and the overall acceptability of frozen food containing Firm-Tex was most favored.

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Sensitivity Analysis of Debris Flow Simulation in Flo-2D Using Flow Discharge and Topographic Information (유량과 지형조건에 따른 Flo-2D에서의 토석류 확산 민감도 분석)

  • Kim, Namgyun;Jun, Byonghee
    • The Journal of Engineering Geology
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    • v.32 no.4
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    • pp.547-558
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    • 2022
  • In August 2020, a debris flow occurred in Gokseon, Jeollanam-do, that resulted in the death of five residents. In this study area, high-resolution 0.03 m topographic information was generated through photogrammetry, and the amount of soil movement/loss was measured. In addition, sensitivity analysis was performed for flow depth, flow velocity, and debris flow area with the program Flo-2D using the difference in simulation parameter that discharge and topographic information. Wth increasing debris flow input discharge, increases were seen in flow depth, flow velocity, and debris flow area, as ell as in the gap in results from high-resolution topographic information and low-resolution topographic information. Also, when high-resolution topographic information was used, the results were similar to the actual (measured) flow direction of the debris flow. Therefore, the application of high-resolution topographic information increases the accuracy of the debris flow analysis results compared with low-resolution information. Results could be further imporved in the future by considering geological information such as yield stress and viscosity.

A Study on the Installation of Disaster Reduction Facility Considering Debris Flow Characteristics (토석류 유동특성을 고려한 재해저감시설 설치에 관한 연구)

  • Hang Il Jo;Kye Won Jun;Chang Deok Jang;Bae Dong Kang;Young Woo Lee
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.478-478
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    • 2023
  • 우리나라는 기후변화로 인해 태풍과 집중호우에 의한 산지재해 피해가 증가하고 있다. 이러한 산지재해를 저감하기 위해 토석류 수치모의를 하여 피해규모를 예측하거나 저감시설을 설치한다. 토석류 저감시설에는 사방댐, 방호벽, 유도수로 등이 있다. 국내에서는 사방댐을 많이 설치하고 있으나 사방댐을 설치할 시 주변 시설물의 규모와 위치를 고려해야 하는 어려움이 있다. 본 연구에서는 토석류를 저감하기 위한 시설 중 사방댐의 대안으로 방호벽을 설치하여 저감효과를 분석하였다. 2022년 8월 토석류 피해가 발생한 강원도 횡성군을 대상지역으로 선정하고 수치 지도를 이용하여 DEM자료를 구축하였다. 그리고 토석류 수치모의가 가능한 FLO-2D 모형에 적용하여 유동특성을 분석하였다. 또한 저감효과를 분석하기 위해 방호벽을 창고 주변과 주택 주변에 설치하여 해당 위치에서의 유동특성을 비교·분석하였다. 방호벽을 설치하고 토사가 퇴적되기 시작한 부분을 고려하여 설치한 후 건물 주변에서의 토석류 저감효과를 분석한 결과 창고 주변과 주택 주변에 단일로 설치하여 저감효과가 나타났으나 한계점이 나타났다. 한계점을 보완하고자 방호벽을 이중으로 설치하고 모형을 적용하여 저감효과를 비교·분석하였다.

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Safety Evalution of on the cable of Extra dosed bridges by fire (화재에 대한 Extra-dosed교 케이블의 안전성 평가)

  • Rhu, Bong-Jo;Song, Young-Sun;Park, Weon-Tae
    • Journal of the Korean Society of Hazard Mitigation
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    • v.8 no.5
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    • pp.23-33
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    • 2008
  • Extra dosed bridge among the Cabled-stayed bridges have been increasingly built in korea in recently. But such bridges were often damaged by fire due to car collison. In this study Extra dosed bridges among the cabled-supported bridges are selected to analysis model frequently to be designed and/or constructed in recent and furture in this study. COSMOS FloWorks 2007 software are used for Heat Transfer Analysis and Thermal Stress Analysis. The safety of wire, HDPE pipe and stainless steel pipe are investigated. In the case of the constant of the temperature of the heat source, the significant three variables for the analysis are selected for study : (1) the distance between the fire-proof bulk head and the heat source, (2) wind velocity, (3) the height of the end of Stainless steel pipe.