• Journal of Microbiology and Biotechnology
• /
• 제23권6호
• /
• pp.766-770
• /
• 2013

Several upstream activators required for proper activation of brlA are involved in the development, vegetative growth, toxin production, and pathogenesis of Aspergillus fumigatus. In this study, we characterized the roles of two upstream developmental regulators, A. fumigatus flbB (AfuflbB) and flbE (AfuflbE), in toxin production and virulence. The deletion of AfuflbB and AfuflbE resulted in reduction of the expression of AfulaeA. Moreover, only about 8% to 10% of fumagillin was produced in the two mutants compared with that of wild type, and ${\Delta}AfuflbB$ strain produced 85% of gliotoxin compared with wild type, whereas none was produced by ${\Delta}AfuflbB$. Flow-cytometric analysis revealed decreased necrotic and apoptotic polymorphonuclear leukocytes cell death after exposure to supernatants from ${\Delta}AfuflbB$ and ${\Delta}AfuflbB$ strains compared with the wild type. These results indicate that FlbB and FlbE are necessary for the proper laeA expression, toxin production, and virulence of A. fumigatus.

• 한국환경농학회지
• /
• 제39권4호
• /
• pp.289-296
• /
• 2020

BACKGROUND: There is a need for a revolutionary method to overcome the problem of biochar, which has relatively low adsorption capacity for existing anion pollutants, along with collectively recycling fallen leaves, a kind of forest by-product. Therefore, the objective of this study was to prepare iron-decorated biochar derived from fallen leaves (Fe-FLB), and to evaluate their adsorption properties to Congo red (CR) as anionic dye. METHODS AND RESULTS: The adsorption properties of CR by fallen leaves biochar (FLB) and Fe-FLB were performed under various conditions such as initial CR concentration, reaction time, pH and dosage with isotherm and kinetic models. In this study, Fe-FLB prepared through iron impregnation and pyrolysis of fallen leaves contained 56.9% carbon and 6.3% iron. Congo red adsorption by FLB and Fe-FLB was well described by Langmuir model and pseudo second order model and the maximum adsorption capacities of FLB and Fe-FLB were 1.1 mg/g and 25.6 mg/g, respectively. In particular, it was found that the adsorption of CR was occurred by chemical adsorption process by the outer boundary layer of Fe-FLB. CONCLUSION: Overall, the production of Fe-FLB using fallen leaves and using it as an anion adsorbent is considered to be a way to overcome the problem of biochar with relatively low anion adsorption in addition to the reduction effect of waste.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2014년도 추계학술대회 및 정기총회
• /
• pp.15-15
• /
• 2014

Fusarium graminearum (teleomorph Gibberella zeae) is an important plant pathogen that causes head blight of major cereal crops such as wheat, barley, and rice, as well as causing ear and stalk rot on maize worldwide. Plant diseases caused by this fungus lead to severe yield losses and accumulation of harmful mycotoxins in infected cereals [1]. Fungi utilize spore production as a mean to rapidly avoid unfavorable environmental conditions and to amplify their population. Spores are produced sexually and asexually and their production is precisely controlled. Upstream developmental activators consist of fluffy genes have been known to orchestrate early induction of condiogenesis in a model filamentous fungus Aspergillus nidulans. To understand the molecular mechanisms underlying conidiogenesis in F. graminearum, we characterized functions of the F. graminearum fluffy gene homologs [2]. We found that FlbD is conserved regulatory function for conidiogenesis in both A. nidulans and F. graminearum among five fluffy gene homologs. flbD deletion abolished conidia and perithecia production, suggesting that FlbD have global roles in hyphal differentiation processes in F. graminearum. We further identified and functionally characterized the ortholog of AbaA, which is involved in differentiation from vegetative hyphae to conidia and known to be absent in F. graminearum [3]. Deletion of abaA did not affect vegetative growth, sexual development, or virulence, but conidium production was completely abolished and thin hyphae grew from abnormally shaped phialides in abaA deletion mutants. Overexpression of abaA resulted in pleiotropic defects such as impaired sexual and asexual development, retarded conidium germination, and reduced trichothecene production. AbaA localized to the nuclei of phialides and terminal cells of mature conidia. Successful interspecies complementation using A. nidulans AbaA and the conserved AbaA-WetA pathway demonstrated that the molecular mechanisms responsible for AbaA activity are conserved in F. graminearum as they are in A. nidulans. F. graminearum ortholog of Aspergillus nidulans wetA has been shown to be involved in conidiogenesis and conidium maturation [4]. Deletion of F. graminearum wetA did not alter mycelial growth, sexual development, or virulence, but the wetA deletion mutants produced longer conidia with fewer septa, and the conidia were sensitive to acute stresses, such as oxidative stress and heat stress. Furthermore, the survival rate of aged conidia from the F. graminearum wetA deletion mutants was reduced. The wetA deletion resulted in vigorous generation of single-celled conidia through autophagy-dependent microcycle conidiation, indicating that WetA functions to maintain conidia dormancy by suppressing microcycle conidiation in F. graminearum. In A. nidulans, FlbB physically interacts with FlbD and FlbE, and the resulting FlbB/FlbE and FlbB/FlbD complexes induce the expression of flbD and brlA, respectively. BrlA is an activator of the AbaA-WetA pathway. AbaA and WetA are required for phialide formation and conidia maturation, respectively [5]. In F. graminearum, the AbaA-WetA pathway is similar to that of A. nidulans, except a brlA ortholog does not exist. Amongst the fluffy genes, only fgflbD has a conserved role for regulation of the AbaA-WetA pathway.

• Mycobiology
• /
• 제41권3호
• /
• pp.145-148
• /
• 2013

Vegetative growth signaling of the opportunistic human pathogenic fungus Aspergillus fumigatus is mediated by GpaA ($G{\alpha}$). FlbA is a regulator of G protein signaling, which attenuates GpaA-mediated growth signaling in this fungus. The flbA deletion (${\Delta}flbA$) and the constitutively active GpaA ($GpaA^{Q204L}$) mutants exhibit enhanced proliferation, precocious autolysis, and reduced asexual sporulation. In this study, we demonstrate that both mutants also show enhanced tolerance against $H_2O_2$ and their radial growth was approximately 1.6 fold higher than that of wild type (WT) in medium with 10 mM $H_2O_2$. We performed quantitative PCR (qRT-PCR) for examination of mRNA levels of three catalase encoding genes (catA, cat1, and cat2) in WT and the two mutants. According to the results, while levels of spore-specific catA mRNA were comparable among the three strains, cat1 and cat2 mRNA levels were significantly higher in the two mutants than in WT. In particular, the ${\Delta}flbA$ mutant showed significantly enhanced and prolonged expression of cat1 and precocious expression of cat2. In accordance with this result, activity of the Cat1 protein in the ${\Delta}flbA$ mutant was higher than that of $gpaA^{Q204L}$ and WT strains. For activity of the Cat2 protein, both mutants began to show enhanced activity at 48 and 72 hr of growth compared to WT. These results lead to the conclusion that GpaA activates expression and activity of cat1 and cat2, whereas FlbA plays an antagonistic role in control of catalases, leading to balanced responses to neutralizing the toxicity of reactive oxygen species.

• Ocean and Polar Research
• /
• 제39권4호
• /
• pp.245-255
• /
• 2017

There are a number of pieces of evidences that suggest a link between marine diatoms and microorganisms, but knowledge about related microbial communities is greatly lacking. The present study investigated the microbial community structures related to the growth of the marine diatom Cyclotella meneghiniana. We collected free-living bacteria (FLB) and particle-associated bacteria (PAB) at each growth stage (e.g., lag, exponential, stationary and death) of the diatom, and analyzed their bacterial 16S rDNA using pyrosequencing. Metagenomics analysis showed that community structures of FLB and PAB differed considerably with the progress of growth stages. FLB showed higher diversity than PAB, but variation in the different growth stages of C. meneghiniana was more evident in PAB. The proportion of the genus Hoeflea, belonging to the order Rhizobiales, was dominant in both FLB and PAB, and it gradually increased with the growth of C. meneghiniana. However, Enhydrobacter clade tended to considerably decrease in PAB. In addition, Marinobacter decreased steadily in FLB, but first increased and then decreased in PAB. These results suggest that Hoeflea, Enhydrobacter, and Marinobacter may be closely related to the growth of diatom C. meneghiniana.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1996년도 춘계학술발표회논문집(2)
• /
• pp.622-627
• /
• 1996

Detailed analysis of Feedwater Line Break (FLB) event for the fuel failure point of view are lack because the event was characterized as the increase in reactor coolant system (RCS) pressure. Up to now, the potential of the rapid system heatup case has been emphasized and comprehensively studied. The cooldown effects of FLB event is considered to be bounded by the Steam Line Break (SLB) event since the cooldown effect of SLB event is larger than that of the FLB event. This analysis provides a new possible path which can cause the fuel failure. The new path means that the fuel failure can occur under the heatup scenario because the Pressurizer Safety Valves (PSVs) open before the reactor trips. The 1000 MWe typical C-E plant FLB event assuming Loss of Offsite Power (LOOP) at the turbine trip has been analyzed as an example and the results show less than 1% of the fuel failure. The result is well within the acceptance criteria. In addition to that, a study was accomplished to prevent the fuel failure for the heatup scenario case as an example. It is found that giving the proper pressure gap between High Pressurizer Pressure Trip (HPPT) analysis setpoint and the minimum PSV opening pressure could prevent the fuel failure.

• 한국산학기술학회논문지
• /
• 제13권9호
• /
• pp.4177-4185
• /
• 2012

LED는 환경 친화적인 광원으로 에너지 절약을 위해 기존 조명등을 빠른 속도로 대체하고 있다. 최근에는 에너지 소비가 많은 빌딩의 설계와 옥 내외 광고를 제작함에 있어서 LED를 적극 채택하여 그린 빌딩 및 고효율 저비용 백라이트 도입에 박차를 가하고 있다. 특히 기존의 형광등을 이용했던 실내등과 광고판용 백라이트 분야는 전력소비가 많을 뿐 아니라 SMPS(switched mode power supply)에 의한 부피, 무게 및 수명제한의 제약이 있었다. 그러므로 본 논문에서는 SMPS가 필요 없는 $12{\times}12$ FLB(flexible LED board)와 실내등용 LED 면조명을 위한 AC 직결형 구동기를 개발하였다. 제안 시스템은 고역율의 LID-PC-R101B 칩셋을 포함하고 고효율을 위한 LED 스위치 회로들로 구성되어 있다. 정교한 시스템 디자인을 통해 고효율, 높은 안정성 및 낮은 에너지 소비의 장점을 가지게 한다. 제안된 FLB는 크기 $450{\times}450$ mm, 두께 4 mm 그리고 무게 280 g의 초슬림 구조를 가진다. 최종적으로 제안시스템의 성능검증을 위해 교류 직결형 구동 회로를 채택한 FLB와 면조명의 시제품을 제작하여 실험하였다.

• Nuclear Engineering and Technology
• /
• 제19권1호
• /
• pp.10-21
• /
• 1987

극한적인 열제거 기능 상실사고인 급수관 파열사고에 대한 불확실성 해석을 반응표면방법과 Monte Carlo모사를 이용해서 원자력 1호기에 대하여 수행하였다. 여러번의 RELAP4/MOD6를 이용한 급수관 파열사고 해석을 통해 불확실성 해석의 Data Base를 마련하였으며, 비교 목적으로 평가모형 계산도 수행하였다. 급수관 파열사고 이후의 원자로 냉각재계통 최대 압력에 미치는 영향을 조사비교하기 위해 2증류의 입력 Set에 대한 반응표면방법이 활용되었다. 첫 Set는 6개의 주요 발전소 운전변수로 구성되며, 둘째 Set는 5개 주요 모형변수로 구성된다 결과의 비교 분석을 통해 모형변수의 불확실성 이 최대 압력에 미치는 영 향이 운전변수 불확실성의 영향보다 매우 큰 것이 밝혀졌고, 최대 압력 증가의 약 9%에 해 당되는 여유도 개선도 확인되었다. 또한, 평가모델에서 인정되고 있는 초기 냉각재 노심입구 온도에 대한 가정은 잘못된 것으로 밝혀졌다.

• 전자공학회논문지CI
• /
• 제46권3호
• /
• pp.86-95
• /
• 2009

이기종 컴퓨팅 환경에서 방향성 비순환 그래프(directed acyclic graph DAG)의 효율적인 스케줄링은 시스템의 성능을 높게 만드는데 매우 중요한 역할을 한다. 이기종의 컴퓨팅 환경에서 DAG로 표현되는 프로그램의 최적 스케줄링 방법을 찾는 것은 잘 알려진 '정해진 시간 내에 해결하기 어려운 문제(NP-complete)' 이다. 본 논문은 분산 이기종 컴퓨팅 시스템에서 병렬로 실행 가능한 프로그램을 위한 새로운 리스트 스케줄링 알고리즘인 HRPS(Heterogeneous Rank-Path Scheduling)를 제안하였다. HRPS의 가장 궁극적인 목적은 프로그램의 실행시간을 최소화하는 것이다. 알고리즘의 성능을 위해 DAG 입력 그래프를 이용하여 기존에 제안되어진 CPOP, HCPT, FLB 알고리즘과 스케줄의 길이를 비교한 결과 성능 향상의 결과를 얻을 수 있었다.

• ALGAE
• /
• 제38권2호
• /
• pp.111-126
• /
• 2023

Mixotrophic dinoflagellates act as primary producers, prey, and predators in marine planktonic food webs, whereas exclusively autotrophic dinoflagellates are primary producers and prey. Species of the dinoflagellate genus Scrippsiella are commonly found in marine ecosystems and sometimes cause harmful red tides. Among the 28 formally described Scrippsiella species, S. acuminata has been found to be mixotrophic and two unidentified species have been found to be mixotrophic. To determine whether the other species in this genus are similarly mixotrophic, the mixotrophic ability of S. donghaiensis SDGJ1703, S. lachrymosa SLBS1703, S. masanensis SSMS0908, S. plana SSSH1009A, and S. ramonii VGO1053 was explored using 15 potential prey items, including 2-㎛ fluorescently labeled microspheres (FLM) and heterotrophic bacteria (FLB), the cyanobacterium Synechococcus sp., and various microalgal prey species. The ability of S. acuminata to feed on FLM and FLB was also investigated. We found that S. donghaiensis, S. lachrymosa, S. masanensis, S. plana, and S. ramonii did not feed on any potential prey tested in this study, indicating a lack of mixotrophy. However, S. acuminata fed on both FLM and FLB, confirming its mixotrophic ability. These results lowered the proportion of mixotrophic species relative to the total number of tested Scrippsiella species for mixotrophy from 100% to 29-38%. Owing to its mixotrophic ability, S. acuminata occupies an ecological niche that is distinct from that of S. donghaiensis, S. lachrymosa, S. masanensis, S. plana, and S. ramonii.